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Cover crop mixture diversity, biomass production and weed suppression Angela Tran University of Nebraska-Lincoln

Effect of cover crop mixture species

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69th SWCS International Annual Conference “Making Waves in Conservation: Our Life on Land and Its Impact on Water” July 27-30, 2014 Lombard, IL

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Page 1: Effect of cover crop mixture species

Cover crop mixture

diversity, biomass

production and

weed suppression

Angela TranUniversity of Nebraska-Lincoln

Page 2: Effect of cover crop mixture species

OutlineThe motivationThe question

The methodologyThe outcome

Page 3: Effect of cover crop mixture species

THE MOTIVATIONWhy study diverse cover crop mixtures?

Page 4: Effect of cover crop mixture species

Photo by Tom Stromme of The Bismarck Tribune.

Increasing interest in the use of diverse cover crop mixtures.

Page 5: Effect of cover crop mixture species

Cover crop research mainly on monocultures.

Photo from Practical Farmers of Iowa.

Page 6: Effect of cover crop mixture species

Cover crop mixture research mainly on bi-cultures.

Photo from The Ohio State University Extension.

Page 7: Effect of cover crop mixture species

Little research on diverse, multi-species cover crop mixtures.

Page 8: Effect of cover crop mixture species

0

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0 3 6 9 12

No.

pos

sible

com

bina

tions

No. species

Possible combinations of nspecies.

Page 9: Effect of cover crop mixture species

Missing fundamental information about cover crop mixtures.

How are mixtures different from monocultures?

Why?

How do I design my cover crop mixture?

Page 10: Effect of cover crop mixture species

THE QUESTIONWhat is it that we want to know?

Page 11: Effect of cover crop mixture species

What is the effect of diversity

on performance?

Diversity

Perfo

rman

ce

Page 12: Effect of cover crop mixture species

Diversity

Perfo

rman

ce

?

Page 13: Effect of cover crop mixture species

Diversity

Perfo

rman

ce

?

Page 14: Effect of cover crop mixture species

Diversity

Perfo

rman

ce

?

Page 15: Effect of cover crop mixture species

Defining performance• Biomass production• Weed suppression

Diversity

Perfo

rman

ce

Page 16: Effect of cover crop mixture species

Defining diversity• No. species

• No. functional groups

Diversity

Perfo

rman

ce

Page 17: Effect of cover crop mixture species

Species

Page 18: Effect of cover crop mixture species

Functional groups

Page 19: Effect of cover crop mixture species

THE METHODOLOGYHow did we approach answering our research question?

Page 20: Effect of cover crop mixture species

No. Species Species Functional Groups

1 No Cover 0 02 Barley (Spring) 1 13 Oats (Spring) 1 14 Wheat (Spring) 1 15 Austrian Winter Pea 1 16 Mammoth Red Clover 1 17 Yellow Blossom Sweetclover 1 18 Radish 1 19 Rapeseed 1 1

10 Turnip 1 1

No. Species Species Functional Groups

1 No Cover 0 02 Barley (Spring) 1 13 Oats (Spring) 1 14 Wheat (Spring) 1 15 Austrian Winter Pea 1 16 Mammoth Red Clover 1 17 Yellow Blossom Sweetclover 1 18 Radish 1 19 Rapeseed 1 1

10 Turnip 1 1

Cover crop treatments (#1-10)No. Species Species Functional

Groups1 No Cover 0 02 Barley 1 13 Oats 1 14 Wheat* 1 15 Austrian Winter Pea 1 16 Mammoth Red Clover 1 17 Yellow Blossom Sweetclover 1 18 Radish 1 19 Rapeseed 1 1

10 Turnip 1 1

No. Species Species Functional Groups

1 No Cover 0 02 Barley 1 13 Oats 1 14 Wheat* 1 15 Austrian Winter Pea 1 16 Mammoth Red Clover 1 17 Yellow Blossom Sweetclover 1 18 Radish 1 19 Rapeseed 1 1

10 Turnip 1 1

No. Species Species Functional Groups

1 No Cover 0 02 Barley 1 13 Oats 1 14 Wheat* 1 15 Austrian Winter Pea 1 16 Mammoth Red Clover 1 17 Yellow Blossom Sweetclover 1 18 Radish 1 19 Rapeseed 1 110 Turnip 1 1

