JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 2 * 1975
EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief(1979)
Centerfor Disease Control,Atlanta, Ga.
LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,
New Orleans, La.
NATHALIE J. SCHMIDT, Editor (1975)California Department ofHealth,
Berkeley, Calif.
HENRY D. ISENBERG, Editor(1979)Long IslandJewish-Hillside Medical Center,
New Hyde Park, N.Y.
JOHN A. WASHINGTON II, Editor(1978)Mayo Clinic
Rochester, Minn.
Daniel Amsterdam (1977)Libero Ajello (1976)Hugo L. David(1975)Walter R. Dowdle (1976)V. R. Dowell, Jr. ( 1975)Richard W. Emmons (1977)John C. Feeley (1977)James D. Fenters ( 1976)Sydney M. Finegold (1976)Maxwell Finland (1975)Tbomas L. Gavan (1977)Morris A. Gordon (1975)Dieter Growchel (1977)James L. Hardy (1975)Melvin T. Hatch (1975)Lillian V. Holdeman (1976)
S. S. Kalter (1975)Leo Kaufman (1977)George E. Kenny (1976)Morton Klein (1975)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)William J. Martin (1975)John M. Matsen (1977)Joseph L. Melnick (1976)William 1. Metzger (1977)Maurice A. Mufson (1977)Demosthenes Pappagianis ( 1977)Robert H. Purcell (1977)William E. Rawls(1977)
Jon E. Rosenblatt( 1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1975)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton 1. Taylor (1977)John H. Thompson, Jr. (1977)Marvin Turck ( 1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1975)Ronald A. Ward (1977)Robert E. Weaver (1975)Robert P. Williams (I1978)Marion E. Wilson( 1977)
Robert A. Day, Managing EditorGisella Pollock, Director ofEditorial Services
Leslie Frymire, Production Editor1913 1St., N. W., Washington, D.C. 20006
EX OFFICIOHelen R. Whiteley, President (1975-1976)
J. Mehsen Joseph, Secretary
The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N.W., Washing-ton, D.C. 20006, is devoted to the dissemination of new knowl-edge concerning the applied microbiological aspects of humanand animal infections and infestations, particularly regardingtheir etiologic agents, diagnosis, and epidemiology. Papersdealing with antibiotics and antimicrobial agents and chemo-therapy, with fundamental aspects of infection and immunity.and with food or dairy microbiology fall within the scope ofother ASM publications. The Journal is published monthly,
Harlyn 0. Halvorson, Vice-President (1975-1976)Brinton M. Miller, Treasurer
and the twelve numbers are divided into two volumes per year.The nonmember subscription price is $50 per year. The mem-ber subscription price is $12 per year. Single copies are $5.Correspondence relating to subscription, reprints, defectivecopies, availability of back issues, lost or late proofs, dispo-sition of submitted manuscripts. and general editorial mattersshould be directed to the ASM Publications Office. 1913 1 St..N.W., Washington, D.C. 20006 (area 202 833-9416).
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Washington, D.C.Made in the United States of America.Copyright 0 1975, American Society for Microbiology.1975, American Society for Microbiology.All Rights Reserved.
Author IndexAllen, Richard, 524Anderson, Roger L., 486Anhalt, John P., 510
Bennett, John V., 486Berger, J., 541Bredt, W., 541
Carter, Philip L., 474Chow, Anthony W., 546Crandell, Robert A., 465
Dee, Thomas H., 482Deidrick, Velma R., 478Dowdle, W. R., 531
Edberg, Stephen C., 469
Flanigan, S. M., 559Flodstrom, Anders, 504
Gray, Ernest D., 528Greenwood, J. R., 559
Grossgebauer, K., 516Guze, Lticien B., 546
Hallander, Hans O., 504Hedlund, Kenneth, 524Holmberg, Kenneth, 504
Kerner, H., 516Kuwert, E., 516
Lam, W., 541
Mackel, Donald C., 486Maki, Dennis G., 486Martin, W. J., 559Melloh, Ann J., 465Mostow, S. R., 531
Novak, Marta, 469
Patten, Valerie, 546Pemberton, John R., 549Peters, J., 516
Pickett, M. J., 559Pohle, H. D., 516
Rhame, Frank S., 486Rubin, Sally Jo, 556Ryan, Terence J., 474
Schild, G. C., 531Singer, Jacques MI., 469Slater, Helen, 469Slechta, Thomas F., 528Sorlie, Pamela, 465Spicher, G., 516Sumaya, Ciro V., 520Symington, Dorothy A., 498
Tilton, Richard C., 556Toma, S., 478
von Riesen, V. Lyle, 552, 554
Wood, R. J., 531
Yu, Pauline K. W., 510
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ACKNOWLEDGMENTThe following have served as ad hoc reviewers from 11 September 1974 to 14 October 1975 and
their help is greatly appreciated.
H. E. AdlerDonald G. AhearnWilliam L. AlbrittonAaron D. AlexanderStephen D. AllenJohn P. Anhalt
Jack BaldwinR. A. BankowskiSamuel BaronArthur L. BarryJohn E. BennettDon J. BrennerSonja M. Buckley
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Announcing the definitive reference bookin clinical microbiology...
NEW SECOND EDITION OF Manual ofCLINICAL MICROBIOLOGY
Edited by EDWIN H. LENNETTE, Viral and Rickettsial Disease Laborat-ory, California Department of Health, Berkeley; EARLE H.SPAULDING,De-partment of Microbiology and Immunology, Temple University HealthSciences Center, Philadelphia; and JOSEPH P.TRUANT, Advance Medi-cal Laboratories, Pontiac, Michigan.
