YMC GENERAL CATALOG
2Reversed-Phase Columns(Other than ODS)
Types and characteristics of reversed-phase columns (other than ODS)
YMC-Pack Pro C8、C4YMC-Pack C8
YMC-Pack C4
YMC-Pack TMSYMC-Pack PhYMC-Pack CNYMC-Pack PROTEIN-RPYMCbasicYMC Carotenoid
58,59
60~6364,6566,6768,6970,7172,7374,75
7677
57
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58
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Types and characteristics of reversed-phase columns (other than ODS)
Product NamePore size(Å)
Particlesize
(µm)C% Silanol
treatmentUsable
pH range Characteristics Page
Pro seriesPro C8 120 3, 5 10 Yes
2.0 ~ 7.5
・ Processed with advanced endcapping technology
・ Superior separation of basic compounds 60 ~ 63
Pro C4 120 3, 5 7 Yes・ Processed with advanced
endcapping technology・Different selectivity from ODS
YMC-Pack seriesC8
120 3, 5,10 10Yes ・Moderate hydrophobicity 64,65200 5,10 7
300 5,10 4
C4
120 3, 5,10 7Yes ・ Lower hydrophobicity than
ODS and C8 66,67200 5,10 5300 5,10 3
TMS 120 3, 5,10 4 Yes ・ Reversed-phase packing material with the lowest hydrophobicity 68,69
Ph 120 3, 5,10 9 Yes ・ Reversed-phase packing material with π electrons 70,71
CN 120 3, 5,10 7 Yes ・ Can be used in both normal-phase and reversed-phase modes 72,73300 5 3
PROTEIN-RP 200 5 4 — 1.5 ~ 7.5 ・ Useful for separation of proteins and peptides 74,75
YMCbasic 200 3, 5 7 Yes 2.0 ~ 7.5 ・ Superior separation of basic compounds 76
YMC Carotenoid — 3, 5 — — 2.0 ~ 7.5 ・ Useful for carotenoids separation 77
Elution behavior dependent on alkyl chain lengthIn reversed-phase chromatography, retention due to hydrophobicity generally depends directly on the carbon number of the stationary phase. The degree of retention due to hydrophobicity of the stationary phase can generally be listed in descending order by column type as ODS>C8>C4>TMS. Stationary phases with low hydrophobicity can be used effectively to reduce analysis time for samples having too strong of a retention on ODS. Stationary phases with low hydrophobicity are also useful for samples that are slightly soluble in organic solvents and need to be analyzed with mobile phase containing a low concentration of organic solvents.
Types of reversed-phase columns(Ⅰ)
ODS -C18H37 Retention due to hydrophobicity
High
Low
C8 -C8H17
C4 -C4H9
TMS -CH3
Elution behavior affected by other factorsCN and Ph have available π electrons derived from their functional groups. CN and Ph sometimes show different separation characteristics from stationary phases that are chemically bonded with straight alkyl chains. Since CN has medium-polar functional groups, it can be used in both normal-phase and reversed-phase separation modes, depending on the mobile phase used.
Types of reversed-phase columns(Ⅱ)
Ph πelectrons available
CN -(CH2)3-CNπelectrons
availableCan be used also in normal-phase
Types and characteristics of reversed-phase columnsYMC-Pack Pro C8、C4YMC-Pack C8、C4、TMS、Ph、CN
YMC-Pack series include a variety of reversed-phase columns other than ODS, enabling column selection from a wide range of products to suit the sample characteristics.
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59
Reversed-P
hase Colum
ns (Other than O
DS
)
2
Elution behavior dependent on alkyl chain length(1)
1. Uracil2. Methyl benzoate3. Progesterone4. n-Butyl benzoate5. n-Butylbenzene6. n-Amylbenzene
Column :150×4.6mmI.D.Eluent :acetonitrile/water(60/40)Flowrate :1.0mL/minTemperature:37℃Detection :UVat260nm
2
13
45
6
100 20 30
YMC-Pack ODS-A
2
13
4
5
6
10 20 300
YMC-Pack C8
24
5
6
3
1
10 200
YMC-Pack C44
2
1
5
63
10 200
YMC-Pack TMS
Elution behavior dependent on alkyl chain length(2)
1. Toluene2. Deoxycorticosterone acetate3. Imipramine hydrochloride4. Triphenylene5. n-Amylbenzene
Column :150×4.6mmI.D.Eluent :20mMKH2PO4-K2HPO4(pH6.9)/methanol(25/75)Flowrate :1.0mL/minTemperature:37℃Detection :UVat254nm
0 5 10 15 20 25min
1
2
34
5
Pro C18
0 5 10 15 20min
1
2
3
4
5
Pro C8
0 5 10min
1
2
3
4
5
Pro C4Inthisexample,theretentionbehaviorofavarietyofcompoundsisshowntobedependentonthealkylchainlengthofthestationaryphase.ShorteralkylchainlengthslikeC4showreducedretentionforneutralcompoundsduetothediminishedhydrophobicityoftheC4stationaryphaserelativetolongeralkylchainsphaseslikeC8andC18.Thedifferencesintheselectivityofstationaryphasesofdifferentalkylchainlengthisalsoillustratedfortriphenylene,aplanarmoleculewithrestrictedrotationalmovement.TriphenyleneshowsmuchshorterretentiononC4relativetoC8andC18thanwouldbeexpectedonthebasisofhydrophobicityofthestationaryphase.Notethedifferenceinelutionorderfortriphenylenerelativetoimipramineandamylbenzeneforthismixtureonthisseriesofstationaryphases.
Different separation behaviors of Ph and ODS
13
2
4
10 200 min
YMC-Pack Ph
1
42
3
100 20 min
YMC-Pack ODS-AM DifferencesinretentionbehaviorofPhandODSforanalysisofwater-solublevitaminsareshownontheleft.TheelutionorderofriboflavinandcyanocobalaminonPhisthereverseofthatonODS.Thisisconsideredtobearesultofstrongretentionofcyanocobalaminduetoπ-πinteractioncausedbyphenylgroup.
