Bioengineering Research Activities in Tsinghua University for Sustainable Development of
Green Energy and Chemicals,and Clean Environment
Xin-Hui XING (邢新会)Department of Chemical Engineering
Tsinghua University07-07-2011
2011 ASPIRE Forum at KAISTJuly 7, 2011
Global Opportunities and Challenges
• Resources• Energy• Environment• Health• Sustainability• Trust• …….
Global innovation
Close international relationship for education and research
Global Shortage in Water Resource in 2025
Biotechnology
• Medical Biotechnology
• Agricultural Biotechnology
• Industrial Biotechnology
• Environmental Biotechnology
BiocatalysisBiomass
•Biofuels
•Electricity
•Heat
•Chemicals
Biorefinery Will Replace Petrorefinery
•Plants•Animal wastes•Agricultural wastes•Organic garbage•Wastewater
-Sugar path-Lignin path-Organic path-Pretreatment-Unit operation
Industrial Biotechnology
Feedstocks For Industrial Biotech • Crops • Energy plants (starch, oils, etc) • Low-grade (LG) biomass (agricultural, forestry,
animal husbandry, organic wastes, etc.) • Other resources (microalgae, etc)
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Renewable, Low eco-impact, processing feasibility
Biorefinery for Renewable Biomass
B. Kamm,M. Kamm. Principles of biorefineries.Applied microbiology and biotechnology, 2004, 64: 137-145
Advanced Biotechnology, Biological and Bioprocess Engineering
Bio-related Areas in THU
•School of Life Sciences•Medical School
•Department of Chemical Engineering (DCE)Biological and Bioprocess Engineering
•Department of Engineering Physics•Department of Material Sciences and Engineering•Department of Environmental Science and Engineering•Department of Mechanics •Research Institute of Nuclear and New Energy
Biological and Bioprocess Engineering
Evolution and protein engineering
Nano-Biotechnology
Biocatalysis engineering
Cell culture engineering
Biomedical engineering
Bioseparation engineering
Environmental bioengineeringProfessors: 5
Associate professors: 7
Assistant professor: 2
Biocatalyst Technology
Bioprocess Engineering
Applied Biotechnology
•Founded in 1984
1. Evolution and Protein Engineering ◆ Evolution and protein engineering aim to create high performancemicrobial cell factory, understand the structure-function relationship ofproteins, and to engineer cells/proteins with improved and multifunctionalproperties for use in industry. Bioinformatics, genetic engineering, rationaldesign, directed evolution and microbial evolution engineering are playingkey roles in this direction.
Site-directed mutagenesis
Directed evolutionBioinformatics
Nano-structured and Multifunctional Biocatalysts
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底物产物荧光实时可视化定量追踪
生产监控 亲和纯化 生物催化
MBPGFP
酶
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底物底物产物产物荧光实时可视化定量追踪
生产监控 亲和纯化 生物催化
MBPGFP
酶
Realtime monitoring of enzyme production
Monitoring of Enzyme affinity separation
Monitoring of Enzyme reaction
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底物产物荧光实时可视化定量追踪
生产监控 亲和纯化 生物催化
MBPGFP
酶
固定载体
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底物底物产物产物荧光实时可视化定量追踪
生产监控 亲和纯化 生物催化
MBPGFP
酶
固定载体
固定载体
底物产物荧光实时可视化定量追踪
生产监控 亲和纯化 生物催化
MBPGFP
酶
固定载体
固定载体
底物底物产物产物荧光实时可视化定量追踪
生产监控 亲和纯化 生物催化
MBPGFP
酶
Realtime monitoring of enzyme production
Monitoring of Enzyme affinity separation
Monitoring of Enzyme reaction
Fusion Protein Technology for Design of GFP-labeling Proteins
•Linker engineering•Direction
ComputationDesign of fusion strategy
•Linker engineering•Direction
ComputationDesign of fusion strategy
2. Nano-Biotechnology
• Integration of nanotechnology and biotechnology• Creation of nano-crystals by aid of biofunctions• Control of biofunctions by novel nano materials
Phage display Nano-crystals Applications
3. Biocatalysis Engineering • Application of enzymes or whole cells in industrial
processes to produce commodity chemicals, fine chemicals,pharmaceuticals, novel biomaterials, and bioenergychemicals such as biodiesel, bio-hydrogen and bio-alcohol,by metabolic engineering, systems biology and syntheticbiology.
Fluorescent industrialMicrobes
Pilot fermentationActive center
• Technology for enzymatic biodiesel production with long enzyme activity stability
• Process integration for production biodiesel and 1,3-propandiol (PDO) • Technology transfer and industrialization
(20,000 tons biodiesel/year in China)by Prof. Dehua Liu
Industrialization of Enzymatic Biodiesel Production
CH2-OOC-R1
CH-OOC-R2
CH2-OOC-R3
R1-COOR'
R2-COOR'
R3-COOR'
+ +lipase
glycerolmethanol
BioHythane Production from Biomass
废弃生物质
能源化应用预处理
氢发酵 甲烷发酵
生态肥料
气体净化
BioHythane(H2+CH4)
原料池
新村镇
分布式能源供给
核心:1保留-2种群-3系统
闭合能源循环
4. Cell Culture Engineering • Plant cell culture and animal cell culture• Useful metabolite productions by culturing cells from
conventional Chinese medicinal herbs, and correspondingmetabolism research.
Hairy roots in bioreactor
Taxol productionCrocin production
5. Biopharmaceutical Engineering• Drug controlled/sustained delivery, antibody engineering and
enzyme engineering for pharmaceutical processing andproduction.
Drug controlled delivery
6. Bioseparation Engineering • Recovery and purification of bioactive products from plant and
microbial cells, large scale biocatalysis process and naturalresources as well, including organic acids, antibiotics, proteins,biodegradable materials and polypeptides.
PS/PMMA dimentional phase separation in supercritical CO2
Hollow-fiber membrane system
7. Environmental Bioengineering • Focusing on biotechnology applications for diverse wastewater
treatments by integration of aerobic and anaerobic microbes,oil or salt contaminated soil remediation, hazardous chemicalsdegradation and corresponding fundamental research.
Wastewater treatment technology for sludge reduction and low energy consumption Bioremediation technology for polluted soils
Thank You!