More on Microscopes(moron microscopes?)
Field of View,Resolving Power
and Depth of Field
Field of View• Portion of specimen viewable at the same
time• As magnification increases -> Field of view
decreases.• Lowest power objective has widest field of
view. That’s why it is used to “scan” the slide.
Resolving Power• Ability to distinguish two points as
separate and distinct instead of smooshing them together.
• Impressionist Art• As magnifying power increases ->
Resolving power increases…to a point
Sooooooooooooo……..
Where was resolution seen in the last lab?
Depth of field• Depth or “zone”, from background to foreground
that is in focus at once
Shallow DOF Deep DOF
Depth of field• In microscopes, as magnifying power
increases -> Depth of Field decreases (gets shallower)
Sooooooooooooo……..
Where was depth of field seen in the last lab?