1. p-Aminobenzoic acid (PABA)2. Biotin (Vitamin H)3. Riboflavin (Vitamin B2)4. Cyanocobalamin (Vitamin B12)
Column :150×4.6mmI.D.Eluent :acetonitrile/50mMNH4H2PO4(10/90)Flowrate :1.0mL/minTemperature:37℃Detection :UVat210nm,0.16AUFSInjection :10µL(0.02~ 0.30mg/mL)
第02章.indd 59 11.1.28 10:46:36 AM
60
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack Pro C8, C4● Superior separation of basic compounds● Processed with the same advanced
endcapping technology as Pro C18
● Excellent reproducibility● Utilizes highly pure silica gel base● Two types of test reports are included
with each column
H i g h l y e n d c a p p e d C8 a n d C4 r e v e r s e d - p h a s e c o l u m n sYMC-PackProC8andC4arehighlyappropriateforbasiccompoundssincemoreadvancedendcappingtechnologyisusedforprocessingoftheirresidualsilanolgroupsthatarelikelytoaffectquality.TheYMC-PackProC8andC4stationaryphasesurfacehydrophobicityislowerthanthatofODS,
makingYMC-PackProC8andC4usefulforquickanalysisofcompoundsthatdiffergreatlyinhydrophobicity.TheseparationbehaviorofhydrophiliccompoundsorplanarcompoundsonYMC-PackProC8andC4alsodiffersfromthatonODS,makingYMC-PackProC8andC4usefulforseparatingcompoundsincaseswhereseparationoptimizationisdifficulttoachieveusingODS.
C8■Poresize:120Å ■Carboncontent:10% ■UsablepHrange:2.0~ 7.5C4■Poresize:120Å ■Carboncontent:7% ■UsablepHrange:2.0~ 7.5
Separation of basic compounds
EffectiveendcappingofYMC-PackProC8andC4resultsingoodpeakshapesforbasiccompoundssuchasalkaloids.
Alkaloids
1. Scoporamine bromide2. Atropine sulfate3. Cinchonine4. Quinine5. Papaverine chloride
Shorten analysis time
Examplesofseparationofanalgesic/antipyreticdrugsonProC8,C4andC18areshownabove.Sinceibuprofenhasrelativelyhighhydrophobicity,muchtimeisrequiredtoanalyzeitusingthehighlyhydrophobicpackingmaterialProC18.WhenProC8orC4isused,thelowerhydrophobicityofthepackingmaterialcanshortentheanalysistime.Inaddition,ProC8andC4achievegoodseparationofhydrophiliccompounds,includingcaffeine,ethenzamideandbromovalerylurea.
Column :150×4.6mmI.D.Eluent :methanol/20mMH3PO4(60/40)Flowrate :1.0mL/minTemperature:37℃Detection :UVat254nm
Analgesic/Antipyretic drugs
1. Caffeine2. Ethenzamide3. Bromovalerylurea4. Ibuprofen
0 5 10 15 20 25 min
1 2
3
4
5
0 10 20 30 min 0 10 20 30 min0 10 20 30 min
1
2
3
4
1
2
3
41
2
3
4
Pro C18 Pro C8 Pro C4
0 10 20 30 min
1
2
3
4
Pro C8
0 10 20 30 min
1
2
3
4
Pro C4
Column :YMC-PackProC8(5µm,120Å) 150×4.6mmI.D.Eluent :acetonitrile/20mMKH2PO4(20/80)Flowrate :1.0mL/minTemperature:30℃Detection :UVat210nm
(A000303C)
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61
Reversed-P
hase Colum
ns (Other than O
DS
)
2
6 7
0 10 20 30 40 min
Buffer / MeOH (40/60)
1
2
34
5
Pro C8
0 10 20 min
Buffer / MeOH (40/60)
1
2
34
5
67
Pro C4
Optimization of separation using Pro C8 and Pro C4
RetentiontimesofanalytesonProC8andProC4tendtobeshorterthanthoseonC18.Whenalkylchainlengthsofpackingmaterialfunctionalgroupsareshorter,hydrogen-bondingcapacitiestendtobegreater;therefore,notonlyretentiontime,butalsoseparationselectivityofProC8andProC4maydifferfromthoseofC18.SeparationoptimizationisdifficulttoachieveforantiarrhythmicsusingProC18,evenifthemobilephaseischanged.Incontrast,C8andC4cancompletelyseparateantiarrhythmicsinashorttime.Asshownabove,C8andC4maybeusefulincaseswhereseparationoptimizationisdifficulttoachieveusingC18.
1. Phenytoin2. Propranolol HCl3. Quinidine4. Lidocaine5. Diltiazem HCl6. Verapamil HCl7. Nicardipine HCl
Column :150×4.6mmI.D.Eluent :20mMKH2PO4-K2HPO4(pH6.9)/methanolFlowrate :1.0mL/minTemperature:37℃Detection :UVat220nm
Separation of antiarrhythmics
0 10 20 30 min
1
2
3
4 5
6 7
Buffer / MeOH (35/65)Pro C18
0 10 20 30 40 50 min
1
2
3
45
6 7
Buffer / MeOH (40/60)Pro C18
Optimization of mobile phase
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62
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack Pro C8, C4
1
2
34
5
0 5 10 15 20 min
0 5 10 15 20 25 min
1
2
3
5
4
Pro C18
Pro C8
Pro C8
Column :
Eluent :
Flow rate :Temperature :Detection :
YMC-Pack Pro C8 and Pro C18150×4.6 mmI.D.20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (25/75)1.0 mL/min37 ℃UV at 254 nm
1.Toluene2.Deoxycorticosterone acetate3.Imipramine hydrochloride4.Triphenylene5.n-Amylbenzene
0 10 20min
0 10 20 30 40 min
12
34 5 6
21
4
3
5 6
Pro C18
Pro C4
Pro C4
Column :
Eluent :
Flow rate :Temperature :Detection :
YMC-Pack Pro C4 and Pro C18150×4.6 mmI.D.20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (35/65)1.0 mL/min37 ℃UV at 254 nm
1.Toluene2.Testosterone3.Acenaphthene4.n-Propylbenzene5.n-Butylbenzene6.Amitriptyline hydrochloride
Comparison of separation characteristics
ThesechromatogramsshowcomparisonoftheseparationcharacteristicsofProC8,ProC4andProC18.ProC8andProC4allowanalysisofcompoundswithgreatlydifferenthydrophobicityinashortertimethanProC18.Thesecolumnsalsoshowdifferentretentioncharacteristicsforhydrophilicorplanarcompoundsduetodifferentalkylchainlengthofthestationaryphases.Forsomegroupsofcompounds,ProC8andProC4mayprovidebetterseparationbehaviorinashortertime.
