VISOCOLOR®
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Manual VISOCOLOR® reagent case for soil analysis
Manual VISOCOLOR® reagent case for soil analysis 03.14MN
Contents
1. VISOCOLOR® reagent case for soil analysis ……………………………………………………………… 24
1.1 Analysis options ……………………………………………………………………………………………………………………… 24
1.2 Content of the VISOCOLOR® reagent case for soil analysis (REF 931 601) ……………………………………………… 24
1.3 Content of the VISOCOLOR® reagent case for soil analysis with PF-3 (REF 934 220) ………………………………… 25
1.4 Working procedure ………………………………………………………………………………………………………………… 26
2. Soil analysis procedure ……………………………………………………………………………………… 27
2.1 Sampling ……………………………………………………………………………………………………………………………… 27
2.2 Weighing and determination of moisture content ……………………………………………………………………………… 27
2.3 Sieving the soil sample ……………………………………………………………………………………………………………… 27
2.4 Determination of the soil density ………………………………………………………………………………………………… 27
2.5 Preparation of soil extract A ……………………………………………………………………………………………………… 28
2.6 Determination of the pH value …………………………………………………………………………………………………… 28
2.7 Determination of nitrate and nitrite ……………………………………………………………………………………………… 29
2.8 Determination of ammonium ……………………………………………………………………………………………………… 29
2.9 Preparation of soil extract B ……………………………………………………………………………………………………… 29
2.10 Determination of phosphorus …………………………………………………………………………………………………… 30
2.11 Determination of potassium ……………………………………………………………………………………………………… 30
2.12 Determination of soil type (sedimentation analysis) ………………………………………………………………………… 31
3. Procedure for photometric soil analysis …………………………………………………………………… 31
3.1 Preparation of soil extract AF ……………………………………………………………………………………………………… 31
3.2 Determination of pH value ………………………………………………………………………………………………………… 31
3.3 Photometric determination of nitrate …………………………………………………………………………………………… 32
3.4 Photometric determination of nitrite ……………………………………………………………………………………………… 32
3.5 Photometric determination of ammonium ……………………………………………………………………………………… 32
3.6 Preparation of soil extract B ……………………………………………………………………………………………………… 33
3.7 Photometric determination of phosphorus ……………………………………………………………………………………… 33
3.8 Photometric determination of potassium ……………………………………………………………………………………… 34
4. Calculation and correction of results ……………………………………………………………………… 35
4.1 Correction of moisture content …………………………………………………………………………………………………… 35
4.2 Calculation referring to areas ……………………………………………………………………………………………………… 35
4.3 Conversion into different dimensions …………………………………………………………………………………………… 35
Manual VISOCOLOR® reagent case for soil analysis
MNManual VISOCOLOR® reagent case for soil analysis 03.14
1. VISOCOLOR® reagent case for soil analysis
NANOCOLOR®
NANOCOLOR®
1.1 Analysis options
Extraction solutions
·
·
·
·
Individual parameters Analysis REF®
®
VISOCOLOR® ECO
VISOCOLOR® ECO
VISOCOLOR® ECO
VISOCOLOR® ECO
VISOCOLOR® HE
VISOCOLOR® HE
1.2 Content of the VISOCOLOR® reagent case for soil analy-
sis (REF 931 601)
VISOCOLOR®
5
9 9
10
20
19 19
18
30
258 8
26
29
16
3231 31
37 37 3836 36
33 34 35
39
22111213
6
2423
19
17
17
2
2827
1421
43
15
15
6
7
7
1
Manual VISOCOLOR® reagent case for soil analysis
