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Excitation Energy Transfer in Light Harvesting Complex II
Jiahao ChenDecember 15, 2004
PHYS 598 NSM
Plants as Transducers
Light energy Chemical potential energy The light harvesting process
threshold of photochemistry
M. Kamen, Primary Processes in Photosynthesis, Academic Press: NY, 1963.
This study
Light harvesting~ 0.5 – 100 ps
Light Harvesting Complex II Most common
photosynthetic protein in plants
Energy funneled to reaction center
Trimeric in vivo Study monomer
properties Components:
chlorophyll a (pink), chlorophyll b (grey), protein (cyan).
LHC-II from spinach. PDB code 1RWT.
Liu et. al., Nature, 428, 2004, 287-292.
Objectives
Quantum and Statistical Physics
How long does it take to harvest energy?
Chemical Biology
What is the efficiency of light harvesting?
The Origin and Nature of Excitation Light absorption Photon exciton
electronic excited state
Ground state
hole
electron
exciton
Excited state
occupied orbital
empty orbital
photon
Förster Theory
Approximations Time-dependent first-order perturbation
theory Fermi’s Golden Rule (Transition) Dipole-dipole interactions
only Optically accessible states only
Förster formula
How to Quantify Efficiency
Mean passage time Average time needed to traverse the
entire protein Quantum yield
Fraction of excitons that make it from start to end
Assume dissipation to be the only competing process
Computational Procedure
Atomic coordinates
Distances betweencenters of mass: Rij
Identity:chlorophyll a v. b
Transition dipolestrength, f
Orientation:k factor
Förster rate: kij
Quantum yield, Mean passage time:
results
PDB
literatureparameters
Dipole-dipole couplings in LHC-II
Energy transfer rates for LHC-II (II)
Fastest route
Energy ends upsloshing betweenthis pair
Results
Strongest dipole couplings lead to fastest transition rates
Light harvesting efficiency: 98.7% Excitons have half-life of 50 transitions! Excitons can travel long distances before
decaying Mean passage time: 13.52 ps
Average transition time: 0.97 ps
Conclusions
Numerical evidence support model of antenna complexes funneling energy to a reaction center Pair that excitation prefers to move in
one direction Chl b (650 nm) Chl a (670 nm) transition
Pair that excitation prefers to end up at Directional preference in exciton
transfer rates
Acknowledgements
TCBG Klaus Schulten Melih Şener
Martínez Group Todd Martínez Hanneli Huddock