第02章.indd 62 11.1.28 10:46:43 AM
63
Reversed-P
hase Colum
ns (Other than O
DS
)
2
YMC-Pack Pro C8Analytical columns
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0× 35 OS12S03-H502WT2.0× 50 OS12S03-0502WT2.0× 75 OS12S03-L502WT2.0×100 OS12S03-1002WT2.0×150 OS12S03-1502WT3.0× 50 OS12S03-0503WT3.0×100 OS12S03-1003WT3.0×150 OS12S03-1503WT4.6× 35 OS12S03-H546WT4.6× 50 OS12S03-0546WT4.6× 75 OS12S03-L546WT4.6×100 OS12S03-1046WT4.6×150 OS12S03-1546WT
S-5 120 1.0×150 OS12S05-1501WT1.0×250 OS12S05-2501WT1.5×150 OS12S05-15P5WT1.5×250 OS12S05-25P5WT2.0× 50 OS12S05-0502WT2.0× 75 OS12S05-L502WT2.0×100 OS12S05-1002WT2.0×150 OS12S05-1502WT3.0× 50 OS12S05-0503WT3.0×150 OS12S05-1503WT3.0×250 OS12S05-2503WT4.6× 50 OS12S05-0546WT4.6× 75 OS12S05-L546WT4.6×100 OS12S05-1046WT4.6×150 OS12S05-1546WT4.6×250 OS12S05-2546WT6.0×150 OS12S05-1506WT
Semi-preparative columnsS-5 120 20×250 OS12S05-2520WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 OS12S03-0102CC
4.0×23 OS12S03-G304CC
S-5 120 1.0×10 OS12S05-0101CC1.5×10 OS12S05-01P5CC2.0×10 OS12S05-0102CC4.0×23 OS12S05-G304CC
Aguardcartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsS-5 120 20×50 OS12S05-0520WTG
YMC-Pack Pro C4Analytical columns
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0× 35 BS12S03-H502WT2.0× 50 BS12S03-0502WT2.0× 75 BS12S03-L502WT2.0×100 BS12S03-1002WT2.0×150 BS12S03-1502WT3.0× 50 BS12S03-0503WT3.0×100 BS12S03-1003WT3.0×150 BS12S03-1503WT4.6× 35 BS12S03-H546WT4.6× 50 BS12S03-0546WT4.6× 75 BS12S03-L546WT4.6×100 BS12S03-1046WT4.6×150 BS12S03-1546WT
S-5 120 1.0×150 BS12S05-1501WT1.0×250 BS12S05-2501WT1.5×150 BS12S05-15P5WT1.5×250 BS12S05-25P5WT2.0× 50 BS12S05-0502WT2.0× 75 BS12S05-L502WT2.0×100 BS12S05-1002WT2.0×150 BS12S05-1502WT3.0× 50 BS12S05-0503WT3.0×150 BS12S05-1503WT3.0×250 BS12S05-2503WT4.6× 50 BS12S05-0546WT4.6× 75 BS12S05-L546WT4.6×100 BS12S05-1046WT4.6×150 BS12S05-1546WT4.6×250 BS12S05-2546WT6.0×150 BS12S05-1506WT
Semi-preparative columnsS-5 120 20×250 BS12S05-2520WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 BS12S03-0102CC
4.0×23 BS12S03-G304CC
S-5 120 1.0×10 BS12S05-0101CC1.5×10 BS12S05-01P5CC2.0×10 BS12S05-0102CC4.0×23 BS12S05-G304CC
Aguardcartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsS-5 120 20×50 BS12S05-0520WTG
Ordering information
第02章.indd 63 11.1.28 10:46:44 AM
64
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack C8
● Stationary phase with lower hydrophobicity than ODS● Useful for separating samples with relatively high hydrophobicity
R e v e r s e d - p h a s e c o l u m n w i t h m o d e r a t e h y d r o p h o b i c i t yThehydrophobicityofYMC-PackC8ismoderateforareversed-phasepackingmaterial.RetentiontimesofsamplesonYMC-PackC8tendtobeshorterthanthose
onODSstationaryphase.ThemoderatehydrophobicityofYMC-PackC8makesitusefulforseparatingsampleswithrelativelyhighhydrophobicity.
■Poresize:120Å,200Å,300Å■Carboncontent:10% ,7% ,4%■UsablepHrange:2.0~ 7.5
10 20
21
3
4
0 min
Anti-HIV nucleoside derivatives
HN
O
O
N
NN
HOH C2
ddI
N
O
O
NH
N
HOH C2
2
ddC
HN
O
O
O
N
HOH C2
CH3
d4T
HN
O
O
O
N
HOH C2
CH3
3
AZT
N
1. 3. 4.2.
Column :YMC-PackC8(5µm,120Å) 150×4.6mmI.D.Eluent :methanol/10mMKH2PO4(10/60)Flowrate :1.0mL/minTemperature:37℃Detection :UVat254nm
A p p l i c a t i o n (K930311A)
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0× 35 OC12S03-H502WT2.0× 50 OC12S03-0502WT2.0× 75 OC12S03-L502WT2.0×100 OC12S03-1002WT2.0×150 OC12S03-1502WT3.0× 50 OC12S03-0503WT3.0×100 OC12S03-1003WT3.0×150 OC12S03-1503WT4.6×100 OC12S03-1046WT4.6×150 OC12S03-1546WT
S-5 120 1.0×150 OC12S05-1501WT1.0×250 OC12S05-2501WT1.5×150 OC12S05-15P5WT1.5×250 OC12S05-25P5WT2.0×150 OC12S05-1502WT2.0×250 OC12S05-2502WT4.6× 75 OC12S05-L546WT4.6×100 OC12S05-1046WT4.6×150 OC12S05-1546WT4.6×250 OC12S05-2546WT4.6×300 OC12S05-3046WT6.0×100 OC12S05-1006WT6.0×150 OC12S05-1506WT6.0×250 OC12S05-2506WT6.0×300 OC12S05-3006WT
200 4.6×150 OC20S05-1546WT4.6×250 OC20S05-2546WT
300 1.0×150 OC30S05-1501WT1.0×250 OC30S05-2501WT
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 300 1.5×150 OC30S05-15P5WT1.5×250 OC30S05-25P5WT2.0×150 OC30S05-1502WT2.0×250 OC30S05-2502WT4.6× 75 OC30S05-L546WT4.6×100 OC30S05-1046WT4.6×150 OC30S05-1546WT4.6×250 OC30S05-2546WT4.6×300 OC30S05-3046WT6.0×100 OC30S05-1006WT6.0×150 OC30S05-1506WT6.0×250 OC30S05-2506WT6.0×300 OC30S05-3006WT
S-10 120 4.6×100 OC12S11-1046WT4.6×150 OC12S11-1546WT4.6×250 OC12S11-2546WT4.6×300 OC12S11-3046WT6.0×100 OC12S11-1006WT6.0×150 OC12S11-1506WT6.0×250 OC12S11-2506WT6.0×300 OC12S11-3006WT
300 4.6×100 OC30S11-1046WT4.6×150 OC30S11-1546WT4.6×250 OC30S11-2546WT4.6×300 OC30S11-3046WT6.0×100 OC30S11-1006WT6.0×150 OC30S11-1506WT6.0×250 OC30S11-2506WT6.0×300 OC30S11-3006WT
Ordering information
