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TDM OF DIGOXIN
By,
Surya Prakash singh
ROLL: 12117D3003
pharmD IV th year
IT'S A CARDIAC GLYCOSIDETHE SOURCE'S ARE DIGITALIS LANNATA DIGITALIS PURPUREAITS STRUCTURE
Basic cardenolide with three digitoxose untis bound to streroidal ringby a glycosidic linkage.
DRUG PROFILE OF DIGOXIN
DOSE AND INDICATION
DOSE:
Tablet : 0.125 - 0.25 mg TABInjection solution : 0.1mg/ML - 0.25mg/ML
INDICATION:
heart failure & atrial fibrillation.
ABSORPTION
BA: 55-90%ONSET OF ACTION PO : WITHIN 2 HR IV : WITHIN 30 MINDURATION OF ACTION : 3-4 DAYSPEAK PLASMA TIME : 1 HR
DISTRIBUTIONPROTEIN BOUND: 20-25% VD : 4 – 7 L/KGMETABOLISMLIVERELIMINATIOINT 1/2 : 1-3 DAYS EXCRETIONURINE : 80%FEACES: 10-15%
MECHANISM OF ACTION OF DIGOXIN
OTHER EFFECTS :
Neuro hormonal effects• Decrease sympathic out flow• Increase parasympathetic outflow• Improve baro receptor functioning
DECREASE HEART RATE
(NEGATIVE CHRONOTROPIC
EFFECT)
REASONS FOR TDM OF DIGOXIN ??????
METHOD OF ASSAY
Radioimmunoassay (RIA) involves the quantitation of drug using a radioactive probe by measuring the radioactivity correlated to amount of drug.The technique was introduced in 1960 by Berson and Yalow
Mixture of radioactive labeled antigen "hot"
+ antibody
Adding the sample unlabeled antigen (drug)"cold"
The unlabelled antigen displaces the labeled ones
(Ag-Ab)Complexes are removed
Supernatant remains are analyzed
Gamma Counter
From these data, a standard binding curve, like the one shown in red, can be drawn.
OTHER METHODS OF ASSAYS
Nucleic acid labeling Fluorescence labeling ELISA High throughput screening wells coated with
antibodies.
THANK YOU