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Agronomic Management of MLN in Maize Improvement
Stephen Mugo, Mosisa Regasa and Anne
Wangui
Presented to the Training Workshop on MLN Diagnosis and Management, Naivasha, 17-19 March
2015
Facts about MLN
MLN is a viral diseaseMLN is caused by 2 virusesMLN is transmitted by insect vectorsSeed transmission of MLN is possible Mechanically transmittedCan be transmitted through infested soil (maize residue) MLN transmission vectors can be aided by wind
MLN Disease Development
Vectors: Presence of aphids and thrips, other
insects
Environment: Conditions
favoring vectors and
disease
Susceptible Germplasm
Virus: Either individual or compound
Community Approaches for MLN Control Awareness creation to all stakeholders
Policy makers, researchers, extension staff, seed producers, farmers
Reduction of the level of inoculumMaize- free “Break” seasonsCrop rotationPlanting earlyCrop diversificationRouging diseased plants
Develop MLN tolerant germplasmTest commercial cultivars and elite germplasmInitiate MLN tolerance/resistance breeding in maize improvement programs
Maize Seed Increase/Breeding nursery
Purposes• The source of seed for the next generation• Beginning of large seed increases • The source of seed to be distributed to large areas
Desired qualities• Adequate amount of seed for planned purposes• Acceptable seed hygiene.
Maize Seed Increase/Breeding nursery
Certified seed
Pre-basic / Basic seed
Nursery
Maize Seed Increase/Breeding nursery
Nursery
Recipient level 1-1
Recipient level 2 -1Recipient level 2 -2
Recipient level 1-2
Recipient level 2 -3
Managing MLN in the Maize Seed Increase/Breeding Nursery
1. Seed source•Use clean seed
• From MLN free nursery• Certified MLN-free seed
2. Grow nursery in MLN free country region•Implement monitoring for MLN
3. Seed Treatment: treat seed with an insecticide
Managing MLN in the Maize Seed Increase/Breeding Nursery
Seed treatments● Clothianidin (Poncho),● Thiamethoxam (Cruiser), ● Imidacloprid (Gaucho)● Imidacloprid + thiodicarb (Aeris)
Demonstrated to provide early stage protection against MLN vectors such as thrips, aphids and beetles.
Experience from SARI 2014
Poncho FS600 at 0.5mg/kernel and Amigo FS275 at1.0mg /kernel seed treatment
• Equally adequately protected the crop from MLN infection via control of the aphids and thrips vector, but for only about 2 weeks after germination.
• This was confirmed from less vector counts and disease incidence in that period.
Managing MLN in the Maize Seed Increase/Breeding Nursery
Managing MLN in the Maize Seed Increase/Breeding Nursery
Seed dressing combined with foliar sprays tested in this study were effective in reducing MLN incidence at early stages of plant growth
Experience from SARI 2014Thunder OD 145 foliar spray treatment alone commencing from the 2nd week after germination was not very effective in MLN reduction but the vector control from that period was significantly reduced:
• The vector had already infected the plants• This intervention was already too late for MLN control
Managing MLN in the Maize seed production fields/ nurseries
Early planted crop
Late planted crop
Managing MLN in the Maize seed increase/breeding Nurseries
4. Ensure clean fieldI. Farm planning – First planted nursery is as far
downwind as possibleII. Crop rotation – avoid MCMV in maize residueIII. Maize-Free period – at least one month free periodIV. Alternative MLN hosts - avoid around the fieldV. Avoid volunteer maize plants
Managing MLN in the Maize seed increase/breeding Nurseries
Maintaining Kiboko breeding and seed production fields MLN-free through “Maize0-free” periodsTarget: At least one-month maize free period with 5km
radiusApproach: Seasonal stakeholders meeting (MoA, County
Govt, CIMMYT, KALRO, Community leaders, farmer group leaders etc)
Experiences: - Three stakeholders meeting- Rainfed farmers respected- Irrigation farmers at first ignored but learnt bitter
lesson- Kiboko has remained MLN-free- Provided forum for research and farmers to
exchange views
Managing MLN in the Maize Seed increase/ Breeding Nursery
5. Control MLN in nurseries5.1 Monitor for viral diseases
• Daily monitor, record, rogue out, and destroy plants with viral symptoms
• If in a new area – send samples for viral diagnostic verification.
5.2. Vector monitoring• Weekly monitor for vectors (thrips/beetles)• Erect insect sticky traps
Managing MLN in the Maize seed increase /breeding Nurseries
6.3. Control MLN in nurseries
• Spray the first spray immediately after germination• Spray up to physiological maturity• Spray insecticides every two weeks
Ø Change chemistries over time• to control all vector species• avoid development of resistance among vectors.
Weekly pesticides for MLN preventionKiboko, Kenya
Pesticide Active ingredient
Group Target pests Mode of action
Marshal 250 EC Carbosulfan250g/l
Carbamate Mites, Thrips, Aphids, Leaf hoppers and Maize stalk borer
Systemic
Duduthrin 1.7 EC
Lambda cyhalothrin17.5g/l
Pyrethroid Thrips, Aphids, Mites, Leaf miners & Caterpillars
Contact
Actara 25 WG Thiamethoxam 250g/kg Neonicotinoid
Aphids,Thrips, Whiteflies and Leaf hoppers
Systemic
Thunder OD 145
Imidacloprid 100g/L + Beta-cyfluthrin 45g/l
Neonicotinoid + Pyrethroid
Thrips, Aphids & Whiteflies
Contact + Systemic
Weekly pesticides for MLN preventionKiboko, Kenya
Pesticide Active ingredient
Group Target pests Mode of action
Confidor 200 SL Imidacloprid200g/L
Neonicotinoid Whiteflies, Aphids, Thrips
Systemic
Methogun 90 SP Methomyl 90% w/w
Carbamate Thrips,Aphids Contact + Systemic
Bulldock star EC Beta- cyfluthrin12.5 g/l + Chlorpyrifos250 g/l
Pyrethroid + Organophosphate
Aphids,Caterpillars,Whiteflies,Mites, bollworms
Contact
Colt 480 EC Chlorpyrifos480g/l
Organophosphate
Aphids,Caterpillars,Whiteflies,Mites
Contact
Weekly pesticides for MLN preventionKiboko, Kenya
Pesticide Active ingredient
Group Target pests Mode of action
Twiga ace20% SL
Acetamiprid200g/L
NeonicotinoidAphids, Whiteflies Systemic
Cyclone 505 ECCypermethrin10% w/v + Chlorpyrifos35%w/v
Pyrethroid + Organophosphate
Aphids, bollworms, armyworms
Contact
Pyrinex 48 EC Chlorpyrifos480g/l
Organophosphate
Aphids,Caterpillars,Whiteflies,Mites
Contact
Calypso Sc 480 Thiacloprid480g/L
NeonicotinoidAphids, Thrips and Whiteflies
Systemic
Weekly pesticides for MLN preventionKiboko, Kenya
Weekly pesticides for MLN preventionKiboko, Kenya
Managing MLN in the Maize Breeding Trials
1. MLN data not required from trial● Treat like the nursery
2. MLN data required from trialI. Natural inoculation with MLN
● Do not apply seed treatment● Do not control vectors● Treat and take data as presented in this meeting
II. Artificial inoculation with MLN● Do not apply seed treatment ● May wish to control vectors to avoid spread of viruses to
neighboring fields.● Treat and take data as presented in this meeting
23
We need to increase the pool of MLN resistant germplasm
Questions
1. What have you done in your country
Thank you