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AUTHOR INDEX
VOLUME 2
Abe, Takemaru, 89Adock, G. B., 206Adess, Michael, 382Ahearn, D. G., 354Allen, Richard, 524Altemeier, William A., 337Amstein, Charles F., 46Anderson, Roger L., 486Anhalt, John P., 510Ash, Ronald J., 270Ashley, C. R., 327Atherton, John M., 318
Bagley, Lauren R., 121Balcerzak-Raczkowski, I. B.,305
Balows, Albert, 235Banffer, J. R. J., 361Bannister, E. R., 149Barnhart, E. R., 270Bauman, David S., 77Beheshti, Firooz, 345Bennett, John V., 486Berger, J., 541Blank, F., 115Blazevic, Donna J., 266Blouse, Louis E., 318Bor, Eva, 226Bowman, P. I., 354Bredt, W., 541Brenner, Don J., 235Burdash, N. M., 149Burger, Gary T., 121Butler, Diane A., 322
Caie, Ian S., 153Calisher, Charles H., 198Caprioli, T., 311Carter, Philip L., 474Caul, E. O., 327Champion, Margaret L., 268Chappell, W., Adrian, 287Chow, Anthony W., 546Cosgrove, Jeanne O., 139Crandell, Robert A., 465Cummins, C. S., 104
Darland, Gary, 391Dee, Thomas H., 482Deidrick, Velma R., 478Dietel, John W., 430Delanerolle, V., 21Dick, C. R., 403Dick, E. C., 403Dilworth, John A., 453Dowdle, W. R., 531Driesens, Robert J., 85Drucker, D. B., 162
Duncan, R. C., 149Dyar, C., 149
Eckert, Herbert L., 42Edberg, Stephen C., 469Ederer, Grace M., 266Ehret, Josephine M., 142Eickhoff, Theodore C., 142Ellen, R. P., 305Emmons, Richard W., 81Eng, Sam, 67Eskenazy, M., 368Evans, James B., 14Everett, E. Dale, 142
Falcao, D. P., 349Farmer, J. J., III, 349, 359, 397Farrow, Wendell M., 419Faur, Yvonne C., 178Feldman, Harry A., 136Fink, Jordan N., 55Fischer, John B., 111Flanigan, S. M., 559Flodstrom, Anders, 504Forghani, Bagher, 410Foy, Hjordis, 226Frisch-Niggemeyer, W., 377Friz, Mary, 218Furukawa, Toru, 332
Gamble, William C., 287Gavan, Thomas L., 322Glosser, James W., 218Goldberg, I. D., 387Gordon, M. A., 74Goto, Hitoshi, 89Gray, Ernest D., 528Greenwood, J. R., 559Groschel, Dieter, 96Grossgebauer, K., 516Group$, Vincent, 419Guze, Lucien B., 546Gwaltney, Jack M., Jr., 453
Hahon, Nicholas, 42Hall, Marsha, 18Hall, Marsha, 296Hallander, Hans O., 504Hammar, Sherrel, 226Hanker, Jacob S., 463Harding, Sally A., 222Harkness, John L., 296Harper, Gloria, 21Hart, E. J., 387Hartman, Paul A., 46Hatch, Milford H., 231Hauser, K. J., 1Hedlund, Kenneth, 524
Hendley, J. Owen, 453Hickman, Frances W., 349Hickman, Robert, 226Hill, Edward O., 337Hiscott, Thomas, 186Ho, Helen H., 183Holdeman, Lillian V., 272Holland, Joseph W., 337Holmberg, Kenneth, 504Hopfer, Roy L., 96, 115Hopkins, J. M., 206Hornberger, Elizabeth, 332Horstmeier, Carlyle, 18Hubbard, Mary E., 186Hulst, A. M., 361Huppert, M., 21
Ilstrup, Duane M., 296Inhorn, S. L., 403Isenberg, Henry D., 99, 139Ivanova, R., 368
Jacobs, Norman F., Jr., 292Jarnagin, Jerald L., 268Jimenez-Ulate, Franklin, 441Jones, Lois F., 397Jones, Wallis L., 218Kalinova, R., 368Kanamitsu, Masatsugu, 89Kane, Judith A., 35Kane, Julius, IIIKarakawa, Walter K., 35Kasatiya, S. S., 311Kenny, George, 276Kenyon, Richard H., 121Kenyon, Richard H., 300Kerner, H., 516Kertesz, Vera, 461Klein, Dolph, 425Kolonel, Laurence, 318Kouroupis, G. M., 8Krause, George W., 345Kurup, Viswanath P., 55Kuwert, E., 516
Lagios, Michael D., 62Lam, W., 541Land, G. A., 206Larson, D., 253Lawman, Michael J. P., 153Lee, S. M., 162Lee, Wie-Shing, 62Leers, W. D., 8Legakis, N. J., 373, 459Lennette, David A., 81Lennette, Edwin H., 81, 183,410
Leonards, Richard, 62
ii AUTHOR INDEX
Lewis, Vester J., 193Lo, James W., 129Lobregat, Corazon M., 322Lunceford, C. D., 456Lund, Marlys E., 266
McGlynn, Doris M., 186McGonagle, Lee Anne, 67Mackel, Donald C., 486Maker, Myron D., 70Maki, Dennis G., 486Malinin, Theodore I., 243Malone, Beatrice H., 272Maness, Kathryn S. C., 198Mirrett, Stanley, 243Martin, R. S., 438Martin, W. J., 559Matsen, John M., 425Mayyasi, S., 253Melloh, Ann J., 465Merz, William G., 222Minor, T. E., 403Mirrett, Stanley, 247, 266Mitzel, J. R., 126Mizrahi, A., 11Monroe, Patrick W., 382Montes de Oca, Hector, 243Morgan, Patrick M., 382Mostow, S. R., 531Munch, K., 255
Norden, Carl W., 136Novak, Marta, 469
Osterman, Joseph V., 430
Page, L. A., 165Painter, Barbara G., 99Papavassiliou, J., 373, 459Patten, Valerie, 546Patterson, William R., 130Pedersen, Carl E., Jr., 121Pedersen, Carl E., Jr., 300Pemberton, John R., 144, 147,549
Peters, J., 516Pickett, M. J., 559Plotkin, Stanley A., 332Pohle, H. D., 516Probert, M., 157
Raahave, Dennis, 277
Rabin, Albert N., 463Rabkin, Elizabeth, 35Raghoenath, S., 361Reddick, Anne, 72Reller, L. Barth, 247, 266Rhame, Frank S., 486Richmond, Shirley J., 327Robart, E. M., 438Roberts, Glenn D., 18, 261Robertson, P. W., 461Rogers, Faye, 218Rubin, Sally Jo, 556Russell, S. M., 157Ryan, Terence J., 474Rytel, Michael W., 173
Sakuma, Sadatoshi, 332Salkin, I. F., 74Sammons, Lenora S., 121Sammons, Lenora St. C., 300Sande, Merle A., 453Scales, Roy W., 292Schelhart, Dorothy, 281Scherer, Jane S., 139Schild, G. C., 531Shimizu, Kiheiji, 89Schmidt, Gertrud, 67Schmidt, Nathalie J., 183, 410Schmitt, Martin W., 419Schneider, Nathan, J., 272Schreider, Minnie, 272Shayegani, Mehdi, 186Shepard, M. C., 456Shirai, Akira, 430Silberg, Stanley L., 382Simao, Emilia, 397Singer, Jacques M., 469Skaggs, Romulus, 292Slater, E. A., 126Slater, Helen, 469Slechta, Thomas F., 528Smith, Andrew G., 345Smith, Coy D., 77Smith, Kendall 0., 136Smith, Thomas F., 134Sol-Vernin, Carlos, 397Sorlie, Pamela, 465Sottile, Margaret I., 173Spicher, G., 516Spindler, Joyce A., 425Stalheim, 0. H. V., 165, 169Stauffer, Larry R., 337
