1. Three-Dimensional Scaffold Direct Writer For Fabricating
Fiber-Reinforced Materials Rohan Rath Industrial & Systems
Engineering
2. Unit Name Optional Presentation Title Objective Design an
automated machine for fabricating fiber- reinforced materials: 3D
Scaffold Direct Writer (SDW) Establish a manufacturing process for
fabricating continuous-fiber tissue engineered scaffolds Student
Technical Paper Competition May 31, 2015
3. Unit Name Optional Presentation Title Background Student
Technical Paper Competition May 31, 2015 (d) Synthetic meniscus [4]
(a) Synthetic ear [15] (c) Electrospun skin [17] (b) Printed heart
[16] Customizable biomaterials are feasible raw material Greater
customization through flexible manufacturing is necessary
Engineered tissue + flexible manufacturing Bio-manufacturing
4. Unit Name Optional Presentation TitleStudent Technical Paper
Competition May 31, 2015 Meniscal injuries: 1.4 million surgical
interventions annually (US & EU) Tissue supply chain: limited
by donation (allograft) Menisci are not self-healing
Background
5. Unit Name Optional Presentation TitleStudent Technical Paper
Competition May 31, 2015 Background Autograft, collagen meniscus
implant (CMI) CMI + filament matrix (a) = RWJ implant (c) RWJ
implant + SDW = Meniscus Bio-manufacturing (a) Internal filament
matrix [4] (c) Freeze-dried final product [4](b) Manual
substrate
6. Unit Name Optional Presentation TitleStudent Technical Paper
Competition May 31, 2015 MarkForged MarkOne [1] Microfluidic Direct
Writer [3] Current Mass Customization Technology
7. Unit Name Optional Presentation TitleStudent Technical Paper
Competition May 31, 2015 (b) Prototype collagen tool (a) Writing
tool (c) 80/20 frame (f) XY motion platform (e) Writing
substrate(d) Z axis ball screw SDW System SDW = Writing Tool +
Writing Substrate + XY Platform
8. Unit Name Optional Presentation Title (g) Spool pinch (a)
Spring housing (e) Spring (d) Fiber spool (between frames) (b)
Laser-cut acrylic frames (f) Syringe needle (c) Swivel spring
anchors Student Technical Paper Competition May 31, 2015 SDW
Subsystem: Writing Tool Replaces the human hand Pinch allows
variable settings
9. Unit Name Optional Presentation TitleStudent Technical Paper
Competition May 31, 2015 Re-usable Vertically oriented pins One pin
diameter Three discrete layouts Pin pre-configured scaffold support
structure SDW Subsystem: Writing Substrate (a) ~3 [mm] pin (c)
Removable, pin-inserted substrate (d) XY mount plate (b)
Reinforcing filament
10. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 SDW System
11. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 (a) Present pin top vertex: SDW tool
starting position Tool Path Terminology
12. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 (b) Ending pin top vertex: SDW tool
ending position Tool Path Terminology (a) Present pin top vertex:
SDW tool starting position
13. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 (c) Traces of tool positions Tool
Path Terminology (a) Present pin top vertex: SDW tool starting
position (b) Ending pin top vertex: SDW tool ending position
14. Unit Name Optional Presentation Title (d) Intermediate
filament positions (c) Traces of tool positions Student Technical
Paper Competition May 31, 2015 Tool Path Terminology (a) Present
pin top vertex: SDW tool starting position (b) Ending pin top
vertex: SDW tool ending position
15. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 (d) Intermediate filament positions
(c) Traces of tool positions (e) Ending pin bottom vertex Tool Path
Terminology (a) Present pin top vertex: SDW tool starting position
(b) Ending pin top vertex: SDW tool ending position
16. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 (d) Intermediate filament positions
(f) Permanent filament trajectory: (c) Traces of tool positions (e)
Ending pin bottom vertex Tool Path Terminology (a) Present pin top
vertex: SDW tool starting position (b) Ending pin top vertex: SDW
tool ending position
17. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 (b) Layer two subgraph: (a) Layer
one subgraph: (c) Layer three subgraph: Stacked 3D subscaffold: the
filament matrix between any pair of pins has at most three distinct
layers Tool Path Terminology
18. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Tool Path Terminology
19. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Red Partial = subscaffold of
subgraphs - Subgraph layers 1 = sets of permanent filament
trajectories Stacking subgraphs defines a 3D subscaffold, , (1, +
2) Subscaffold sequence defines the 3D scaffold S; explicitly: 6 =
{ ,1, ,1 , ( ,1, ,3),( ,3, ,4), ( ,3, ,3), ,3, ,3 , ( ,3, ,3)} 12 =
1 + 2 + + + + 14 = {1, , 10, , 4, 1} Tool Path Terminology
20. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Partial = subscaffold of subgraphs -
Subgraph layers 1 = sets of permanent filament trajectories
Stacking subgraphs defines a 3D subscaffold, , (1, + 2) Subscaffold
sequence defines the 3D scaffold S; explicitly: 6 = { ,1, ,1 , (
,1, ,3),( ,3, ,4), ( ,3, ,3), ,3, ,3 , ( ,3, ,3)} 12 = 1 + 2 + + +
+ 14 = {1, , 10, , 4, 1} Tool Path Terminology Red Orange
21. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Partial = subscaffold of subgraphs -
Subgraph layers 1 = sets of permanent filament trajectories
Stacking subgraphs defines a 3D subscaffold, , (1, + 2) Subscaffold
sequence defines the 3D scaffold S; explicitly: 6 = { ,1, ,1 , (
,1, ,3),( ,3, ,4), ( ,3, ,3), ,3, ,3 , ( ,3, ,3)} 12 = 1 + 2 + + +
+ 14 = {1, , 10, , 4, 1} Tool Path Terminology Red Orange
Purple
22. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Partial = subscaffold of subgraphs -
Subgraph layers 1 = sets of permanent filament trajectories
Stacking subgraphs defines a 3D subscaffold, , (1, + 2) Subscaffold
sequence defines the 3D scaffold S; explicitly: 6 = { ,1, ,1 , (
,1, ,3),( ,3, ,4), ( ,3, ,3), ,3, ,3 , ( ,3, ,3)} 12 = 1 + 2 + + +
+ 14 = {1, , 10, , 4, 1} Tool Path Terminology Red Orange Purple
Green
23. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Partial = subscaffold of subgraphs -
Subgraph layers 1 = sets of permanent filament trajectories
Stacking subgraphs defines a 3D subscaffold, , (1, + 2) Subscaffold
sequence defines the 3D scaffold S; explicitly: 6 = { ,1, ,1 , (
,1, ,3),( ,3, ,4), ( ,3, ,3), ,3, ,3 , ( ,3, ,3)} 12 = 1 + 2 + + +
+ 14 = {1, , 10, , 4, 1} Tool Path Terminology Red Orange Purple
Green Blue
24. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Partial = subscaffold of subgraphs -
Subgraph layers 1 = sets of permanent filament trajectories
Stacking subgraphs defines a 3D subscaffold, , (1, + 2) Subscaffold
sequence defines the 3D scaffold S; explicitly: 6 = { ,1, ,1 , (
,1, ,3),( ,3, ,4), ( ,3, ,3), ,3, ,3 , ( ,3, ,3)} 12 = 1 + 2 + + +
+ 14 = {1, , 10, , 4, 1} Tool Path Terminology Red Orange Purple
Green Blue Black
25. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Tool Path Terminology 3D
subscaffolds: unique constant-tension filament matrices that define
implant geometry
http://patentimages.storage.googleapis.com/US20140031933A1/US20140031933A1-20140130-D00009.png
: 1- 3 : 1- 6 : 1- 8 : 1- 10 : 1- 12 : 1- 14
26. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 System Complexity: 3D Stacking
27. Unit Name Optional Presentation Title Manual versus SDW
Fabrication Student Technical Paper Competition May 31, 2015
Non-stop, no collision direct writing Reduces variability and human
error Creates acceptable FDA process On-line optical quality
control feasible (a) Manual product [4] (b) SDW product
28. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Business Process: Current Random
donor death Disease screening Tissue removal surgery Bio-
preserving transport Storage (FDA regulated) Bio- preserving
delivery Transplant/arthroscopy surgery ($11900 US avg.) JIT organ
matching (limited supply) Extensive patient screening &
assessment
29. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Business Process: Proposed In-house,
FDA approved, custom implant manufacture ($1k - $2k) Implant
Surgery ($3k - $5k) Biomaterial batch production Extensive patient
screening & assessment
30. Unit Name Optional Presentation TitleStudent Technical
Paper Competition May 31, 2015 Impact on Business Process Relies on
organ donation Two surgeries Live tissue possibility of disease
transmission Complex transport logistics Size-matching complexities
Unbound biomaterial supply One surgery Synthetic tissue no
possibility of disease transmission In-house inventory no
transportation needed Size-customizable implants Current
Proposed
31. Unit Name Optional Presentation Title Conclusion Student
Technical Paper Competition May 31, 2015 Create an efficient and
controllable manufacturing process for fiber-reinforced scaffolds
Utilize IE knowledge to build a machine, as a part of a design
course, within budget, time and resource limitations Introduce new
mass customization technology in a focused area of implant tissue
engineering
32. Unit Name Optional Presentation Title Project members : J.
Levy, K. MacKinnon, D. Vas Advising professors: E. Elsayed, K. Li
RWJ Orthopedics: M. Dunn, C. Gatt, J. Patel Rutgers Makerspace
laboratory: R. Anderson Student technical paper competition
sponsor: John Deere Funding Sources: Rutgers ISE: M. Jafari Rutgers
SOE: I. Rosen IIE South Jersey Delaware Valley: J. McGowan ISERC
stipend: IIE National NSF student travel grant: S. Cetinkaya, J.
