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Building Biotechnology for a Knowledge-based Bioeconomy
Brain Storming Session Prioritization of Research for
Development of N-W Himalyan States 12-13 July 2013, GBPUAST Pantnagar
Dr Nazir A Ganai
Head, Division of Biotechnology
SKUAST-Kashmir
Agriculture and the Bieconomy
Contents:
A SWOT analysis of agriculture in J & K Status of agriculture in J & K
Challenges and Opportunities facing agriculture
Bioeconomy- the way forward?
What is it?
Why Bioeconomy ?
Global trends
Our Initiatives in Biotech applications
Recommendations
North-Westen Himalyan States
J & K: 2.22 lac Sq km
(65% )
H P: 0.55 lac Sq km
(18% )
Uttrakuand: 0.53 lac
Sq km (17% )
Land Resource in J & K ( Ha)
G-2,65,000
E-74,000 D- 1,05,000
C-2,11,300
B- 2,91,000
A - 6,58,000
F- 7,52,000
A Forest
B Non-Agricultural Land
C Barren & Uncultivable Land
D Permanent Pastures & Other Grazing Land
E Fallow Land Including Current Fallows
F Net Area Sown (31% of the land use area) or 7 % of total Geographic area
G Area under Fruit Crops
Contribution of different sectors to economy in J & K
46%
25%
30%
50%
35%
15%
J & K National
Industrial investment in J & K
National
Agribase economy in J & K
• the economy - bio-mass based.
• Subsistence requirements of the people for: – food grains,
– firewood,
– fodder,
– timber for housing,
– milk, meat, fibre and
– medicines are derived from the plant and animal resources available in the state.
Agribase economy in J & K • Inputs for industrial and commercial sectors - obtained
from the bio-mass produce of the state.
• Export of fresh and dry fruits,
• the famous saffron and honey,
• timber, resin, medicinal items,
• the basmati of Jammu region
• Famous handicrafts: • Pashmina shawls, carpets etc
• artefacts carved from walnut
• Tourism industry: tourists are attracted largely by the scenic natural beauty of the state with its lofty lush green mountain ranges, towering snow-clad peaks, placid sparking lakes, bubbling streams and springs.
Our strengths and opportunities
• Vast area: 2.22 lac sq kms (67% of N-W Himalya)
• Diverse and varied agro-climatic zones – Cold arid ladakh region – Temperate Kashmir region – Sub-tropical jammu region
• Rich Biodiversity – Ethnic diversity – Flora : 3,054 species. – Domestic & Wild life: 16% of india – Rich Medicinal & Aromatic plants
Strengths …….. • Unique Cash crops • Kashmir:
– Saffron – monopoly in india – Apple: 77% production in country – Walnuts: monopoly – Spices: Kashmiri mirchi, kala zeera, – Floriculture
• Jammu: – Basmiti- Quality – Rajmash – Black caraway (Zeera)
• Ladakh – Pashmina fibre – Apricot – Herbs related amchi system of medicine
Diversity
Temperature +40O C to - 40OC
Altitude 3000 ft to 24000 ft above MSL
Rainfall 110 mm Ladakh to 600 mm
Effect of herbal extracts on the cell
proliferation in Prostate cancer
cell line, C4-2 cells
Preserving and enhancing the indigenous plant knowledge is actually rescuing a global heritage
and is a recognized tool in search for new drugs and pharmaceutical sources, (Lambert et al.,
1997)
Nearly 50 per cent of the plant species described in British
pharmacopoeia are reported to grow in Kashmir valley
• Till date more than 5000 aromatic/medical plants have been discovered in
Jammu and Kashmir.
• Scientific observations reveal that the state could become the superpower in
future for the herbal medicines and perfumes if the huge resources are
exploited properly and effectively
Floral Diversity Floral Diversity
Animal Diversity Tree Diversity
Challenges facing agriculture
• Widening demand and supply gaps: – 40% import of food grains - 8 lac tones ( ~ Rs 9 billion)
– 20% vegetables - 1 lac tonnes (~ Rs 1 billion)
– 60% meat (sheep and goat) (~ Rs 3 billion)
• Increasing population pressure from highest growth rates in the country (2.7 % vs 1.6%). ,
• Shrinking land resources due to population expansion, urbanisation and soil degradation, indiscriminate & unplanned growth of housing sector
• Fragile Climate: Extreme winters, long dry spells,
• Rain fed agriculture due to peculiar topography
• Receding glaciers due to global warming further aggravate the problems inherent to the rain fed agriculture J & K,
----challanges
• deforestation, loss of biodiversity and qualitative deterioration of the pastures
• indiscriminate use of chemical fertilisers and pesticides contaminating food, soil and water beyond minimum permissible levels.
