WELCOME TO MY
PRESENTATION
Presented ByMd. Rezaur Rahman
REGISTRATION NO.: 1305099
SupervisorDr. Md. Faruk Islam Associate Professor
DEPARTMENT OF MEDICINE, SURGERY AND OBSTETRICS HAJEE MOHAMMAD DANESH SCIENCE & TECHNOLOGY
UNIVERSITY, DINAJPUR
SEROPREVALANCE AND EFFICACY OF COMMERCIAL VACCINE AGAINST NEWCASTLE
DISEASE IN LAYER BIRDS
Co SupervisorDr. Md. Shajedur Rahman Assistant Professor
Newcastle Disease is one of the most important infectious disease of poultry in Bangladesh
It is still considered a major problem, because of its high morbidity, mortality particularly in rural areas and reduce productivity and economic loss in layer farm
Velogenic Strain Cause Mortality up to 90% and Morbidity near 100%
Prevalence of ND in different areas of Bangladesh are 50%, 45%, 25% and 32.5%.
BACKGROUND
Determination of seroprevalence of Newcastle Disease (ND) by Hamagglutination Inhibition (HI) test
Evaluation of immune response of different commercial vaccines in grower and layer birds.
OBJECTIVES
MATERIALS & METHODS
Study area: The study was carried out at different layer
farms of three upazilla namely- Kaharol, Bochagonj and Setabgonj in Dinajpur district
Total Number of Sample: 20 blood samples from each farm
Study period: January-June, , 2015
MATERIALS & METHODS
Study farm and population:Study farm and population:
Six (06) farms and total population of birds are 6000
Laboratory examination:
Toufiq Agro Laboratory, RangpurToufiq Agro Laboratory, Rangpur
MATERIALS & METHODS
SL No.
Name of Vaccine Strain Company
01 Avinew® VG/GA Strain Merial
02 Cevac New L® LaSota strain ACI
03 Newcastle Disease vaccine®
Mesogenic strain Novartis
MATERIALS & METHODS
Phosphate Buffer Solution (PBS) Newcastle Disease Vaccine Experimental Chicken Disposable Syringe Micropipette 96 Well micro titre plate Glassware and Appliances Chicken Red Blood Cell (cRBC) Suspension
MATERIALS MATERIALS & METHODS
Fig: Study Flow chart
VACCINES
Select of farms
Collection of 20 blood sample from each farm for Haemagglutination inhibition (HI) test to know Ab
titire
HI perform at Toufic agro lab. To determine the Seroprevalence
Selection of farms for vaccination
EXPERIMENTAL LAYOUT
Vaccination: Farm A --- New Castle Diseases Vaccine (Mesogenic Strain) Farm C --- Avinew (VG/GA Strain) Farm D--- Cevac new-L (Lasota)
20 blood serum from each farm for determination of Ab titre (20 × 3 = 60) at
Day7 and Day14
Evaluation and comparison of vaccine effectiveness against ND
EXPERIMENTAL LAYOUT
RESULTS
Table A. Seroprevalence of Newcastle Disease in grower farms
Ab = Antibody
Farms
Grower birdsOverall
prevalenceAge of birds (wks)
Total serum tested
Mean Ab titre
levelPositive
casePrevalence
among tested cases
A 16 20 23.6 5 25%
18.33%B 12 20 28.9 0 0%
C 14 20 23.9 6 30%
Farms
Layer birds
Overall prevalenceAge of
birds (wks)
Total serum tested
Mean Ab titre
levelPositive
casePrevalence
among tested cases
D 27 20 25.67 35%
23.33%E 22 20 25.84 20%
F 40 20 23.63 15%
Table B Seroprevalence of Newcastle Disease in layer farms
Table C. Ab titre level of 3 farms at Day0 (Day of vaccination)
(HI test at experimental “Day 0” at Geometric mean log2)(C-Mamun poultry, D- Masud poultry, A- Rakib poultry)
Farms StageNo. of blood
serum sample tested
Mean of Titre (Before Vaccination)
Farm-C Grower 20 23.9
Farm-A Layer 20 25.6
Farm-D Grower 20 23.6
Table D. Evaluation of vaccine efficacy after Day 7 and Day 14 day of following vaccination by different vaccine in
different ages layer farms
(Farm C-Mamun poultry, D- Masud poultry, A- Rakib poultry)
Table E. Comparative study of Immune response at grower farms
NS= Not Significance
Farms(grower) Vaccine name HI (D7) titre
After VaccinationHI (D14) titre
After Vaccination
A Avinew (VG/GA strain) 27.6 28.1
CNewcastle disease vaccine (Mesogenic
strain)27.2 28.0
P Value (t-test) NS NS
CONCLUSIONS
Detection of seroprevalance of ND in layer birds may assist to reduced prevalence of ND in layer farm through proper vaccination.
The titre level in farm C (Avinew VG/GA strain), A (Mesogenic strain) and D (Cevacnew-Lasopa) on Day7 and Day14 was 27.2 ,28
; 27.6 , 28.1 and 29.1 , 29.8 , respectively in three farms Cevac New L (LaSota strain) may be the choice of vaccine
against ND for layer farms though others may also be used. Further study may need to conduct in large scale of birds using
all commercial vaccine for recommendation.
THANKS TO ALL