*spring variety

Page 21: Effect of cover crop mixture species

No. Species Species Functional Groups

11 Grass Mix (Barley, Oats, Wheat) 3 112 Legume Mix (Peas, Red Clover, Sweetclover) 3 113 Brassica Mix (Radish, Rapeseed, Turnip) 3 114 Grass Mix + Legume Mix 6 215 Grass Mix + Brassica Mix 6 216 Legume Mix + Brassica Mix 6 217 Grass Mix + Legume Mix + Brassica Mix 9 318 Barley, Peas, Radish 3 319 Oats, Red Clover, Rapeseed 3 320 Wheat, Sweetclover, Turnip 3 3

Cover crop treatments (#11-20)No. Species Species Functional

Groups11 Grass Mix (Barley, Oats, Wheat) 3 112 Legume Mix (Peas, Red Clover, Sweetclover) 3 113 Brassica Mix (Radish, Rapeseed, Turnip) 3 114 Grass Mix + Legume Mix 6 215 Grass Mix + Brassica Mix 6 216 Legume Mix + Brassica Mix 6 217 Grass Mix + Legume Mix + Brassica Mix 9 318 Barley, Peas, Radish 3 319 Oats, Red Clover, Rapeseed 3 320 Wheat, Sweetclover, Turnip 3 3

No. Species Species Functional Groups

11 Grass Mix (Barley, Oats, Wheat) 3 112 Legume Mix (Peas, Red Clover, Sweetclover) 3 113 Brassica Mix (Radish, Rapeseed, Turnip) 3 114 Grass Mix + Legume Mix 6 215 Grass Mix + Brassica Mix 6 216 Legume Mix + Brassica Mix 6 217 Grass Mix + Legume Mix + Brassica Mix 9 318 Barley, Peas, Radish 3 319 Oats, Red Clover, Rapeseed 3 320 Wheat, Sweetclover, Turnip 3 3

No. Species Species Functional Groups

11 Grass Mix (Barley, Oats, Wheat) 3 112 Legume Mix (Peas, Red Clover, Sweetclover) 3 113 Brassica Mix (Radish, Rapeseed, Turnip) 3 114 Grass Mix + Legume Mix 6 215 Grass Mix + Brassica Mix 6 216 Legume Mix + Brassica Mix 6 217 Grass Mix + Legume Mix + Brassica Mix 9 318 Barley, Peas, Radish 3 319 Oats, Red Clover, Rapeseed 3 320 Wheat, Sweetclover, Turnip 3 3

No. Species Species Functional Groups

11 Grass Mix (Barley, Oats, Wheat) 3 112 Legume Mix (Peas, Red Clover, Sweetclover) 3 113 Brassica Mix (Radish, Rapeseed, Turnip) 3 114 Grass Mix + Legume Mix 6 215 Grass Mix + Brassica Mix 6 216 Legume Mix + Brassica Mix 6 217 Grass Mix + Legume Mix + Brassica Mix 9 318 Barley, Peas, Radish 3 319 Oats, Red Clover, Rapeseed 3 320 Wheat, Sweetclover, Turnip 3 3

Page 22: Effect of cover crop mixture species

Cover crops broadcasted…

After small grains harvest (3)

Into maturing soybeans (3)

Into maturing corn (2) or after seed corn

harvest (1)

Page 23: Effect of cover crop mixture species

Across nine fields at six farms.

Page 24: Effect of cover crop mixture species

Sampling and measurements

Species specific cover crop and weed biomass sampled before fall frosts . Plant tissue dried to a constant weight at 65°F.

Cover crop and weed biomass sampled before fall frosts.

Page 25: Effect of cover crop mixture species

THE OUTCOMEWhat have we found so far?

Page 26: Effect of cover crop mixture species

Cover crop establishment highly variable across sites.

Page 27: Effect of cover crop mixture species

Only one site with substantialcover crop growth.

Hooper, NE (harvested wheat field)Cover crops seeded 8/31/13.

Plant biomass sampled 10/31/13.

Page 28: Effect of cover crop mixture species

Biomass productionData has many possible narratives.

An ecological narrative• Pre-existing framework

• ↑ diversity, ↑ productivity

An agronomic narrative• More relevant to agricultural management?

• < diverse systems can be just as productive as > diverse systems

Page 29: Effect of cover crop mixture species

0

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0

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Barley Oats WheatPea Red Clover SweetcloverRadish Rapeseed Turnip

Error bars (±SEM).

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

Treatment No.

Page 30: Effect of cover crop mixture species

An ecological narrative

Tilman, D. 2001. Functional Diversity. p. 109-120 in S. A. Levin (ed.) Encyclopedia of biodiversity. Academic Press: San Diego, CA.

Tilman, D., J. Knops, D. Wedin, P. Reich, M. Ritchie, E. Siemann. 1997. The Influence of Functional Diversity and Composition on Ecosystem Processes. Science 277: 1300-1302.