Ryan Acknowledgements Student Technical Paper Competition May 31,
2015
33. Unit Name Optional Presentation Title Reference Student
Technical Paper Competition May 31, 2015 [1] MarkOne". MarkForged.
https://markforged.com/mark-one/ [2] Moutos, F. T., Freed, L. E.,
Guilak, F. (2007). A biomimetic three dimensional woven com- posite
scaold for functional tissue engineering of cartilage. Nature
Materials, 6(2), 162-167. [3] Ghorbanian, S., Qasaimeh M. A.,
Akbar, M., Tamayol, A., Juncker, D. (2014). Microfluidic Direct
Writer with Integrated Declogging Mechanism for Fabricating
Cell-Laden Hydrogel Constructs. Biomedical Microdevices, 16(3),
387-395. [4] Balint, E., Gatt C., J., Dunn M. G..(2011). Design and
mechanical evaluation of a novel fiber- reinforced scaffold for
meniscus replacement. Orthopaedic Research Laboratories. [5]
Shybut, T., Strauss, E. J. (2011).Surgical Management of Meniscal
Tears. Bulletin of the NYU Hospital for Joint Diseases, 69(1), 56.
[6] Newman, A. P., Daniels, A. U., Burks R. T. (1993).Principles
and Decision Making in Menis- cal Surgery. Arthroscopy Association
of North America. The Journal of Arthroscopic and Related Surgery,
9(1), 33-51.
34. Unit Name Optional Presentation Title Reference Student
Technical Paper Competition May 31, 2015 [7] Tovar, N., Bourke, S.,
Jae, M., Murthy S. N., Kohn, J., Gatt, C., Dunn, M. G. (2010). A
Comparison of Degradable Synthetic Polymer Fibers for Anterior
Cruciate Ligament Reconstruction. J. Biomedical Material Research
Association, 93(2), 738-747. doi: [8] Tamayol, A., Akbari, M.,
Annabi, N., Paul, A., Khademhosseini A., Juncker, D. (2013) Fiber-
Based Tissue Engineering: Progress, Challenges, and
Opportunities.Biotechnol Adv., 31(5), 669-687. doi:
10.1016/j.biotechadv.2012.11.007. [9] Hong, Y.,Gong, Y., Gao, C.,
Shen, J. (2006). Collagen-coated polylactide microcarriers/chitosan
hydrogel composite: Injectable scaffold for cartilage regeneration.
Wiley InterScience (www.interscience.wiley.com). doi:
10.1002/jbm.a.31603. [10] Robert Jan Peter van der Wal.(2009)
Long-term Clinical Outcome of Open Meniscal Al- lograft
Transplantation. American Journal of Sports Medicine. November
2009. 37(11). Pp. 2134-2139. [11] Sun, W. (2013).Bio-3D
Printing.National Science Foundation Workshop on Frontiers of
Additive Manufacturing Research and Education.
35. Unit Name Optional Presentation Title Reference Student
Technical Paper Competition May 31, 2015 [12] Akbari, M., Tamayol,
A., Laforte, V., Annabi, N., Khademhosseini A., Juncker D. (2013)
Continuous Manufacture of Robust Living Fibers That Withstand
Common Textile Processing for Tissue Engineering Applications.
International Conference on Miniaturized Systems for Chemistry and
Life Sciences. [13] Richards, D. J., Tan, Y., Jia, J., Yao, H., Mei
Y. (2013).3D Printing for Tissue Engineering. Israel Journal of
Chemistry, 53, 805-814. [14] Nielsen AB, Yde J. (1991).
Epidemiology of acute knee injuries: a prospective hospital
investigation. J Trauma, 31(12), 1644-1648. [15]
http://dailynewsdig.com/wp-content/uploads/2013/07/bionic-ear.jpg
[16]
https://www.asme.org/getmedia/f8c45f03-a1f7-4e94-8c7d-8772ff0b8c69/Creating-Valve-
Tissue-Using-3D-Bioprinting_02.jpg.aspx?width=340 [17]
https://s-media-cache-
k0.pinimg.com/236x/08/d8/35/08d835697d6ed5fe1cbd9c03972bb74f.jpg
36. Unit Name Optional Presentation Title Questions? Student
Technical Paper Competition May 31, 2015