Manual VISOCOLOR® reagent case for soil analysis 03.14MN
1.3 Content of the VISOCOLOR® reagent case for soil analy-
sis with PF-3 (REF 934 220)
VISOCOLOR®
5
9 910
30
8 826
11
13
5032 49
47 4745 46
42 43 44
18
22
6
5123
12
51 51 51
17
17
2
4140
14
2143
15 15
6
7
7
1
282739
No. Item REF
HE
HE
®
®
HE
HE
HE
HE
ECO
ECO
ECO
ECO
ECO
ECO
ECO
ECO
ECO
ECO
ECO
48
Manual VISOCOLOR® reagent case for soil analysis
MNManual VISOCOLOR® reagent case for soil analysis 03.14
1.4 Working procedure
(see 2.6, page 28) (see 3.3, page 32)
(see 2.7, page 29)
NH
(see 3.5, page 32)
NH
(see 2.8, page 29)
(see 2.10, page 30)
(see 3.7, page 33)
(see 2.11, page 30)
(see 3.8, page 34)(see 4, page 35)
Manual VISOCOLOR® reagent case for soil analysis
Manual VISOCOLOR® reagent case for soil analysis 03.14MN
2. Soil analysis procedure
2.1 Sampling
(see 2.2, page 27, see
2.4, page 27)
2.2 Weighing and determination of moisture content
Determination of moisture content:
[ ] [ ]
[ ]
210 x 279 mm
50
100
150
200
250250 ml
pp
C
MAX
pp
135
MAX
AP
PR
OX
IMA
TE
VO
LU
ME
S
2.3 Sieving the soil sample
210 x 297 mm
2.4 Determination of the soil density
Procedure:
ml 100
90
80
70
60
50
40
30
20
10
Calculation:
[ ]
Manual VISOCOLOR® reagent case for soil analysis
MNManual VISOCOLOR® reagent case for soil analysis 03.14
2.5 Preparation of soil extract A
Preparation of the extraction solution:
2
4
6
8
10ml
B.B
RA
UN
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
EXP
100 mL
CaCl2-VorratslösungCaCl2 stock solution
Concentré CaCl2Solución concentrada CaCl2
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
1 L
Gebrauchslösung ACaCl2-Lösung
zur BodenuntersuchungExtraction solution A
CaCl2 solution
for soil determination
Solution d’extraction A Solution CaCl2 pour
la détermination de sol
Solución de extracción A Solución CaCl2 para
la determinación de suelo
®
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
Destilliertes Wasser
Distilled water
Eau déstillée
Agua destilada
Preparation of the soil extract:
non-dried
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
1 L
Gebrauchslösung ACaCl2-Lösung
zur BodenuntersuchungExtraction solution A
CaCl2 solution
for soil determination
Solution d’extraction A Solution CaCl2 pour
la détermination de sol
Solución de extracción A Solución CaCl2 para
la determinación de suelo
50
100
150
200
250250 ml
pp
C
MAX
pp
135
MAX
AP
PR
OX
IMA
TE
VO
LU
ME
S
www.mn-net.com
MN 616 ¼ · Ø 185 mm
100 REF 532 018
ml 100
90
80
70
60
50
40
30
20
10
2
4
6
8
10ml
B.B
RA
UN
50
100
150
200
250250 ml
pp
C
MAX
pp
135
MAX
AP
PR
OX
IMA
TE
VO
LU
ME
S
2.6 Determination of the pH value
Procedure:
VISOCOLOR® HE
8.5
8.0
7.5
7.0
6.5
4.0
5.0
5.5
6.0
9.0
10.0
VISOCOLOR HE pH 4.0 – 10.0
Art.-Nr.
920 374
®
pH 4–104 x
HE
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
®
pH 4–104 x
HE
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
Manual VISOCOLOR® reagent case for soil analysis
Manual VISOCOLOR® reagent case for soil analysis 03.14MN
Note: For measurements with an electrometric pH meter, a
special soil extract A is prepared in the ratio 2 + 5, e.g. 20 g
soil + 50 mL extraction solution A. You may also use soil extract
AF (see 3.1, page 31).