第02章.indd 64 11.1.28 10:46:46 AM
65
Reversed-P
hase Colum
ns (Other than O
DS
)
2
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×150 OC12S05-1510WT10×250 OC12S05-2510WT10×300 OC12S05-3010WT20×100 OC12S05-1020WT20×150 OC12S05-1520WT20×250 OC12S05-2520WT
200 20×150 OC20S05-1520WT20×250 OC20S05-2520WT
300 10×150 OC30S05-1510WT10×250 OC30S05-2510WT10×300 OC30S05-3010WT20×150 OC30S05-1520WT20×250 OC30S05-2520WT
S-10 120 10×150 OC12S11-1510WT10×250 OC12S11-2510WT10×300 OC12S11-3010WT20×150 OC12S11-1520WT20×250 OC12S11-2520WT
200 20×150 OC20S11-1520WT20×250 OC20S11-2520WT
300 10×150 OC30S11-1510WT10×250 OC30S11-2510WT10×300 OC30S11-3010WT20×150 OC30S11-1520WT20×250 OC30S11-2520WT
SeeP148forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 OC12S03-0102CC
4.0×23 OC12S03-G304CC
S-5 120 1.0×10 OC12S05-0101CC1.5×10 OC12S05-01P5CC2.0×10 OC12S05-0102CC4.0×23 OC12S05-G304CC
200 4.0×23 OC20S05-G304CC
300 1.0×10 OC30S05-0101CC1.5×10 OC30S05-01P5CC2.0×10 OC30S05-0102CC4.0×23 OC30S05-G304CC
Aguardcartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×10 OC12S05-0104WFG5.0×10 OC12S05-0105WFG10×30 OC12S05-0310WTG20×50 OC12S05-0520WTG
200 4.0×10 OC20S05-0104WFG20×50 OC20S05-0520WTG
300 4.0×10 OC30S05-0104WFG5.0×10 OC30S05-0105WFG10×30 OC30S05-0310WTG20×50 OC30S05-0520WTG
S-10 120 4.0×10 OC12S11-0104WFG5.0×10 OC12S11-0105WFG10×30 OC12S11-0310WTG20×50 OC12S11-0520WTG
200 20×50 OC20S11-0520WTG
300 4.0×10 OC30S11-0104WFG5.0×10 OC30S11-0105WFG10×30 OC30S11-0310WTG20×50 OC30S11-0520WTG
Ordering information
第02章.indd 65 11.1.28 10:46:47 AM
66
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack C4
● Stationary phase with low hydrophobicity● Different separation characteristics from ODS
R e v e r s e d - p h a s e c o l u m n w i t h s h o r t e r a l k y l c h a i nTheYMC-PackC4stationaryphasesurfacehydrophobicityislowerthanthatofbothODSandC8RetentiontimesofsamplesonYMC-PackC4thereforetendtobeshorterthanthoseonODSorC8.Separation
characteristicsofYMC-PackC4alsodifferfromthoseofODS.YMC-PackC4achievesbetterseparationthanODSforsometypesofsamples.
■Poresize:120Å,200Å,300Å■Carboncontent:7% ,5% ,3%■UsablepHrange:2.0~ 7.5
A p p l i c a t i o n (T920302A)
1
2
34
5
6
7
8910
11
12
50 10 15 20 25 min
2,4-DNPH derivatives of aldehydes and ketones
Column :YMC-PackC4(5µm,120Å) 150×4.6mmI.D.Eluent :A)tetrahydrofuran/water(10/90) B)acetonitrile 35%B(0-7min),35-65%B(7-18min,linear), 100%B(18-19min),35%B(19-35min)Flowrate :1.5mL/minTemperature:30℃Detection :UVat360nm
1. Formaldehyde 2,4-DNPH 2. Acetaldehyde 2,4-DNPH 3. Acetone 2,4-DNPH 4. Acrolein 2,4-DNPH 5. Propionaldehyde, 2,4-DNPH 6. Crotonaldehyde 2,4-DNPH 7. Methylethylketone 2,4-DNPH 8. Isobutyraldehyde 2,4-DNPH 9. Benzaldehyde 2,4-DNPH 10. n-Valeraldehyde 2,4-DNPH 11. p-Tolualdehyde 2,4-DNPH 12. Capronaldehyde 2,4-DNPH
Ordering information
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0× 35 BU12S03-H502WT2.0× 50 BU12S03-0502WT2.0× 75 BU12S03-L502WT2.0×100 BU12S03-1002WT2.0×150 BU12S03-1502WT3.0× 50 BU12S03-0503WT3.0×100 BU12S03-1003WT3.0×150 BU12S03-1503WT4.6×100 BU12S03-1046WT4.6×150 BU12S03-1546WT
S-5 120 1.0×150 BU12S05-1501WT1.0×250 BU12S05-2501WT1.5×150 BU12S05-15P5WT1.5×250 BU12S05-25P5WT2.0×150 BU12S05-1502WT 2.0×250 BU12S05-2502WT4.6× 75 BU12S05-L546WT4.6×100 BU12S05-1046WT4.6×150 BU12S05-1546WT4.6×250 BU12S05-2546WT4.6×300 BU12S05-3046WT6.0×100 BU12S05-1006WT6.0×150 BU12S05-1506WT6.0×250 BU12S05-2506WT6.0×300 BU12S05-3006WT
200 4.6×150 BU20S05-1546WT4.6×250 BU20S05-2546WT
300 1.0×150 BU30S05-1501WT1.0×250 BU30S05-2501WT
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 300 1.5×150 BU30S05-15P5WT1.5×250 BU30S05-25P5WT2.0×150 BU30S05-1502WT2.0×250 BU30S05-2502WT4.6× 75 BU30S05-L546WT4.6×100 BU30S05-1046WT4.6×150 BU30S05-1546WT4.6×250 BU30S05-2546WT4.6×300 BU30S05-3046WT6.0×100 BU30S05-1006WT6.0×150 BU30S05-1506WT6.0×250 BU30S05-2506WT6.0×300 BU30S05-3006WT
S-10 120 4.6×100 BU12S11-1046WT4.6×150 BU12S11-1546WT4.6×250 BU12S11-2546WT4.6×300 BU12S11-3046WT6.0×100 BU12S11-1006WT6.0×150 BU12S11-1506WT6.0×250 BU12S11-2506WT6.0×300 BU12S11-3006WT
300 4.6×100 BU30S11-1046WT4.6×150 BU30S11-1546WT4.6×250 BU30S11-2546WT4.6×300 BU30S11-3046WT6.0×100 BU30S11-1006WT6.0×150 BU30S11-1506WT6.0×250 BU30S11-2506WT6.0×300 BU30S11-3006WT
第02章.indd 66 11.1.28 10:46:48 AM
67
Reversed-P
hase Colum
ns (Other than O
DS
)
2
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×150 BU12S05-1510WT10×250 BU12S05-2510WT10×300 BU12S05-3010WT20×100 BU12S05-1020WT20×150 BU12S05-1520WT20×250 BU12S05-2520WT
200 20×150 BU20S05-1520WT20×250 BU20S05-2520WT
300 10×150 BU30S05-1510WT10×250 BU30S05-2510WT10×300 BU30S05-3010WT20×150 BU30S05-1520WT20×250 BU30S05-2520WT
