CorDynamics conducts thrombosis experiments – using small and large animals

Mouse ferric chloride model of arterial thrombosis

1) instrumentation to monitor carotid artery flow

- monitor artery flow prior to and after FeCl3

- FeCl3 migrates to lumen, results in endothelial injury

2) administer putative anti-thrombotic prior to FeCl3

-monitor for development of thrombus

-use real-time monitor of blood flow, time to occlusion

Pharmacological validation

- heparin (anticoagulant)

MOUSE THROMBOSIS MODEL

Effects of heparin on carotid artery blood flow

MOUSE THROMBOSIS MODEL

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

-5 5 15 25 35 45 55 65

Car

oti

d B

loo

d F

low

(m

L/m

in)

Time (minutes)

Effects of Heparin on Ferric Chloride (3.5 %)-Induced Carotid Arterial Thrombosis in the Mouse

[vertical line is FeCl3 exposure]

Vehicle (0.9% NaCl, n=5)

Heparin (200U/kg, n=5)

Effects of heparin on time to occlusion

MOUSE THROMBOSIS MODEL

0

10

20

30

40

50

60

70

Vehicle (0.9% NaCl, n=5) Heparin (200U/kg) (n=5)

Tim

e (m

inut

es)

Effects of Heparin on the Ferric Chloride (3.5%)-Induced Carotid Arterial Thrombosis in the Mouse

Occlusion Time

No occlusion

For more information, on this model and our other drug discovery and drug safety

capabilities, please visit us at www.cordynamics.com