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Group Members
•Adan Fatima Javed
•Sehrish Shoukat
•Zunaira Khalil
•Basharat Khan
•Syed Haider Ali
•Salimullah Khan Ayubi
PROJECT INSTRUCTOR: MISS ARISH NASEEM
Isolation and Identification of
Pesticide Degrading Bacteria in
Microbiology LAB
Pesticides Pesticides are substances meant for
preventing, destroying or mitigating any pest.
75%
14%
9%
2%Insecticides
Herbicides
Fungicides
Acricides
Types of pesticides/Classes breakup
Why Pesticides are used?
• Population pressure
• Food security
• Higher yield
• Province wise distribution
KPK 2%Sindh 8%Punjab 90%
Pesticide Use in Pakistan
Direct impact on humans
• Adversely impact on Central Nervous System (CNS)
• Toxic
Toxicity potential of Pesticides
Fate of Pesticide in Environment
Profenofos
Selected Pesticide-Profenofos
• Widely used in Pakistan
• Hazardous to health
• Among top ten used pesticides in Pakistan
– Used on cotton
– Commonly found as groundwater contaminants
– More effective
Reasons
• Microorganisms
• Use compound as carbon and energy source
Biodegradation
Methodology
Enrichment Acclimatization
Serial DilutionsSpreadingIncubation
Plate Count Isolation Characterization
Sampling
Methodology Steps
Media
Mineral Salt Media (MSM)
Use Distilled Water
For 100 ml of media
(0.15g/100 ml)
Autoclave
•Media
•Glass wares
e.g petri plates etc….
Soil Sample
Media
+
Orbital Shaker
For 2 Days
Enrichment
Acclimatization
Media
Concentration of
profenofos
100 ml 100 ml 100 ml 100 ml
control 50 ppm 100 ppm 150 ppm
Inoculum 1 ml 1 ml 1 ml
Serial Dilutions
Spreading
Incubation
For 2 days (48 hours) at 37 oC.
Plate Count
•Morphological Characterization
Colony Morphology (Microscopy) Cell Morphology (Through staining)
•Physiological Characterization
•Biochemical Characterization
•Molecular Characterization
Characterization
•Specific growth of pesticide degrading microorganisms
•Planning for isolation and Characterization in the next step
Conclusion
•Very good experience
•Applications of Microbiology in Environmental Engineering
•Would like to continue the research in the next semesters
Experience
Facilities in IESE Microbiology Lab
•Sterile Environment
•Well equipped
•Very cooperative and trained Staff
•Students/Researchers are always welcomed
Suggestions
• Separate lab for Bioremediation
• HEPA Filters
Professor Dr. Imran Hashmi
Miss Arish Naseem
Acknowledgement