Stephens, R., 253Stewart, John, 42Stewart, Paula, 453Strahilov, D., 368Strating, A., 126Stone, S. S., 169Sullivan, James D., Jr., 151Sulzer, Catherine R., 218Sumarah, R. K., 438Sumaya, Ciro V., 520Sun, Sung H., 21Symington, Dorothy A., 498
Taylor, Welton I., 281Tess, Bernard R., 129Thacker, W. Lanier, 193Thoen, Charles 0., 268Thomas, Evan T., 397Tilton, Richard C., 5,56Toma, S., 478Trabulsi, L. R., 349Traul, K., 253
Van Scoy, Robert E., 94Vinton, E. C., 206von Riesen, V. Lyle, 552, 554
Walter, Paul D., 193Washington, John A., II, 18, 70,
94, 296Watkins, Charles A., 318Watson, Stanley W., 151Weed, Lyle A., 134Weisburd, Martin H., 178West, M. E., 149White, Lendell A., 287Wilkins, Tracy D., 441Wilson, Marion E., 178Wilson, Walter R., 94Winkler, W. Gerald, 193Wirsing, Karla, 67Wolff, J., 253Wood, R. J., 531
Xilinas, M. E., 459
Yeager, Anne S., 448Yewdall, Russell C., 186Yu, Pauline K. W., 510
Zabranksy, R. J., 1Zaccour, F., 311
J. CLIN. MICROBIOL.
SUBJECT INDEX
VOLUME 2
Acetoin, 162Actinomyces nae8lundii, 305Actinomyces ViSCOsu8s, 305Agarose beads, 292Anaerobic bacteriaevaluation of new column packing material, 1modification of gas-liquid procedure, 1
Anaerobic growth of staphylococci, 14Anaerobic specimen transport device, 441Anaerobic strains of C. vaginale (H. vaginalis), 272Antibiotic susceptibility, 391Antibodyantinuclear determinations, 42influenza virus complement-fixing, 287B. canis, 382
Antigensformalinized bacterial, 359hepatitis B, 8, 231pneumococcal types VII and XIV, 510
Antinuclear antibody determinations, 42API 20E system, 322Arthritis
identification of M. agalactiae subsp. bovis from,169
of cattle, 165Aspergillus differential medium, 74A8pergillus fumigatus, 482Assay, plaque, 126Auxanographic method, 206
Bacilli, 149Bacteremia
detection of in culture bottles, 296incidence of in adults, 94
Bacteriaanaerobic, 1classification by direct inoculation procedure,469
recovery from peritonsillar abscesses, 504Bacterial identification, 391Bacterial phospholipases, 373Bactericidal test, 136Bacteriological sampling, 277Bacteroides, 337Batch adsorption, 528Blood culture bottles, 296Blood culture tube, 99Bovine group B streptococci, 35Bovine rhinotracheitis virus, 46.5Brucella canis antibodies, 382
Candida albicans, 345Carbohydrate fermentation test, 12Catalase-positive microorganisms, 463Chiamydia, 134Chlamydia trachomatis, 327Chlamydospore, 345Choriomeningitis virus infection, 193
iii
Chromatography, gas-liquid, 1, 162Classification of bacteria, 469Clinical isolation of Y. enterocolitica, 559Color reach on streak test, 463Complement fixation test, 77Congenital infection, 461Conjugates, 296Contamination of intravenous products, 486Corynebacterium vaginale, 272Counterimmunoelectrophoresiscomparison with Reiter protein, 361for subtyping hepatitis B antigen, 231of pneumococcal antigens, 510to detect A. fumigatus, 482to identify S. pneumoniae, 173
Coxsackieviruses (group A), 183Cryptococcus neoformans
identification of, 115identified by six-hour pigmentation test, 96
Culturesblood, bottle, 296blood, tube, 99human lymphocyte cell line, 11
Cytomegalovirus infections, 448
Dental plaque bacteria, 305Diacetyl, 162Differential medium, 305Dual-staining method, 149
Encise II, 139Enteric Analyzer
to identify Enterobacteriaceae, 186, 235Enteric pathogens, 281Enterobacteriaceae
identification by Enteric Analyzer, 186, 235identification of enteric pathogens, 247lactose-positive S. typhimurium, 349resembling E. coli, 70
Enterotube, 139Epidemiologic study, 520Equine influenza, 89Escherichia coli, 70
Fifty-milliliter vacutainer blood culture tube, 99Formaldehyde
retention of preservative levels, 144vapor, 516
Formalinized bacterial antigens, 359Four culture media, 438Fungi, 261Fusobacterium, 337
Gas-liquid chromatographyanalysis of acetoin and diacetyl, 162to identify anaerobic bacteria, 1
Genito-urinary tract, 178Glow discharge, 46
iv SUBJECT INDEX
Haemophilus influenzae, 136Hemagglutinationcompared with radioimmunoassay method, 8reversed passive, 8rubella virus, 524
Heparin, 151Hepatitis B antigenscomparison of methods for, 8counterelectrophoresis method, 231
Herpes simplex viral antibodies, 410Herpes simplex virus, 270Histoplasmosis, 77Human cytomegalovirus, 332Hypersensitivity pneumonitis, 55
Immunodiffusion test, 77Immunoelectrophoretic patterns, 129Immunofluorescence method, 81Immunofluorescence of yeast in urine, 142Immune fluorescence microscopy, 121Immunoglobulin coupled to agarose beads, 292Immunoglobulin G antibodies, 377Immunoglobulin M antibodies, 377Immunoglobulin G receptors, 332Indirect antibody technique, 153Indirect fluorescent antibody procedure, 337Indirect hemagglutination test
for diagnosis of human leptospirosis, 218for human antibody to typhus and rickettsiae,430
Indirect immunoferritin technique, 327Indole production, 425Infectionchoriomeningitis virus, 193cytomegalovirus, 448mumps virus, 81wound, 62, 556
Influenza A vaccine, 403Influenza type A virus, 531Influenza virus complement-fixing antibody, 287Intravenous products, 486
