CURRICULUM VITAE
Shagufta Moin, M.D.
DESIGNATION AND PLACE OF WORK Associate Professor
Department of Biochemistry
Jawaharlal Nehru Medical College
Aligarh Muslim University
Aligarh 202002, (U.P.), INDIA. Tel: +91 98 972 17346
Email: [email protected]
HOME/RESIDENCE
44, Sagar Housing Complex
Near CDF P.O., Anoop Shahar Road
Aligarh 202002, (U.P.), INDIA.
Landline No. 0571-2522418
ACADEMIC/EDUCATIONAL QUALIFICATIONS
Qualification Year of Institution award
M.D.(Biochemistry) 1998 J.N. Medical college , A.M.U., Aligarh
Passed in First Attempt
D.G.O. 1994 J.N. Medical college , A.M.U., Aligarh
Passed in First Attempt
M.B.B.S. 1991 J.N. Medical college , A.M.U., Aligarh
All Professionals passed
in First Attempt
Internship 1992 J.N. Medical college , A.M.U., Aligarh
B.Sc. (Hons.) Botany 1985 Women‗s College, A.M.U., Aligarh.
MD Thesis Title: Binding of bilirubin to erythrocytes from healthy smokers and tobacco
chewers.
MCI Registration Number : 10369
Date and Place of Registration : 02.11.1992, New Delhi
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TEACHING & RESEARCH EXPERIENCE
Designation Institution Duration Particulars of work
Associate Department of Biochemistry August 22, Teaching of M.B.B.S., B.D.S,
Professor J.N. Medical college, 2008 till M.D.S., & Dialysis technique A.M.U., Aligarh date students, guidance of M.D., M.S. and Ph.D. students.
Reader Department of Biochemistry August 22, Teaching of M.B.B.S., B.D.S,
J.N. Medical college , 2005 to M.D.S., & Dialysis technique A.M.U., Aligarh August 21, students, guidance of
2008 M.D.,M.S. and Ph.D. students.
Lecturer Department of Biochemistry December Teaching of M.B.B.S., B.D.S,
J.N. Medical college , 30, 1998 to M.D.S., D.M.L.T., Diploma in A.M.U., Aligarh August 21, Pharmacy & Dialysis technique
2005 students and research.
Demonstrator Department of Biochemistry February 25, Teaching of M.B.B.S., B.D.S,
J.N. Medical college , 1997to Dec M.D.S., D.M.L.T., Diploma in A.M.U., Aligarh 29, 1998 Pharmacy & Dialysis technique
students and research.
B.Sc. (Hons.) Home Science 1st
year (Hygiene and Physiology) 12years
B.Sc. (Hons.) Home Science 2nd
year (Nutritional Biochemistry) 07 years
TOTAL TEACHING EXPERIENCE : over 18 years 10 months
(02years and 01 months as junior resident)
RESEARCH PROJECTS OPERATED/SUBMITTED
PUBLICATION SUMMARY
Published in International Journal : 19
Published in National Journal : 08
International Conferences : 09
National Conferences : 14
Chapter in Books : 01
Communicated to International Journal : 05
Research work presented in International &
National Conferences /Symposium : 29
Training, Refresher, Orientation Courses : 12
Participation in Conference
Workshop, Symposium, CME : 72
Chairing of scientific Session : 02
Invited speaker/ Resource person : 11
2
MAJOR SCIENTIFIC FIELDS OF INTEREST/RESEARCH INTEREST / RESEARCH SPECIALIZATION
Albumin-Bilirubin interaction.
Free radical in health and disease.
Antioxidant and Antiglycation effect of Phytochemicals.
RESEARCH SUPERVISION
Number of student(s) awarded M.D./M.S./M.D.S. degree : 16 Number of student(s) enrolled for M.D./M.S./M.D.S. degree : 04 Number of student(s) awarded Ph.D.degree : 01 Number of student(s) enrolled for Ph.D.degree : 03 Number of student(s) enrolled for M.Phil. : -
Research Guidance Details
(A) M.D./M.S./M.D.S.
S.No. Name of the student Year of Title of Thesis
award
1. Dr. Prince Cheriyan 2003 Clinical, Biochemical and Tumor marker
Modayil, study in malignancy of nose and maxilla.
M.S.[E.N.T.]
(as Co-guide)
2. Dr. Gaurav Kumar 2008 Maternal and fetal indicators of oxidative
Gupta stress in premature/preterm labor.
M.D.[Biochemistry]
3. Dr. Gini Garima 2009 A study on the role of dietary agents in
M.D.[Biochemistry]
protein glycation.
4. Dr. Monal Vashishta 2009 A study of role of oxidative stress markers in
Sharma primary open angle glaucoma.
M.S.[Opthalmology]
(as Co-guide)
5. Dr. Sufia Naseem 2010 Studies on glycated HSA – its implications in
M.D.[Biochemistry]
diabetes mellitus.
6. Dr. Saeeda Wasim 2012 Quantitative evaluation of total antioxidant
M.S.[Obstetrics
capacity (TAC), Malondialdehyde (MDA)
and Thyroid stimulating hormone (TSH)
&Gynaecology]
levels in women with PIH
(as Co-guide)
3
7. Dr. Wajahat Ali Rizvi 2012 Estimation of serum electrolytes(Na+ & K+)
M.S.[Opthalmology]
and serum lipids in patients of senile cataract
and normal individuals of the same age- A
(as Co-guide)
comparative study
8. Dr. Puneet 2012 Comparative study of plasma ascorbic acid
Parasurampuria
and serum nitric oxide levels in senile
cataract patients and normal individuals.
M.S.[Opthalmology]
(as Co-guide)
9. Dr. Namita Gupta 2013 Effect of type 2 diabetes mellitus and
[M.D.S., Perio]dontics
smoking on salivary MMP-8 in chronic
periodontitis patients-A cross sectional study.
(as Co-guide)
10. Dr.Deepti Wadhwa 2013 Determination of levels of Nitric Oxide in
[M.D.S., Periodontics] smoker and non smoker patients with chronic
(as Co-guide) periodontitis.
11. Dr.Ricky Mittal 2014 Biochemical studies on oxidative stress
[M.D.,Biochemistry]
markers in type II diabetics-Role of vitamin
D.
12. Dr.Sushma Sharma 2014 Serum alkaline phosphatase as a bio-marker
[M.D.S.,
of skeletal and dental maturation.
Orthodontics]
(as Co-guide)
13. Dr.Pritma Singh 2014 Impact of smoking and type II diabetes
[M.D.S., mellitus on salivary TNF-alpha level in
,Periodontics] chronic periodontitis patients.
(as Co-guide)
14. Dr.Mohammad Zavir 2014 Evaluation of host factors and identification
Khan of bacterial speceis in patients suffering from
[M.D.S., Oral &
odontogenic infections.
Maxillofascial
surgery]
(as Co- guide)
4
15. Dr.Mazhar Alam 2014 Comparison of serum pro-calcitonin level
[M.D., T.B.
between pulmonary tuberculosis and
community acquired pneumonia.
&Respiratory
diseases]
(as Co-guide)
16. Dr.Humaira Siddiqui 2014 Evaluation of oxidative stress markers in
[M.S. Obstetrics
maternal and cord blood: vaginal delivery
versus elective caesarian section.
&Gynaecology]
(as Co-guide)
17. Dr.Asma Zoya submitted Comparative evaluation of radicular peroxide
[M.D.S.,
penetration from various bleaching agents
during intracoronal bleaching.
Periodontology]
(as Co-guide)
18. Dr.Pankaj Bansal submitted To compare and correlate salivary TNF-alpha
[M.D.S.,
levels in patients with desquamative
gingivitis and chronic gingivitis.
Periodontology]
(as Co-guide)
19. Dr.Kundan Kumar submitted To study the protective effect of olive oil on
[M.S. Anatomy]
the development of atherosclerosis in aorta of
rabbit.
(as Co-guide)
20. Dr.Neha Bansal submitted Evaluation of correlation between vitamin C
[M.D.S., Periodontics]
and total antioxidant capacity in chronic
periodontitis.
(as Co-guide)
(B) Ph.D.
S.No. Name of candidate Year of Title of Thesis
award
1. Miss. Shazia Aman 2014 Antioxidant and antiglycating effect of
phytochemicals.
2. Miss. Naureen Fatima Continuing Functional and clinical relevance of TH-17
cells in diabetes mellitus.
3. Miss. Somaiya Mateen Continuing Role of pro-inflammatory cytokines in
establishment of rheumatoid arthritis.
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LIST OF RESEARCH PUBLICATIONS 1. Role of serum procalcitonin level in differentiating between pulmonary tuberculosis
and community-acquired pneumonia. Mohammad Shameem, Mazhar Alam, Shagufta
Moin, Rakesh Bhargava, Zuber Ahmad, Jamal Akhtar. International Journal of
Clinical Medicine. Vol. 5, pp. 902-909, 2014. [Impact Factor]
2. Segments of spinal cord harboring motor neuron somata of radial nerve of rabbit.
Farhan Kirmani, Nema Usman, Shagufta Moin. Journal of Evolution of Medical
and Dental Sciences. Vol. 3(26) pp. 7112-15, 2014. [Impact Factor]
3. Evaluation of oxidative stress markers in maternal and cord blood: Vaginal delivery
versus Elective caesarean section. Humaira Siddiqui, Nasreen Noor, Shagufta Moin,
Shazia Parveen. International Journal of Current Medical Research. Vol. 3, No. 2,
pp. 024-027, 2014. [Impact Factor]
4. Oxidative stress and antioxidant enzymes in pre-eclampsia. Shazia Parveen, Nasreen
Noor, Shagufta Moin, Zakia Arshad, Naheed Banu. Journal of South Asian
Federation of Obstetrics and Gynecology. Vol. 6(1), pp. 5-7, Jan-Apr, 2014.