Page 31: Effect of cover crop mixture species

0

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0 1 2 3 4 50

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0 3 6 9

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

No. functional groupsNo. species

Data fit to rectangular hyperbola (Y = I*X/[1+(I/A)*X]). Error bars (±SEM).

Page 32: Effect of cover crop mixture species

An agronomic narrative

Less concerned with averages.

More concerned with instances.

Are there instances where less diverse systems produce more than

more diverse systems?

Page 33: Effect of cover crop mixture species

0

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0 1 2 3 4 50

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0 3 6 9

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

No. functional groupsNo. species

Species diversity confounded with functional diversity.

Page 34: Effect of cover crop mixture species

0

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Grasses Legumes Brassicas

1 species 3 species

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

Functional group

Error bars (±SEM); p-value <0.10.

NS

NS

NS

Page 35: Effect of cover crop mixture species

Monocultures and 3-way mixes within the same functional

group performed the same.

On average.

Not in every instance.

Page 36: Effect of cover crop mixture species

Error bars (±SEM); p-value <0.01.

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

-----------NS-----------

Functional groupGrasses Legumes Brassicas

A

B

B

B

A

BB B

0

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0

100

200

300

400

500Barley Oats WheatPea Red Clover SweetcloverRadish Rapeseed Turnip

Page 37: Effect of cover crop mixture species

With regard to species diversity alone, monocultures can

outperform mixtures.

Page 38: Effect of cover crop mixture species

What about with regard to functional diversity?

Page 39: Effect of cover crop mixture species

B

A

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1 3

No. species = 3; p-value <0.01.

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

No. functional groups

Page 40: Effect of cover crop mixture species

Increasing functional diversity increased biomass production.

On average.

Not in every instance.

Page 41: Effect of cover crop mixture species

1 3

No. functional groups

-------------------NS-------------------

No. species = 3; p-value <0.01.

B

C

A

0

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0

100

200

300

400

500Barley Oats WheatPea Red Clover SweetcloverRadish Rapeseed Turnip

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

Page 42: Effect of cover crop mixture species

Biomass production

Possible narratives

Ecological—↑ diversity, ↑ productivityThe narrative of averages

Agronomic— < diverse systems can be just as productive as > diverse systems

The narrative of instances

Page 43: Effect of cover crop mixture species

Weed SuppressionData range limitations prevented

evaluation of the relationship between mixture diversity and weed suppression

Page 44: Effect of cover crop mixture species

0

10

20

30

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0 100 200 300 400

Wee

d b

iom

ass

(g/m

2 )

Cover crop biomass (g/m2)

No cover

Legume only covers

All other covers

Page 45: Effect of cover crop mixture species

Looking ForwardBeyond biomassNutrient cycling

Faunal biodiversity

Adding functional groupsWarm season grasses, legumes,

broadleaves

Page 46: Effect of cover crop mixture species

QUESTIONS/COMMENTS

Cover crop mixture diversity, biomass production and weed suppression

Page 47: Effect of cover crop mixture species

EXTRA SLIDES

Cover crop mixture diversity, biomass production and weed suppression

Page 48: Effect of cover crop mixture species

Biomass production

On the mechanisms behind diversity-productivity relationship

Suggested mechanisms include the idea that one species enhances the success of another.

For this project, however, mechanism may be a simple artifact of the relationship between

seeding rate and biomass.

Page 49: Effect of cover crop mixture species

0

1/3

2/3

1

Full2/31/3

Biom

ass

pote

ntia

l

Seeding rate

Page 50: Effect of cover crop mixture species

Radish as an exampleRecommended broadcast rate*: 8-14 lb/A

Selected rate for this project: 15 lb/AWith ≈ 25,000 seeds/lb, that is seeding ≈ 9 seeds/ft2.

*Clark, A. (ed.) 2007. Managing cover crops profitably. 3rd ed. SARE Program, College Park, MD.

Page 51: Effect of cover crop mixture species

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8 18

Barley Pea Radish

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

Treatment No.

Could more diverse systems be more economical ways to get more biomass?