5 mL
www.mn-net.com
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
pH-Fix 2.0–9.0
100 farbfixierte Indikatorstäbchen für viele Anwendungen100 color-fixed indicator strips for multiple applications
auch geeignet für die pH-Bestimmung von Magensaft
also suitable for the pH determination of gastric juice
Normale Anwendung: Eintauchen – ablesen! Bei schwach ge-pufferten Lösungen Stäbchen längere Zeit (1–15 min) eintauchen.General use: Dip in – read off! If solution is weakly buffered leave test strip in solution for a longer time (1–15 min).
\EXP 2015-06LOT 18B116
REF
921 18
A011554 / Rev. 2012-04
4 °C
30 °C
2.7 Determination of nitrate and nitrite
®
0
1 mg/
5
10
20
40
80
®
0
10
25
50
100
250
500
Procedure:
Please note: Reclose the package tightly immediately after use.
Calculation of results:
2.8 Determination of ammonium
®
0
10 mg/L
25
50
100
200
400
®
5 mL
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
10 x
NH4+–1
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
10 x
NH4+–1
Procedure:
Close ammonium vial immediately after removing the test strip.
Calculation of results:
2.9 Preparation of soil extract B
Preparation of the extraction solution:
B, the solution is to be discarded. Rinse the bottle several times
with hot water and prepare fresh solution.
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
EXP
100 mL
CAL-VorratslösungCAL stock solution
Concentré CAL
Solución concentrada CAL
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
1 L
Gebrauchslösung BCAL-Lösung
zur BodenuntersuchungExtraction solution B
CAL solution
for soil determination
Solution d’extraction B Solution CAL pour
la détermination de sol
Solución de extracción B Solución CAL para
la determinación de suelo
®
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
Destilliertes Wasser
Distilled water
Eau déstillée
Agua destilada
Preparation of the soil extract:
Manual VISOCOLOR® reagent case for soil analysis
MNManual VISOCOLOR® reagent case for soil analysis 03.14
www.mn-net.com
MN 616 ¼ · Ø 185 mm
100 REF 532 018
ml 100
90
80
70
60
50
40
30
20
10
2.10 Determination of phosphorus
VISOCOLOR® ECO
Procedure:
®
P-16 x
HE
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
®
P-26 x
HE
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
®
P-K6 x
HE
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
Phosphor in Boden / Phosphorus in soil
Phosphore dans le sol / Fósforo en suelo
REF
P
D 04721 / A020806 / 9
20 383 /
100
3
920 3831 - 20 mg P / 100 g
20
16
12
8
6
4
3
2
1
0
2.11 Determination of potassium
Procedure:
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
K-2 1 x
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
K-115 x
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
K-115 x 15
10
8
6
4
3
2
Ka
lium
/ P
ota
ssiu
m
m
g/L
K+
Ka
lium
/ P
ota
ssiu
m
m
g/L
K+
Ka
lium
/ P
ota
ssiu
m
m
g/L
K+
Ka
lium
/ P
ota
ssiu
m
m
g/L
K+
3 mg/L
Calculation of results:
Manual VISOCOLOR® reagent case for soil analysis
Manual VISOCOLOR® reagent case for soil analysis 03.14MN
Forest sedimentation analysis according to KRUEDENER
2.12 Determination of soil type (sedimentation analysis)
Procedure:
E
F
10 x
®
Neumann-Neander-Str. 6-8 · D-52355 Düren
MACHEREY-NAGEL
PyrophosphatlösungPyrophosphate Solution
Solution de pyrophosphateSolución de pirofosfato
Example:
-
timation regulation [3] [6]
Mark Sand (%) Soil type