5. Serum Na+ and K+ as risk factors in age related cataract: An Indian perspective.
Adeeb Alam Khan, Syed Wajahat A. Rizvi, Abadan K. Amitawa, Shagufta Moin,
Ziya Siddiqui, Faraz Yusuf. Sudanese Journal of Ophthalmology. Vol. 6(1). Jan-
Jun, 2014. [Impact Factor]
6. A study to evaluate the antioxidant and hepatoprotective activity of aqueous extract of
roots of Valeriana wallichii in CCl4 induced hepatotoxicity in rats. S.N. Syed, W.
Rizvi, A. Kumar, A.A. Khan, S. Moin, A. Ahsan. International Journal of Basic
and Clinical Pharmacology. 3(2): 354-358, 2014.[Impact Factor ]
7. Antioxidant and Hepatoprotective Activity of Ethanolic Extract of Valeriana
wallichii in CCl4 treated rats. S.N. Syed, W. Rizvi, A. Kumar, A.A. Khan, S. Moin,
A. Ahsan. British Journal of Pharmaceutical Research. 4(8): 1004-1013, 2014.
8. Study to Evaluate the Antioxidant and Hepatoprotective Activities of Roots Extracts
of Doronicum hookeri in CCl4 Treated Rats. S.N. Syed, W. Rizvi, A. Kumar, A.A.
Khan, S. Moin, and P.A. Khan. European Journal of Medicinal Plants 4(6): 675-
685, 2014
9. In vitro antioxidant and in vivo hepatoprotective activity of leave extract of Raphanus
sativus in rats using CCl4 model. S.N. Syed, W. Rizvi, A. Kumar, A.A. Khan, S.
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Moin, A. Ahsan. African Journal of Traditional Complementary and Alternative
Medicine. 11(3):102-106, 2014. 10. Determination of levels of nitric oxide in smoker & non-smoker patients with chronic
periodotitis : Deepti Wadhwa, Afshan Bey, Mukesh Hasija, Shagufta Moin, Arun
Kumar, Shazia Aman, Vivak Kumar Sharma. J.Periodontal & Implant Science
2013; 43;215-220. [Impact Factor .No impact has been given till date] pISSN 2093-
2278, eISSN 2093-2286
11. Study of serum nitric oxide levels in senile cataract patients and in normal individuals.
Puneet Parasurampuria , Adeeb Alam Khan, Mohammad Ashraf, Shazia Aman and
Shagufta Moin. International Journal of Scientific Research.2013; 2(6):9-11.
[Impact Factor .33] ISSN No 2277-8179
12. Antioxidant activity of thymoquinone and its protective effect against oxidative
haemolysis. Shazia Aman, Shagufta Moin. International Journal of Scientific
Research.2013; 2(4):28-30.[Impact Factor .33] ISSN No 2277-8179
13. Nigella sativa as an antiglycating agent for human serum albumin. Tariq Mahmood,
Shagufta Moin, Abul Faiz Faizy, Sufia Naseem and Shazia Aman. International
Journal of Scientific Research.2013; 2(4):25-27.[Impact Factor .33] ISNN No 2277-
8179
14. Antioxidant activity of thymol: Protective role in AAPH-induced hemolysis in
diabetic erythrocytes. Shazia Aman, Shagufta Moin, M Owais and MU Siddiqui.
International Journal of Pharmaceutical Science Invention.2013; volume 2 issue
3:55-60. [Impact Factor ] ISSN (Online): 2319-6718, ISSN (Print): 2319-670X
15. In vivo study of Histamine H4 receptor in immuno modulation. Tripathi T, Shahid M,
Khan HM, Siddiqui M, Khan RA, Shujat Ullah F, Moin S, Malik A. Bratis Lek
Listy.2012; 113(11):641-7. [Impact Factor .34] pISSN 0006-9248; eISSN 1336-0345
16. Erythrocyte markers of oxidative stress in higher age-group pre-eclamptic and normal
pregnant mothers, Syed K. Haque, M.U. Siddiqui, N. Islam and Shagufta Moin,
Hypertension in Pregnancy 29(1): 69-81 (2010). [Impact Factor 1.52] ISSN: 1064-
1955 print/ 1525-6065 online, cited by 5.
17. Histamine, Histamine Receptors and their Role in Immuno modulation: An Updated
Systematic Review. M. Shahid, T. Tripathi, F. Sobia, S. Moin, M.U. Siddiqui and
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R.A. Khan. The Open Immunology Journal, 2, 9-41 (2009). [Impact Factor .25]
ISSN 1874-2262, cited by 28.
18. Normal delivery induced stress alters glutathione peroxidase and TNF- in elderly
primigravidas mononuclear cells. N. Noor, N. Islam, S. Moin, A.A. Mahdi, S. Jaiswal
and F. Bano. Indian Journal of Clinical Biochemistry, 2008/23 (3), 227-232 (2008).
[Impact Factor .19 ] pISSN 0970-1915, eISSN 0974-0422
19. Tuftsin-mediated immunoprophylaxis against isolate of Aspergillus fumigatus
showing less in vivo susceptibility to amphotericin B. M.A. Khan, N. Ahmad, S.
Moin, A. Mannan, H. Wajahul, S.T. Pasha, A. Khan and M. Owais. FEMS
Immunology and Medical Microbiology, Vol. 44, pp. 269-276 (2005).
Elsevier.[Impact Factor 1.779]
20. Liposome – Bilirubin Interaction: A novel strategy to remove bilirubin from systemic
circulation. M.A. Khan, S. Moin, S. Tayyab, A.F. Faizy, M.U. Siddiqui and M.
Owais. Journal of Liposome Research, Vol. 14, Nos. 3 & 4, pp. 111-122
(2004).[Impact Factor 2.02]
21. Study on Bilirubin Erythrocyte Interaction in diabetic patients. A. Rahman, S. Moin,
M.U. Siddiqui. Indian Journal of Bioresearch, Vol. 50, No. 1, pp. 150-155 (2003).
22. Increased bilirubin binding to erythrocytes of tobacco chewers than non-chewers. S.
Moin, M. Qutub Ali, M.U. Siddiqui and S. Tayyab. Current Science, Vol. 77, No. 2,
25 July (1999). [Impact Factor ]
23. Erythrocytes from healthy smokers bind more bilirubin than the erythrocytes from
healthy non-smokers. S. Moin, M.Q. Ali, M.U. Siddiqui and S. Tayyab. Molecular
and Cellular Biochemistry, 183: 211-214 (1998) Netherlands. [Impact Factor 1.76] ARTICLES PUBLISHED
1. Inherited unconjugated hyperbilirubinemias -A clinical overview.
Shagufta Moin. Indian Journal of Bioresearch. Jan-June 2004; 56(1): 7-9.
2. Programmed Cell Death—Apoptosis, Shagufta Moin. Indian Journal of
Bioresearch.2003; 50(2):74-75
3. Gene Therapy, Shagufta Moin. Indian Journal of Bioresearch. Jan-June 2003;
53(1):44-46.
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4. DNA Vaccines: Designer vaccine for the 21st
century .Shagufta Moin. Indian
Journal of Bioresearch. July-Dec. 2003; 53(2) :158-159. RESEARCH PAPERS ACCEPTED FOR PUBLICATION / COMMUNICATED 1. Serum ALP activity: As a Biomarker of Skeletal And Dental Maturation
Journal of Indian Orthodontic Society (JPJ 1642250714
European Journal of Orthodontics (EJO – 2014- OA 029 )
2. ― Salivary TNF - alpha : A correlation with gingival desquamations‖
Journal of Clinical Periodontology, CPE-11-14-5398
3. Colligation of Total Antioxidant Capacity (TAC) with Periodontal Disease
and
Treatment
Journal of Clinical Periodontology CPE-11-14-5405
4. Unravelling the intricacies of tumor- immune system interrelationships
hindwani
PUBLICATION OF CONFERENCE PRESENTATIONS IN JOURNALS
1. A study on the levels of Antioxidant defense – Enzymes in Pregnancy Induced
Hypertension. S.K. Haque, M.U. Siddiqui, N. Islam and S. Moin. Indian Journal of
Clinical Biochemistry Proceedings. 2007, Vol. 22 (Supl.), ISSN 0970-1915.
2. A study on the levels of Antioxidant defense – Enzymes in Preterm Labour. G.K.
Gupta, N. Islam and S. Moin. Indian Journal of Clinical Biochemistry
Proceedings. 2007, Vol. 22 (Suppl.), ISSN 0970-1915.
3. Effect of EGCG a green tea polyphenol, on the levels of TNF-alpha and glutathione
peroxidase activity in pre-eclampsia patients. R. Alam, S. Moin, N. Noor, G.
Garima, F. Bano and N. Islam. Indian Journal of Clinical Biochemistry
Proceedings. 2007, Vol. 22 (Suppl.), ISSN 0970-1915.
4. Antiglycating Effect of Dietary Agents, S. Moin, N. Noor, R. Alam and S. Khan.
Indian Journal of Clinical Biochemistry Proceedings. 2007, Vol. 22 (Suppl.),
ISSN 0970-1915.
