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TreeAzin Systemic
Insecticide: a New Option forPest Management
J. MeatingBioForest Technologies Inc.December 1, 2010
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Acknowledgements
Canadian Forest Service;
B. Helson, J. McFarlane, D. Thompson
Ontario Ministry Natural Resources
National Research Council IRAP;
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TreeAzin Systemic Insecticide 5% technical systemic
formulation
Rapid uptake and
translocation
Effective in deciduousand conifer species
IP developed and
owned by the CFS
Under worldwide
license to BioForest
Technologies
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TreeAzin Systemic Insecticide
Neem-based reduced risk bio-
insecticide;
AI=Azadirachtin not Neem OilHigh toxicity to wide range of
damaging insect pests;
Very low mammalian and birdtoxicity;
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Azadirachtin: Modes of Action Immature stages: IGR
inhibits development
Mature stages:Repellant
Reduced fecundity
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TreeAzin: Regulatory Status in the U.S.
U.S.USEPA registration October 2008 (Reg #
82996-1)
Professional Use
Master Label Homeowner use
State registrations ~25
Canada
Emergency Registration for EAB (ON,QU)
Full Registration 2011?
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Environmental FateWhat happens when treated leaves
fall to the ground or into water
bodies?
AZA concentrations almostundetectable
No impact on soil organisms
No impact on aquatic organisms
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TreeAzin: OMRI Listed (Dec. 2008)
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TreeAzin Efficacy Trials 2002-2010
Host Species Red pine
Jack Pine
White pine
White spruce
Balsam fir
Cedar
Lodgepole pine
Red and Bur oak
Trembling aspen
Ash Maple
White birch
Hemlock
Pest Species
Emerald ash borer
Gypsy moth
Pine false webworm
Introduced pine sawfly
Cedar leafminer
Forest tent caterpillar Eastern spruce
budworm
Jack pine budworm
Birch leafminer
Bronze birch borer
Hemlock wooleyadelgid
*Mountain pine beetle
*Asian longhornedbeetle
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TreeAzin Label Pests Emerald ash borer Gypsy moth
Forest tent caterpillar
Spruce budworm
Jack pine budworm
Leafminers Sawflies
Hemlock Woolly Adelgid
Future additions:
Bronze Birch Borer
Asian Longhorned Beetle Japanese Beetle
Pine Shoot Beetle
Winter moth
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Emerald Ash Borer
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EAB Larval Development and AdultEmergence
Number of Developed Adult EAB Beetles in Imidacloprid andAzadirachtin Treated Potted Ash Trees
0
2
4
6
8
10
12
14
0 0.0017 0.0035 0.007 0.015 0.03 0.06
Imidacloprid
Azadirachtin
NumberofBeetles
Concentration in g of AI/cm dbh
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EAB Larval Development and AdultEmergence
Number of Incomplete EAB Beetle Galleries in Imidacloprid andAzadirachtin Treated Potted Ash Trees
0
2
4
6
8
10
12
0 0.0017 0.0035 0.007 0.015 0.03 0.06
Imidacloprid
Azadirachtin
Concentration in g of AI/cm dbh
NumberofG
alleries
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EAB Fecundity & Fertility
36.5
5.4
12.3
0.0905
10
15
20
25
30
35
40
Eggs laid Eggs hatched
Control TreeAzin
99% reduction in EAB population for Year X+1
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EAB: Evidence for Two-Years
Protection with TreeAzin
Oakville Study, March 2010
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Results: Year 2Treated August 2008
Feb. 2010: Trees assessed for
woodpecker feeding sites and 2009EAB galleries (n=209)
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Woodpecker Feeding Site Density
23
2
5
1
19
0
5
10
15
20
25
0 1-5 >5
Woodpecker feeding sites
No.
trees
Treated Untreated
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EAB Galleries: Yr 2EAB/100cmsq
0.08
0.47
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
TreeAzin Control
Galleries/100cmsq
Avg Lt. (cm)
2.4
10.2
0
2
4
6
8
10
12
TreeAzin Control
AvggalleryLength(cm)
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Efficacy: Oakville 2008-2010Heavily infested tree
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Conclusions: TreeAzinNo direct mortality of feeding
adults;
Some gallery formation;
Highly active at inhibiting larvaldevelopment and preventing adult
emergence at very low dosages;
Reduced fertility and fecundity ofadults feeding on leaves.
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CFS Conclusions: TreeAzin
Early summer injections at 12.5ml/in.
(5ml/cm) dbh at early stages of an
infestation should provide very effective
control of EAB.
McKenzie et al. (2010) Azadirachtin: an
effective systemic insecticide for control
of Agrilus planipennis (Coleoptera:Buprestidae). J. Econ. Entomol. 103 (3)
708 -717.
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TreeAzin: Municipal Programs EABDSS
implementation
Detection survey
training and
analysis
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BioForest Technologies Inc.
Thank you.
www.bioforest.ca
http://www.bioforest.ca/http://www.bioforest.ca/