• over exploitation of natural resource base and environmental quality decline,
• global warming and climate change leading to emergence of new biotic and abiotic stresses
• stagnation in productivity of agricultural crops • Subsistence agriculture ( less renumerative)
Vanishing “Venice of Asia” , Dal Lake disappearing (National Geographic News, June 9, 2010)
Deweeding of Dal Lake costs millions of rupees to Kashmir budget
In 20th Century “Dal Lake” represented a goldmine of Tourism in Kashmir
Biotechnology has a solution for ‘dying Dal Lake’
Grass Carp, a
voracious grass eater
fish, when genetically
modified in china ,
resulted in 42%
increase in growth
rate and 16%
increase in grass
consumption
Transgenic carp
Chinese Science Bulletin 2003 Vol.
48 No.16 1764 1770
Sustainable production of
renewable biomass?
PM Dr. Manmohan Singh at G. B. Pant University of Agriculture and Technology on June 19, 2010, said…
“ policymakers and scientists need to put their heads together for developing sustainable technologies that can produce more from less in the background of the
new challenges of global warming and climate change…”
The bioeconomy : the way forward
the sustainable production of renewable biomass which includes any biological material to be used as raw material and
conversion of biomass into a range of food, health, fibre and industrial products and energy
can play an important role in both creating economic growth, and in formulating effective responses to pressing challenges- food /nutritional security, clean environment and human health
Way forward - KBBE
• Convergence of Biotechnology , nanotchnology and bioinformatics
• provides the knowledge-base for the sustainable management, production and use of biological resources…
• provides new, safe, affordable and eco-efficient products …
• Through • Shift in practice from a sectoral approach to our problems
towards a more integrated approach of the KBBE.
• Knowledge and innovation in bilogical sciences
Biomass
feedstockAnimal and plant
therapeutics
and diagnostics Nutraceuticals
and
pharmaceutical
production
Fine chemical
production
Research on
genomes, cell
processes, and
bioinformatics
Health:
new therapies
and diagnostics
Industry:
enzymes, biofuels,
and bioplastics
Primary production:
food, feed, fibre,
and cellulosic crops
Integration across Biotechnology Applications
Our Initiatives and Successes in Biotech Applications
-- fragmented nd isolated attempts
Biotechnological Interventions for Improving Reproductive Performance
• Somatic Cell Nuclear Transfer (SCNT) in Goats & Sheep
• Simplified/Modified zona-free Cloning Technique
“First Cloned Pashmina Goat “NOORI” born at Division of Biotechnology, SKUAST-K, Srinagar, through this Technique”.
“Noorie” with her foster dam •
“Noorie” at 1 year of Age •
Enucleated Eggs
Reconstructed embryos cultured in lab for 7 days
Blastocyst
Clones
Goat ovaries
Donor cell culture
Somatic cell donor
Oocyte –somatic cell fusion
Foster mother
PRODUCTION OF CLONED PASHMINA GOAT AT SKUAST KASHMIR
Incubation (38.5 C)
Cloning. Laboratory, Centre of Animal Biotechnology, SKUAST-K, Srinagar
Awards & Achievements
PI of the Project receiving cerificate of appreciation from DG, ICAR and
Minister for Agriculture, J&K
J&K Tableau on Republic Day 2013 depicting Centre of Biotechnology, SKUAST Kashmir and its
research achievements
PI being awarded by the Chief Minister Omer Abdullah
Bioprospecting of genes for cold tolerance
Pashmina Goat Bactrian Camel
Abiotic Stress Factor : Cold Stress
Heatmap analysis of differentially
expressed genes in pashmina
goat under Cold vs Hot
conditions in Blood and Skin
Important candidate genes identified
1. ADRB: Adrenoegic receptor binding
2. CIRBP: cold induced RNA binding
3. RBM3: RNA binding motif
4. UCP1 : Uncoupling protein
5. HIF-1 Hypoxia inducible Factor
6. GAS7 : Growth arrest specific
7. IGF1: Insulin like growth factor
8. LSP lympocyte specific protein
9. PAM: Neurotransmitter
10.HSP105
11.HSP47
12.HSP70-1
Figure 2: Ven diagram representing the number of transcripts in skin samples of pashmina goats whose changes in expression during different seasons.
Camel Genome Information Resource
A second database on complete
transcriptome of ~ 21000 transcripts is
under development.