S-10 120 10×150 BU12S11-1510WT10×250 BU12S11-2510WT10×300 BU12S11-3010WT20×150 BU12S11-1520WT20×250 BU12S11-2520WT
200 20×150 BU20S11-1520WT20×250 BU20S11-2520WT
300 10×150 BU30S11-1510WT10×250 BU30S11-2510WT10×300 BU30S11-3010WT20×150 BU30S11-1520WT20×250 BU30S11-2520WT
SeeP149forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 BU12S03-0102CC
4.0×23 BU12S03-G304CC
S-5 120 1.0×10 BU12S05-0101CC1.5×10 BU12S05-01P5CC2.0×10 BU12S05-0102CC4.0×23 BU12S05-G304CC
200 4.0×23 BU20S05-G304CC
300 1.0×10 BU30S05-0101CC1.5×10 BU30S05-01P5CC2.0×10 BU30S05-0102CC4.0×23 BU30S05-G304CC
Aguardcartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×10 BU12S05-0104WFG5.0×10 BU12S05-0105WFG10×30 BU12S05-0310WTG20×50 BU12S05-0520WTG
200 4.0×10 BU20S05-0104WFG5.0×10 BU20S05-0105WFG20×50 BU20S05-0520WTG
300 4.0×10 BU30S05-0104WFG5.0×10 BU30S05-0105WFG10×30 BU30S05-0310WTG20×50 BU30S05-0520WTG
S-10 120 4.0×10 BU12S11-0104WFG5.0×10 BU12S11-0105WFG10×30 BU12S11-0310WTG20×50 BU12S11-0520WTG
200 20×50 BU20S11-0520WTG
300 4.0×10 BU30S11-0104WFG5.0×10 BU30S11-0105WFG10×30 BU30S11-0310WTG20×50 BU30S11-0520WTG
Ordering information
第02章.indd 67 11.1.28 10:46:49 AM
68
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack TMS● Stationary phase with the lowest hydrophobicity among reversed-phase packing
materials● Different separation characteristics from ODS
R e v e r s e d - p h a s e c o l u m n w i t h t h e l o w e s t h y d r o p h o b i c i t yYMC-PackTMSshowslowerretentionduetohydrophobicinteractionthanotherpackingmaterials,anditisusefulforelutinghighlyhydrophobiccompounds
inashorttime.Inaddition,itcansometimesachievegreaterretentionandbetterseparationofhydrophiliccompoundsthanotherreversed-phasecolumns.
■Poresize:120Å■Carboncontent:4%■UsablepHrange:2.0~ 7.5
Shorten analysis time using TMS
Column :50×2.0mmI.D.Eluent A)water/formicacid (100/0.05) B)acetonitrile/water/ formicacid(50/50/0.05)Flowrate :0.2mL/minTemperature:37℃Detection :ESIpositivemode
YMC-Pack Pro C18
Isocratic 36%B
5 10 15 20 25 30 min0
1
2
3
4
Gradient 30-90%B (0-10 min), 90%B (10-15 min)
5 10 15 min0
12
3
4
YMC-Pack Pro C18
YMC-Pack TMS
Isocratic 30%B
5 10 15 20 min0
1
2
3
4
Soy isoflavone
TMSenablesanalysistimeofhighlyhydrophobiccompoundstoshorten
1
2 34
100 20 min
Water-soluble vitamins1. p-Aminobenzoic acid (PABA)2. Biotin (Vitamin H)3. Riboflavin (Vitamin B2)4. Cyanocobalamin (Vitamin B12)
Column :YMC-PackTMS(5µm,120Å) 150×4.6mmI.D.
Eluent :acetonitrile/50mMNH4H2PO4(10/90)Flowrate :1.0mL/minTemperature:37℃Detection :UVat210nm
change column
change elution conditions
1.Daidzin2.Genistin3.Daidzein4.Genistein
A p p l i c a t i o n (M920212C)
第02章.indd 68 11.1.28 10:46:51 AM
69
Reversed-P
hase Colum
ns (Other than O
DS
)
2
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 4.6×100 TM12S03-1046WT4.6×150 TM12S03-1546WT
S-5 120 1.0×150 TM12S05-1501WT1.0×250 TM12S05-2501WT1.5×150 TM12S05-15P5WT1.5×250 TM12S05-25P5WT2.0×150 TM12S05-1502WT2.0×250 TM12S05-2502WT4.6× 75 TM12S05-L546WT4.6×100 TM12S05-1046WT4.6×150 TM12S05-1546WT4.6×250 TM12S05-2546WT4.6×300 TM12S05-3046WT6.0×100 TM12S05-1006WT6.0×150 TM12S05-1506WT6.0×250 TM12S05-2506WT6.0×300 TM12S05-3006WT
S-10 120 4.6×100 TM12S11-1046WT4.6×150 TM12S11-1546WT4.6×250 TM12S11-2546WT4.6×300 TM12S11-3046WT6.0×100 TM12S11-1006WT6.0×150 TM12S11-1506WT6.0×250 TM12S11-2506WT6.0×300 TM12S11-3006WT
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×150 TM12S05-1510WT10×250 TM12S05-2510WT10×300 TM12S05-3010WT20×100 TM12S05-1020WT20×150 TM12S05-1520WT20×250 TM12S05-2520WT
S-10 120 10×150 TM12S11-1510WT10×250 TM12S11-2510WT10×300 TM12S11-3010WT20×150 TM12S11-1520WT20×250 TM12S11-2520WT
SeeP150forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 4.0×23 TM12S03-G304CC
S-5 120 1.0×10 TM12S05-0101CC1.5×10 TM12S05-01P5CC2.0×10 TM12S05-0102CC4.0×23 TM12S05-G304CC
Aguardcartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×10 TM12S05-0104WFG5.0×10 TM12S05-0105WFG10×30 TM12S05-0310WTG20×50 TM12S05-0520WTG
S-10 120 4.0×10 TM12S11-0104WFG5.0×10 TM12S11-0105WFG10×30 TM12S11-0310WTG20×50 TM12S11-0520WTG
Ordering information
第02章.indd 69 11.1.28 10:46:52 AM
70
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack Ph● Reversed-phase column with π electrons● Unique selectivity due to π-π interaction● Useful in cases where separation optimization is difficult to achieve using ODS
D i f f e r e n t s e l e c t i v i t y f r o m O D SYMC-PackPhhasπelectronsofphenylgroup.YMC-PackPhshowsdifferentseparationcharacteristicsfromalkyl-silicastationaryphasesincludingODSforseparationofsolutessuchasaromaticcompounds,
sinceπ-πinteractionbetweenthestationaryphaseandsolutes,aswellashydrophobicinteraction,contributetotheseparation.
