Comprehensive Marine Biodiversity Survey

Volunteer Dialogue Session III31 August 2012

Part I of CMBS60 Mudflat surveys done since beginning of

projectsLab sorting sessions held in TMSI (NUS

campus) after each surveys

No. of specimens collected 10368 (More sorting

n counting to go)

No. of specimens identified to species 4012% of specimens identified to species 38.7%No. of species collected and identified 315

Mudflat survey (2010 – 2012)

Localities surveyed (With Volunteers)

LATEST SURVEYS IN RED, PREVIOUS SURVEYS IN BLUEMF1, 34 Sungei Buloh MF2, 12 Kranji (Beside nature trail)MF3,36, 46 Sungei CinaMF4 Pulau UbinMF5,23 Sungei PandanMF6, 37,43 Sungei MandaiMF7, 30 Lim Chu KangMF16 St John Island (Beside jetty)MF38, 41 Pulau Ubin (Kampong Melayu)MF39, 40 Semakau Landfill MF45 SeletarMF50 Sungei Api ApiMF52 Changi CreekMF58 Pasir Ris (Between Sungei Api Api and Sungei Tampines) MF67 Sungei Loyang

Localities surveyed (TMSI team)

LATEST SURVEYS IN RED, PREVIOUS SURVEYS IN BLUEMF8 West of Woodlands WaterfrontMF9 Pulau Ubin OBS sitesMF10, 53 St John Island MF11 Lazarus IslandMF13 Pulau Ubin (Kampong Melayu)MF14 Pulau Ubin (Mamam Beach)MF15 Pulau Hantu (Big Lagoon)MF22,28 Pasir Ris (Between Sungei Api Api and Sungei Tampines)MF24 Tuas (Tuas West Drive)MF25 Changi CreekMF26, 62 Western Catchment (Off Pulau Pergam)MF27, 30 Lim Chu Kang (Beside Girl Guide Camp, Old waterfront house) MF29 Seletar (North shore of Yishun Ave 1)MF31, 32 Semakau Landfill (Stonefish Bay, South replanted mangrove)MF33 Punggol (Along track 22)MF35 Sungei Jurong (Along Jalan Papan)MF 42 Pulau KetamMF 44, 57 Pulau Tekong (north)MF48 SerimbunMF 49 Sungei SimpangMF51 Coney IslandMF55, 59 Sungei LoyangMF60 Pulau Tekong (Sungei Unum)MF61, 64 Pulau PawaiMF63 Pulau SenangMF65 Pulau SudongMF66 Pulau Sarimbun

Mudflat survey Sampling methodology

Recce / TMSI team only Random sampling to assess general biodiversity

With volunteers Belt transects (50 m) as standard protocol for qualitative biodiversity information

Quantitative data for specific sites and targeted biota to be carried out in the future

Material needs to be preserved for:accurate species identificationvoucher (reference) material in RMBR

Two major components of faunal diversity: Infauna Epifauna

Infaunal sampling using simple manual excavation, 1 mm/2 mm sieves, yabby pumps

Epifaunal sampling using seines/push nets

Sampling for meiofauna (small benthic invertebrates that are < 1 mm in maximum dimension) using fine sieves

Mudflat survey Sampling methodology

Seabed faunal survey (2012–2014)Part II of CMBSSampling of subtidal benthic fauna within Singapore Port

limitsVolunteers are needed both onboard vessel and in the

laboratory for sorting and identification10 nautical grids have been covered done since beginning of

surveysLab sorting sessions are held in TMSI (St John’s Island and

NUS campus) after surveys

No. of specimens collected 1912 (More sorting n counting to go)

No. of specimens identified to species 127

% of specimens identified to species 6.6%

No. of species collected and identified 54

Localities surveyed

NAUTICAL GRIDS SURVEYED3923 off P. Pergam (south)3924 off P. Pergam (north)4517 off Republic of Singapore Yacht Club4516 off Pasir Panjang Ferry Terminal4513, 4613 betw P. Bukom and P. Semakau4612, 4511 south of P. Semakau4712 Betw P. Semakau, P. Sebarok, and P.

Jong5013 betw St John’s Island, Sister’s

Islands, and P. Tekukor

Rectangular dredge for benthic fauna

Otter trawl for pelagic fauna

Grabs and bottom seines

Seabed survey Sampling methodology

Reef survey (2012–2014)Part III of CMBSSampling of intertidal and subtidal reefs in

Singapore Strait using SCUBA, supplemented by reef walks during low tide

Volunteers are needed both in and out of water for sampling invertebrates and fish

Preliminary results from the March 2012 Ubin Mini Workshop

What lives in the Johor Straits?

Amphipods - 16 genera, 20 speciesFish – 27 genera, 31 speciesPolychaetes – 22 genera, 25 speciesHermit crabs – 4 genera, 15 speciesTrue crabs – 31 genera, 34 species Bivalves - >3o species and still countingGastropods - >30 species and still counting

Identified fauna

Amphipods

Stenothoe gallensis Walker, 1904

Caprella equilibra Say, 1818

Bemlos sp. Walker, 1904

Polychaetes

Diopatra claparedii Grube, 1878New species record in Singapore! Loimia cf. medusa

Spaghetti worm

2 new family records of Singapore (Orbiniidae, Sigalionidae).17 new species records and still counting!

Fishes

Batrachomoeus trispinosus

Ichthyocampus carce

Omobranchus punctatus

Other taxa

Calyptraea extinctorium

Philyra olivaceaSyphonota geographica

Undetermined sea cucumber

Lingula anatina