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INTRODUCTION
SIMULATING COLONIC SURVIVAL OF PROBIOTICS IN SINGLE STRAIN PRODUCTS AS COMPARED TO MULTI-STRAIN PRODUCTS
The human gastrointestinal tract contains variety of microbes which may affect the survival of probiotics in a different way. Commercially, multi-strain preparations have been suggested to express an improved functionality over single strain cultures. However, probiotics are often tested as single strains. The aim of this study was to evaluate in an in vitro colon simulation, if the combination of several probiotic strains (HOWARU Restore; Lactobacillus acidophilus NCFM, Lactobacillus paracasei Lpc-37, Bifidobacterium lactis Bl-04 and B. lactis Bi-07) had an influence on each other’s survival as compared to the strains as single preparations.
Figure 1. Illustration of one simulation unit with four sequentially connected glass vessels (V1-V4) mimicking human colon from proximal to distal part. Synthetic ileal fluid as a simulation media, pH controlled, anaerobic conditions at 37 °C.
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S. Forssten*, K. M. Salli*, A.C. Ouwehand*
* DuPont Nutrition & HealthGlobal Health & Nutrition ScienceSokeritehtaantie 2002460 KANTVIK, FINLANDE-mail: [email protected]
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Lactobacillus sppLactobacillus acidophilus
Lactobacillus paracaseiBifidobacterium spp.
B. lactis BL-04B. lactis Bi-07
log1
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Restore NCFM BL-04 Bi-07 NCFM+BL-04 Lpc-37 CTRL
MATERIALS & METHODS
RESULTS
CONCLUSIONS
The in vitro colon simulations of multi-strain and single strain probiotics were performed as previously described (Ref.1, Fig. 1). The control (CTRL) represents the same synthetic ileal medium used for feeding the simulator but without freeze-dried bacteria. DNA was extracted and the quantities of probiotic strains were determined by qPCR.
All strains could be detected after the colon simulations to which they had been fed (Fig 2). There were no substantial differences in levels of the same strain when comparing single and multi-strain products.
Probiotics do not have an antagonistic effect upon each other’s survival when in a multi-strain product compared to a single-strain product. References: 1, Mäkeläinen H, Olli K, Forssten S, Granlund L, Rautonen N, Ouwehand AC. Probiotic lactobacilli in a semi-soft
cheese survive in the simulated human gastrointestinal tract. Int Dairy J. 2009(19):675-83.
Figure 2. Microbial quantities presented as mean ± SEM for the pooled vessels (V1-V4) from three separate colon simulations. X-axis represents the analysed species or strains, while the quantity is expressed as log10 copies per ml. Statistical differences marked for treatment vs con-trol as *p<0.05 (students t-test).