RESEARCH WORK PRESENTED AT CONFERENCES AND SYMPOSIA
1. Naureen Fatima, Shagufta Moin, Nasreen Noor, Humaira Siddiqui. Evaluation of
oxidative stress markers in maternal and cord blood: vaginal delivery verses elective
caesarian section. National symposium on recent advances in free radical biology and
biochemistry under the auspices of UGC-SAP-DRS-III. Department of Biochemistry,
Faculty of Life Sciences. AMU, Aligarh, 06 March 2014.
2. Naureen Fatima, Shireen Hasan, Shagufta Moin, Naheed Banu. Modulatory Role of
Multivitamin Mineral Supplement on Chronic Unpredictable Stress (CUS) and
Dietary Restriction (DR) induced Biochemical Changes in Mice. UGC-SAP
sponsored National Conference on ―Chronic Inflammatory Disorders‖ organized by
D/o Biochemistry, F/o Science & D/o Medical Biochemistry, F/o Medicine (HIMSR)
on Feb 13-15, 2014.
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3. Ricky Mittal, Shagufta Moin, Neha Mittal. Biochemical Studies on Oxidative Stress
Markers in Type 2 Diabetics- Role of Vitamin D. 3rd
Annual Meeting of the Indian
Academy of Biomedical Sciences & Symposium on Modern Trends in Human
Diseases. Department of Biochemistry, Faculty of Medicine, JNMC, AMU, Aligarh.
14-15 Dec, 2013.
4. Shazia Aman, Shagufta Moin, Mohammad Owais, M. U. Siddiqui. Assessment of
Antioxidant Potential of Eugenol: Its Implications in Diabetes. 3rd
Annual Meeting of
the Indian Academy of Biomedical Sciences & Symposium on Modern Trends in
Human Diseases. Department of Biochemistry, Faculty of Medicine, JNMC, AMU,
Aligarh. 14-15 Dec, 2013.
5. Naureen Fatima, Naheed Banu, Shagufta Moin. Role of Chronic Unpredictable
Stress and Dietary Restriction on Oxidant-Antioxidant Status in Mice. 3rd
Annual
Meeting of the Indian Academy of Biomedical Sciences & Symposium on Modern
Trends in Human Diseases. Department of Biochemistry, Faculty of Medicine,
JNMC, AMU, Aligarh. 14-15 Dec, 2013.
6. Shagufta Moin, Shahnawaz Hasan and Shazia Aman. A study on Oxidative Stress
Markers in Iron Deficient Pregnant Anaemic Women. 21st
Annual Conference of
Medical Biochemists of India, Christian Medical College & Hospital, Ludhiana
(Punjab) 15-17 Nov-2013. 7. Shagufta Moin, Shazia Aman, M. Owais, M.U. Siddiqui, Protective role of Eugenol
against AAPPH induced haemolysis in diabetic erythrocytes, International
Conference on recent advances in diabetes and endocrinology, Rajiv Gandhi Centre
for Diabetes and Endocrinology, Aligarh Muslim University, Aligarh, 15-17 March
2013. 8. Shazia Parveen, Zakia Arshad, Nasreen Noor, Shagufta Moin, Naheed Banu,
Oxidative stress and antioxidant enzymes in pre-eclampsia, International Conference
on recent advances in diabetes and endocrinology, Rajiv Gandhi Centre for Diabetes
and Endocrinology, Aligarh Muslim University, Aligarh, 15-17 March 2013.
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9. Nasreen Noor, Shahnawaz Hasan, Shazia Aman, Shazia Parveen and Shagufta Moin,
Evaluation of oxidative stress and antioxidant status in iron deficient pregnant anemic
women. International Conference on recent advances in diabetes and endocrinology.
Rajiv Gandhi Centre for Diabetes and Endocrinology, Aligarh Muslim University,
Aligarh, 15-17 March 2013.
10. Shagufta Moin, Shazia Aman, M.Owais, M.U. Siddiqui, Protective effect of
thymoquinone against AAPH induced haemolosis in diabetic erhythrocytes,
International Conference on Chemistry: Frontiers and Challenges, Department of
Chemistry, Aligarh Muslim University, Aligarh, India [Under UGC-SAP(DRS-I)],
March 2-3, 2013.
11. Shagufta Moin, Shazia Aman, M. Owais, M.U. Siddiqui, Assessment of antioxidant
potential of thymol: Its implication in diabetes, International Conference on Advances
in Free Radicals, Redox Signaling and Translational Antioxidant Research and XII
Annual Meeting of the Society for Free Radical Research, India, Organized by CSIR,
Indian Institute of Toxicology Research, Lucknow, Jan. 30 to Feb. 1, 2013.
12. Shazia Aman, M. Owais, M.U. Siddiqui and Shagufta Moin, Evaluation of
Antioxidant activity of Eugenol, International Conference on Advances in Free
Radicals, Redox Signaling and Translational Antioxidant Research and XII Annual
Meeting of the Society for Free Radical Research, India, Organized by CSIR, Indian
Institute of Toxicology Research, Lucknow, Jan. 30 to Feb. 1, 2013.
13. Shagufta Moin, Shazia Aman, M. Owais, M.U. Siddiqui, Evaluation of antioxidant
activity of thymoquinone: Possible role in diabetes mellitus, XX National Conference
of Association of Medical Biochemist of India, Bhubaneshwar, Organized by AMBI
Odisha State Chapter sponsored by UGC, 7-10 December 2012.
14. Shagufta Moin, Shazia Aman, Tariq Mehmood, Evaluation of Antioxidant activity of
Salvadora persica extract, Ist Annual Conference of Association of Physiologists and
Pharmacologists of India UP-UK Chapter, Organized by Department of Physiology
and Pharmacology, J.N. Medical College, AMU, Aligarh,6-7 October 2012.
11
15. Shazia Aman, Tariq Mehmood, Shagufta Moin, Assessment of Antioxidant activity
of Withania somnifera extract, Ist Annual Conference of Association of Physiologists
and Pharmacologists of India UP-UK Chapter, Organized by Department of
Physiology and Pharmacology, J.N. Medical College, AMU, Aligarh,6-7 October
2012. 16. S. Naseem, M.U. Siddiqui, S. Moin, S. Hasan, S. Aman, N. Noor., Glycation of
Human Serum Albumin with Fructose and its implications in Diabetes mellitus,
International Conference on Advances in Free Radicals Research, Natural Products,
Antioxidants and Radioprotectors in Health, Nizam‗s Institute of Medical Science,
Hyderabad and University of Hyderabad, Jan. 11-13, 2010.
17. S. Aman, S. Naseem, S. Moin, M.U. Siddiqui, N. Noor., Biochemical Studies on
Glycated Human Serum Albumin, International Conference on Advances in Free
Radicals Research, Natural Products, Antioxidants and Radioprotectors in Health,
Nizam‗s Institute of Medical Science, Hyderabad and University of Hyderabad, Jan.
11-13, 2010. 18. G. Garima, S. Moin, R. Alam, S. Khan, N. Noor and M.U. Siddiqui, Protective effect
of aged garlic extract in protein glycation, International Conference on Advances in
Free Radical Research and 8th
Annual Meeting of the Society for Free Radical
Research, India, Department of Biochemistry, CSM Medical University, Lucknow
and Era‗s Lucknow Medical College, Lucknow, India, March 19-21, 2009.
19. T. Tripathi, M. Shahid, S. Moin, M.U. Siddiqui and R.A. Khan, Histamine receptors
in immunodulation, 4th
Annual Conference of U.P. Chapter of Indian Association of
Medical Microbiologist UP MICROCON-2008, CSM Medical Univ. Lucknow,
February 16, 2008. 20. S.K. Haque, M.U. Siddiqui, N. Islam and S. Moin, A study on the levels of
Antioxidant Defense Enzymes in pregnancy induced hypertension, 34th
Annual
Conference of Clinical Biochemists of India (ACBI CON-2007), India Habitat Centre,
New Delhi, December 18-20, 2007.
12
21. G.K. Gupta, N. Islam and S. Moin, A study on the levels of Antioxidant Defense
Enzymes in Preterm Labor, 34th
Annual Conference of Clinical Biochemists of India
(ACBI CON-2007), India Habitat Centre, New Delhi, December 18-20, 2007.
22. R. Alam, S. Moin, N. Noor, G. Garima, F. Bano and N. Islam, Effect of EGCG a
Green tea Polyphenol, on the level of TNF- and glutathione peroxidase activity in
Pre-ecclampsie, 34th
Annual Conference of Clinical Biochemists of India (ACBI
CON-2007), India Habitat Centre, New Delhi, December 18-20, 2007.
23. S. Moin, N. Noor, R. Alam, and S. Khan, Antiglycating Effect of Dietary Agents,
34th
Annual Conference of Clinical Biochemists of India (ACBI CON-2007), India
Habitat Centre, New Delhi, December 18-20, 2007. 24. S. Moin, R. Alam and S.Khan, Evaluation of Anti-OxLDL antibodies in pregnancy
induced hypertension, 19th
U.P. Congress of Obstetrics and Gynaecology, Deptt. of
Obs. & Gyn. AMU, November 24 & 25, 2007.
25. S. Moin, M.S. Akhtar and M.U. Siddiqui, Binding of Bilirubin to erythrocytes from
cancer patients, ―International Conference on Anti-oxidants and Free Radicals in
health-nutrition and Radio-protectors‖ & IV Annual Conference of the Society for
Free Radical Research in India (SFRR), St. Johns Medical College, Bangalore,
January 10-12, 2005.