Klebsiella bacillus, 425
Japanese quail, 419
Leptospiral microscopy agglutination test, 474Leptospirosis, 218Less commonly used media, 554Leukocyte-transforming agent, 520Limulus test, 151Lymphocytic choriomeningitis virus infection, 193
Media table, 67Medium
Aspergillus differential, 74caffeic acid-containing, 115differential, 305motility-indole-lysine, 247motility-whole-lysine sulfide, 266
Mercurial preservatives in desiccated biologicalproducts, 549
Methodauxanographic, 206
counterelectrophoresis, 231dual-staining, 149hemagglutination, 8immunofluorescence, 81microscopy, 541serum-soft agar, 35
Microscopyimmune fluorescence, 121method to identify M. pneumoniac, 541phase-contrast, 261
Microtechnique, 474Modified fluorescent antibody technique, 461Motility-indole-lysine medium, 247Motility-indole-lysine-sulfide medium, 266Mouse ascitic fluid, 287Mumps virus infections, 81Mycobacterium paratuberculosis, 268Mycobacterium tuberculosis, 438Mycoplasma agalactiae subsp. bovis, 169Mycoplasma hominis, 226Mycoplasma pneumoniae, 541Mycoplasmal arthritis, 165
Neisseria, 72Neisseria gonorrhoeae
conditions required for colony-type stability of,387
improved transport system for, 498Neisseria meningitidis, 178
Oncornaviruses, 253
Parainfluenza-3 virus antibody, 157Peritonsillar abscesses, 504Phage typing scheme
for group D streptococci, 311S. epidermidis strains, 318
Phase-contrast microscopy, 261Phenol, 147Phospholipases, 373Photostatic copying of immunoelectrophoretic
patterns, 129Plaque assay of rabies virus, 126Pneumococcal antigens, 510Pneumococcimethods to improve detection of, 448, 453
Polyols, 11Polypectate digestion by Yersinia, 552Porcine kidney cell monolayers, 126Precipitin tests, 104Preparation of media, 554Procedure of gas-liquid chromatography, 1Propionibacterium acnes, 104Proteus, 459Prototheca wickerhamii, 62Pseudomonas aeruginosa, 397Pyruvate, 14
Quail cell cultures, 419
Radioimmunoassaycompared with hemagglutination method, 8for measurement of viral antibody, 130to determine antibody frequencies, 136
J. CLIN. MICROBIOL.
SUBJECT INDEX v
RD cells, 183Reiter protein, 361Renal cells, 243Respiratory secretions, 453Rickettsiae, 430Rickettsia rickettsii, 121Rocky Mountain spotted fever vaccines, 300Rubella infections, 377Rubella virus hemagglutination, 524
Saprophytic alga, 62Salmonella antisera, 368Salmonella lipopolysaccharides, 368Salmonella typhimuriumendemic in Brazil, 349unusual Enterobacteriaceae, 349
Seroagglutination test, 268Seroepidemiological investigation
of B. canis antibodies, 382on equine influenza, 89
Serum-soft agar method, 35Single radial hemolysis technique, 157Single radial diffusion test, 531Six-hour pigmentation test, 96Smallpox, 516Solid phase radioimmunoassay, 410Staphylococci, 14Staphylococcus epidermidis, 318Streptococcal nuclease B, 528Streptococcibovine group B, 35isolated from human urogenital tract, 311phage typing scheme, 311
Streptococcus pneumoniae, 173Syphilis, 361
Techniquecounterimmunoelectrophoresis, 173, 231, 361,
482, 510indirect antibody, 153indirect immunoferritin, 327single radial hemolysis, 157thin-layer chromatographic, 373
Temperature effect on media for enteric patho-gens, 281
Testbactericidal, 136carbohydrate fermentation, 72color reaction streak test, 463complement fixation, 77immunodiffusion, 77indirect hemagglutination, 218leptospiral microscopy agglutination, 474precipitin, 104radioimmunoassay, 136
seroagglutination, 268single radial diffusion, 531six-hour pigmentation, 96
Thermophilic actinomycetes associated with hy-persensity pneumonitis, 55
Thin-layer chromatographic technique, 373Tissue culture, 46Transport system for N. gonorrhoeae, 498Trichophyton megninii, 111Toxoplasma gondii, 461Typhoid vaccine, 85Typhus, 430
Ultraviolet fluorescence, 546Uni-Yeast-Tek kit, 354Uracil, 14Ureaplasma urealyticumprevalence in adolescents, 226unusual colonies of, 456
Urinary tract infection, 222Urogenital tract, 311
VaccineAlice, 403Rocky Mountain spotted fever, 300typhoid, 85
Velvet pads, 277Veillonella, 546Venezuelan equine encephalitis virus, 198Vibrio alginolyticus, 556Viral antibody, 130Virusbovine rhinotracheitis, 465choriomeningitis, 193herpes simplex, 270influenza type A, 531mumps, 81rabies, 126rubella, 524Venezuelan equine encephalitis, 198
Wound infectionby P. wickerhamii, 62isolation of V. alginolyticus, 556
Yeastsevaluation of antibody coating, 222identification by Uni-Yeast-Tek kit, 354immunofluorescence of, 142rapid methods to identify, 21recovery from blood culture bottles, 18
Yersinia, 552Yersinia enterocolitica
clinical isolation of, 559isolation from swine, 478
VOL. 2, 1975
INDEX TO DATE OF ISSUE
Month Date of Issue Pages
July 5 August 1975 1-75August 2 September 1975 77-151September 30 September 1975 153-276October 30 October 1975 277-360November 3 December 1975 361-464December 31 December 1975 465-560
JOURNALOF
CLINICAL
MICROBIOLOGY
VOLUME 2
WASHINGTON, D.C. 20006
1975
JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 2 *NUMBER 6* DECEMBER 1975
EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief(1979)
Centerfor Disease Control,Atlanta, Ga.
LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,
New Orleans, La.
NATHALIE J. SCHMIDT, Editor(1975)California Department ofHealth,
Berkeley, Calif.
