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Tutorial: Searching on NRDR 2.0

NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

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How to Use Tutorial for the Non-Coding RNA Databases Resource, an integrative resource for non-coding RNA research.

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Page 1: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Tutorial:

Searching on NRDR 2.0

Page 2: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

This tutorial shows you how to perform the following 4 searches in NRDR 2.0:

•  Searching siRNAs involved in Cancer

•  Searching human miRNAs in databases with similarity search tool

•  Searching databases containing FASTA files for download

•  Downloading all sequences available for Arabidopsis thaliana

Page 3: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 1 Searching siRNAs involved in Cancer

* Speficy cancer as a word in Overview

Page 4: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

* Select siRNA as ncRNA class

Example 1 Searching siRNAs involved in Cancer

Page 5: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

* Click here to continue

Example 1 Searching siRNAs involved in Cancer

Page 6: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 1 Searching siRNAs involved in Cancer

You should see something like this:

Page 7: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 1 Searching siRNAs involved in Cancer

* Click here to see more information about this

You should see something like this:

Page 8: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 2 Searching human miRNAs in databases with similarity

search tool

* Specify Homo sapiens as organism

Page 9: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 2 Searching human miRNAs in databases with similarity

search tool

* Select miRNA as ncRNA class

Page 10: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 2 Searching human miRNAs in databases with similarity

search tool

* Specify Similarity as search method

Page 11: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 2 Searching human miRNAs in databases with similarity

search tool

* Click here to continue

Page 12: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 2 Searching human miRNAs in databases with similarity

search tool

You should see something like this:

Page 13: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 3 Searching databases containing FASTA files for

download

* Only specify FASTA in download type

Page 14: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

* Click here to continue

Example 3 Searching databases containing FASTA files for

download

Page 15: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

You should see something like this:

Example 3 Searching databases containing FASTA files for

download

Page 16: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 4 Downloading all sequences available for Arabidopsis

thaliana

* Click on Download at the Main menu

Page 17: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Example 4 Downloading all sequences available for Arabidopsis

thaliana

* Specify Arabidopsis thaliana as your organism

Page 18: NRDR 2.0 Tutorial - The Non-Coding RNA Databases Resource Tutorial

Thank You!

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