INDUSTTRIAL PRESENTATIONPLANT TISSUE CULTURE

BY:VIVEK KUMAR CHAURASIA

BT (VII) SEMESTER1306854073

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CONTENT‽ Introduction about Plant Tissue Culture‽ Basic Apparatus needed for PTC Lab‽ Basic Media in PTC : MS Media‽ Basic Protocol of Plant Tissue Culture‽ Plants Cultured:

a. Aloe Vera

b. Potato

‽ Results‽ Bibliography

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INTRODUCTION TO PTC Plant Tissue Culture (PTC) is the field of science in which we generate an entire plantlet under the controlled conditions (in vitro) and then introduced in the environment.

It’s a rapid process as compared to the conventional plant growing procedure and any plant part can be used to generate the entire plant.

The property of the plant which is the principle of the PTC is totipotency (ability to generate entire plant from a single part)

However, not all plant cells show totipotency, therefore cells from meristem or younger cells are used for producing culture.

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APPARATUS NEEDED FOR PTC LAB

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Hot Air Oven (For glassware sterilization)

Magnetic Stirrer (for mixing media components)

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pH balance (for maintaining the pH )

Autoclave (for sterilizing the media)

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Laminar air flow (for inoculation and sub culturing of plantlets)

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BASIC MEDIA : MS MEDIA

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BASIC PROTOCOL OF PTC

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• Sterilization of glasswares (jars,beakers, test tubes etc)• The glasswares are washed and are then either dried in oven or autoclaved

• Preparation of media • Mostly MS media is used with some modifications

• Sterilization of media (Autoclaving of media )• Thermo labile components should be sterilized separately through filtration and then added later

• Washing of explant• This includes washing explant with tap water, distilled water, detergent, ethanol

• Inoculation of explant in the media• The inoculation is done in the laminar air flow under sterile conditions

• Incubation• The jars or test tubes after inoculation are kept in the growth room under optimal temperature,

light (photoperiod), humidity etc to support the growth of the explant

• Sub Culturing• The explants on which growth is noticed are sub cultured i.e. transferred to different media jars.

This ensures the nutrients for the growing plantlet.

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PLANTS CULTURED

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ALOE VERA

Sceintific name : Aloe barbadensis miller.

Explant used : Young axillary shoots

Media Used : BAP media

Conditions : 22± 2˚ C, 16/8 hours photoperiodOf incubation

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Inoculated jars of aloe vera

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POTATOScientific name : Solanum tuberosum

Explant used : Eye of the potato (tuber)

Media used : MS media

Conditions : 18± 2˚ C, 18 hours photoperiodOf Incubation

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Inoculated jars of potato tubers

RESULTS

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After 40-45 days of inoculation, the callus growth was

Observed in the Aloe Vera jars

These callus were then sub cultured in the new jars.

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After 45 days of culture, the growth was observed in potato jars.

They were subsequently sub cultured

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BIBLIOGRAPHY www.slideshare.net

www.Wikipedia.org

www.pubmed.com

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THANK YOU