E
D
3. Procedure for photometric soil analysis
VISOCOLOR®
VISOCOLOR®
NANOCOLOR®
NANOCOLOR® NANOCOLOR®
3.1 Preparation of soil extract AF
see 2.5, page 28
Procedure:
3.2 Determination of pH value
Procedure:
VISOCOLOR® HE
NANOCOLOR®
Manual VISOCOLOR® reagent case for soil analysis
MNManual VISOCOLOR® reagent case for soil analysis 03.14
3.3 Photometric determination of nitrate
Procedure with PF-3:
VISOCOLOR®
ECO
1
2
3
4
5ml
B.B
RA
UN
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
NO3-15 x
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
NO3-2 1 x
Preprogrammed submethods:
NANOCOLOR®
Preprogrammed submethods:
Procedure with further NANOCOLOR® photometers
NANOCOLOR®
NANOCOLOR®
Preprogrammed NANOCOLOR® photometers
NANOCOLOR® UV/VIS, VIS, 500 D, 400 D, 300 D, 250 D, PF-12
Other photometers
3.4 Photometric determination of nitrite
NANOCOLOR®
Preprogrammed photometers
NANOCOLOR® UV/VIS, VIS, 500 D, 400 D, 300 D, 250 D, PF-12
Other photometers
3.5 Photometric determination of ammonium
Procedure with PF-3:
VISOCOLOR®
ECO
NH
1
2
3
4
5ml
B.B
RA
UN
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
NH4-21 x
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
NH4-3 4 x
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
NH4-110 x
Preprogrammed submethods:
NANOCOLOR®
Manual VISOCOLOR® reagent case for soil analysis
Manual VISOCOLOR® reagent case for soil analysis 03.14MN
Preprogrammed submethods:
Procedure with further NANOCOLOR® photometers
NANOCOLOR®
Preprogrammed NANOCOLOR® photometers
NANOCOLOR® UV/VIS, VIS, 500 D, 400 D, 300 D, 250 D, PF-12
Other photometers
3.6 Preparation of soil extract B
Preparation of the extraction solution:
B, the solution is to be discarded. Rinse the bottle several times
with hot water and prepare fresh solution.
Preparation of the soil extract:
3.7 Photometric determination of phosphorus
Procedure with PF-3:
VISOCOLOR®
ECO
VISOCOLOR® ECO
1
2
3
4
5ml
B.B
RA
UN
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
PO4-16 x
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
PO4-2 6 x
Preprogrammed submethods:
NANOCOLOR®
Preprogrammed submethods
Manual VISOCOLOR® reagent case for soil analysis
MNManual VISOCOLOR® reagent case for soil analysis 03.14
Procedure with further NANOCOLOR® photometers
NANOCOLOR®
Preprogrammed NANOCOLOR® photometers
NANOCOLOR® UV/VIS, VIS, 500 D, 400 D, 300 D, 250 D, PF-12
Other photometers
3.8 Photometric determination of potassium
Procedure with PF-3:
VISOCOLOR® ECO
1
2
3
4
5ml
B.B
RA
UN
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
K-2
Neumann-Neander-Str. 6–8 · 52355 Düren
Germany · Tel.: +49 24 21 969-0
K-1 15 x 1 x
Preprogrammed submethods:
NANOCOLOR®
Preprogrammed submethods
Preprogrammed NANOCOLOR® photometers
NANOCOLOR® UV/VIS, VIS, 500 D, 400 D, 300 D, 250 D, PF-12
Other photometers
Manual VISOCOLOR® reagent case for soil analysis
Manual VISOCOLOR® reagent case for soil analysis 03.14MN
4. Calculation and correction of results
4.1 Correction of moisture content
Calculation:
Factors for correction of soil moisture
CaCl2
A 1 + 1
(see 2.2, page 27)
CAL
B 1 + 20
(see 2.2, page 27)
CaCl2
AF 1 + 2
(see 2.2, page 27)
Example:
4.2 Calculation referring to areas
(see 2.1,
page 27)
Calculation: M x d x f x D x CF = R
M [ ]
d [ ]
f [ ]
D [ ]
CF [ ]
R [ ]
Example 1: Example 2:
4.3 Conversion into different dimensions