■Poresize:120Å■Carboncontent:9%■UsablepHrange:2.0~ 7.5
1. (-)-Epigallocatechin2. (+)-Catechin3. (-)-Epicatechin4. (-)-Epigallocatechin gallate5. (-)-Epicatechin gallate
Establishment of simple conditions using Ph
Column :150×4.6mmI.D.Flowrate :1.0mL/minTemperature:37℃Detection :UVat280nm
acetonitrile / water / TFA (12/88/0.1)
YMC-Pack Pro C18
1
3
5
2 4
N050622A
0 10 20 min
acetonitrile / ethyl acetate / water / TFA (9/3/88/0.1)
YMC-Pack Pro C18
2
34
5
1
N050620A
0 10 20 min
YMC-Pack Ph
acetonitrile / water / TFA (12/88/0.1)
2
53
1
4
N050623A
0 10 20 min
Catechins
Phissuitableforseparatingcatechinswithsimplesolvent,whereasifusingODSandoptimizinganalysisconditions,thesolventcomposition,additionwithaceticether,iscomplicated.
1
2
3
50 10 min
Aspartame in a commercially available carbonated beverage1. Benzoic acid2. Caffeine3. Aspartame
Column :YMC-PackPh(5µm,120Å) 150×4.6mmI.D.
Eluent :50mMKH2PO4/acetonitrile/methanol(84/3/13)Flowrate :1.5mL/minTemperature:30℃Detection :UVat214nm
change column
change solvent
A p p l i c a t i o n (T910123)
第02章.indd 70 11.1.28 10:46:55 AM
71
Reversed-P
hase Colum
ns (Other than O
DS
)
2
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0× 35 PH12S03-H502WT2.0× 50 PH12S03-0502WT2.0× 75 PH12S03-L502WT2.0×100 PH12S03-1002WT2.0×150 PH12S03-1502WT3.0× 50 PH12S03-0503WT3.0×100 PH12S03-1003WT3.0×150 PH12S03-1503WT4.6×100 PH12S03-1046WT4.6×150 PH12S03-1546WT
S-5 120 1.0×150 PH12S05-1501WT1.0×250 PH12S05-2501WT1.5×150 PH12S05-15P5WT1.5×250 PH12S05-25P5WT2.0×150 PH12S05-1502WT2.0×250 PH12S05-2502WT4.6× 75 PH12S05-L546WT4.6×100 PH12S05-1046WT4.6×150 PH12S05-1546WT4.6×250 PH12S05-2546WT4.6×300 PH12S05-3046WT6.0×100 PH12S05-1006WT6.0×150 PH12S05-1506WT6.0×250 PH12S05-2506WT6.0×300 PH12S05-3006WT
S-10 120 4.6×100 PH12S11-1046WT4.6×150 PH12S11-1546WT4.6×250 PH12S11-2546WT4.6×300 PH12S11-3046WT6.0×100 PH12S11-1006WT6.0×150 PH12S11-1506WT6.0×250 PH12S11-2506WT6.0×300 PH12S11-3006WT
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×150 PH12S05-1510WT10×250 PH12S05-2510WT10×300 PH12S05-3010WT20×100 PH12S05-1020WT20×150 PH12S05-1520WT20×250 PH12S05-2520WT
S-10 120 10×150 PH12S11-1510WT10×250 PH12S11-2510WT10×300 PH12S11-3010WT20×150 PH12S11-1520WT20×250 PH12S11-2520WT
SeeP150forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0×10 PH12S03-0102CC4.0×23 PH12S03-G304CC
S-5 120 1.0×10 PH12S05-0101CC1.5×10 PH12S05-01P5CC2.0×10 PH12S05-0102CC4.0×23 PH12S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×10 PH12S05-0104WFG5.0×10 PH12S05-0105WFG10×30 PH12S05-0310WTG20×50 PH12S05-0520WTG
S-10 120 4.0×10 PH12S11-0104WFG5.0×10 PH12S11-0105WFG10×30 PH12S11-0310WTG20×50 PH12S11-0520WTG
Ordering information
第02章.indd 71 11.1.28 10:46:56 AM
72
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack CN● Normal-phase and reversed-phase modes are selectable according to the purpose
of analysis● Low hydrophobicity● Unique selectivity due to cyano group
Column can be used in both normal -phase and reversed -phase modesYMC-PackCNcanbeusedinbothnormal-phaseandreversed-phasemodes,sinceithascyanopropylgroupofmediumpolaritychemicallybondedtothestationaryphase.Itcanbeusedinnormalphasemodewithlow-polaritymobilephasesuchashexane.Itcanalsobeusedinreversed-phasemodewithhighly-polarmobilephasesuchasmethanolandwater.
ThehydrophobicityofYMC-PackCNisrelativelylowforareversed-phasepackingmaterial,anditshowsdifferentselectivityfromODSduetoπelectronsofthecyanogroups.YMC-PackCNisusefulforshorteninganalysistimewhenretentiontimeistoolongwithODSandusefulincaseswhereseparationoptimizationisdifficulttoachieveusingODS.
■Poresize:120Å,300Å■Carboncontent:7% ,3%■UsablepHrange:2.0~ 7.5
10 20 min
1
4
32
0
Anti-hyperlipidemia drugs
CI O C COOHCONHCH CH2
CH
CH3
3
2
1.
Bezafibrate
CI O HC COOC
CH CH
CHCH3
3
2.
Clofibrate
52 O ( CH ) C COOH
CH
CH3
33
3
3.
Gemfibrozil
2 3
CI CO O C COOCH
CH
CH
CH
CH3
3
3
3
4.
Fenofibrate
Column :YMC-PackCN(5µm,120Å) 150×4.6mmI.D.
