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EnzymEFor better life of humans and animals with new concept enzymes
Feed enzyme•Amylase
•Alkaline Protease
•Cellulase
•Xylanase
•Phytase New generation phytase source from seawater bacteria. High enzyme activity in the PH condition of monogastric animal's digestive tract.
•Lipase GF Calb-1, GF CalB-P and GF CalB-L are highly active preparations
of lipase from Canadian Antartica.
Food•Lactase Lactazme-B is a neutral lactase (EC3.2.1.23) preparation derived from bacillus circulans.
•Chitosanase Chitosan is a natural product derived from chitin, a polysaccharide found in the exoskeleton of shellfish like shrimp or crabs and insects. it inhibits LDL-cholesterol and boosts HDL- cholesterol. In addition, it is known that it helps to control pressure and to prevent constipation. Chitosan oligosaccharide is obtained by an enzymatic hydrolysis of chitosan using chitosanase.
•Chitinase
Industry•Catalase Catalase is en enzyme that presents in the cells of plants, animals and aerobic (oxygen required) bacteria. It promotes the conversion of hydrogen peroxide, a powerful and harmful oxidizing agent, into water and molecular oxygen.
• Dosage can be determined by specific conditions such as feed compositions, processing, cost, etc.• 50~200g/ton of complete feed available.
Expiration date • 12 months from manufacturing date.
Packaging • 20kg paper bag, double layer polyethylene bag.
Recommended Use
Recommended Use
(based on 5,000unit/gram)
Enzyme for feed
Species of livestock Recommended dosage (g/ton of feed) Phytase activity (unit/kg of feed)Laying hens 60 300
Broiler 100 500Pigs 100 500
Others 100 500
Specification Formulation Powder type Granular type Coated type
5.000 IU/g Genophos* 5000P Genophos* 5000G Genophos* 5000C10.000 IU/g Genophos* 10000P Genophos* 10000G Genophos* 10000C20.000 IU/g Genophos* 20000P Genophos* 20000G Genophos* 20000C
AppearanceFine yellowish white powder
Min. 95%(30mesh)Fine yellowish white granule
Min. 95%(25-70mesh)Fine yellowish brown granule
Min. 95%(25-70mesh)
GenoPhos (Phytase)
Enzyme activity : 5,000unit/gram, 10,000unit/gram, 20,000 unit/gram
Innovative Technology - Protein Engineering • High enzyme activity in the PH condition of monogastric animal’s digestive tract.• Strong resistance against digestive proteinase of monogastric animal’s digestive tract.
Feed Adjustable Formulation • Particle size was optimized for maxibility and lowwability in feed manufacturing process.
High Efficacy in Poultry and Pigs • Egg quality (egg weight, Haugh unit, egg shell strength, etc.) in laying hens was increased• Growth performance ( weight gain, FCR, etc.) in poultry and pigs was improved.
Alkaline-ProteaseEnzyme Activity : Alkaline protease 200,000 unit/gramGenoPro-AK is produced by submerged fermentation from Bacillus licheniformis, which can hydrolyze protein into free amino acids. It is widely applied in casein-sodium, chondroitin, leather de-haring, detergent industry and feed industry.
Product description and its storage • Color : yellow to brown • Appearance : powder (85% pass standard sieve 40) Water content: less than 8% • Specific activity : 200,000 unit/g or more (One unit is defined to be the amount of the enzyme that decomposed 1 µmol of the tyrosine for 1 minute at 40°C, pH 10.5.)• Odor : a slight fermentative odor • Packaging : 1kg, 10kg, 25kg and bulk
Reaction condition • Optimal temperature is 40~50°C • Optimal pH is 9~12
Recommended dosage • Dosage 200~400g/ ton Feed
PCT WO 2004085638 (phytase produced from citrobacter braakii)
Opt. Temp. Opt. pH
3050
60
70
80
90
100
100
70 80 90 110Temp.(˚C)
Rela
tive
activ
ity(%
)
3050
60
70
80
90
100
100
7 9 11pH
Rela
tive
activ
ity(%
)
Emulsion Digestion(intestinal lumen)
Absorption(enterocyte) Lymph
DAG
FA
LPA
LPA
AGPAT
MGAT
ACAT
PA
DAG
DGAT
TAG
MPT
ApoB
MAG
CL CE
FA
CL
MAGLipasesPhospholipaseColipase
Mixed micelle
CL
BS
TAG
PL
FA
3050
60
70
80
90
100
100
70 80 90 110Temp.(˚C)
Rela
tive
activ
ity(%
)
3050
60
70
80
90
100
100
7 9 11pH
Rela
tive
activ
ity(%
)
Emulsion Digestion(intestinal lumen)
Absorption(enterocyte) Lymph
DAG
FA
LPA
LPA
AGPAT
MGAT
ACAT
PA
DAG
DGAT
TAG
MPT
ApoB
MAG
CL CE
FA
CL
MAGLipasesPhospholipaseColipase
Mixed micelle
CL
BS
TAG
PL
FA
Enzyme for feed
Geno-Lipa (Lipase)Geno-Lipa is a lipase from candida antartica produced by fermentation of genetically modified Saccharomyces cerevisiae. • Geno-Lipa is a triacylglycerol hydrolize (EC* 3.1.1.3.) and it has 1,3 positional specificity.• Geno-Lipa is strongly bound to the carrier that used for immobilization is microporous ion exchange resin by adsorption.