Page 52: Effect of cover crop mixture species

Seeding datesLocation Field Type Seeding Date

Keene, NE Wheat stubble 7/19/13

Marquette, NE Barley stubble 8/10/13

Hooper, NE Wheat stubble (disked) 8/31/13

Mead, NE Soybean (R5) 9/10/13

Hooper, NE Soybean (R7) 9/12/13

Marquette, NE Soybean (R6) 9/14/13

Hooper, NE Corn 9/12/13

Waterloo, NE Corn stubble (disked) 9/19/13

Bladen, NE Corn 9/20/13

Page 53: Effect of cover crop mixture species

Seeding ratesSpecies

Full broadcast rate (lb/A)

Suggested * Selected

Barley 80-125 150

Oats 110-140 150

Wheat 60-150 150

Austrian winter pea 90-100 100

Red clover 10-12 15

Yellow blossom sweetclover 10-20 15

Radish 10-20 15

Rapeseed 8-14 15

Turnip 10-12** 15*Clark, A. (ed.) 2007. Managing cover crops profitably. 3rd ed. SARE Program, College Park, MD.

**Björkman, T. 2009. Forage Turnips and Rape. Available at http://www.hort.cornell.edu/bjorkman/lab/covercrops/turnip.php (verified 3 Mar . 2013). Cornell University, Cornell, NY.

Page 54: Effect of cover crop mixture species

Liz Morrison, Corn and Soybean Digest (Jan. 1, 2011).

John Dietz, Hay & Forage Grower (Aug. 1, 2008).

Graze (May 1, 2013).

Cover Crop Cocktail Research Team, Penn State College of Agricultural Sciences (2013).This image cannot currently be displayed.

Andrew McGuire, Washington State University (April 17, 2013).

This image cannot currently be displayed.

This image cannot currently be displayed.

John Michaelson, Tennessee News Service (Jan. 20, 2014).

Page 55: Effect of cover crop mixture species

C

BB

AB

A

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0 1 3 6 9

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

No. species

Error bars (±SEM); p-value <0.10.

Page 56: Effect of cover crop mixture species

C

B

AB

A

0

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3000

0

100

200

300

400

0 1 2 3

Error bars (±SEM); p-value <0.01.

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

No. functional groups

Page 57: Effect of cover crop mixture species

D

C

B

A

0

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3000

0

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200

300

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0 1 2 3

Error bars (±SEM); p-value <0.01 All three species included for addition of each functional group.

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

No. functional groups

Page 58: Effect of cover crop mixture species

0

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0

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2 5 8 18

Barley Pea Radish

Treatment No.

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

Page 59: Effect of cover crop mixture species

0

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0

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2 5 8 AVG(2,5,8) 18

Barley Pea Radish

Treatment No.

Cov

er c

rop

biom

ass (

g/m

2 ) Cover crop biom

ass (lb/A)

Page 60: Effect of cover crop mixture species

0

8

16

24

0

20

40

60

0 20 40 60 80 100 120Depth (in)

Dept

h (c

m)

Soil Nitrate (ppm)

No Cover9-Way Cocktail

p-value < 0.10 (*), <0.01 (**)

*

*

**

**

Page 61: Effect of cover crop mixture species

0

8

16

24

0

20

40

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0 20 40 60 80 100 120Depth (in)

Dept

h (c

m)

Soil Nitrate (ppm)

No CoverGrass MixLegume MixBrassica Mix9-Way Cocktail

p-value < 0.10 (*), <0.01 (**)

*

**

**

**

Page 62: Effect of cover crop mixture species

0

8

16

24

0

20

40

60

0 4 8 12 16 20Depth (in)

Dept

h (c

m)

Soil Sulfate (ppm)

No CoverGrass MixLegume MixBrassica Mix9-Way Cocktail

p-value < 0.10 (*), <0.01 (**)

*

**

*

*

Page 63: Effect of cover crop mixture species

0

8

16

24

0

20

40

60

0 4 8 12 16 20Depth (in)

Dept

h (c

m)

Soil Ammonium (ppm)

No CoverGrass MixLegume MixBrassica Mix9-Way Cocktail

NS

p-value < 0.10 (*)

*

NS

*

Page 64: Effect of cover crop mixture species

0

8

16

24

0

20

40

60

0 40 80 120 160 200 240Depth (in)

Dept

h (c

m)

Soil Phosphorus (ppm)

No CoverGrass MixLegume MixBrassica Mix9-Way Cocktail

p-value < 0.10 (*), <0.01 (**)

**

NS

NS

NS

Page 65: Effect of cover crop mixture species

0

8

16

24

0

20

40

60

0 200 400 600 800 1000Depth (in)

Dept

h (c

m)

Soil Potassium (ppm)

No CoverGrass MixLegume MixBrassica Mix9-Way Cocktail

p-value < 0.10 (*), <0.01 (**)

*

NS

*

*

Page 66: Effect of cover crop mixture species

This image cannot currently be displayed.

It makes little sense if you know what you are doing.

Diversification is protection against ignorance.

Photo by Ben Baker of Redux.