Eluent :acetonitrile/methanol/water/aceticacid(20/20/60/1)Flowrate :1.0mL/minTemperature:37℃Detection :UVat275nm
A p p l i c a t i o n (S931025E)
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 120 2.0× 35 CN12S03-H502WT2.0× 50 CN12S03-0502WT2.0× 75 CN12S03-L502WT2.0×100 CN12S03-1002WT2.0×150 CN12S03-1502WT3.0× 50 CN12S03-0503WT3.0×100 CN12S03-1003WT3.0×150 CN12S03-1503WT4.6×100 CN12S03-1046WT4.6×150 CN12S03-1546WT
S-5 120 1.0×150 CN12S05-1501WT1.0×250 CN12S05-2501WT1.5×150 CN12S05-15P5WT1.5×250 CN12S05-25P5WT2.0×150 CN12S05-1502WT2.0×250 CN12S05-2502WT4.6× 75 CN12S05-L546WT4.6×100 CN12S05-1046WT4.6×150 CN12S05-1546WT4.6×250 CN12S05-2546WT4.6×300 CN12S05-3046WT6.0×100 CN12S05-1006WT6.0×150 CN12S05-1506WT6.0×250 CN12S05-2506WT6.0×300 CN12S05-3006WT
Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 300 1.0×150 CN30S05-1501WT1.0×250 CN30S05-2501WT1.5×150 CN30S05-15P5WT1.5×250 CN30S05-25P5WT2.0×150 CN30S05-1502WT2.0×250 CN30S05-2502WT4.6× 75 CN30S05-L546WT4.6×100 CN30S05-1046WT4.6×150 CN30S05-1546WT4.6×250 CN30S05-2546WT4.6×300 CN30S05-3046WT6.0×100 CN30S05-1006WT6.0×150 CN30S05-1506WT6.0×250 CN30S05-2506WT6.0×300 CN30S05-3006WT
S-10 120 4.6×100 CN12S11-1046WT4.6×150 CN12S11-1546WT4.6×250 CN12S11-2546WT4.6×300 CN12S11-3046WT6.0×100 CN12S11-1006WT6.0×150 CN12S11-1506WT6.0×250 CN12S11-2506WT6.0×300 CN12S11-3006WT
Ordering information
第02章.indd 72 11.1.28 10:46:58 AM
73
Reversed-P
hase Colum
ns (Other than O
DS
)
2
Semi-preparative columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 10×150 CN12S05-1510WT10×250 CN12S05-2510WT10×300 CN12S05-3010WT20×100 CN12S05-1020WT20×150 CN12S05-1520WT20×250 CN12S05-2520WT
300 20×150 CN30S05-1520WT20×250 CN30S05-2520WT
S-10 120 10×150 CN12S11-1510WT10×250 CN12S11-2510WT10×300 CN12S11-3010WT20×150 CN12S11-1520WT20×250 CN12S11-2520WT
SeeP151forpreparativecolumnsotherthanthoselistedabove.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 CN12S03-0102CC
4.0×23 CN12S03-G304CC
S-5 120 1.0×10 CN12S05-0101CC1.5×10 CN12S05-01P5CC2.0×10 CN12S05-0102CC4.0×23 CN12S05-G304CC
300 1.0×10 CN30S05-0101CC1.5×10 CN30S05-01P5CC2.0×10 CN30S05-0102CC4.0×23 CN30S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-5 120 4.0×10 CN12S05-0104WFG5.0×10 CN12S05-0105WFG10×30 CN12S05-0310WTG20×50 CN12S05-0520WTG
300 4.0×10 CN30S05-0104WFG5.0×10 CN30S05-0105WFG10×30 CN30S05-0310WTG20×50 CN30S05-0520WTG
S-10 120 4.0×10 CN12S11-0104WFG5.0×10 CN12S11-0105WFG10×30 CN12S11-0310WTG20×50 CN12S11-0520WTG
Ordering information
第02章.indd 73 11.1.28 10:46:58 AM
74
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMC-Pack PROTEIN-RP● Improved recovery of proteins or peptides● Improved durability when used with TFA solution● Enables elution of high molecular weight proteins
R e v e r s e d - p h a s e c o l u m n f o r s e p a r a t i o n o f p r o t e i n s o r p e p t i d e sYMC-PackPROTEIN-RPisareversed-phasecolumnutilizingasilicagelbase.Itcontainsastationaryphase,specificallydesignedforseparationofproteinsorpeptides.Problemsthatareassociatedwithconventional
reversed-phasecolumnswithshortalkylchainlengthsareminimized.Robustcolumnlifetimeandexcellentrecoveryofhydrophobicproteinsaretypicallypossiblewiththisphase.
■Carboncontent:4%■UsablepHrange:1.5~ 7.5
Improved recovery of proteins or peptides
Recoveryofvariousproteinsandpeptidesareshown.YMC-PackPROTEIN-RPgivesgreaterrecoverythancompetitorC4columns.
recovery of proteins or peptides
SampleRecovery(%)
YMC-Pack PROTEIN-RP
C4 column,company A
C4 column,company B
Ribonuclease A 93 95 86Cytochrome c 94 89 95Lysozyme 98 93 76Myoglobin 97 85 93BSA 94 92 86Ovalbumin 90 73 88Transferrin 94 98 88Insulin(bovine) 97 73 92Insulin B chain 82 76 70α-Mating factor 93 82 81Leu-Enkephalin 92 84 91Gly-Gly-Gly-Gly 95 86 70
Improved durability when used with TFA solution
110
100
90
80
70
60
50
40
30
20
10
0100 200 300 400
C4 column, Company B
C4 column, Company A
(Peak split)
YMC-Pack PROTEIN-RP
×
Solvent flow in hours
0.1%TFA condition
% retention of diphenyl
1. Uracil2. Benzene3. Naphthalene4. Diphenyl
〈Flowconditions〉Eluent :water/TFA(100/0.1)Flowrate :1.0mL/minTemperature:ambient
〈Measurementconditions〉Column :YMC-PackPROTEIN-RP
250×4.6mmI.D.Eluent :acetonitrile/water(40/60)Flowrate :1.0mL/minTemperature:30℃Detection :UVat254nm,0.32AUFS
Testresultsofthestabilityofstationaryphasewith0.1%aqueousTFAisshownontheleft.RetentionofdiphenylonC4columnsmanufacturedbyothercompaniesgreatlydecreasesastimepasses.Thisiscausedbycleavageofbutylgroupsfromthepackingmaterialduetoacidhydrolysis.RetentionofdiphenylonYMC-PackPROTEIN-RPisshowntobestableafter500hoursofmobilephaseflow.
〈Prior to flow〉1
2
3
4
0 5 10 min
Theoretical plate number (4) = 14,300
〈After 500 hours〉
1
2
3
4
0 5 10 min
Theoretical plate number (4) = 13,800
第02章.indd 74 11.1.28 10:47:01 AM
75
Reversed-P
hase Colum
ns (Other than O
DS
)
2
0 10 20 30 min
12
3
Proteins1. Cytochrome c (MW:12,400, 5 µg)2. BSA (MW:66,000, 25 µg)3. Ferritin (MW:440,000, 10 µg)
Column :YMC-PackPROTEIN-RP250×4.6mmI.D.