26. S Kumar, M. Aslam and S. Moin, Serum Copper & Serum Amylase Levels in
Nasomaxillary Cancers, Indian Society of Oncology, North Zone Conference, Aligarh
Muslim University, Aligarh, December 20-21, 2003.
27. S. Moin, N. Yusuf, M.U. Siddiqui and N. Islam., The Role of Reactive Oxygen
Species (ROS) in the formation of an alternate autoantigen in the Myocarditis and
DCM, International Conference on Role of Free Radicals and Antioxidants in Health
and Disease 2nd
Annual Conference of SFRR-INDIA, King George Medical College,
Lucknow, February 10-12, 2003.
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28. A.Rahman, S. Moin and M.U. Siddiqui, Binding of bilirubin to erythrocytes obtained
from diabetic patients, International Conference on Role of Free Radicals and
Antioxidants in Health and Disease 2nd
Annual Conference of SFRR-INDIA, King
George Medical College, Lucknow, February 10-12, 2003.
29. S. Moin, M.Q. Ali, S. Tayyab and M.U. Siddiqui, Binding of bilirubin to erythrocytes
from healthy smokers and non-smokers, 65th
Annual Meeting of the Society of
Biological Chemists, India, Indian Institute of Science, Bangalore, November 20-23,
1997.
CHAIRING OF SCIENTIFIC SESSIONS
Chaired a session in the National Symposium on Emerging Trends in Biological
Sciences: Special Emphasis on Neurosciences, 24-25 September, 2012. Organized by
Interdisciplinary Brain Research Centre, Faculty of Medicine, J.N.M.C., A.M.U.,
Aligarh
Chaired a session in the 3rd
Annual Meeting of the Indian Academy of Biomedical
Sciences & Symposium on Modern Trends in Human Diseases, December 14
&15,2013. Organized by Department of Biochemistry Faculty of Medicine, J.N.M.C.
,A.M.U.,Aligarh BOOK CHAPTERS: Chapter contributed in the book Biomedical Aspects of Histamine: New Perspectives published by Springer.
Possible Effect of Histamine in Physiology of Female Reproductive Function – An
Updated Review. N. Noor, T. Tripathi, S. Moin, A.F. Faizy. Biomedical Aspects of
Histamine: New Perspectives (Springer) 2010.
14
INVITED SPEAKER / RESOURCE PERSON
1. Faculty in U.G. Orientation Programme held at J.N. Medical College, AMU,
Aligarh, 1st
and 2nd
Sep, 2014.
2. Invited as a faculty to participate in Validation /Moderation Workshop of MCQ
Question Bank on 26 & 27 August 2014 at NBE Office, Dwarka, New Delhi. Ref No
NBE/VAL-WORKSHOP/MCQ/AUG/2014/102.
3. Delivered a lecture in Interdisciplinary Subject Refresher Course in Environmental
Studies at UGC Academic Staff College on Teaching Skills, 21.08.2014. .
4. Delivered a lecture in Interdisciplinary Subject Refresher Course in Environmental
Studies at UGC Academic Staff College on Ultraviolet rays (UV rays) - Some
interesting facts , 14.08.2014.
5. Delivered a lecturer in P.G. Orientation Programme on Teaching Skills at J.N.
Medical College, AMU, Aligarh, 01 July 2014.
6. Delivered a lecture in Interdisciplinary Subject Refresher Course in Environmental
Studies at UGC Academic Staff College on Sunshine & Strong Bones, 17.05.2012 .
7. Delivered a lecture in Interdisciplinary Subject Refresher Course in Environmental
Studies at UGC Academic Staff College on Sunshine & Strong Bones, 15.05.2012.
8. Delivered a lecture in Interdisciplinary Subject Refresher Course in Environmental
Studies at UGC Academic Staff College on Biochemical changes in humans due to
UV rays exposure, 28.07.2010.
9. Delivered a lecture in FOGSI Postgraduate CME on HIV in Pregnancy in the
Department of Obstetrics and Gynaecology, J.N. Medical College, AMU, Aligarh, 13
December 2009.
10. Delivered a lecturer in P.G. Orientation Programme on Rational Use of Laboratory
Investigations (Biochemical) at J.N. Medical College, AMU, Aligarh, 20 August
2009.
11. Delivered a Lecture on Water and Water Born Diseases in the Department of
Geography, AMU, Aligarh to the participants of Refresher course, 28 March 2003.
15
SEMINAR / SYMPOSIUM / CME / WORKSHOP / CONFERENCES ATTENDED 1. National CME on ―Pharmacotherapy Update in Common Medical Disorders- A Rational
Approach‖ organized by Department of Pharmacology, J.N. Medical College, AMU,
Aligarh. Nov. 1-2, 2014. 2. CME on Managing Medical Records ―Embedding Substantial Potential Benefit into
Practice‖ organized by Central Record Section of J.N. Medical College and Hospital,
AMU, Aligarh, 12th
May, 2014. 3. CME on ― Rational use of Blood Components‖, Department of Pathology, J. N. Medical
College and Hospital. 24 th
March 2014. 4. CME on Central Sterilization Services Principles and Practice, organized by J. N. Medical
College and Hospital, A.M.U., Aligarh. 22 nd
March 2014. 5. National Workshop cum CME on Alternatives to Animal Experimentation in Medical
Science Education ( partially sponsored by MCI), Department of Pharmacology, J.N.
Medical College, A.M.U., Aligarh. 24-25 February 2014.
6. 3rd
Annual Meeting of the Indian Academy of Biomedical Sciences & Symposium
on Modern Trends in Human Diseases, December 14 &15,2013. Organized by
Department of Biochemistry Faculty of Medicine, J.N.M.C. ,A.M.U., Aligarh.
7. 21st
Annual Conference of Association of Medical Biochemists of India,held at
Christian Medical College,Ludhiana 15th
-17th
November, 2013. 8. Workshop on ―Soft Skills in Medical Education‖organized by Christian
Medical College Ludhiana on 14-11-2013. 9. Hands on Workshop on ―How to write and Publish a case report‖ organized by
Department of Medicine, Jawaharlal Nehru Medical College, A.M.U., Aligarh in
scollaboration with ‗ BMJ Case Reports‗ on 14-08-2013. 10. International Conference on Recent Advances in Diabetes and Endocrinology, 15-17
March, 2013 organized by Rajiv Gandhi Centre for Diabetes and Endocrinology,
Aligarh Muslim University, Aligarh in collaboration with Indraprastha Apollo Centre for
Obesity, Diabetes and Endocrinology. 11. International Conference on Chemistry: Frontiers and Challenges, March 2-3, 2013,
Department of Chemistry, Aligarh Muslim University, Aligarh, India [Under UGC-SAP
(DRS-I)]. 12. Foundation Day Symposium on Current Trends in Biotechnology, Jan. 16, 2013
organized by Interdisciplinary Biotechnology Unit, AMU, Aligarh.
16
13. International Conference on Advances in Free Radicals, Redox Signaling and Translatinal
Antioxidant Research in XII Annual Meeting of the Society for Free Radical Research-
India, Jan. 30 to Feb. 1, 2013 organized by: CSIR-Indian Institute of Toxicology
Research, Lucknow. 14. XX National Conference of Association of Medical Biochemists of India, organized by
AMBI Odisha State Chapter sponsored by UGC.
15. 1st
Annual Conference of Association of Physiologists and Pharmacologists of India,
UP-UK Chapter, jointly organized by Department of Physiology and Pharmacology, J.N.
Medical College, AMU, Aligarh. 16. National Symposium on Emerging Trends in Biological Sciences: Special Emphasis on
Neurosciences, 24-25 September, 2012 organized by Interdisciplinary Brain Research
Centre, Faculty of Medicine, A.M.U., Aligarh. 17. Popular Lectures on Neuroscience and Nanotechnology (A DBT funded Programme)
organized by Interdisciplinary Brain Research Centre, (IBRC) Faculty of Medicine,
AMU, Aligarh, 29 December 2012. 18. Symposium cum live workshop on Chronic Back Pain organized by Department of
Anaesthesiology and Critical Care, J.N. Medical College, AMU, Aligarh in Association
with Indian Society for Study of Pain, 22 Dec. 2012.
19. National Symposium on Medical Ethics: Challenges and Future Prospects, 9th
Sept.
2012, Medical Education Unit, Faculty of Medicine, AMU, Aligarh. 20. Golden Jubilee CME on metamorphosis: Channelling the change for betterment of
Medical Education in India, 31st
Mach 2012, Medical Education Unit, Faculty of
Medicine, AMU, Aligarh. 21. Silver Jubilee Symposium on Emerging Trends in Biochemical and Toxicological
Sciences, Department of Biochemistry, Faculty of Life Sciences, A.M.U., Aligarh,
March 06, 2012.
22. 23rd
Annual Conference of UP Chapter of Obstetrics and Gynaecology of India, 19-20
November, 2011 organized by Agra Obstetrics & Gynaecology Society and Department
of Obs. & Gynae. S.N. Medical College, Agra. 23. Scientific Programme on Pharmacovigilance conducted by Knowledge Academy on 17
November 2011, J.N. Medical College, A.M.U., Aligarh.