HENRY D. ISENBERG, Editor(1979)Long Island Jewish-Hillside Medical Center,
New Hyde Park, N. Y.
JOHN A. WASHINGTON II, Editor(1978)Mayo Clinic
Rochester, Minn.
Daniel Amsterdam (1977)Libero Ajello (1976)Hugo L. David (1975)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1975)Richard W. Emmons (1977)John C. Feeley (1977)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1975)Thomas L. Gavan (1977)Morris A. Gordon (1975)Dieter Groschel (1977)James L. Hardy (1975)Melvin T. Hatch (1975)Lillian V. Holdeman (1976)
S. S. Kalter (1975)Leo Kaufman (1977)George E. Kenny (1976)Morton Klein (1975)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)William J. Martin (1975)John M. Matsen (1977)Joseph L. Melnick (1976)William I. Metzger (1977)Maurice A. Mufson (1977)Demosthenes Pappagianis (1977)Robert H. Purcell (1977)William E. Rawls (1977)
Jon E. Rosenblatt (1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1975)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton 1. Taylor (1977)John H. Thompson, Jr. (1977)Marvin Turck (1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1975)Ronald A. Ward (1977)Robert E. Weaver (1975)Robert P. Williams (1978)Marion E. Wilson (1977)
Robert A. Day, Managing EditorGisella Pollock, Director ofEditorial Services
Leslie Frymire, Production Editor1913 I St., N. W., Washington, D.C. 20006
EX OFFICIOHelen R. Whiteley, President (1975-1976)
J. Mehsen Joseph, Secretary
The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N.W., Washing-ton, D.C. 20006, is devoted to the dissemination of new knowl-edge concerning the applied microbiological aspects of humanand animal infections and infestations, particularly regardingtheir etiologic agents, diagnosis, and epidemiology. Papersdealing with antibiotics and antimicrobial agents and chemo-therapy, with fundamental aspects of infection and immunity,and with food or dairy microbiology fall within the scope ofother ASM publications. The Journal is published monthly,
Harlyn 0. Halvorson, Vice-President (1975-1976)Brinton M. Miller, Treasurer
and the twelve numbers are divided into two volumes per year.The nonmember subscription price is $50 per year. The mem-ber subscription price is $12 per year. Single copies are $5.Correspondence relating to subscription, reprints, defectivecopies, availability of back issues, lost or late proofs, dispo-sition of submitted manuscripts, and general editorial mattersshould be directed to the ASM Publications Office, 1913 I St.,N.W., Washington, D.C. 20006 (area 202 833-9416).
Application to mail at second-class postage rates is pending atWashington, D.C.Made in the United States of America.Copyright i 1975, American Society for Microbiology.1975, American Society for Microbiology.All Rights Reserved.
Volume 2 Contents for July Number 1Modification of the Gas-Liquid Chromatography Procedure and Evaluation of a
New Column Packing Material for the Identification of Anaerobic Bacteria.K. J. HAUSER* AND R. J. ZABRANSKY .1
Comparison of the Reversed Passive Hemagglutination with RadioimmunoassayMethods for Hepatitis B Antigen. W. D. LEERS* AND G. M. KOUROUPIS 8
Pluronic Polyols in Human Lymphocyte Cell Line Cultures. A. MIZRAHI .. 11Uracil and Pyruvate Requirements for Anaerobic Growth of Staphylococci. JAMES
B. EVANS.14
Recovery of Yeasts from Blood Culture Bottles. GLENN D. ROBERTS, CARLYLEHORSTMEIER, MARSHA HALL, AND JOHN A. WASHINGTON II* ..18
Rapid Methods for Identification of Yeasts. M. HUPPERT,* GLORIA HARPER, SUNGH. SUN, AND V. DELANEROLLE.21
Demonstration of the Capsular Antigens of Bovine Group B Streptococci by theSerum-Soft Agar Method. JUDITH A. KANE,* ELIZABETH RABKIN, AND WALTERW. KARAKAWA.35
Evaluation of Cellular Substrates for Antinuclear Antibody Determinations.NICHOLAS HAHON,* HERBERT L. ECKERT, AND JOHN STEWART.42
Adaptation of Plastic Surfaces for Tissue Culture by Glow Discharge. CHARLESF. AMSTEIN AND PAUL A. HARTMAN*.46
A Scheme for the Identification of Thermophilic Actinomycetes Associated withHypersensitivity Pneumonitis. VISWANATH P. KURUP* AND JORDAN N. FINK .. 55
Wound Infection by Prototheca wickerhamii, a Saprophytic Alga Pathogenic forMan. WIE-SHING LEE,* MICHAEL D. LAGIOS, AND RICHARD LEONARDS.62
A Space-Saving Media Table. LEE ANNE MCGONAGLE,* GERTRUD SCHMIDT, SAMENG, AND KARLA WIRSING.67
Unclassified, Lactose-Fermentating, Urease-Producing Member of the FamilyEnterobacteriaceae Resembling Escherichia coli. JOHN A. WASHINGTON II*AND MYRON D. MAKER.70
A Simple Carbohydrate Fermentation Test for Identification of the PathogenicNeisseria. ANNE REDDICK.72
Evaluation of Aspergillis Differential Medium. I. F. SALKIN* AND M. A. GORDON .. 74
Volume 2 Contents for August Number 2Comparison of Immunodiffusion and Complement Fixation Tests in the Diagnosis
of Histoplasmosis. DAVID S. BAUMAN* AND CoY D. SMITH.77Rapid Diagnosis of Mumps Virus Infections by Immunofluorescence Methods.
DAVID A. LENNETTE, RICHARD W. EMMONS, AND EDWIN H. LENNETTE*.81Effect of Glass on pH-Dependent Stability of Typhoid Vaccine. ROBERT J.DRIESENS.85
Sero-Epidemiological Study on Equine Influenza in Japan. HrrOSHI GOTO,*KIHEIJI SHIMIZU, TAKEMARU ABE, AND MASATSUGU KANAMITSU.89
Incidence of Bacteremia in Adults Without Infection. WALTER R. WILSON, * ROBERTE. VAN SCOY, AND JOHN A. WASHINGTONII.94
Six-Hour Pigmentation Test for the Identification of Cryptococcus neoformans.RoY L. HOPFER* AND DIETERGROSCHEL.96
Clinical Laboratory Evaluation of the Fifty-Milliliter Vacutainer Blood Culture*Asterisk refers to person to whom inquiries regarding the paper should be addressed.
ix
Tube. BARBARA G. PAINTER* AND HENRY D. ISENBERG.99Identification of Propionibacterium acnes and Related Organisms by Precipitin
Tests with Trichloroacetic Acid Extracts. C. S. CUMMINS.104Occurrence of Trichophyton megninii in Ontario. Identification with A Simple
Cultural Procedure. JULIUS KANE AND JOHN B. FISCHER* ..i..l....l....illCaffeic Acid-Containing Medium for Identification of Cryptococcus neoformans.
