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Nutritional requirement Growth Metabolism Serum Topoinhibition Source substitute pH requlation
presented by : ANMOL FATIMA
Nutrients components in foods that a cell uses to survive and grow are known as nutrients
NUTRITIONAL REQUIREMENT For mammalian cell and plant cell culturing
Amino acids (arginine, leucine etc) Vitamins ( riboflavin, biotin, vitamin
B12) Inorganic ion ( sodium ,potassium, calcium) Trace elements (molybdenum, copper) Monosaccharide ( sugar ,glucose, galactose )
NUTRITIONAL REQUIREMENT Hormone in case of plant cell culturing
auxin ,cytokin , gibberellin, absic acid are required
in case of mammalian cell culturing somatotrophin,
hydrocortison ,prolectin etc are required
NUTRITIONAL REQUIREMENT In case of plant cell culturing
Undefined organic supplement required
For example protein hydrosylate Coconut milk Yeast charcoal
GROWTH
Growth refers to a positive change in size, and/or maturation, often over a period of
time.
GROWTH
GROWTH
Characteristic Growth Pattern of Cultured Cells.
Lag phase The first phase of growth after the culture is
seeded is the lag phase, which is a period of slow growth when the cells are adapting to the culture environment and preparing for fast growth.
Exponential phase The lag phase is followed by the log phase
(i.e., “logarithmic” phase) a period where the cells proliferate exponentially and consume the nutrients in the growth medium.
GROWTH Stationary phase
When all the growth medium is spent (i.e., one or more of the nutrients is depleted) or when the cells occupy all of the available substrate, the cells enter the stationary phase where the proliferation is greatly reduced or ceases entirely
CELL METABOLISM Metabolism is sum total of all chemical
process Take place in living cell
Catabolism -breaking down Anabolism –building up
SERUM
the component of blood that is neither a blood cell (serum does not contain RBC or
WBCs) nor a clotting factor it is the blood plasma not including the fibrinogens.
SERUM
SERUM =PLASMA-CLOTTING FACTOR
SERUM
Serum includes all proteins except blood clotting(coagulation)andelectrolyte, antibodies, antigens, hormones, and any exogenous substances (e.g., drugs and microorganisms).
SERUM
Serum can derive from different animal like Horese serum and human serum
Specific serum use for growth of specific cell type
TYPE OF SERUM
Foetal bovine serum(also known as foetal calf serum )
Newborn calf serum Donor calf serum Horse serum Chicken serum Human serum
TOPOINHIBITION
Inhibition of cells surface proliferation as the cell become closely packed on a culture dish they generally suppressed by the term density dependent inhibition is called topoinhibition
The cell would able to receive and transmit signal to the nucleus both positive and negative signal
TOPOINHIBITION
In tissue culture positive signal are provided by substrate (glass or plastic)
Negative signal provided by intercellular contacts topo inhibition
TOPOINHIBITION Serum may reduce topoinhibition by
shielding its receptors Migration of wound would reduce
topoinhibition By reducing intercellular contacts
PH REQULATION Most normal mammalian cell lines grow well at
pH 7.4, and there is very little variability among different cell strains.
However, some transformed cell lines have been shown to grow better at slightly more acidic environments (pH 7.0–7.4), and
some normal fibroblast cell lines prefer slightly more basic environments (pH 7.4–7.7)
Insect cell lines such as Sf9 and Sf21 grow optimally at pH 6.2.
SOURCE SUBSTITUTE thing that takes the place of someone or
something else are called substitute
Iron salt as a replacement for transferrin
Transferin is used in culture as an iron transporter for animal cells however for protein free medium that do not contain component of animal origin ,transferin can be replace by ferric salt