24. Symposium on Emerging Trends in Dengue and other haemorrhagic fevers in India, 8th
October 2011 organized by Department of Microbiology, J.N. Medical College,
A.M.U., Aligarh. 17
25. Workshop on Student Assessment, 7-8 March 2011, Medical Education Unit, Faculty of
Medicine, AMU, Aligarh. 26. International Conference on Chemistry: Frontiers and Challenges, 5-6 March 2011,
Department of Chemistry, AMU, Aligarh. 27. National Symposium on Current Trends and Biochemical, Biomedical, and
Environmental Sciences, 22 February 2011, Department of Biochemistry, Faculty of
Life Sciences, AMU, Aligarh.
28. International Conference of Integrative Medicine and 1st
SAARC Conference of Unani
Medicine, 2-4 February 2011 organized by Faculty of Unani Medicine, AMU, Aligarh. 29. Malaria – An update organized by Department of Microbiology, J.N. Medical College,
A.M.U., Aligarh, 18 December 2010.
30. AMBICON-2010, 18th
National Conference of Association of Medical Biochemist of
India, Hyderabad, 12-14 November, 2010. 31. Workshop on Techniques in Haemoglobinopathies, 11 November 2010 at Tapadia
Diagnostic Centre, Hyderabad. 32. Faculty Development Programme on Medical Education Technology, 05.04.2010, J.N.
Medical College, AMU, Aligarh. 33. National Symposium on Sports Physiology Today and Tomorrow organized by
Department of Physiology, J.N. Medical College, AMU, Aligarh. 34. FOGSI Postgraduate CME, Department of Obstetrics and Gynaecology, JNMC, AMU,
Aligarh, December 13, 2009. 35. Scientific Programme on Basics of Clinical Research, Piramal Knowledge Academy,
Medical Education Unit, JNMC, AMU, Aligarh, December 8, 2009. 36. CME LIFE (Let Induction Fulfill Existence), AOGS, Department of Obstetrics and
Gynaecology, JNMC, AMU, Aligarh, October 3, 2009. 37. CME Gynae Endocrinology, under aegis of FOGSI and DST, Department of Obstetrics
and Gynaecology, AIIMS, New Delhi, April 18, 2009. 38. International Conference on Advances in Free Radical Research, Natural Products,
Antioxidants and Radio-protectors and 8th
Annual Meeting of the Society for Free
Radical Research India, Department of Biochemistry, C.S.M. Medical University &
Eras Lucknow Medical College, Lucknow, March 19-21, 2009. 39. International SFRR Satellite Symposium 2009 on Free Radicals in Health and Disease,
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University,
Aligarh, March 17-18, 2009.
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40. CME Programme on Role of Letrazole in Ovulation Induction, Department of Obstetrics
and Gynecology, J.N. Medical College, A.M.U., Aligarh, Feb. 21, 2009. 41. Orientation and Sensitization Workshop on Science Writing/Journalism, National
Council for Science and Technology Communication, DST, New Delhi, Department of
Mass Communication, AMU, Aligarh, December 6-10, 2008. 42. Continuing Medical Education and Workshop-2008 on Mechanical Ventilation and ABG
Analysis in Association with Indian Society of Critical Care Medicine, Department of
Anaesthesiology and Critical Care, JNMC, AMU, Aligarh, December 12, 2008. 43. National Symposium on Advances in Clinical Biochemistry Biomarkers, Molecular
Diagnosis and QUALITY Diagnosis and Quality Assurances & 1st
U.P. Chapter of
Association of Clinical Biochemists of India (UPACBICON), Department of
Biochemistry, J.N. Medical College, AMU, Aligarh, November 15-16, 2008. 44. Interdisciplinary Science Conference-2008 Mathematics in Biology, Centre for
Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi. 45. National Workshop on Prevention of Lead Poisoning, Department of Biochemistry,
C.S.M. Medical University, Lucknow, 29th
March 2008. 46. Molecular Medicine Update – 2008, Maulana Azad Medical College, Delhi, March, 28,
2008. 47. International Symposium on the Predictive, Preventive and Mechanistic Mutagenesis
and XXXIII EMSI Annual Meeting, EMSI, Department of Agriculture, AMU, Aligarh,
January 1-3, 2008.
48. 34th
Annual Conference of Clinical Biochemistry of India, ACBICON-2007, New Delhi,
December 18-20, 2007. 49. CME & Workshop on Stem Cell Therapy, Escort Heart Institute and Research Ltd., New
Delhi, December 17, 2007.
50. 19th
U.P. Congress of Obstetrics and Gynaecology, UPCON-2007, Obstetrics and
Gynaecology Society, J.N. Medical College, AMU, Aligarh, November 24 & 25, 2007. 51. CME on HIV/AIDS Prevention and Treatment Awareness, National AIDS Control
Organization and Indian Medical Association, April 22, 2007. 52. National Symposium on Relevance of Pharmaco-Vigilance for Indian Systems of
Medicine, Under the aegis of Society of Pharmaco-Vigilance, India, Department of
Pharmacology, J.N. Medical College, A.M.U., Aligarh, November 4, 2006.
19
53. ICOG CME on Ultrasound and Cardiotocography, Aligarh Obstetrics and Gynaecology
Society (AOGS), Department of Obstetrics and Gynaecology, J.N. Medical College,
AMU, Aligarh, March 2, 2006. 54. XIV Annual Conference of Indian Association of Pathologists and Microbiologists (U.P.
Chapter), Department of Pathology, J.N. Medical College, AMU, Aligarh, November
23, 2005. 55. Workshop on Colposcopy and Management of Cervical Pre-cancer, Department of
Obstetrics and Gynaecology, J.N. Medical College, AMU, Aligarh, March 20-21, 2005. 56. International Conference on ―Antioxidants and Free Radicals in Health-Nutrition and
Radioprotectors‖ and IV Annual Conference of SFRR-India, Department of
Biochemistry, St. John‗s National Academy of Health Sciences, January 10-12, 2005. 57. Cardio Diabetology Update-2004, Endocrinology Division, Department of Medicine, J.J.
Medical College, AMU, Aligarh, October 9-10, 2004. 58. ISO, North Zone Confernece-2003, Indian Society of Oncology, Department of
Radiotherapy, J.N. Medical College, AMU, Aligarh, December 20-21, 2003. 59. CME Programme, Indian Society of Anaesthesiologist/WFSA, J.N. Medical College,
AMU, Aligarh, November 29-30, 2003. 60. North Zone CME in Endocrinology, Endocrine Society of India, MCI, J.N. Medical
College, AMU, Aligarh, September 15-16, 2003. 61. Live IUI Workshop, Aligarh Obstetrics and Gynaecology Society, Department of
Obstetrics and Gynaecology, J.N. Medical College, AMU, Aligarh, May 11, 2003. 62. Issues in Medicine - An Update, Department of Medicine, J.N. Medical College, AMU,
Aligarh, March 29-30, 2003. 63. International Conference on Role of Free Radicals and Antioxidants in Health and
Disease and 2nd
Annual Conference of SFRR-INDIA, Department of Biochemistry,
C.S.M. Medical University, Lucknow, February 10-21, 2003. 64. Indo-US Workshop on Problem Based Learning in Medical Education, J.N. Medical
College, AMU, Aligarh, November 21, 2001. 65. Reproductive and Child Health Workshop, Aligarh Obstetrics and Gynaecological
Society in Collaboration with Federation of Obstetrics and Gynaecological Societies of
India and MOHFW, Govt. of India and WHO, J.N. Medical College, AMU, Aligarh,
September 22-23, 2001. 66. Bioinformatics Workshop on Information Retrieval through Internet for the study of
Protein Structure and Other Applications, Department of Biochemistry, Ministry of
20
Science and Technology, Govt. of India, New Delhi, Distributed Information Sub-
Centre, Biotechnology Building, AMU, Aligarh, January 9-11, 2001.
67. 10th
Annual Conference, U.P. Chapter of Obstetrics and Gynaecology, Aligarh Obstetrics
and Gynaecological Sociewty, J.N. Medical College, AMU, Aligarh, November 22-23,
2007. 68. Introduction to the use of Personal Computers and Window Based Softwares in
Bioinformatics with Special Reference to NICNET Connectivity and CD-ROM
Databases, Department of Biochemistry, Ministry of Science and Technology, Govt. of
India, New Delhi, Distributed information Sub-Centre, Biochemistry Building, AMU,
Aligarh, March 10-12, 1997.
69. 65th
Annual Meeting of the Society of the Biological Chemists (India), Department of
Biochemistry, Indian Institute of Science, Bangalore, November 20-23, 1996. 70. Workshop on Advanced Gynecological Endoscopy, Department of Obstetrics and
Gynecology, J.N. Medical College, AMU, Aligarh, July 26-27, 1994. 71. Mid-Term Endocrine Conference and CME, Endocrinology and Metabolic Section,
Department of Medicine, J.N. Medical College, AMU, Aligarh, July 26-27, 1994. 72. Comprehensive Postgraduate Teaching Course (CME), under the auspices of AOGD,
Department of Obstetrics and Gynaecology, UCMS and GTB Hospital, Delhi, April 16-
17, 1994.
TRAINING / REFRESHER / ORIENTATION / PROFESSIONAL COURSES ATTENDED
1. MCI Advance Course in Medical Education held from 29th
Sep 2014 – 2nd
Oct 2014
at MCI Nodal Centre for Faculty Development, NHL Municipal Medical College,
Ahemdabad.
2. Medical Council of India, Basic Course Workshop in Medical Education
Technologies, held by the Regional Centre Maulana Azad Medical College, New
Delhi during 29 April- 02 May 2014.
3. Short Term Course on Academic Paper Writing Skills held at UGC Academic Staff
College,AMU, Aligarh from 26 th
March – 02 nd
April 2014.
