IDENTIFICATION OF CELLS

Small Lymphocyte

• 7-8 microns

• Round or kidney-

shaped nucleus,

diffusely lumpy to

compact chromatin

structure

• Narrow cytoplasm

edge, light blue

Large lymphocyte

• 9-15 microns

• Oval/bulging nucleus,

somewhat looser chromatin

structure than the small

lymphocyte. No nucleolus

• Plentiful colorless to light

blue cytoplasm

Neutrophil

• 12-15 microns

• Nucleus with coarse,

clumped chromatin

structure with 3-5

segments

• Plentiful pale pink to

colorless cytoplasm

• Moderate to plentiful very

fine pink granules

Eosinophil

• 12-17 microns

• Nucleus with coarse,

clumped chromatin

structure with 2-3

segments

• Cytoplasm nearly

invisible due to the

granulation,

• Plentiful large, round

brick-red granules

Monocyte

• 12-24 microns

• Kidney-shaped, bulging

or segmented nucleus.

• Plentiful, grey cytoplasm

• Possible powder-fine,

violet granules

• Vacuoles often appear in

the cytoplasm

Basophil

• 10-15 microns

• Nucleus with diffuse,

clumped chromatin

structure with 3-4 poorly

defined segments

• Plentiful pale pink to

colorless cytoplasm

• Moderate to plentiful,

unevenly sized, round,

bluish-black granules

Atypical lymphocyte/Reactive

lymphocyte

• Size about - 15 - 30

µm or more

• Nucleus: Large

irregular

• Cytoplasm: Plentiful,

deep blue color

• Moulding of the

adjacent RBCs

Myeloblast

• 10-20 microns

• Large round to oval

nucleus

• Fine evenly stained

chromatin structure

• 2-5 nucleoli

• Relatively small to

moderate amount of

pale blue cytoplasm with

clear zone closest to the

nucleus

Macrocytes

• Erythrocytes larger than

8.5 microns in diameter

are called macrocytes.

• They are thicker and

stain deeper than

normocytes.

• They are found in

megaloblastic anemias,

liver disease, hemolytic

anemias and

sometimes in diabetes.

Microcytes

• Erythrocytes smaller

than 6.5 microns are

called microcytes.

• They can be seen in

iron deficiency anemia,

Sideroblastic anemia,

thalassemia etc.

Spherocyte

• Small deeply staining

cells without central

pallor.

• They are found in

Hereditary

spherocytosis and in

hemolytic anemias.

Target Cells

• Target cells, also

known as Codocytes.

• They have the

hemoglobin

concentrated in the

middle and the

periphery of the cell,

and thereby

resemble targets.

• They can be seen in

thalassemias, iron

deficiency anemia

and in liver disorders.

THANK YOU

• SYED BASHEER UDDIN