•  Cripto  Loss  of  Func0on  (  Cripto  LOF)                                Guardiola  et  al.,  PNAS  2012  

•  Cripto  Gain  of  Func0on  (Cripto  GOF)  

Modelli  Murini  condizionali  in  cellule  staminali  muscolari  

(satelli0)  

• OR1-­‐Mobilizzazione  delle  cellule  staminali  muscolari  (satelli0)  in  modelli  murini  di  danno  muscolare  acuto  

•  OR2  -­‐  Iden0ficazione  di  nuovi  regolatori  delle  cellule  staminali                          (Automazione  HTS)  

IGB  “A.B.T.”  Gabriella  MinchioK  

Approcci  gene0ci  e  farmacologici  alla  rigenerazione  muscolare   • Proteina  Cripto  

ricombinante  • Delivery  sistemico  -­‐Biopolimeri  

Approcci  farmacologici  

 RNA  Seq/cellule  satelli0  

Cripto  LOF  

96-channel head disposable pipetting tips

The system executes Protocols for targeted differentiation of stem cells Screening of compounds libraries

Advantage/benefits High standard reliability, performance and flexibility Low user intervention Contamination risk reduction

Method standardization Up to 4000 single compuonds simultaneously screened

Complete sterility

Robotic platform: The Cell maker

liquid-handling workstation

laminar air-flow cabinet

medium reservoirs

library MTPs

MTP reader

cell culture MTPs

The CellMaker

+

Cell-based Phenotypic Screening: Identificazione di nuovi regolatori delle

cellule staminali

Cell Proliferation

Cell Colony Phenotype

Targeted Differentiation

•  LOPAC •  HDAC

Inhibitors

Small molecules

Casalino et al., Journal Molecular Cell Biol, 2011 Casalino et al., Molecular Biotechnology, 2011 Franci et al., under review

Laura Casalino

Eduardo J. Patriarca

Dario De Cesare Sandro De falco

Automation Core

Carolina Prezioso

Ombretta Guardiola Gennaro Andolfi

Collaborazioni

Giorgia Imparato Paolo Netti IIT Napoli