Iodamoeba butschliiand

Endolimax nana

PRESENTED BY: PRINCESS ALEN AGUILAR

Iodamoeba butschliiGEOGRAPHICAL DISTRIBUTION: CosmopolitanPATHOGENESIS: Generally considered as non-

pathogenic amoeba.◦ THUS TREATMENT IS NONE

HABITAT: Large intestines.RESERVOIR HOST: Pigs. INFECTED FORM: Mature, uninucleated cyst.MODE OF INFECTION: Ingestion

(contaminated food or water)LABORATORY IDENTIFICATION:

cyst/trophozoite◦ Definitive diagnosis can only be made with the

iron hematoxylin or trichrome stained preparation

SPECIMEN SOURCE: Feces

Iodamoeba butschlii

Trophozoite◦ Positive identification of unstained

trophozoites is diffi cult because it can mimic E. nana

◦ reside in the large intestine where they survive by ingesting bacteria and yeast but not red blood cells.  

◦ In freshly prepared specimen Sluggishly progressive and has hyaline

pseudopodia

◦ In hematoxylin and trichome stain Vary in diameter from 4-20 um, but the majority

being within the range of 9-14um.

◦ Bacteria may be seen through out the cytoplasm

◦ Nucleus is usally not visible

Iodamoeba butschlii

Cysts◦Range in diameter from 6-16um (ave 9 or 10um).

◦Matured contains 1 nuclei, also the stage where it enters the host

◦If unstained, surrounded by a refractile wall. Irregular in shape instead of being

ovoid Glycogen vacuole is still prominent The nucleus is seldom distinct

Iodamoeba butschlii

Iodamoeba butschliiIodine Stain Characteristic

◦ Receives its generic name from the characteristic glycogen vacuoles of the cyst stage, which are so prominent that in iodine stains the cysts seem to contain little else. While glycogen vacuoles occur in other amoebae, they are never as regular in outline nor as consistently present an in Iodamoeba. With dark brown mass, often more than half the

diameter of the cyst with pale yellow cytoplasm

◦ The nucleus is seen at one side of the glycogen vacuole.

◦ The stain dissolve the glycogen

CYST

IODINE STAIN

Permanent stains◦  In well stained preparations, chromatin

granules may be form a crescent shape partially surrounding the karyosome. Linin fibrils may be seen running between the karyosome and the chromatin granules.  This arrangement when visible has been described as a ‘basket of flowers’, with a distorted karyosome forming the ‘basket’, the linin fibrils as the stems and the granules as the blossoms. 

◦ Reveal the characteristic nuclear structure ◦ Nuclear membrane is delicate◦ Karyosome is large, central in position,

irregularly rounded, and surrounded by a layer of small granules.

Iodamoeba butschlii

I. butschlii

Trophozoites, unstained Not characterictic

Trophozoites, stained Diagnostic: nucleus with large central karyosome surrounded by a ring of small chromatin granules; or nucleus structure as in cyst

Cysts, unstained Suggestive: large refractile body in cytoplasm; single nucleus

Cysts, stained Diagnostic: basket nuclei or nuclei as in trophozoite; large glycogen vacuole

Iron Hematoxylin stain 

I.bütschlii trophozoite showing large blotchy karyosome withing nucleus.  Nucleus outline visible showing no peripheral chromatin.

Iron Hematoxylin stain

I.bütschlii trophozoite - cell shape is more irregular that Endolimax nana which is usually rounder.

Iron Hematoxylin stain

I.bütschlii trophozoite - Binucleated cell

CYST

Nuclear membrane all but invisible.  Large glycogen vacuole seen on right side of cell.

CYST

Large glycogen vacuole takes up most of the cell's interior

CYST

binucleated cell

CYST

Concentrate (unstained)

CYST

BASKET OF FLOWERS, ESTER BASKET

ENDOLIMAX NANAThe most common of the smaller

intestinal amebaeusually encountered with about the

same frequency as in E. coliSize range for both trophozoites and

cyst is similar to that of E. hartmanni [(usual size, 4-12 µm) and cyst forms (usual size, 5-10 µm)], thus makes confusion in unstained preparations

Epidemiology is cosmopolitanColonizes the colon

ENDOLIMAX NANA

ENDOLIMAX NANATrophozoites

Range from 5-12 um in diameter (average size close to 7 um). Pseudopodia are blunt and hyaline; they are extruded rapidly as in E. histolytica but fail to produce the directional locomotion seen in that species.

No nuclear chromatin, large karyosome, relatively clean cytoplasm (may contain some debris); tremendous nuclear variation (can mimicEntamoeba hartmanni, Dientamoeba fragilis and Iodamoeba bütschlii).

ENDOLIMAX NANACysts

◦Exhibit about the same size range as the trophozoites.

◦Most frequently ovoid, but sometimes they are spherical or subspherical

◦Refractile wall which is evident in zinc sulphate technique Results the “SHRINKAGE” of the cytoplasm

of many cysts-effect also seen in Giardia

◦Possess 1-4 nuclei In immature binucleate cysts, the nuclei

are paired and located at one side, while in mature tetranucleate cysts the nuclei are paired and located at each end.

Endolimax nanaTrophozoites, unstained Not charateristic

Trophozoites, stained Diagnostic: a nucleus with large karyosome, generally with little or no peripheral chromatinSuggestive: Ovoid

Cysts, unstained Suggestive: Ovoid

Cysts, stained Diagnostic: 4 nuclei with large karyosome and little or no peripheral chromatin

Trichrome stain

The nuclei in this mature, quadrinucleated cyst have a large karyosome surrounded by a clear halo; peripheral chromatin is absent.

Wet mount examination of SAF-fixed specimen

The nuclear characteristics of this trophozoite are clearly visible

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