Download pdf - U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read Buffer T ... O-PLEX Spot . Title: U-PLEX_Human_Grp1_Custom_Quick_Guide Author: Windows

Transcript

Page 1: U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read Buffer T ... O-PLEX Spot . Title: U-PLEX_Human_Grp1_Custom_Quick_Guide Author: Windows

≤

≤

U-PLEX®

Custom Biomarker (Human) Assays

Components

,

Quick Guide

Additional Materials

https://www.mesoscale.com/en/technical_resources/search_all_documents/?031aed612ac74f579d7e0bdb82fb3089=906ce4b4dcf847f6b883cb83a4d02749

http://www.mesoscale.com/

Page 2: U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read Buffer T ... O-PLEX Spot . Title: U-PLEX_Human_Grp1_Custom_Quick_Guide Author: Windows

Reagent Preparation

Prepare Conjugated Capture and Detection Antibodies

Prepare the U-PLEX Plate

Step 1 Create the Individual U-PLEX-Coupled Antibody Solutions

≥

Step 2 Prepare the Multiplex Coating Solution

https://www.mesoscale.com/en/technical_resources/search_all_documents/?031aed612ac74f579d7e0bdb82fb3089=906ce4b4dcf847f6b883cb83a4d02749

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Page 3: U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read Buffer T ... O-PLEX Spot . Title: U-PLEX_Human_Grp1_Custom_Quick_Guide Author: Windows

Step 3 Coat the U-PLEX Plate

Prepare Calibrator Standards

≤

Reagent Preparation

Page 4: U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read Buffer T ... O-PLEX Spot . Title: U-PLEX_Human_Grp1_Custom_Quick_Guide Author: Windows

Reagent Preparation/Assay Protocol Dilute Samples

Prepare Detection Antibody Solution

Prepare 2X Read Buffer T

Step 1 Add Samples and Calibrators

Step 2 Wash and Add Detection Antibody Solution

Step 3 Wash and Read

MSD Customer Service MSD Scientific Support

http://www.mesoscale.com/

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