Roy L. HOPFER* AND F. BLANK.115Demonstration of Rickettsia rickettsii in the Rhesus Monkey by Immune Fluores-
cence Microscopy. CARL E. PEDERSEN, JR.,* LAUREN R. BAGLEY, RICHARD H.KENYON, LENORA S. SAMMONS, AND GARY T. BURGER.121
Plaque Assay of Rabies Virus on Porcine Kidney Cell Monolayers. A. STRATING,*J. R. MITZEL, AND E. A. SLATER.126
Photostatic Copying of Immunoelectrophoretic Patterns with the Use of ColorPrint Filters. JAMES W. Lo* AND BERNARD R. TESS.129
Improvements of a Radioimmunoassay for Measurement of Viral Antibody inHuman Sera. WILLIAM R. PATrERSON* AND KENDALL 0. SMITH.130
Comparison of Urethral Swabs, Urine, and Urinary Sediment for the Isolation ofChlamydia. THOMAS F. SMITH AND LYLE A. WEED.134
Haemophilus influenzae, Type b, Antibody Frequencies Determined with Bacteri-cidal and Radioimmunoassay Tests. CARL W. NORDEN* AND HARRY A.FELDMAN.136
Clinical Laboratory Evaluation of the Further Improved Enterotube and EnciseII. HENRY D. ISENBERG,* JANE S. SCHERER, AND JEANNE COSGROVE......... 139
Immunofluorescence of Yeast in Urine. E. DALE EVERETT,* THEODORE C. EICKHOFF,AND JOSEPHINE M. EHRET.142
Retention of Preservative Levels of Formaldehyde in Dessiccated BiologicalProducts. JOHN R. PEMBERTON.144
Retention of Preservative Levels of Phenol in Desiccated Biological Products.JOHN R. PEMBERTON.147
Evaluation of a Dual-Staining Method for Acid-Fast Bacilli. N. M. BURDASH,*M. E. WEST, E. R. BANNISTER, C. DYAR, AND R. C. DUNCAN.149
Inhibitory Effect of Heparin on the Limulus Test for Endotoxin. JAMES D. SULLI-VAN, JR.,* AND STANLEY W. WATSON.151
Volume2 Contents for September Number3Design and Construction of an Apparatus for the Growth of Micro Cell Cultures
on Standard Glass Microscope Slides and Its Application for Screening LargeNumbers of Sera by the Indirect Antibody Technique. MICHAEL J. P. LAW-MAN* AND IAN S. CAIE.153
Measurement of Parainfluenza-3 Virus Antibody by the Single Radial HemolysisTechnique. M. PROBERT* AND S. M. RUSSELL.157
Analysis of Acetoin and Diacetyl in Bacterial Culture Supernatants by Gas-Liq-uid Chromatography. S. M. LEE AND D. B. DRUCKER*.162
Naturally Occurring and Experimentally Induced Mycoplasmal Arthritis of Cattle.0. H. V. STALHEIM* AND L. A. PAGE.165
Isolation and Identification of Mycoplasma agalactiae subsp. bovis from ArthriticCattle in Iowa and Nebraska. 0. H. V. STALHEIM* AND S. S. STONE.169
x CONTENTS
Application of Counterimmunoelectrophoresis in the Identification of Streptococcuspneumoniae in Clinical Isolates. MARGARET I. SOTTILE* AND MICHAEL W.RYTEL.173
Isolation of Neisseria meningitidis from the Genito-Urinary Tract and Anal Canal.YVONNE C. FAUR,* MARTIN H. WEISBURD, AND MARION E. WILSON.178
Propagation and Isolation of Group A Coxsackieviruses in RD Cells. NATHALIE J.SCHMIDT, HELEN H. Ho, AND EDWIN H. LENNETTE*.183
Evaluation of the Enteric Analyzer for Identification of Enterobacteriaceae.MEHDI SHAYEGANI,* MARY E. HUBBARD, THOMAS HISCOTT, DORIS M. McGLYNN,AND RUSSELL C. YEWDALL.186
Comparison of Three Tests for Serological Diagnosis of Lymphocytic Choriomen-ingitis Virus Infection. VESTER J. LEWIS, * PAUL D. WALTER, W. LANIERTHACKER, AND W. GERALD WINKLER.193
Laboratory Studies of Venezuelan Equine Encephalitis Virus in Equines, Texas,1971. CHARLES H. CALISHER* AND KATHRYN S. C. MANESS.198
Improved Auxanographic Method for Yeast Assimilations: a Comparison withOther Approaches. G. A. LAND, * E. C. VINTON, G. B. ADCOCK, AND J. M.HOPKINS.206
Evaluation of an Indirect Hemagglutination Test for the Diagnosis of HumanLeptospirosis. CATHERINE R. SULZER,* JAMES W. GLOSSER, FAYE ROGERS,WALLIS L. JONES, AND MARY FRIZ.218
Evaluation of Antibody Coating of Yeasts in Urine as an Indicator of the Siteof Urinary Tract Infection. SALLY A. HARDING AND WILLIAM G. MERZ* .... 222
Prevalence of Mycoplasma hominis and Ureaplasma urealyticum (T Strains) inUrine of Adolescents. HJORDIS FOY,* GEORGE KENNY, EVA BOR, SHERRELHAMMAR, AND ROBERT HICKMAN.226
Modified Counterelectrophoresis Method for Subtyping Hepatitis B Antigen.MILFORD H. HATCH.231
Evaluation of the Enteric Analyzer, an Instrument to Aid in the Identification ofEnterobacteriaceae. DON J. BRENNER* AND ALBERT BALOWS.235
Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys.HEcrOR MONTES DE OCA AND THEODORE I. MALININ*.243
Motility-Indole-Lysine Medium for Presumptive Identification of Enteric Path-ogens of Enterobacteriaceae. L. BARTH RELLER* AND STANLEY MIRRETT .... 247
New Method for the Removal of Extraneous Proteins from Purified Oncornaviruses.K. TRAUL,* D. LARSON, R. STEPHENS, J. WOLFF, K. MUNCH, AND S. MAYYASI ... 253
Detection of Fungi in Clinical Specimens by Phase-Contrast Microscopy. GLENND.ROBERTS.261
Motility-Indole-Lysine-Sulfide Medium. GRACE M. EDERER,* MARLYS E. LUND,DONNA J. BLAZEVIC, L. BARTH RELLER, AND STANLEY MIRRETT.266
Seroagglutination Test for Identification of Mycobacterium paratuberculosis.JERALD L. JARNAGIN,* MARGARET L. CHAMPION, AND CHARLES 0. THOEN ...... 268
Optimal Cooling and Warming Rates in the Preservation of Herpes Simplex Virus(Type 2). RONALD J. ASH* AND E. R. BARNHART.270
Obligately Anaerobic Strains of Corynebacterium vaginale (Haemophilus vaginalis).BEATRICE H. MALONE, MINNIE SCHREIBER, NATHAN J. SCHNEIDER, AND LILLIAN V.HOLDEMAN.272
ERRATUMRapid Methods for Identification of Yeasts. H. HUPPERT,* GLORIA HARPER, SUNG
H. SUN, AND V. DELANEROLLE.276
XiCONTENTS
Volume2 Contents for October Number4New Technique for Quantitative Bacteriological Sampling of Wounds by Velvet
Pads: Clinical Sampling Trial. DENNIS RAAHAVE.277Effect of Temperature on Transport and Plating Media for Enteric Pathogens.