4. Training course on Application of Research Techniques in Reproductive Biomedicine
held at NIHFW, New Delhi from November 16-27, 2009.
21
5. Subject Refresher Course in Computer Applications (Interdisciplinary) held at UGC
Academic Staff College, AMU, Aligarh from December 21, 2008 to January 21,
2009.
6. Professional Course in Clinical Biochemistry organized by Escorts Heart Institute and
Research Centre Ltd., New Delhi from December 15-16, 2007.
7. Annual Training Course in Endocrinological Techniques and their Applications held
at National Institute of Nutrition, Hyderabad from August 21 to September 29, 2006.
8. 49th
National Teacher Training Course on Educational Science Technology for
Teachers of Health Professionals held at National Teacher Training Centre,
Department of Medical Education, Maulana Azad Medical College, New Delhi from
January 27, 2003 to February 05, 2003.
9. Training Course on Molecular Biology, Hybridoma and Cell Culture Techniques
conducted at National Biotechnology Centre, Indian Veterinary Research Institute,
Izatnagar from September 08-29, 2003 under the auspices of National Agricultural
Technology Project (NATP) on Human Resource Development in Veterinary
Biotechnology.
10. District Level Training of Trainers on HIV/AIDS conducted by JNMC, AMU,
Aligarh under the auspices of U.P. State AIDS Control Society, Nawal Kishore Road,
Lucknow from April 08-10, 2002.
11. Refresher Course in Biochemistry held at UGC Academic Staff College, AMU,
Aligarh from October 15 to November 06, 2001.
12. Orientation Programme in Biochemistry held at UGC Academic Staff College, AMU,
Aligarh from November 07 to December 07, 2001.
APPOINTED AS M.D., M.B.B.S., B.D.S., M.Sc.(Clinical Biochemistry), M.Sc.(Medical Biochemistry), B.Sc.(Home Science) EXAMINER
1. M.D., M.B.B.S., B.D.S. J.N. Medical College, A.M.U. Aligarh, U.P.
2. B.Sc. Hons.(Home Science), Women,s College, A.M.U. Aligarh, U.P.
3. M.B.B.S., S.N. Medical College, Agra, U.P.
4. M.B.B.S., Rohilkhand Medical College, Bareilly, U.P.
5. M.B.B.S., Sri Ram Murti Smarak Institute of Medical Sciences, Bareilly.U.P.
6. M.B.B.S., National Institute of Medical Sciences, Jaipur, Rajasthan.
7. M.B.B.S.& M.D., Himalayan Institute of Medical Sciences,Dehradun, Uttarakhand,
8. M.B.B.S., Subharti Medical College, Meerut, U.P.
22
9. M.B.B.S., Lala Lajpat Rai Memorial Medical College, Meerut,U.P.
10. B.D.S.&M.Sc.(MedicalBiochemistry),Teerthankar Mahavir University, Moradabad
U.P.
11. M.Sc. (Clinical Biochemistry), University of Kashmir, Srinagar.
12. M.B.B.S., All India Institute of Medical Sciences,Rishikesh,Uttarakhand.
13. M.B.B.S. and M.Sc.(Medical Biochemistry) Integral University , Lucknow , U.P.
APPOINTED AS PAPER SETTER
M.B.B.S., B.D.S.and M.Sc. (Medical Biochemistry), Teerthankar Mahavir University,
Moradabad, U.P.
Ph.D.entrance,Ph.D.CourseWork(PrePh.D).,&M.Sc.(MedicalBiochemistry),IntegralU
niversity,Lucknow, U.P.
M. D. Biochemistry, Rohilkhand University, Bareilly.
ADMINISTRATIVE RESPONSIBILITIES
Member of Committee to monitor overall cleanliness and ambience in the department.
Member of undergraduate and postgraduate syllabus revision committee.
Treasurer National CME on ―Pharmacotherapy Update in Common Medical
Disorders- A Rational Approach‖ organized by Department of Pharmacology, J.N.
Medical College, AMU, Aligarh. Nov. 1-2, 2014.
Member of reception and registration committee for National Symposium on
―Hospital Acquired Infections & Antimicrobial Resistance‖ held on 9th
Dec, 2014.
Warden, S.N. Hall, September 16, 2000 to June 2007.
Incharge Clinical Lab. of the Department of Biochemistry, ( 2000-2003).
Incharge for M.D. Seminar Lectures since October 2005.till date.
Scrutinizer for compulsory retotalling /scrutiny of marks for session 2013-14.
23
MEMBERSHIP/PARTICIPATION IN BODIES/COMMITTEES ON EDUCATION
Member, Executive Committee, FOGSI ( Aligarh Branch ).2005-2008 & February
2012 till date.
Member of the Faculty of Medicine, A.M.U. Aligarh for a period of two years w.e.f.
Appointed as Member of Board of Studies, Department of General Human
Physiology & Biochemistry, Teerthankar Mahavir University, Moradabad for a period
of two years w.e.f. 2009-2011.
Member of the Faculty of Medicine, w.e.f. from 29-8-2013 for a period of two years.
Member of Medical Education Unit, J.N. Medical College, A.M.U., Feb 2014.
CO-CURRICULAR ACTIVITIES
Active participation as Judge for various cultural and sport events during S.N. Hall
week celebration
Deputed to Jeddah as Lady Medical Officer to Indian Medical Mission Hajj in
1998, 1999 and 2002.
Participated in TV Shows on ETV Urdu e.g. ―Bazm-e-Niswan‖, Hajj Ek Ibadat,
Sehri Times, Tandrusti Hazar Niyamat, Quiz Line and Adaab Doctor.
Worked in a project entitled ―Epidemiological studies of Radiation Occupational
Workers and their Families at Atomic Power Project/Station Sites Nuclear
Power Corporation, Govt. of India (1996).
OUTREACH ACTIVITIES
Participated as a specialist in several health camps in and around Aligarh.
Participated in Pulse Polio Programme-2000
REVIEWER OF PAPERS Reviewed the papers for the following journals-
Indian Journal of Biochemistry and Biophysics
International Journal of Medicine and Medical Sciences
Biomed Research International 24
EDITORIAL MEMBERSHIP
Registered in the editorial board as Advisory board for the Journal BP J00000 232- International Journal of Medical and Clinical Research. LIFE MEMBERSHIP OF NATIONAL AND INTERNATIONAL SCIENTIFIC
BODIES /SOCIETIES
Life Member of the Society of Biological Chemists, India. Life Membership No.
1261 (2000).
Life Member of Association of Clinical Biochemists of India 2007. Life
Membership No. 2934.
Life Member of U.P. Chapter of Obstetricians and Gynaecologists from November
2007.
Life Member of Indian Medical Association. Membership No. UP/8467/2/174/
143865/2009-10/L (2009).
Life member of the Society of Biological Scientist of India from 20.02.2010,
Membership No. LM/SBSI/134.
Life member of the Association of Medical Biochemists of India. Membership No.
UP-05.
Member of the International Association of Lions Club ( Aligarh branch).
Life member of Indian Academy of Biomedical Sciences (IABS). Membership
number 96/2012
Life member of Indian Society for Lead Awareness & Research. Membership
number 29/2012
25
PARTICIPATION IN HEALTH SHOWS ON TELEVISION
From 2005 to 2010 participated as an Expert every year in the ―SEHRI TIME”,
Sehri Time is a Live Phone in show which telecast daily on ETV Urdu in the holy
month of RAMADHAN. If viewers have any query about any medical problem,
expert present in the studio give the answers of their problems. I participated as a
Gynaecologist and Medical Practitioner in two episodes every year. The programme
aired from 4:00 am to 5:00 am.
ETV Urdu airs another special show for Hajj Pilgrims on the occasion HAJJ. This is a
live phone in show titled ―HAJJ EK IBADAT‖ in which different Islamic scholars
along with medical experts, who have performed Hajj, give the answers of the
pilgrims. Since I was the part of Hajj Delegation in 1998, 1999 and 2002, I
participated as an Medical Practitioner and share my experiences on different aspects
of health and also give the answers of the questions of the pilgrims related to health
and care to be taken during Hajj. I was participating every year in two episodes from
2005-2006.
Participated as an Obstetrician and Gynaecologist in several episodes of
―TANDRUSTI HAZAR NIYAMAT‖ – a health based show on ETV Urdu. The
topics on which I participated was Breast Feeding, Antenatal and Postnatal Care,
Ovulation, Infertility, Women‗s Health, Tobacco Control, Nutrition in Pregnancy,
Contraceptives etc.
Actively involved in Live phone in health show on ―ETV Urdu‖, titled ―AADAB
DOCTOR‖. Adab Doctor is a bi-weekly show, in which different health related issues
are discussed and viewers in more than 52 countries ask their query about any medical
problem, to get the answers from the experts present in the studio. The programme
airs every Thursday and Friday from 5:00 to 6:00 pm. More than 125 episodes have
been telecast till date.
Actively involved for another Live Quiz Based show on ETV Urdu titled “QUIZ
LINE”. The programme is designed by keeping in mind the students upto Senior
Secondary Level. There are more than 20 topics on which questions are asked, the
subjects which I am handling are Science and Technology, The programme airs Every
Sunday from 10:30 pm to 11:30 pm.
