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At airmid healthgroup ltd our mission is to prevent ill health caused by exposure to indoor air pollutants. To achieve this goal we have assembled a unique multidisciplinary team of Clinicians, Industrial Hygienists, Occupational Health Advisers, and Specialist Laboratory Scientists in Mycology, Virology, Bacteriology, Allergy and Immunology. Contact Graeme Tarbox email: [email protected] website: http://www.airmidhealthgroup.com To help clients with products and services for the built environment improve their customer offerings through health related innovations and marketing. The Airmid mission is to create value through a number of collaborative strategies including field research, environmental test chamber studies and licensing our own intellectual property. By specialising in studying how allergens, viruses, and other ultra-fine particles spread illness and disease in buildings we are a leading authority in aerobiology and bio-medical research. Our clients succeed by making the built environment as healthy as possible.
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www.airmidhealthgroup.com
Overview of the allergen laboratory
services and facilities
Some of the Allergen Lab Team
airmid healthgroup Facilities
3 Climate-Controlled Environmental ChambersBed BugsAllergenEnvironmental
airmid healthgroup Facilities
Class II Microbiology LaboratoryMycologyBacteriologyVirology
airmid healthgroup Facilities
Allergen Laboratory ISO/IEC 17025:2005 INAB accredited testing laboratory
Reg. no. 284T for House Dust Mite and Cat Allergen analysis
Customised studies to assesProductsEnvironments IndustryHomesBiological Samples
Live House Dust Mite Studies
airmid healthgroup is an ISO/IEC 17025:2005 INAB accredited testing laboratory Reg. no. 284T for house dust mite (Derp1) and cat
allergen (Feld1)
World’s First Allergen ISO 17025 Accredited Facility
Industries Served
Air Conditioners Air Cleaning Device Vacuum Cleaners
Textiles Surface Technologies Filters
Cleaning Products Floor Coverings
Environments
Surface Allergen Sampling – Mitest Instrument
Environmental Field Studies in USA & Europe• Assess the impact of products or services• Quantify level of reduction of bio-aerosols in “real”
situations
Air Sampling – Sidekick Pump
Live House Dust Mite Studies
Test DescriptionBespoke tests are carried out to suit the client’s needs, examining different time points. Test and control specimens are inoculated with the test organism and nutrients. After the required period of incubation in dedicated chambers, the dust mites are recovered from the specimens and analysed by heat extraction to measure live house dust mite counts or ELISA to measure the level of allergen. Results are expressed as per cent reduction on the treated sample versus the untreated control.
ObjectiveTo evaluate the degree of anti-house dust mite activity, dust mite accumulation and dust mite removal properties in a long-term testing environment for a wide variety of products including textiles, cleaning agents, repellents and appliances.
Customer BenefitThe data generated by this test provides a good measurement of the effectiveness of anti-dust mite treatment on a breeding mite colony. We can also supply image and video footage of the test.
Third Party ValidationOn passing this test, the product can be labelled as having been tested by airmid in support of any claim relating to the test and data received.
Receive Samples to Laboratory
Samples Received to the LaboratoryMitest FilterDust samples are collected from carpets and soft furnishing using a mitest instrument and filter. The mitest instrument fits on the nozzle of a vacuum cleaner and the samples are collected by vacuuming a defined area for a fixed amount of time. The mitest filter is removed and sent to the laboratory.
Fibre FilterThe fibre filter is placed in a cassette which is attached to the sidekick pump. Airborne allergen is drawn onto the fibre filter through the vacuum at a defined flow rate for a fixed amount of time.
HEPA & Air FiltersWhen a filter is challenged to remove a defined amount of allergen from the air the filters can be sent to the laboratory to quantify the level of allergen .
TextilesThe allergen barrier efficiency of a fabric can be measured using the Allergen Barrier Test with Airflow and the Simulated Use Allergen Barrier Test.
Receive Samples to Laboratory
Allergen Sample
Extraction
Allergen Sample Extraction
1. Add PBS-Tween Buffer2. Rotate for 2 hours3. Centrifuge4. Transfer supernatant
Quantify Allergen Levels
2. 3. 4. 1.
Receive Samples to Laboratory
Allergen Sample
Extraction
Measurement of Allergen
An Enzyme Linked Immunosorbent Assay (ELISA) is a highly sensitive and specific test that uses an enzyme linked antibody for the detection of a specific antigen.
Measurement of Allergen Levels
Wash Wash Wash YHRPHRP
Y YHRP
HRP
TMB
H2SO4
Monoclonal antibody – coated well. Blocking buffer is added to block remaining protein binding sites.
Antigen binds to capture antibody
A second biotinylated monoclonal antibody binds to the bound antigen.
HRP-Conjugated Streptavidin binds to biotin labelled detection antibody
TMB substrate is added and converted by enzyme into coloured product. The rate of colour formation is proportional to the amount of allergen present.
WashY
Allergen Levels are quantified for: House Dust Mite Allergen
Der p1 (D. pteronyssinus) Der f1 (D. farinae)Mite Group 2
Animal Allergen Fel d1 (Cat) Can f1 (Dog) Rat n1 Mus m1
(Rat Urine) (Mouse Urine)
Airborne Allergen Levels are quantified using an augmented ELISA for House Dust Mite and Cat Allergen.
Allergen Levels are quantified for Cockroach Allergen
Bla g1 (B. germanica)
PollenPhl p5 (Timothy Grass)Bet v1 (Birch)Amb a1 (Ragweed)
Avian Allergen (Feather & Down)Avian IgGAvian Albumin
Receive Samples to Laboratory
Allergen Sample
Extraction
Measurement of Allergen
Report Results
Report Results
Contact Usairmid healthgroup
Europe+353-1-633 6820Trinity Enterprise Campus, Grand Canal Quay, Dublin 2, Ireland
USA +1 646 291 8937345 Park Avenue,17th Floor, New YorkNY 10151-0037, USA
www.airmidhealthgroup.com/efs
Graeme [email protected]