WELTON I. TAYLOR* AND DOROTHY SCHELHART.281Production of Influenza Virus Complement-Fixing Antibody in Mouse Ascetic
Fluid. LENDELL A. WHITE,* WILLIAM C. GAMBLE, AND W. ADRIAN CHAPPELL .. .. 287
Use of Immunoglobulin Coupled to Agarose Beads for Examining the Specificity ofConjugates. Roy W. SCALES,* NORMAN F. JACOBS, JR., AND ROMULUS SKAGGS .. 292
Effects of Atmosphere of Incubation and of Routine Subcultures on Detection ofBacteremia in Vacuum Blood Culture Bottles. JOHN L. HARKNESS, MARSHAHALL, DUANE M. ILSTRUP, AND JOHN A. WASHINGTON II*.296
Comparison of Three Rocky Mountain Spotted Fever Vaccines. RICHARD H.KENYON,* LENORA ST. C. SAMMONS, AND CARL E. PEDERSEN,JR. 300
Differential Medium for Detecting Dental Plaque Bacteria Resembling Acti-nomyces viscosus and Actinomyces naeslundii. R. P. ELLEN* AND I. B.BALCERZAK-RACZKOWSKI.305
Phage Typing Scheme for Group D Streptococci Isolated from Human UrogenitalTract. T. CAPRIOLI, F. ZACCOUR, AND S. S. KASATIYA*.311
Efficacy of Phage Typing Epidemiologically Related Staphylococcus epidermidisStrains. LOUIS E. BLOUSE,* LAURENCE N. KOLONEL, CHARLES A. WATKINS, ANDJOHN M. ATHERTON .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .;.. .. .. .. .. 318
Reproducibility of the Analytab (API 20E) System. DIANE A. BUTLER, CORAZONM. LOBREGAT, AND THOMAS L. GAVAN*.322
Identification of the Elementary Bodies of Chlamydia trachomatis in the ElectronMicroscope by an Indirect Immunoferritin Technique. C. R. ASHLEY,SHIRLEY J. RICHMOND, * AND E. 0. CAUL.327
Demonstration of Immunoglobulin G Receptors Induced by Human Cytomega-lovirus. TORU FURUKAWA,* ELIZABETH HORNBERGER, SADATOSHI SAKUMA, ANDSTANLEY A. PLOTKIN.332
Indirect Fluorescent Antibody Procedure for the Rapid Detection and Identifica-tion of Bacteroides and Fusobacterium in Clinical Specimens. LARRY R.STAUFFER,* EDWARD 0. HILL, JOSEPH W. HOLLAND, AND WILLIAM A. ALTEMEIER . 337
Germ Tube and Chlamydospore Formation by Candida albicans on a New Medium.FIROOz BEHESHTI, ANDREW G. SMITH,* AND GEORGE W. KRAUSE.345
Unusual Enterobacteriaceae: Lactose-Positive Salmonella typhimurium Which isEndemic in Sao Paulo, Brazil. D. P. FALCAO, L. R. TRABULSI, FRANCES W.HICKMAN, AND J. J. FARMER III* 349
Evaluation of the Uni-Yeast-Tek Kit for the Identification of Medically ImportantYeasts. P. I. BOWMAN AND D. G. AHEARN*.354
Formalinized Bacterial "Antigens" as a Potential Infection Hazard. J. J.FARMER III.359
Volume 2 Contents for November Number 5Comparison of the Counter-Immunoelectrophoresis Technique with the Reiter
Protein and Three Other Serological Tests as First Line Test for Syphilis.J. R. J. BXNFFER,* S. RAGHOENATH, AND A. M. HULST.361
xii CONTENTS
Insolubilized Salmonella Lipopolysaccharides as Efficient Immunoadsorbents forthe Preparation of Specific Salmonella Antisera. M. ESKENAZY,* D. STRAHILOV,R. IVANOVA, AND R.KALINOVA.368
Thin-Layer Chromatographic Technique for Rapid Detection of' Bacterial Phos-pholipases. N. J. LEGAKIS* AND J. PAPAVASSILIOU.373
Rapid Separation of Immunoglobulin M from Immunoglobulin G Antibodies for Re-liable Diagnosis of Recent Rubella Infections. W. FRISCH-NIGGEMEYER . 377
Seroepidemiological Investigation of Brucella canis Antibodies in Different HumanPopulation Groups. PATRICK W. MONROE,* STANLEY L. SILBERG, PATRICK M.MORGAN, AND MICHAELADESS.382
Conditions Required for the Attainment of Colony-Type Stability of' Neisseriagonorrhoeae in Liquid Culture. E. J. HART* AND I. D. GOLDBERG .387
Discriminant Analysis of Antibiotic Susceptibility as a Means of Bacterial Identifi-cation. GARYDARLAND.391
Epidemiology of Pseudomonas aeruginosa in a General Hospital: a Four-YearStudy. EVAN T. THOMAS,* Lois F. JONES, EMILIA SIMAO, CARLOS SOLE'-VERNIN,AND J. J. FARMERIII.397
Attenuated Influenza A Vaccine (Alice) in an Adult Population: Vaccine-RelatedIllness, Serum and Nasal Antibody Production, and Intrafamily Transmission.T. E. MINoR, E. C. DICK,* C. R. DICK, AND S. L.INHORN.403
Solid Phase Radioimmunoassay for Typing Herpes Simplex Viral Antibodies inHuman Sera. BAGHER FORGHANI, NATHALIE J. SCHMIDT, AND EDWIN H. LEN-NETTE .410
