Honolulu Cloning Technique
By: Ashley Boggs
What is the Honolulu Cloning Technique?
A technique that uses adult cells and nuclei for cloningUsed to clone three generations of miceFirst clone was known as Cumulina
Honolulu Cloning
First used in July of 1998Experiment was at the University of HawaiiDeveloped by Ryuzo Yanagimachi and Teruhiko Wakayama
Steps of the Honolulu Technique
1. The cell’s nucleus is removed2. A donor nucleus is injected into an
egg3. The new cell is chemically cultured
to jumpstart cell growth
Steps of the Honolulu Technique Continued
4. This created embryos5. Which are placed
into the surrogate mothers to be carried
The method is repeated over and over to create 2nd and 3rd generations of identical mice
Pros of the Technique
Used to create clones, exact copies; genetic replicasHad very successful nuclei used, cumulus cellsMore successful cloning rate than the Rosalin techniqueCan help scientists do further research on egg reprogramming in a nucleus
Cons of Cloning
It was a process of trial and error, so before there were good clones there were many mistakes and mutations madeReduces genetic diversityExpensiveOne virus could knock out an entire species of clones because of a lack of genetic diversity
Uses of Honolulu Cloning
To further cell researchTo produce transgenic animalsTo produce drugs from transgenic animalsUse clones to “evaluate molecular mechanisms”Critique current techniques and create better ones
The End.