26
PERSONAL DETAILS
Father‗s Name : Mr. Moind Uddin
Mother s Name : Mrs. Nafees Moin
Nationality : Indian (Passport No. )
Date of Birth : November 01, 1965
Marital Status : Married
Languages : English, Urdu, Hindi
REFEREES
1. Prof. M.U. Siddiqui Department of Biochemistry , Faculty of Medicine Aligarh Muslim University,Aligarh-202 002 (INDIA) E-mail: [email protected] Phone No.: 09837201776
2. Prof. Saad Tayyab Institute of Biological Sciences, Faculty of Science Building, University of Malaya 50603, Kuala Lumpur ,(MALAYSIA) E-mail: [email protected] Phone No.: 03-79677118 (office) Fax: 03-79674178
Teaching Material on
“Enzymes” for MBBS First
Professional Students, 2014 Batch
ENZYMES
Biologic polymers that catalyze chemical reactions that
make life possible.
BIOMEDICAL IMPORTANCE
Presence & maintenance of a complete & balanced set of
enzymes is essential for the breakdown of nutrients to
supply energy & chemical building blocks. These building
blocks assemble into proteins, DNA, membranes, cells &
tissues. Energy harnessed is used to power cell motility,
neural functions & muscle contraction.
The ability to assay the activity of specific enzymes in
blood, other tissue fluids or cell extracts aids in diagnosis
& prognosis of disease.
Enzymes can be employed in the clinical laboratory as
tools for determining critical metabolites e.g. glucose
oxidase is frequently utilized to measure plasma glucose
concentration.
Enzymes are also employed as tools for treatment of
injury & disease. Tissue plasminogen activator, or
streptokinase is used in the treatment of MI while trypsin
has been used in the treatment of cystic fibrosis.
Sensitivity of enzyme assays can be exploited to detect
proteins that lack catalytic activity in ELISA.
A complete balanced set of enzyme activities is of
importance for maintenance of homeostasis.
They are targets of choice for drugs that cure or
ameliorates human disease. Defective enzymes/ deficiency
in the quantity or catalytic activity of key enzyme can
result from genetic defects/ genetic mutations, nutritional
deficits or toxins/ infections by viral or bacterial
pathogens (e.g. vibrio cholerae)
INDUSTRIAL USES
Proteases & amylases augment the capacity of detergents
to remove dirt & stains.
Enzymes also play a role in producing or enhancing the
nutrient value of food products for both humans &
animals. The protease renin, for e.g. is utilized in the
production of cheese, while lactose is employed to remove
lactose from milk for the benefit of lactose-intolerant
persons deficient in this hydrolytic enzymes.
PROPERTIES OF ENZYMES
Enzymes catalyze conversion of one or more compounds
(substrates) into one or more different compounds
(products). They possess two important properties-
EFFICIENCY- enhance the rate of corresponding non-
catalyzed reaction by factors of atleast 106
SPECIFICITY- they are specific both for the type of
reaction catalyzed & for a single substrate or a small set of
closely related substrate. Enzymes are also stereo-specific
catalyst & catalyze reactions of only one stereo-isomer of
a given compound. For e.g. D- but not L- sugars, L- but
not D- amino acids.
Most of the enzymes are proteins, exceptions include
ribosomal RNAs, & self-cleaving or self-splicing RNA
molecules known collectively as ribozymes.
CO-FACTORS, CO-ENZYMES, PROSTHETIC
GROUPS
Many enzymes contain small non-protein molecules &
metal ions that participate directly in substrate binding &
catalysis. They are termed as prosthetic groups/ co-factors/
co-enzymes. Co-factors bind in transient dissociable
manner either to the enzymes or to the substrate. Most
common co-factors are metal ions. Enzymes that require a
metal ion as co-factor are termed metal activated enzymes.
Prosthetic groups are tightly & stably integrated into a
protein‘s structure by covalent & non covalent forces.
Metals are the main prosthetic groups. Enzymes that
contain tightly bound metal ions or metal ion as prosthetic
group are termed metallo enzymes.
Co-enzymes serve as recyclable shuttles or group transfer
agents that transport many substance from one point
within the substrate from others. B complex group of
vitamins supply important components of numerous co-
enzymes.
ENZYME CLASSIFICATION
1. Oxidoreductasescatalyze oxidations and
reductions, e.g. lactate dehydrogenase
2. Transferases catalyze transfer of groups such as
methyl or glycosyl groups from a donor molecule to an
acceptor molecule, e.g. hexokinase.
3. Hydrolases catalyze the hydrolytic cleavage of C-C,
C-O, C-N, P-O, and certain other bonds, including acid
anhydride bonds, e.g. acetylcholine esterase.
4. Lyases catalyze cleavage of C-C, C-O, C-N, and
other bonds by elimination, leaving double bonds, and
also add groups to double bonds, e.g. aldolase.
5. Isomerases catalyze geometric or structural changes
within a single molecule, e.g. phospho-triose
isomerase.
6. Ligases catalyze the joining together of two
molecules, coupled to the hydrolysis of a
pyrophosphorylgroup in ATP or a similar nucleoside
triphosphate, e.g. acetyl co A carboxylase.
The IUB name of ―hexokinase‖ is ―ATP:D-hexose-6-
phosphotransferaseE.C. 2.7.1.1.‖ This identifies
hexokinase as amember of class 2 (transferases), subclass
7 (transfer of aphosphoryl group), sub-subclass 1 (alcohol
is the phosphorylacceptor). Finally, the term ―hexose-6‖
indicatesthat the alcohol phosphorylated is that of carbon
six ofa hexose.
ACTIVE SITE
Recognition site for binding of substrates. Within active
site, substrates are brought into close proximity to one
another in optimal alignment with the co-factors,
prosthetic groups & amino acids side chains responsible
for catalyzing their chemical transformation into products.
Catalysis is further enhanced by the capacity of active site
to shield substrate from water, & generate an environment
whose polarity, hydrophobicity, acidity or alkalinity can
differ markedly from that of the surrounding cytoplasm.
MODELS FOR ENZYME CATALYSIS
Emil Fischer proposed that specificity with which
enzymes discriminate their substrates when forming an
enzyme substrate complex was analogous to the manner in
which a mechanical lock distinguishes the proper key. The
lock is formed by a cleft or pocket on the protein‘s surface
that forms part of a region called active site.
Lock and key model of enzyme- substrate binding
This model accounted for the exquisite specificity of
enzyme substrate interactions. The implied rigidity of
enzyme‘s active site failed to account for the dynamic
changes that accompany catalysis. This drawback was
addressed by Daniel Khosland‘s induced fit model which
states that when substrate approach & bind to an enzyme,
they induce a conformational change analogous to placing
a hand (substrate) into a glove (enzyme). The enzyme in
turn induces reciprocal changes in its substrates,
harnessing the energy of binding to facilitate the
transformation of substrate into products.
Induced-fit model of enzyme-substrate binding
MECHANISM TO FACILITATE CATALYSIS
Enzymes use various combinations of four general
mechanisms to achieve catalytic enhancement of the rates
of the chemical reaction
Catalysis by proximity
Acid-base catalysis
Catalysis by strain
Covalent catalysis
Enzymes of aspartic protease family (pepsin, lysosomal
cathepsin, HIV protease) illustrate acid-base catalysis.
Serine protease (chymotrypsin) illustrates covalent
catalysis.
Enzymes catalyzing lytic reactions bind their substrates in
a conformation that is unfavorable for the bond that will
undergo cleavage. This conformation mimics that of the
transition state intermediate, a transient specie that
represents the transition state or a half-way point, in the
transformation of substrate into products. In this state
neither free substrate nor product exits. Energy needed to
reach transition state is known as activation energy.
Stabilization of transition state is a general mechanism by
which enzymes accelerate the rates of chemical reactions.
Transition state analogs are more effective enzyme
inhibitors.
ENZYME KINETICS
It is the field of biochemistry concerned with the
quantitative measurement of the rates of enzyme catalyzed
reactions & the systematic study of factors that affect
these rates.
KINETICS OF ENZYME CATALYSIS
Enzymes accelerate the reaction rates by lowering
activation energy needed for the formation of transition
state. The environment of the active site lower activation
energy by stabilizing the transition state intermediate.
Enzymes bind to TSI more tightly than it does to either
substrates or products.
FACTORS AFFECTING THE RATES OF ENZYME
CATALYZED REACTIONS
Multiple factors affect the rate of enzyme catalyzed
reactions.
Temperature- raising the temperature increases the rates
of both un-catalyzed & enzyme catalyzed reactions by
increasing the kinetic energy & the collision frequency of
reacting molecules. Heat energy can also increase the
kinetic energy of the enzyme to a point that exceeds the
energy barrier for disrupting the non-covalent interactions
that maintains its 3-D structure. The polypeptide chain
then begins to unfold or denature with an accompanying
loss of catalytic activity. Enzymes from humans generally
exhibit stability at temperatures upto 45-55C.
Hydrogen ion concentration- most intracellular enzymes
exhibit optimal activity at pH values between 5 & 9.
Relationship of activity to hydrogen ion concentration is
due to enzyme denaturation at high or low pH & effects
on the charged state of the enzymes, substrates, or both.
Substrate concentration- for a typical enzyme, as
substrate concentration is increased Vi increases (due to
more formation of ES complex) until it reaches a
maximum value Vmax. Further increase in substrate
concentration do not further increase Vi (enzyme is
saturated with the substrate). Shape of the curve that
relates substrate concentration to activity is hyperbolic. At
any given instant only substrate molecules that are
combined with the enzyme as an ES complex can be
transformed into a product.
MICHAELIS-MENTEN EQUATION
Michaelis-Menten equation illustrates relationship
between initial reaction velocity Vi& substrate
concentration [S].