Responses of Isolator-Derived Japanese Quail and Quail Cell Cultures in SelectedAnimal Viruses. WENDELL M. FARROW,* MARTIN W. SCHMITr, AND VINCENTGROUP'E.419
Relationship of Indole Production and Antibiotic Susceptibility in the Kiebsiellabacillus. DOLPH KLEIN, JoycE A. SPINDLER, AND JOHN M. MATSEN* ......425
Indirect Hemagglutination Test for Human Antibody to Typhus and Spotted FeverGroup Rickettsiae. AKIRA SHIRAI, JOHN W. DIETEL, AND JOSEPH V. OSTERMAN* 430
Comparison of Four Culture Media for the Isolation of Mycobacterium tuberculosis:a 2-Year Study. R. S. MARTIN,* R. K. SUMARAH, AND E. M. ROBART .--------438
Anaerobic Specimen Transport Device. TRAcy D. WILKINS* AND FRANKLIN JIME-NEZ-ULATE.441
Longitudinal, Serological Study of Cytomegalovirus Infections in Nurses and inPersonnel Without Patient Contact. ANNE S. YEAGER.448
Methods to Improve Detection of Pneumococci in Respiratory Secretions. JOHNA. DILWORTH, PAULA STEWART, JACK M. GWALTNEY, JR., J. OWEN HENDLEY, AND45MERLE A.SANDE*45
Unusual Colonies of Ureaplasma urealyticum (T Mycoplasmas) in Primary AgarCultures of Certain Urine Sediments. M. C. SHEPARD AND C. D. LUNCE- 456FORD.
Selective Medium for Growth of Proteus. M. E. XILINAS,* J. T. PAPAVASSILIOU,AND N. H.LEGAKIS.459
Modified Fluorescent Antibody Technique to Detect Immunoglobulin M Antibodiesto Toxoplasma gondii in Congenital Infection. P. W. ROBERTSON* AND VERAKERTESZ.461
Color Reaction Streak Test for Catalase-Positive Microorganisms. JACOB S.HANKER* AND ALBERT N.RABIN.463
CONTENTS xiii
Volume 2 Contents for December Number6Sensitivity of Infectious Bovine Rhinotracheitis Virus to Ether. ROBERT A. CRAN-
DELL,* ANN J. MELLOH, AND PAMELA SORLIE.465
Direct Inoculation Procedure for the Rapid Classification of Bacteria from BloodCulture. STEPHEN C. EDBERG,* MARTA NOVAK, HELEN SLATER, AND JACQUESM. SINGER.469
New Microtechnique for the Leptospiral Microscopy Agglutination Test. PHILIPL. CARTER* AND TERENCE J. RYAN.474
Isolation of Yersinia enterocolitica from Swine. S. TOMA* AND VELMA R.DEIDRICK.478
Detection of Aspergillus fumigatus Serum Precipitins by Counterimmunoelec-trophoresis. THOMAS H. DEE.482
Nationwide Epidemic of Septicemia Caused by Contaminated Intravenous Prod-ucts: Mechanisms of Intrinsic Contamination. DONALD C. MACKEL, * DENNISG. MAKI, ROGER L. ANDERSON, FRANK S. RHAME, AND JOHN V. BENNETT .... ... 486
Improved Transport System for Neisseria gonorrhoeae in Clinical Specimens.DQOROTHY A. SYMINGTON.498
Influence of the Collection and Transport of Specimens on the Recovery ofBacteria from Peritonsillar Abscesses. HANS 0. HALLANDER,* ANDERS FLOD-STROM, AND KENNETH HOLMBERG.504
Counterimmunoelectrophoresis of Pneumococcal Antigens: Improved Sensitivityfor the Detection of Types VII and XIV. JOHN P. ANHALT AND PAULINE K.W. Yu.510
Experiments on Terminal Disinfection by Formaldehyde Vapor in the Case ofSmallpox. K GROSSGEBAUER,* G. SPICKER, J. PETERS, E. KUWERT, H. D.POHLE, AND H. KERNER.516
Epidemiologic Study of a Leukocyte-Transforming Agent in a General Population.CIRO V. SUMAYA.520
Simple Procedure for the Removal of Nonspecific Inhibitors of Rubella VirusHemagglutination. RICHARD ALLEN AND KENNETH HEDLUND*.524
Isolation of Streptococcal Nuclease B by Batch Adsorption. THOMAS F. SLECHTAAND ERNEST D. GRAY*.528
Application of the Single Radial Diffusion Test for Assay of Antibody to In-fluenza Type A Viruses. S. R. MOSTOW,* G. C. SCHILD, W. R. DOWDLE, ANDR. J. WOOD 531
Evaluation of a Microscopy Method for Rapid Detection and Identification ofMycoplasma pneumoniae. W. BREDT,* W. LAM, AND J. BERGER.541
Rapid Screening of Veillonella by Ultraviolet Fluorescence. ANTHONY W. CHOW,*VALERIE PATTEN, AND LUCIEN B. GUZE.546
Retention of Mercurial Preservatives in Desiccated Biological Products. JOHNR. PEMBERTON.549
Polypectate Digestion by Yersinia. V. LYLE VON RIESEN.552Convenient, Simplified Preparation of Less Commonly Used Media. V. LYLE
VON RIESEN.554Isolation of Vibrio alginolyticus from Wound Infections. SALLY Jo RUBIN*
AND RICHARD C. TILTON 556Clinical Isolation of Yersinia enterocolitica: Cold Temperature Enrichment. J. R.
GREENWOOD, S. M. FLANIGAN, M. J. PICKETT,* AND W. J. MARTIN.559
CONTENTSX1V