1. Powerpoint Templates Page 1 Powerpoint Templates GELLAN GUM HYDROGEL WITH POMEGRANATE EXTRACT FOR WOUND HEALING APPLICATION NURUL SYAZDIANA BINTI MOHD ZUKI (UK26258) BACHELOR OF SCIENCE (ANALYTICAL AND ENVIRONMENTAL CHEMISTRY)

2. Powerpoint Templates Page 2 Gellan Gum (GG) has been widely used nowadays. It has features: Anionic, high molecular weight, temperature- dependent and cation-induced gelation. INTRODUCTION 3. Powerpoint Templates Page 3 Pomegranate or the scientific name is Punica granatum L. is from the family of Punicaceae. In Malay is called Delima Features: Anti-microbial, anti-viral, anti-cancer, potent antioxidant, and anti-mutagenic effects of the fruit. Extract of the pomegranate peel will be used together with GG hydrogel to produce the application for wound healing. INTRODUCTION 4. Powerpoint Templates Page 4 OBJECTIVES To obtain the extract of the pomegranate peel. To determine the characteristics of the pomegranate peel extract. To produce the gellan gum hydrogel with pomegranate extract. 5. Powerpoint Templates Page 5 LITERATURE REVIEW Antimicrobial activity of Pomegranate peel extracts Comes from these three; polyphenols, tannins, and flavonoids. Polyphenols obtained most attention because of its variety biological functions. Tannins can fight with microbial such as pathogen to get enhances skin. 6. Powerpoint Templates Page 6 LITERATURE REVIEW Antioxidant and antimutagenic activities of Pomegranate peel extracts Contains high amounts of polyphenols such as ellagic tannins, ellagic acid and gallic acid for antimutagenic activity. Antioxidant activity can prevent mutation and cancer because they can scavenge the free radical and encourage antioxidant enzymes. 7. Powerpoint Templates Page 7 LITERATURE REVIEW Inhibitory effect of pomegranate peel extracts against the mutagenicity. 8. Powerpoint Templates Page 8 LITERATURE REVIEW Pomegranate peel extracts give potential anti- inflammatory and anti-infective effects Has phenolic compounds with the flavonoids such as anthocyanins, catechins. Also has hydrolyzable tannins such as punicalin, pedunculagin, punicalagin, gallic and ellagic acid. These all account about 92% of the antioxidant activity of the fruit. Gallic acid, ellagic acid, and punicalin also featured antibacterial against intestinal flora, certain enteric pathogens for instance Escherichia coli, Salmonella spp. Shigella spp., as well as Vibrio cholerae. 9. Powerpoint Templates Page 9 Phenolic compounds in pomegranate peel Anthocyanin Catechins Punicalin Ellagic acid Punicalagin 10. Powerpoint Templates Page 10 LITERATURE REVIEW Wound healing potential Antioxidant immunity characteristic is the important activity (A) Shaving, (B) wound creation under anaesthesia, (C) medication using cetrimide-based cream (left) and pomegranate peel ointment (right) which is shown next and (D) the ointment formulated using pomegranate peel. 11. Powerpoint Templates Page 11 METHOD By using Soxhlet technique: Powdered it whether by grinding or mashing Pomegranate peel will be dried under the sunlight Extraction with the Soxhlet extractor using EtOaC, acetone, MeOh, and water for about 4 hours each The extract will be filtered through Whatman No. 41 filter paper Concentrated under vacuum in 40C 12. Powerpoint Templates Page 12 METHOD By using Reflux technique: The extract will be cooled and filtered through cheesecloth The residue will be refluxed again for an additional hour The extract then will be centrifuged at 4500g for 20 minutes Being freeze- dried to form powders Stored at 4C until further use 100g pomegranate peel to be refluxed with distilled water (1000ml) for 1 hour 13. Powerpoint Templates Page 13 METHOD Soxhlet Reflux 14. Powerpoint Templates Page 14 EXPECTATION RESULT The extract of the peel will be in powder form before being used in further use. About 80% methanolic extract of pomegranate peel has high potent inhibitor for microbials. The higher concentration of the extract, the higher the antioxidant capability will be showed up. For anti-mutagenicity test, the expected result is that the inhibition of mutagenicity of the sodium azide by the test sample will be found. The compound called punicalin, punicalagin, strictinin A and granatin B were the anti-inflammatory components. 15. Powerpoint Templates Page 15 REFERENCES Al-Zoreky, N. (2009). Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. International Journal of Food Microbiology, 134, 244248. Ismail, T., Sestili, P., & Akhtar, S. (2012). Pomegranate peel and fruit extracts: A review of potential anti-inflammatory. Journal of Ethnopharmacology, 143, 397-405. P.S. Negi, G.K. Jayaprakasha, B.S. Jena. (2003). Antioxidant and antimutagenic activities of pomegranate. Food Chemistry, 80, 393397. Yunfeng Li, C. G. (2006). Evaluation of antioxidant properties of pomegranate peel extract. Food Chemistry, 96, 254260. Sweetie R. Kanatt, M. R. (2012). Active chitosane polyvinyl alcohol films with natural extracts. Food Hydrocolloids, 29, 290-297. 16. Powerpoint Templates Page 16 GANTT CHART 17. Powerpoint Templates Page 17