Other Important Achievements
• Vaccine against foot rot in sheep and goat
– Increased Fecundity in Sheep
• M A Introgression of FecB gene
• Lambing percentage increase from 80% to 150
•
Apple industry-
India : 7th largest producer of apple
Kashmir: 70% to apple production
At a cost of : 7000 Tons of fungicides annually
Concerns: Impacts Human Health & Environment,
future marketing oppurtunities
Challenge:
Breed for disease resistance
Develop Bio control agents for disease management
State Area (000 HA)
Production (ooo MT)
Productivity (MT/HA)
J & K 133 1330 10
HP 97 510 5
Uttranchal 32 132 4
Biopesticides developed in SKUAST-K:
Biowilt- X (Trichoderma harzianum) Biocomp-X (Pseudomonas fluorescens)
USA Patent No: (No. US 7, 815, 903 B2; Date of Grant 19-10-2010) and
India Patent No: (239609; Date of Grant 26-03-2010).
Biofertilizers for organic agriculture
1. Phosphate solubilizing bacteria – Makes available 30 kg of phosphorus
to the crops per hectare per year.
– Improves crop growth by secretion of growth regulators.
– Seed treatment : Mix 250 ml with the seed needed for one acre and dry for 20 minutes under shade before
2. Shalimar Microbes:
• Consortium of different microbes like phosphate solubilizing bacteria,
actinomycete, Lactobacillus.
• Use: solid waste decomposition and
also acts as a biofertilizer.
SHALIMAR BIOFERTILIZER (Azotobacter) • Fixes 20-40 kg nitrogen from atmosphere per
hectare per year.
• Increases yield by 10 – 25%.
• Improves crop growth by secretion of growth regulators and vitamins.
• Protects crops against some soil borne pathogens.
• Seed treatment: : 250ml/acre
• Seedling root dipping : 1000ml/acre
• Field application : 400ml/acre (Mix the biofertilizer with the soil before sowing
• Suitable for crops : Wheat, oat, barley, Mustard, seasum, Linseeds, Sunflower, castor, Pearl millets, Finger millets, and floriculture plants etc.
SHALIMAR BIOFERTILIZER (Rhizobium phaseoli)
• Features: • Fixes 100 to 300 kg nitrogen per hectare
in one crop season.
• Increases yield by 10-35%.
• Improves crop growth by secretion of growth regulators.
• Protects crops against some soil borne pathogens.
• Seed treatment : 250ml/acre
• Field application : 400ml/acre (Mix the biofertilizer with the soil before sowing).
• Suitable for crops Rajmash
E-learning portal
www.starelearning.org
MIS developed for:
1. FARMMAN 1.00 Management
of Cattle and buffalo breeding
research data
2. SheepMan 1.00: Online tool
for management of sheep
research data across farms
3. SoftMate: Tool for planning
breeding programs to avoid the
inbreeding depression
New Initiative: Dairy Development – Challenge of 21st Century
Demand- High Nutritional security to 1.6 billion people by 2030 Rising purchase power Improving health conciousnes Support to Dairy Industry
Challenge for 2030 4 fold growth: 127 million tones to 550 million tones
by (300 ml to 1000 ml person / day 5 fold growth: 14 lac tons to 74 lac tones
Constraint Acute dearth of proven germplasm Feed and Fodder availability Health cover
Dairy Development: Integrating
Biotechnology and IT
e-linking of farmers cows with the hub (elite
nucleus herd) for pursuit of ONBS-MOET
Approach…..
e-registration of farmers cows for :
delivery of services and collection of feed back data
Widening of the genetic base from few hundred farm-bred cattle to 7 lac breedable cows of farmers through e-linking for planning long-term research programs for sustainable dairying
Data Warehousing to support data analysis and decision-making tasks through use of ICT.
Production of tested high performance breeding bulls through:
Open Nucleus Breeding Scheme t
E T T for fast multiplication of elite cows
GWAS for Evaluation of breeding value
Recommendations
• Strengthening of the capacity (infrastructure and human resource) in J & K to get ready for the technology driven bio-revolution
• Special program for Networking of the institutes in the three states on N-E pattern for:
– Knowledge and Resource sharing
• Faculty exchange & Student exchange
– Documentation and characterization of our bioresources
• Common e-resource of our biodiversity – validated and authentic
– Bioprospecting of our resources for
• commercially valuable genetic and biochemical resources.
• Genes for biotic and abiotic stress tolerence
Recommendation………
• Collaborative inter-institutional research programs
– MAS for accelerated improvement in field / horticulture crops / animals / fish
– MA S approach for breeding resistance in plants and animals
– Mitigating Climate change and ensuring healthy foods:
• Development of biocontrol agents for management of diseases and pests
• Development of the biofertilisers for organic agriculture