Next Generation Sequencing

Helicos – True Single Molecule Sequencing (tSMS)

Library Preparation

• Shearing of DNA

• Sonication

• Nebulization

• Enzyme digestion

• Denature dsDNA

• Tailing

• Blocking

Sample Loading and Flow Cell

Preparation

• Sample Fragments Loaded

• Nucleotides are added sequentially

Sequencing by Synthesis

Work Cited

• Ambry Genetics. Making Sense of NextGent Sequencing. Kelly Gonzalez, MS,

CGC, and Senior Manager of Clinical Genomics. Available at

http://www.ambrygen.com/sites/default/files/pdfs/NERRG_4-10-

12_Making_Sense_of_NetGen_Sequencing_KG(3).pdf. Access verified May

21, 2014.

• Omixon. Allen Van Deynze, 2010; Solanaceae Coordinated Agricultural Project.

Next Generation Sequencing. Available at http://www.omixon.com/the-basics-

of-next-generation-sequencing/. Access verified May 21, 2014.

• http://en.wikipedia.org/wiki/Helicos_single_molecule_fluorescent_sequencing

Access verified July 30, 2014.

• Seqll. Howe it Works. 2014. http://seqll.com/the-sample-process/ Access

verified August 1, 2014.

Recommended Video

Helicos True Single Molecule Sequencing

https://www.youtube.com/watch?v=_-

MK3MGfPvY

Next Generation Sequencing

Single Molecule Real Time Method