WE

L

OM

C

E

My Presentation

St ID. 20121209042

Rupom Mitra

Department o

f Pharm

acy,

Bongobondhu Sheikh Mujib

ur Rahman Scie

nce & Technology Universit

y,

Gopalgonj, Bangladesh.

A presentation on

Extraction of DNA from Plant

Presentation Outline• DNA?• Structure of DNA• Method of DNA isolation• Maxwell® 16 Plant DNA Kit Method• Spin column Method• CTAB Method• Reagent Used In CTAB Method• Procedure for CTAB method• Why DNA Extraction?• Storage of isolated DNA• Use of the DNA

DNA?Something to say about DNA

Cell Nucleus is the empire DNA

Structure of DNA

Adenine-Thymine Base Pair

Guanine-Cytosine Base Pair

1. Maxwell® 16 Plant DNA Kit Method

2. Spin column Method3. CTAB Method

Method of DNA isolation

Ridge end

Label end

ContentsLysis BufferMagneSil PMPsWash BufferWash BufferWash BufferWash BufferWash Buffer

PlungerReagent Cartridge

Maxwell® 16 Plant DNA Kit Method

An easy method efficient Automated purification of

genomic DNA from plant tissue samples.

Spin column Method It is a solid phase extraction methodQuickly purify nucleic acids. Nucleic acid will bind to the solid phase of silica under certain conditions.

CTAB MethodDetergent+

Lipid & Proteins Detergent

DNA

Cell Membrane

CTAB Stands for cetyl trimethyl ammonium bromide

Reagent Used

oCTAB oChloroform oIsoamyl alchohol oIsopropyl alcohol oRNAse oQiagen

oAmbion o Proteinase K oNaCl oMercaptoethanol oAmmonium acetate

Procedure for CTAB method

Sample extract

CATB Centrifuge Carbohydrates, proteins, etc.

Nucleic acid-CTAB complex

DNA

Precipitated Nucleic acid-CTAB complex

Why DNA Extraction?• Genetic Testing

Body Identification

• Analysis of Forensic Evidence

There are four temperature based strategies for long term DNA conservation. These are storage at: -20° C -80° C -196° C Dried, at room temperature Store DNA in dry state, reduces molecular mobility and inhibits the

depurination, depyrimidination, deamination and hydrolyticThe DNA immobilized on FDA cards is amenable to PCR after 17

years storage. DNA in aqueous phase is stored under slightly basic conditions.At -196° C, DNA is maintained in a vitreous state

General consideration for DNA storage:

Understanding Individuality of PlantsUsing genetic information of DNA for plant modification Use in forensic analysisUse to solve historical puzzle