Upload
araport
View
171
Download
3
Tags:
Embed Size (px)
Citation preview
araport.org @araport
A Guided Tour of Araport
Your “One-Stop” Data Shop
Agnes Chan J Craig Venter Institute
July 7, 2015
araport.org @araport
Araport • 2012: A Community White Paper published
in Plant Cell • 2013: Funded by the US National Science
Foundation (NSF) • Goal: An Arabidopsis Data Store,
– “Used” by the Community – “Built-upon/Extended” by the Community
araport.org @araport
www.Araport.org
araport.org @araport
Araport
Warehousing
Users
Chado
JBrowse
Science Apps
Custom analysis
Real-time Federation
ThaleMine Gene List Analysis
Gene Report
Query, Web Services
Data Sources
Biologists/ Bioinformaticians
Software Developers
araport.org @araport
What can you find in Araport? • Col-0 Genome Annotation
– Araport11 Genome Annotation (pre-release) – Web Apollo Community Annotation Tool
• Data & Tools – ThaleMine Data Warehouse – JBrowse Genome Browser – Webservices and Science Apps Store
araport.org @araport
Community Co-expression
(ATTED)
ePictographs (BAR)
Real-time
Data Centers
Community Interactions
(BAR)
Array expression (BAR)
Publications (NCBI, UniProt)
Pathways (KEGG)
TAIR10 • Gene symbols • Curator summaries
ThaleMine Gene List Analysis
Gene Report
Query, Web Services
ThaleMine – Data warehouse
Warehouse
Araport
Data Sources
araport.org @araport
ThaleMine
araport.org @araport
ThaleMine – Gene Report Explore & Export Data Tables
Data Summary
Categorical Summary
Numerical Summary
araport.org @araport
ThaleMine – Germplasm (Demo) Links to Genotype and Phenotype
araport.org @araport
ThaleMine – Gene List Analysis
araport.org @araport
ThaleMine – Gene List Analysis Test for Functional Enrichments
Chromosome Distribution GO Enrichment Pathway Enrichment
araport.org @araport
ThaleMine – Data Queries
araport.org @araport
ThaleMine – Data Queries Custom Export Selected Data
araport.org @araport
ThaleMine – Data Queries Export GO terms for a Custom Gene List
araport.org @araport
ThaleMine – Web Services Tools -> Web Services -> ThaleMine API
araport.org @araport
ThaleMine – Web Services Programmatically access any data you see
araport.org @araport
Community Data
Tools
Science Apps
Araport
Science Apps
Custom analysis
qRT-PCR GFP images
Demo 1 (AtExpress Profiling)
Expression study of 1000 hypothetical
genes
JCVI
Real-time
araport.org @araport
Demo Science App GFP Expression for Hypothetical Genes
araport.org @araport
Community 1001 Genomes
Variants (Ensembl)
Epigenetic (EPIC-CoGe)
Real-time
Araport11
Community TDNA-seq
Over 70 tracks
TAIR10 • Gene models, etc.
JBrowse – Genome Browser
Warehouse
Araport
Data Sources
• Updated gene models • RNA-seq expression
by tissue
JBrowse
araport.org @araport
JBrowse View Gene Models with
TDNA-seq, RNA-seq, 1001 Genomes Variants,
araport.org @araport
JBrowse Browse 1001 Genomes Variants
araport.org @araport
JBrowse Browse 1001 Genomes Variants
araport.org @araport
JBrowse Browse 1001 Genomes Variants
araport.org @araport
JBrowse Upload your Own Track
• File > Open
araport.org @araport
Araport11 Pre-release Col-0 Genome Annotation
Araport11 Protein Coding Genes
UniProt Update
NCBI Novel
Updated Transcripts
Novel Transcripts
No Change Transcripts
Maker Novel
NCBI SRA RNA-seq
PASA Update
Trinity Assembly
~1,200 ~16,000 ~26,000
araport.org @araport
Araport11 Pre-release View Araport11 Gene Models in JBrowse
araport.org @araport
Araport11 Pre-release Download GFF/FASTA Files
• Data -> Downloads
araport.org @araport
Araport11 Pre-release Web Apollo Community Annotation
araport.org @araport
Araport11 Pre-release Edit and Curate Gene Models
Gene Edit Form
araport.org @araport
www.Araport.org
Gene Report Custom Queries
Araport11 annotation GFF/FASTA
araport.org @araport
Araport iPad Competition Science Apps Ideas Challenge
(Summer 2015) • Describe a use-case-driven
App Idea. • Your idea would integrate
one or more data sources, and/or perform analysis.
How to Submit Your Ideas? • Submission details at
https://www.araport.org. • Opens through August 31,
2015.
Win an iPad
araport.org @araport
And More… Android App with ThaleMine
• Download “Intermine” from Google Play Store
• Start searching for your favorite genes (e.g. FT, ABI3)
Araport Poster •Talk 006, near the left exit of Blue Amphi (Systems Biology and New approaches)
•Online demonstration
Android App for ThaleMine
Developers’ Workshop Fall 2015
araport.org @araport
Acknowledgements J Craig Venter Institute • Chris Town • Jason Miller • Agnes Chan • Erik Ferlanti • Vivek Krishnakumar • Irina Belyaeva • Maria Kim • Chia-Yi Cheng • Seth Schobel
University of Cambridge • Gos Micklem • Sergio Contrino Former members • Ben Rosen • Svetlana Karamycheva • Eleanor Pence
Texas Advanced Computing Center • Matt Vaughn • Steve Mock • Rion Dooley • Matt Hanlon • Joe Stubbs • Walter Moreira • Chris Jordan
TAIR • Eva Huala • Bob Muller