Activity • Synthetic activity using oleic acid and butanol• Lipase 1,200 ~ 5,000 unit/gram
Opt. Temp. & Opt. pH
Storage and Expiration • Store at dry and cool place• 12 months from manufacturing date
• Before treatment of Lipase to Tallow • 12hours after treatment of Lipase to Tallow
Opt. Temp. : 50ºC Opt. pH : 6.0
Temperature and pH activity of lactase from B.circulans (oNPG method)
3020
40
60
80
100
120
40 50 60 70 80Temp.(˚C)
Rela
tive
activ
ity(%
)
30
20
40
60
80
100
120
4 5 6 7 8 9 10pH
Rela
tive
activ
ity(%
)
3020
40
60
80
100
120
40 50 60 70 80Temp.(˚C)
Rela
tive
activ
ity(%
)
30
20
40
60
80
100
120
4 5 6 7 8 9 10pH
Rela
tive
activ
ity(%
)
Enzyme for food
Specification • Lactazyme-B : 4,000 unit/gram (unit definition: one unit is defined as the amount of enzyme which will liberate 1u mol of per minute from oNGP at 50degree Cel, pH6.0)• Appearance : yellow~light brown, powder• Application : GOS production, Dairy industry
Storage• Store under cool and dry condition
Lactazyme-B (Lactase)Lactazme-B is a neutral lactase (EC 3.2.1.23) preparation derived from Bacillus circulans.The enzyme catalyzes the hydrolysis of lactose and the galactosyl transfer reaction.In the galactosyl transfer reaction, it is advantageous to react at high temperature because of the low solubility of lactose.
Product Properties
Enzyme for food
43210.0 0.0
0.9
15.4
27.9
20.318.9
9.7
6.9
5.0
10.0
15.0
20.0
25.0
30.0
5 6 7 8
Rela
tive
activ
ity(%
)
COS Profile
COS No.
Cook
ing
oil
Storage• Store at dry and cool place ( 2 ~ 8 °C)
CalB-IM (Lipase)GF CalB-Im is a lipase from candida antartica produced by fermentation of genetically modified Saccharomyces cerevisiae.
Product Properties • Product Type GF CalB-IM is a triacylglycerol hydrolize (EC* 3.1.1.3.) and it has 1,3 positional specificity. CalB-IM is strongly bound to the carrier that used for immobilization is microporous ion exchange resin by adsorption. • Activity Synthetic activity using oleic acid and butanol GF CalB-IM™ : 1,200 unit/gram
Opt. Temp. & Opt. pH
GF ChitosanaseChitosan is a natural product derived from chitin, a polysaccharide found in the exoskeleton of shellfish like shrimp or crabs and insects. It inhibits LDL-cholesterol and boosts HDL-cholesterol. In addition, it is known that it helps to control pressure and to prevent constipation.Chitosan oligosaccharide is obtained by an enzymatic hydrolysis of chitosan using chitosanase.Chitosan oligosaccharide is a mixture of oligomers od D-glucosamine and its molecular weight is less than 2.500 dalton.Chitosan oligosaccharide is absorbed very well in the body and its function is very high compared with chitosan.