Eluent :A)water/TFA(100/0.1) B)acetonitrile/TFA(100/0.1) 30-90%B(0-45min,linear)
Flowrate :1.0mL/minTemperature:ambientDetection :UVat280nm,0.04AUFS
A p p l i c a t i o n
1
23 4 5
67
8
9
1011
12
13
403020100 min
Proteins and peptides
Column :YMC-PackPROTEIN-RP 250×4.6mmI.D.
Eluent :A)water/TFA(100/0.1) B)acetonitrile/TFA(100/0.1) 10-90%B(0-60min,linear)
Flowrate :1.0mL/minTemperature:ambientDetection :UVat220nm,0.32AUFS
1. Met-Enkephalin2. Leu-Enkephalin3. Oxytocin4. Bradykinin5. Angiotensin l6. Ribonuclease A7. α-Mating factor
8. Insulin(bovine)9. Cytochrome c
10. Lysozyme11. BSA12. β-Lactoglobulin13. Ovalbumin
A p p l i c a t i o n
Analytical columnsParticle size(µm)
Column sizeinnerdiameter×length(mm) Product number
S-5 2.0×150 PR99S05-1502WT2.0×250 PR99S05-2502WT4.6×150 PR99S05-1546WT4.6×250 PR99S05-2546WT
Semi-preparative columnsS-5 10×250 PR99S05-2510WT
20×150 PR99S05-1520WT20×250 PR99S05-2520WT
Guard cartridge columns (three-pack)S-5 4.0×23 PR99S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderCartridge holder(inner diameter: 4.0 mm)
XPCHW
Guard columnsParticle size(µm)
Column sizeinnerdiameter×length(mm) Product number
S-5 4.0×10 PR99S05-0104WFG10×30 PR99S05-0310WTG20×50 PR99S05-0520WTG
Ordering information
第02章.indd 75 11.1.28 10:47:02 AM
76
2
Reversed-P
hase Colum
ns (Other than O
DS
)
Analytical columns
YMCbasic● Superior separation of basic compounds● Useful for separation of peptides● Secondary interaction minimized as much as possible
C o l u m n f o r s e p a r a t i o n o f b a s i c c o m p o u n d sYMCbasicisareversed-phasesilicabasedC8columndesignedforseparationofbasiccompounds,includingpharmaceuticalproducts.Itishighlyevaluatedasa"neutral"silanolgroupinEuropeandtheU.S.Itoffers
superiorseparationofacidiccompoundsaswellasbasiccompounds.Itissuitableforseparatingpeptideswithmolecularweightsintherangeofseveralthousands,suchasinsulin.
■Carboncontent:7%■UsablepHrange:2.0~ 7.5
0 10 20 30 40 50
0
20
40
60mAU
60 min
*
* undigested BSA
Tryptic digest of BSA
Column :YMCbasic(5µm) 150×2.0mmI.D.
Eluent :A)water/TFA(100/0.1) B)acetonitrile/TFA(100/0.1) 5-35% B(0-50min),35-45% B(50-55min), 45% B(55-60min)Flowrate :0.2mL/minTemperature:37℃Detection :UVat220nm
A p p l i c a t i o n
Ordering information
Analytical columnsParticle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 200 2.0× 35 BA99S03-H502WT2.0× 50 BA99S03-0502WT2.0× 75 BA99S03-L502WT2.0×100 BA99S03-1002WT2.0×150 BA99S03-1502WT3.0× 50 BA99S03-0503WT3.0×100 BA99S03-1003WT3.0×150 BA99S03-1503WT4.6× 50 BA99S03-0546WT4.6×100 BA99S03-1046WT4.6×150 BA99S03-1546WT
S-5 200 2.0×150 BA99S05-1502WT3.0×150 BA99S05-1503WT4.6× 50 BA99S05-0546WT4.6×100 BA99S05-1046WT4.6×150 BA99S05-1546WT4.6×250 BA99S05-2546WT6.0×150 BA99S05-1506WT6.0×250 BA99S05-2506WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)Particle size(µm)
Pore size(Å)
Column sizeinnerdiameter×length(mm) Product number
S-3 200 4.0×23 BA99S03-G304CC
S-5 200 2.0×10 BA99S05-0102CC4.0×23 BA99S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)
XPCHSMW
Cartridge holder(inner diameter: 4.0 mm)
XPCHW
第02章.indd 76 11.1.28 10:47:03 AM
Reversed-P
hase Colum
ns (Other than O
DS
)
2
77
Analytical columns
YMC Carotenoid● Resolves polar and nonpolar geometric carotenoid isomers● Separates carotenoids in blood samples, food products, natural product extracts, and
commercial preparations● Operates with low aqueous or non aqueous mobile phases desirable in LC/MS and prep fraction recovery
C a r o t e n o i d a n a l y t i c a l c o l u m nYMCCarotenoidisC30bondedsilicabasedreversedphasecolumn.Itisforcarotenoidanalysisandusefulforseparationofgeometricisomers.
■UsablepHrange:2.0~ 7.5
0 10 20 30 40 50 60 70 80
1
23
4
5
6
7
8
9
10
11
12
13 14
min
Carotene and xanthophyll
Column :YMCCarotenoid 250×4.6mmI.D.
Eluent :A)methanol/MTBE*/H2O(81/15/4)B)methanol/MTBE*/H2O(6/90/4)0-100%B(0-90min)
Flowrate :1.0mL/minTemperature:ambientDetection :UVat450nm*methyltert-butylether
1. Astaxanthin2. Capsanthin3. Lutein4. Zeaxanthin5. Canthaxanthin6. β-Cryptoxanthin7. Echinenone8. 15-cis β-Carotene9. 13-cis β-Carotene
10. α-Carotene11. trans β-Carotene12. 9-cis β-Carotene13. δ-Carotene14. Lycopene
A p p l i c a t i o n
Ordering information
Analytical and Semi-preparative columnsParticle size(µm)
Column sizeinnerdiameter×length(mm) Product number
S-3 4.6×100 CT99S03-1046WT4.6×150 CT99S03-1546WT
S-5 4.6×150 CT99S05-1546WT4.6×250 CT99S05-2546WT20×150 CT99S05-1520WT20×250 CT99S05-2520WT
Guard cartridge columns (three-pack)Particle size(µm)
Column sizeinnerdiameter×length(mm) Product number
S-3 4.0×23 CT99S03-G304CC
S-5 4.0×23 CT99S05-G304CC
Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.
Cartridge holderCartridge holder(inner diameter: 4.0 mm)
XPCHW
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第02章.indd 78 11.1.28 10:47:05 AM