7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 1/12
STATISTICAL ANALYSIS/ RESULTS
Power Calculation:
Based on the cure rates of the phase II clinical trial on the efficacy of the apple cider
vinegar vs salicylic lactic acid in the treatment of common warts, which yielded 36%
and 74%, and setting alpha at 0.05, sample size of at least 51 lesions per group has
98% power.
A. Patients/ Subjects & Common Warts Sample – Descriptives
Sex Total
Apple Cider 5 23% 17 77% 22 49%
Salicylic Lactic Acid 11 48% 12 52% 23 51%
Total 16 36% 29 64% 45 100%
Male Female
Out of the total 45 subjects, 36% are male and 64% are female. On either group,
most of the patients are female (A = 77%, B = 52%). The mean age of the patients
who received apple-cider vinegar treatment is 22.68 + 15.8 years, while those of
the patients who received salicylic-lactic acid treatment is 24.61 + 15.5 years.
104 common warts in 45 patients were included in the study. 53 common warts in
22 patients were subjected to Apple Cider Vinegar treatment while 51 common
warts in 23 patients were subjected to Salicylic-Lactic Acid treatment. In the apple
cider group, the mean number of warts per patient was 2.41 + 1.9. In the salicylic-
lactic acid group, the mean number of warts per patient was 2.22 + 1.9.
Three patients (two from apple-cider group, one from salicylic-lactic acid group)
with a total of four common warts (3 from apple cider group, 1 from salicylic-lactic
acid group) were not able to comply with the 4-week maximum clinical trial.
Duration of Lesions (% per group)1 Mo - 3 Mos 4 - 6 Mos 7 - 11 Mos 1 Yr 2 Yrs > 2 Yrs TOTAL
Apple Cider Vinegar 6 (27%) 5 (23%) 4 (18%) 4 (18%) 3 (14%) 0 (0%) 22 (100%)
Salicylic-Lactic Acid 12 (52%) 4 (17%) 0 (0%) 4 (17%) 1 (4%) 2 (9%) 23 (100%)
TOTAL 18 (40%) 9 (20%) 4 (9%) 8 (18%) 4 (9%) 2 (4%) 45 (100%)
Most of the lesions in both groups had a duration of 1 month to 3 months (27% in
apple cider; 52% in salicylic-lactic acid).
B. Cure Rate
Apple Cider Vinegar 5 9% 45 85% 3 6% 53 51%
Salicylic-Lactic Acid 11 22% 39 76% 1 2% 51 49%
TOTAL 16 15% 84 81% 4 4% 104 100%
Not Cured Cured TOTALMissing/ Inc
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 2/12
The cure rate for the apple cider group and salicylic-lactic acid group were 85%
(45/53) and 76% (39/51), respectively. Overall, cure rate for the clinical trial was
81%. Excluding the missing samples, the difference in cure rates were statistically
tested using the Chi-Square Test. There is no sufficient evidence to prove that
there is a significant difference between the cure rates of apple cider treatment and
salicylic-lactic acid treatment (p=0.102).
Intention to Treat Analysis:
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 3/12
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 4/12
Cured
Not Cured
Missing / Lost to ff up
Cure Rates (%):
Complete Case Analysis
Assuming good outcome
Assuming poor outcome
--
--
--
0.102 (not sig. diff)
0.086 (not sig. diff)
0.275 (not sig. diff)
3
90 (45/50)
90.6 (48/53)
84.9 (45/53)
39
11
1
78 (39/50)
78.4 (40/51)
76.5 (39/51)
a cy c- ac c c
(n=51)
- quare es
P-value (vs 0.05)
45
5
Apple Cider (n=53)
Complete case analysis ignores all those who were not able to complete the clinical
trial. Assuming good outcome assumes those that were lost to follow up were cured,
while assuming poor outcome assumes those that were lost to follow up were not
cured. Chi-square test results in all three scenarios show that there is no sufficient
evidence to prove that the cure rates between the apple cider group and the
salicylic-lactic acid group are significantly different.
C. Decrease in Size of Warts
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 5/12
At Day 0 of the clinical trial, the common warts had a mean size of 0.499cm in the
apple cider group and 0.481cm in the salicylic-lactic acid group. For both T-test for
Independent Samples (p=0.296) and Mann-Whitney Test (p=0.425), there is no
sufficient evidence to prove that the mean sizes at Day 0 of the two groups are
significantly different. Therefore, a comparison between the mean size decrease of
the common warts at the end of the clinical trial can be performed.
The size of common warts in the apple cider group decreased by an average of
0.441cm, while the size of the common warts in the salicylic-lactic acid group
decreased by an average of 0.375cm. With p=0.087 resulting from the T-Test for
Independent Samples and with p=0.086 resulting from the Mann-Whitney Test,
there is no sufficient evidence to prove that the mean size decrease between the
two groups are significantly different.
D. Number of Days to Cure
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 6/12
Mean time to cure among apple cider group and among salicylic-lactic acid group
were 20 and 18 days, respectively. There is a significant difference between the two
group’s mean time to cure (p=0.026), with the salicylic-lactic acid group showing abetter clinical response across time.
E. Incidences of Adverse Effects
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 7/12
GROUP * Hypopigmentation Crosstabulation
Count
Hypopigmentation
TotalNO YES
GROUP Apple Cider Vinegar 49 1 50
Salicylic Acid + Lactic Acid 46 4 50
Total 95 5 100
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 8/12
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 9/12
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 10/12
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 11/12
Except for Maceration (p=0.006), there is no significant difference in the incidences
of adverse reaction between the two groups.
In the apple cider group, 2 patients had erythema, 1 had hypopigmentation, 1 had
blisters and another 1 experienced scarring. In the salicylic-lactic acid group, 2 had
erythema, 8 had maceration and 4 had hypopigmentation.
F. Disease Control
Among the apple cider group, 45 (84.9%) out of 53 common warts were cleared or
resolved, 1 (1.9%) common wart showed improved or evident clearance and 4
(7.5%) common warts were partially cleared.
Among the salicylic-lactic acid group, 39 (76.5%) out of 51 common warts were
cleared or resolved, 1 (2.0%) common wart showed improved or evident clearance
and 10 (19.6%) common warts were partially cleared.
Erythem a M acerationHypop igm entationHyperpigm entationBlistering Pain/ Tendern essScarring
Secondary Ba
In fe ct io n O
p le C id e r V in e ga r 2 0 1 0 1 0 1 0
cy lic-Lact ic A cid 2 8 4 0 0 0 0 0
7/30/2019 Apple Cider vs Salicylic-Lactic Acid v2
http://slidepdf.com/reader/full/apple-cider-vs-salicylic-lactic-acid-v2 12/12
66.7% (56/84) of cleared or resolved common warts were already soft on palpation
prior to curettage while 29.8% (25/84) were doughy. Common warts which showed
improved or evident clearance were all already soft. Of those common warts whichwere partially cleared, 71.4% (10/14) were also already soft.