Product Properties • Bind excess fat and inhibit fat absorption• Lower LDL/boost HDL cholesterol• Anti-cancer, anti bacterial agent• Support immunity-lower blood sugar• Control blood pressure• Prevent constipation• Enhance absorption of calcium• Prevent heart disease
Specification • Origin : Bacillus species• Endo type enzyme, producing mainly 3-6 oligosaccharides• Molecular weight : 46,000 dalton• Optimum temperature : 60 degree celcius• Optimum PH : 7.0• Appearance : Light yellow~white powder• Use : Production of chito-oligosaccharides 1g chitosanase/10kg chitosan, 24hr reatcion at 37degree celcius• Specific activity : 36.000 units/G• Package : 1g,5g,10g,50g,100g,500g,1kg and bulk
temp. pH
300
20
40
60
100
80
120
40 50 60 70 80Temp.(˚C)
Rela
tive
activ
ity(%
)
40
20
40
60
80
100
120
5 6 7 8 9 10pH
Rela
tive
activ
ity(%
)
300
20
40
60
100
80
120
40 50 60 70 80Temp.(˚C)
Rela
tive
activ
ity(%
)
40
20
40
60
80
100
120
5 6 7 8 9 10pH
Rela
tive
activ
ity(%
)
Temperature & Optimum pH
Opt. Temp. Opt. pH
3050
60
70
80
90
100
100
70 80 90 110Temp.(˚C)
Rela
tive
activ
ity(%
)
3050
60
70
80
90
100
100
7 9 11pH
Rela
tive
activ
ity(%
)
3050
60
70
80
90
100
100
70 80 90 110Temp.(˚C)
Rela
tive
activ
ity(%
)
3050
60
70
80
90
100
100
7 9 11pH
Rela
tive
activ
ity(%
)
TLC HPLC
Reaction condition 10,000u chitosanase / 10kg chitosan → 40°C, 24h reaction
Chitosan
Substrate MW20,000 Chitosan
monomer
dimer
trimer
tetramer
pentamerhexmer
M 1 2 3 4
Substrate MW130,000 Chitosan
Chitosanmonomer
dimer
trimer
tetramer
pentamerhexmer
M 1 2 3 4
M : marker 1: 0h 2 : 4h 3 : 16h 4 : 24h
Product Profile
Bleaching Rinsing KatalaseTM & Dyeing
Heat Stability at 70°C pH Stability (after 1 hour)
00
20
40
60
100
80
120
10 20 40 60Time(min)
Rela
tive
activ
ity(%
)
40
20
40
60
80
100
120
5 6 7 8 9 1110pH
Rela
tive
activ
ity(%
)
AB
GF
AB
GF
00
20
40
60
100
80
120
10 20 40 60Time(min)
Rela
tive
activ
ity(%
)
40
20
40
60
80
100
120
5 6 7 8 9 1110pH
Rela
tive
activ
ity(%
)
AB
GF
AB
GF
2H2O2 → 2H2O + O2 H2O2 + RH2 → 2H2O + R
Enzyme for Industry
GFzyme KatalaseCatalase is an enzyme that presents in the cells of plants, animals and aerobic (oxygen requiring) bacteria.It promotes the conversion of hydrogen peroxide, a powerful and harmful oxidizing agent, into water and molecular oxygen.It is widely used for removal of H202 in textile industry and semiconductor. it saves time and energy and is environmental-friendly.
Recommendation of Operational Condition • Stable pH range : pH 4.0 ~ 11.0• Optimum pH : pH 6.0 ~ 8.0• Temperature stability : stable at 70°C for one hour• Optimum temperature : 35 ~ 55°C• Process time : 5 ~ 20 minutes• Dosage : 0.01 ~ 0.05 g/L
Guideline for the Bleach Clean-up • Drain the bleaching liquid• Fill the bath with suitable amount of fresh water• Adjust pH and temperature for using enzyme• Add 0.01~0.05 g/L of GFzyme-KatalaseTM• After 5-20 minutes, continue dyeing procedure in the same bath (Generally no need to change water, but if needed, change water for elimination of impurities)
Comparison of the Process• Rinsing with water
• Using GFzyme-KatalaseTM
Package and Storage • Package unit : 20kg, 220kg drum and 1 ton bulk• Storage : avoid a direct ray of light and keep cool
Stability test for Temperature and pH
Bleaching Rinsing Rinsing Rinsing Rinsing Dyeing
Biogenoci Co., Ltd. in Korea(3F, Dongjin B/D) 45, Nonhyeon-ro 26 Gil, Gangnam-gu, Seoul, Korea, 135-855• Tel : +82-2-323-8570 • Fax : +82-2-323-8574 • e-mail : [email protected]
