WELCOME TO MY

PRESENTATION

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A PRESENTATON ON ESCHERICHIA COLI: AN AVIAN

PATHOGEN

PRESENTED BY:ABDULLAH AL MASUDROLL NO.: 0213/11SESSION:2013-14MS IN MICROBIOLOGY

PRESENTED TO:DR. PARITOSH KUMAR BISWAS,DEAN, FACULTY OF FOOD SCIENCES AND TECHNOLOGY, CVASU.

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Kingdom: BacteriaPhylum: ProteobacteriaClass: Gamma ProteobacteriaOrder: EnterobacterialesFamily: EnterobacteriaceaeGenus: EscherichiaSpecies: Escherichia coli (E. coli)

E. coli

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German Pediatrician -studies intestinal flora of infants Bacterium coli commune "Escherichia coli" are named after him.

Dr. Theodore von Escherich(1857-1911)

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E. coli is a gram-negative bacterianon-acid-fastuniform stainingnon-spore-forming bacillus grows both aerobically and anaerobically Rod shaped bacterium. Many strains are motile peritrichous flagella

ESCHERICHIA COLI: BASIC FEATURES

2.0-6.0 μm long and 1.1μm in diameter

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Brief EpidemiologyShiga-toxin producing Escherichia coli (STEC) is a zoonotic pathogen.It causes diarrheal disease in humans and is ofpublic health concern because of its ability to cause outbreaks.Severe disease such as hemorrhagic colitis (HC) or hemolytic-uremic syndrome (HUS) .E. coli O157:H7 is the most prevalent STEC serotype.Causes secondary diseases when host defense cell are being impaired. E. Coli 0157:H7 present enterohemorrhagic pathogen of human which can also be decolonized in chicken.

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Pathotypes of E. coli

1. Enterotoxigenic E. coli( ETEC)

2. Enteropathogenic E. coli (EPEC)

3. Enteroinvasive E. coli (EIEC)

4. Enterohemorrhagic E. coli (EHEC)

5. Enteroaggregative E. coli (EAEC)

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Pathogenecity:Enterotoxigenic E. coli Heat labile toxin

like choleragenAdenyl cyclase activated cyclic AMP secretion water/ions

Heat stable toxin cyclase activated cyclic GMP uptake water/ions

Enteropathogenic E. ColiIntimin an adhesin

Bind to intestinal cell

Causes rearrangement of actin in host cell

Attachment and effacement

Changes in ultrastructure of intestinal cells

Prime cause of diaarrhoea affected with EPEC

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Antigenic Properties1.O (somatic antigen) : 173 serotypes2.K (capsular antigen) : 74 serotypes3.H (flagellar antigen) : 53 serotypes4.F (fimbrial antigen) : 70 serotypes

O1, O2, O35, 078 serotypes are more prevalent in poultry to cause diseases(O1K1 and 02K1 cause severe disease).O antigen polysaccharide phospholipid in nature, can be produced by boiling of culture at 100 c for 1 hour. There is 5 polyvalent antiserum at market, after grouping by polyvalent serum, further serogroup test is done by monovalent antiserum.K antigen generate after heating at 100 c for 1 hour or 121 c for 2.5 hour, are subdivided into L,A,N,B forms. O and K is important.

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MAJOR CLINICAL AND POSTMORTEM FINDINGS

1.Coligranuloma2.Colisepticamia3.Air sac disease4.Avian cellulitis5.Swollen head

syndrome6.Perihepatitis7.Pericardits8.Panopthalmitis9.Yolk sac infection

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PICTURE: COURTESY OF PROF. DR. MASUDUZZAMAN, DPP, CVASU.

pericarditis

perihepatitis

Airsaculitis

DIAGNOSIS-COLIBACILLOSI

S

PICTURE: COURTESY OF PROF. DR. MASUDUZZAMAN, CVASU.

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Extreme perihepatitis

DIAGNOSIS-COLIBACILLOSIS

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Egg in Uterus

Egg peritonitis

DIAGNOSIS-COLIBACILLOSIS

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E coli colony

Diagnosis-Omphalitis

Yolk sac infection

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Sample for diagnosis………….

Depends on where the lesions are located.But liver, heart are the major samples,In case of septicemia and osteomyelitis bone marrow is the sample.

Confirmatory and differential diagnosis

FIG.: E. M.B agar(colonies have a metallic sheen.

FIG: Mac Conkey agar(Large pink color colony of E. coli)

Fig. E. coli on nutrient agar( Smooth, colorless, circular colony

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Continued………….E. Coli can not be normally grown in colony in blood agar because it contains protease.E. Coli is tested positive in lactose fermentation test and consequently E. coli is IMVic test positive which is done after finding of typical growth.Growth in nutrient slant agar taken by scraping and PBS added Heated upto 100 c for 1 hour or 121 c for 2.5 hours For detection of O and K antigenOther detection methodsPCRMLSTPFGE

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Prevention and control:Vaccine 02K1,078K18Inactivated vaccineMultivalent vaccine made from pilli.A live vaccine prepare form naturally occuring non pathogenic piliated strain Bt7 was effacious when used in chickenBest way- Biosecurity and improving hygienic management.Fecal contamination of water, feed must be avoided.Hatching egg must be kept free from fecal contamination.

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Karimi, V. et al (2011) reported that, The significant increase in the incidence of resistance against antibiotics in the E. coli strains isolated from broiler chickens is probably due to the increased use of antibiotics as feed additives for growthpromotion and prevention of diseases, use of inappropriate antibiotics for treatment of diseases, resistance transfer among different between antibiotics used in poultry. Lutful Kabir, S. M(2010) reported that, Avian colibacillosis and salmonellosis are considered to be the major bacterial disease problems in the poultry industry world-wide and these diseases constitute a major public health burden and represent a significant cost in many countries. The economic and public health burden of these diseases have made this topic time demanding.

Matthijs, M.G.R(1973) reported that, More insight in the pathogenesis of a superinfection of E. coli in IBV-infected broilers and in the underlying mechanisms of the increased susceptibility to colibacillosis might lead to new methods in prevention or reduction of colibacillosis.

HAMMOUDI, A. and AGGAD*,H. (2008) reported that, Antibiotic resistances arefrequently encoded by conjugative plasmids or transposons, thus E. coli of avian origin could act as a possible source for the transfer of antibiotic resistancesto other bacterial species including human pathogens.

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THANKS FOR HAVING PATIENCE.