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LE 6-5A
Homogenization
HomogenateTissuecells
Differential centrifugation
• Tissue is cut into pieces and placed in cold isotonic buffer solution.
• Pieces of tissue are ground into very small fragments in a type of liquidiser called as homogeniser.
• The suspension of cell fragments is filtered to remove large pieces of tissue debris
• The filterate is then placed in a centrifuge