Protea’s 5,000 sq ft laboratory features BIOiTRAQ MALDI TOF/TOF (ABI 4800) and LTQ (Thermo) mass spectrometers
Isolate - Fractionate - Collect - Digest - Separate - Analyze
BIOLOGY clinical samples: ü blood ü tissue ü urine ü sputum
CHEMISTRY
Sample Analysis by
Mass Spec
Connecting Biology to Mass Spectrometry
Connecting Biology to Mass Spectrometry a new product offering
Products for Gel-Based Proteomics and Genomics
Products for Solution-Based Proteomics
Products for Capillary Electrophoresis
Services
Isolate - Fractionate - Collect - Digest - Separate - Analyze
BIOLOGY clinical samples: ü blood ü tissue ü urine ü sputum
CHEMISTRY
Sample Analysis by
Mass Spec
The GPR Gel Protein Recovery System
Ø Recovers intact proteins from gels
Ø Removes salts, detergents and dyes
Ø Improves sample quality for downstream MS
Ø Enables top-down proteomics
Ø Elutes proteins from 5kDa to 200 kDa
Ø Quantitative protein recovery data available
Products for Gel-Based Proteomics and Genomics
Related Gel-Based Proteomics Products
SDSaway detergent removal
Silver Staining Kit
Protein MW Markers
Solvates SDS detergent from the protein surface Fast, simple - works
Ultrafast Ultrasensitive (20-40ng/band)
Designed for use with Laemmli SDS-PAGE gels From 6,500 to 200,000 Da
Products for Gel-Based Proteomics and Genomics
Programmable Syringe Pump
Ø Fully programmable
Ø Operates standalone or from a computer
Accessories for Sample Preparation
ProteaGeltm Products
Ø Ready to use
Ø No stacking gel required = longer gel separations
Ø Stable at room temperature for one year
Ø Mass Spec Certified
Products for Gel-Based Proteomics and Genomics
SeaClear Agarose products
Ø Complete Line of agarose products
Ø Value Pricing
Ø Protea in-house Quality Testing
Products for Gel-Based Proteomics and Genomics
Proteases and ProteaBeadstm
Protea Endoproteinases Ø Certified Mass Spec Grade Ø Performance Tested
ProteaBeads Ø Immobilized Trypsin & Glu-C
Ø Rapid, efficient digestion
Ø Prevents contamination of sample with autodigestion products
Ø Available in flow-through cartridges
Products for Solution-Based Proteomics
MALDI sample prep
Complete kit - optimizes samples for MALDI MS Removes Coomasie Stain bound to protein Uses ultrapure matrix
Products for Solution-Based Proteomics
Figure 1 presents the effect of coomasie stain on the MALDI-TOF MS signal of a Myoglobin protein sample. The spectrum reveals different number of coomasie molecules bound to the protein molecules as indicated by the resolved adducts in the spectrum. This adduction of coomasie to protein molecules results in reduced sensitivity since the protein signal is spread over the different adducts. In addition, the coomasie-protein adducts can mask the presence of any post-translationally modified protein molecules. Removal of coomasie by destaining therefore results in an improved MS signal
UltraPure MALDI Matrices
Protea Ultrapure CHCA matrix Vs. competitor
Ø Improved MALDI-MS sensitivity
Ø Forms homogenous crystals
Ø Easy reconstitution
Products for Solution-Based Proteomics
Reversed Phase Sample Preparation Products
Ø C18 ProteaTips - effective for salt removal
Ø Clean-up of peptides from in-gel digested proteins
Ø C18 ProteaPlate - 96 well sample cleanup
C4 & C8 Tips also available Supplied as kits with buffers and protocols
Products for Solution-Based Proteomics
Titanium Dioxide Sample Preparation Products
For enrichment of phosphopeptides in protein digest samples, particularly complex protein/ peptide samples Provided as complete kits with sample reconstitution and wash buffers Also available in 96 well plates
Products for Solution-Based Proteomics
ZICtm HILIC ProteaTips & Sample Prep Kit
Effective in removal of detergents & salts Enriches peptides complementary To those trapped by C18 ZipTips Low backpressure during Sample processing
Products for Solution-Based Proteomics
Zic is a registered trade mark of Sequant AB
Facettm Columns for ESI Mass Spectrometry
Made of PEEK Biocompatible plastic Trapping Columns Now Available:
Reversed Phase (RP) Cation Exchange (CX) ZICtm -HILIC
Also available:
FACET microfilters
Products for Solution-Based Proteomics
Zic is a registered trade mark of Sequant AB
Products for Capillary Surface Modification
Ø Capillary Coating Kit
Ø Reproducible control of EOF
Ø Stable for wide pH ranges
Ø Coatings resistant to irreversible Protein adsorption
Products for Capillary Electrophoresis
Plastic Chip Design & Fabrication Services
Features Plastic microfluidic chips 100 micron features Short turnaround times Applications Capillary electrophoresis Reaction monitoring Micro-sample prep
Services
Berkely.edu
Protein Research Services
Ø BIOiTRAQ capability
Ø Use of our new products, eg TiO2
Ø Gel-based sample capabilities
Ø De Novo Sequencing (PEAKS studio)
Services
www.appliedbiosystems
Protein Standards
Products for Solution-Based Proteomics
Mark of Quality Assurance
Isolate - Fractionate - Collect - Digest - Separate - Analyze
gel protein recovery protein digestion protein separation Disposable micro-cartridges packed with protein separation matrices:
hydrophobic hydrophilic reverse phase ion exchange
Connecting Biology to Proteomics