Vi = Vmax[S]/KM + [S]
Michaelis-Menten constant (KM), is the substrate
concentration at which the initial velocity is half of the
maximal velocity attainable at a particular concentration
of the enzyme.
LINEAR FORM OF MICHAELIS-MENTEN
EQUATION
The direct measurement of the KM&Vmax often requires
high concentration of substrates to achieve saturating
conditions. A linear form of theMichaelis-Menten
equation circumvents this difficulty & permits Vmax& KM
to be extrapolated from initial velocity data obtained at
less than saturating concentrations of the substrate.
A plot of 1/Vi as a function of 1/ [S], gives a straight line
whose Y intercept is 1/ Vmax& whose slope is KM/ Vmax.
Such a plot is called double reciprocal plot or Line
Weaver Burk plot. X-intercept is -1/ KM , KM is thus
readily calculated from negative x-intercept.
This plot can also be used to determine the kinetic
mechanisms of enzyme inhibitors.
SPECIFIC ACTIVITY, TURN OVER NUMBER,
CATALYTIC CONSTANT
To compare the relative activity of different enzymes or of
different preparations of the same enzyme, several
parameters may be used.
The activity of impure enzyme preparations is expressed
as specific activity (Vmax/ protein concentration).
For a homogeneous enzyme, one may calculate its turn
over number (Vmax/ moles of enzyme present).
If the number of active sites present is known, the
catalytic activity of a homogeneous enzyme is expressed
as its catalytic constantKcat (Vmax/ number of active sites).
Catalytic efficiency of the enzyme is the ratio of two
kinetic constants, Kcat / KM.
ENZYME INHIBITION
Inhibitors of catalytic activities of enzymes can be used
as-
pharmacologic agents
research tools for the study of mechanism of
enzyme action
The strength of interaction between an inhibitor & an
enzyme depends on forces important in protein structure
& ligand binding (hydrogen bonds, electrostatic
interactions, hydrophobic interactions & Van der Waal
forces)
Inhibitors can be classified on the basis of their
site of action on the enzyme
on whether they chemically modify the enzyme
on the kinetic parameter, they influence
Compounds that mimics the transition state of an enzyme-
catalyzed reaction (transition state analogs) or that takes
advantage of the catalytic machinery of an enzyme
(mechanism-based inhibitors) can be potent inhibitors.
Kinetically, we distinguish two classes of inhibitors based
upon whether raising substrate concentration does or does
not overcome the inhibition. Double reciprocal plot
facilitates the evaluation of inhibitors.
COMPETETIVE INHIBITION
Competitive inhibitors resemble substrate (substrate
analog). They bind to the substrate-binding portion of
active site thereby, blocking access by the substrate. Their
effect can be overcome by raising the concentration of
substrate.
Inhibition of the enzyme succinate dehydrogenase by
malonate illustrates competitive inhibition by substrate
analog.Competitive inhibitors act by decreasing the
number of free enzyme molecules available to bind
substrate, i.e. to form ES complex & thus eventually to
form product. A competitive inhibitor has no effect on
Vmax but raises the apparent KM for the substrate.
NON-COMPETETIVE INHIBITION
Anon-competitive inhibitor bear no or little structural
resemblance to the substrate & bind with enzyme at sites
distinct from the substrate binding site. Binding of
inhibitor does not affect the binding of substrate. So,
formation of both EI & EIS complexes is possible. While
E-I complex can still bind the substrate its efficiency at
transforming substrate to product is decreased or generates
product at a negligible rates. Their effects can never be
completely overcome by increase in substrate
concentration. Simple non-competitive inhibitors lower
Vmax but do not affect KM.
IRREVESIBLE INHIBITORS (POISON ENZYMES)
In reversible inhibition the inhibitors form a dissociable
dynamic complex with the enzyme. Fully active enzyme
can be recovered simply by removing the inhibitor from
the surrounding medium. Irreversible inhibitors act
irreversibly by chemically modifying the enzyme. These
modifications generally involve making or breaking
covalent bond with amino acyl residues essential for
substrate binding, catalysis or maintenance of enzyme‘s
functional conformation. These covalent changes are
relatively stable. So, enzyme remains inhibited even after
the removal of the remaining inhibitor from the
surrounding medium e.g. heavy metal atoms, acylating
reagents.
MECHANISM-BASED INHIBITION OR “SUCIDE
INHIBITORS”
Substrate analogs that contain a chemical group that can
be transformed by the catalytic machinery of the target
enzyme. After binding to the active site, catalysis by the
enzyme generates a highly reactive group that forms a
covalent bond & blocks the function of a catalytically
essential residue. They are promising leads for
development of enzyme specific drugs.
FEEDBACK REGULATION
Dietary cholesterol decreases hepatic synthesis of
cholesterol by controlling the expression of the gene that
encodes HMG-Co A reductase (enzyme repression).
FEEDBACK INHIBITION
Feedback inhibition is a process by which the end product
of a multi-step biosynthetic pathway binds to & inhibits an
enzyme catalyzing one of the early step in that pathway.
Feedback inhibitors bear little or no structural similarity to
the substrate of the enzyme they inhibit. They bind at an
allosteric site, spatially distinct from the catalytic site of
the target enzyme. A feedback inhibitors act as negative
allosteric inhibitor & inhibits the first committed step in a
particular biosynthetic sequence. Statins curtail synthesis
of cholesterol by inhibiting HMG-Co A reductase, which
catalyzes a rate limiting state of cholesterol biosynthesis.
In biosynthetic pathways where there are two or more end
products, each end product may only partially inhibit
catalytic activity. The effect of an excess of two or more
end products may be additive or greater than their
individual effect (known as co-operative feedback
inhibition).
PROENZYMES
Proteins that are synthesized & secreted as an inactive
precursor proteins are known as proproteins. Selective or
partial proteolysis converts proprotein to mature protein.
Proproteins forms of enzymes are termed proenzymes or
zymogens. The synthesis & secretion of protease as
proenzyme is to protect the tissue of origin from auto
digestion. E.g. of proproteins include pro-insulin,
pepsinogen, trypsinogen, chymotrypsinogen, several
factors of blood clotting & blood clot dissolution cascade,
& procollagen.
REGULATION OF ENZYME ACTIVITY
Catalytic capacity of the rate-limiting reaction in a
metabolic pathway is the product of concentration of the
enzyme molecules & their intrinsic catalytic efficiency.
Catalytic capacity can be influenced by changing the
quantity of enzyme present & by altering its catalytic
efficiency.
Regulation of enzyme quantity- body proteins are in a
state of dynamic equilibrium, in which they are
continuously synthesized & degraded (turnover). The
absolute quantity of an enzyme is the net balance between
its rate of synthesis & rate of degradation.
Control of enzyme synthesis can be achieved by induction
or repression. Induction of protein synthesis requires hours
to produce significant changes in overall enzyme level.
The ubiquitin-proteasome pathway is responsible both for
the regulated degradation of selected cellular proteins &
for the removal of defective, aberrant proteins. Changes in
protein level generally dominate when meeting long-term
adaptive requirement.
Changes in intrinsic catalytic efficiency can be achieved
by allosteric regulation (binding of dissociable ligands) or
by covalent modification. This can happen within seconds
& best suited for rapid & transient alteration in metabolite
flux.
ALLOSTERIC ENZYMES
Catalysis at the active site may be modulated by the
presence of effectors at an allosteric site. Allosteric sites
are physically distinct from the catalytic sites. They are
multimeric, bind to their substrate at multiple sites (co-
operative binding). Binding of allosteric regulator induces
a conformational change in the enzyme that encompasses
the active site. Shape of the curve that relates changes in
Vi to changes in substrate concentration is sigmoidal,
which is represented by Hill equation.
Two classes of allosterically regulated enzymes are K-
series&V-series. For K-series, the substrate saturation
kinetics is competitive, i.e. KM is raised without an effect
on Vmax. For V-series, the allosteric inhibitors lowers Vmax
without affecting the KM. Alteration in KM or Vmaxare due
to conformational changes at the catalytic site induced by
binding of the allosteric effector at its site. For K-series,
this conformational change may weaken the bonds
between substrate & substrate-binding residues. For V-
series this may be due to alteration in the orientation or
charge of catalytic residues.
ISOZYMES
They are physically distinct versions of a given enzymes,
each of which catalysis the same reaction. Isozymes arise
through gene duplication. They may exhibit differences in
properties such as sensitivity to particular regulatory
factor or substrate affinity that adapt them to specific
tissues or circumstances. Some isozymes may enhance
survival by providing a backup copy of an essential
enzyme. Lactate dehydrogenase is a tetrameric enzyme
consisting of two monomer types- H (for heart) M (for
muscle) that combined to yield five LDH isozymes.
HHHH (I1), HHHM (I2), HHMM (I3), HMMM (I4),
MMMM (I5). Tissue- specific expression of H & M genes
determines the relative proportions of each subunit in
different tissues. Isozyme I1 predominates in heart tissue
& I5 in the liver. Thus, tissue injury releases a
characteristic pattern of LDH isozymes that can be
separated by electrophoresis. Creatine kinase (CK) has
three isozymes CK-MM (skeletal muscle), CK-BB (brain),
& CK-MB (heart & skeletal muscle). CK-MB appears
within 4-6 hours of an MI, peaks at 24 hours & returns to
base line by 48-72 hours. Individual CK isozymes are
separable by electrophoresis, thus facilitating detection.
Reference- Harper‘s Illustrated Biochemistry (29th
edition)
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