Annotazione
Sequenze Sequenze+
informazioni
Manuale
Automatica
Attendibilità di una sequenza proteica
In ordine decrescente
- Proteina nota- mRNA noto (tradotto?)- Gene noto (splicing
alternativi?)- Gene predetto per omologia con proteine note- Gene predetto con altri metodi
Attendibilità di una funzione
In ordine decrescente- Informazioni ottenute dalla
letteratura con metodi manuali
- Per similarità con proteine a funzione nota
- Con altri metodi bioinformatici
UNIPROT
UNIPROT
TrEMBLSwiss-Prot
5.400.000 350.000
5.750.000 proteine
Annotate manualmente
Annotate automaticamente
Campi di una banca datidi proteine
ProteinaID
ACCESSION
DATA CREAZIONE
DATA MODIFICA
NOME
SINONIMI
NOME GENE
SPECIE
FUNZIONE
SEQUENZA
…
SequenceSequence
MDVPCPWYSLLIPLFVFIFLLIHHCFFTTSKKQNMLLLPSPRKLPIIGNLHQLGSLPHRSLHKLSQKYGPVMLLHFGSKPVIVASSVDAARDIMKTHDVVRDIMKTHDVV
Lenght107 AA
Weight11023 Da
CRC numberNumero di controllo integrità
Es. 0890DD39E1473584
DescriptionProtein name
Annexin A5
SynonymsAnnexin V, Lipocortin V, Endonexin II
Ec numberEC 6.3.5.5
ContainsProdotti di taglio contenuti
IncludesDomini funzionali contenuti
Gene NamePrimary
Phospholipase A2
SynonymcPLA2
Locus nameNome ordinato sul genoma
Es: XAC2464
ORF nameNome temporaneo su genoma
Es: ACAM_3000_MVA_081
OrganismCommon name
Eggplant
ScientificSolanum melongea
SynonymAubergine
ClassificationEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core
eudicotyledons; a sterids; lamiids; Solanales; Solanaceae; Solanum
TaxonomyNCBI_TaxID=9606
DateDD-MMM-YYYY Es. 01-Gen-2001
IntegratedData di creazione
Entry modifiedUltima modifica alla descrizione
Sequence modifiedUltima modifica alla sequenza
References
PositionNuleotide/protein sequence Genomic dna/rna, X-ray crystallography, variant
CommentStrain, Tissue, Plasmid, Species, TransposonEs. STRAIN=Liver
TypeJournal Article, Thesis, Submission, Unpublished
Author, Title, Year, Journal title, VolumeDati bibliografici
Keywords
Es:3D-structure; Alternative splicing; Alzheimer disease; Amyloid;Apoptosis; Cell adhesion;
Coated pits; Copper;Direct protein sequencing; Disease mutation; Endocytosis;Glycoprotein; Heparin- binding; Iron; Metal-binding; Notch signaling pathway; Phosphorylation; Polymorphism; Protease inhibitor; Proteoglycan; Serine protease inhibitor; Signal;Transmembrane; Zinc.
Iron
Bind
OrganelleEs:
HydrogenosomeMitochondrionNucleomorphPlasmidPlastid
CommentsFUNCTION: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. ALLERGEN: Causes an allergic reaction in human. Minor allergen of bovine dander.ALTERNATIVE PRODUCTS: Event=Alternative initiation; Comment=2 isoforms, Alpha and Beta, are produced by alternative initiation;BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=98 uM for ATP; KM=688 uM for pyridoxal; Vmax=1.604 mmol/min/mg enzyme; pH dependence: Optimum pH is 6.0. Active from pH 4.5 to 10.5;CATALYTIC ACTIVITY: ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine. COFACTOR: Pyridoxal phosphate. DEVELOPMENTAL STAGE: Expressed early during conidial (dormant spores) differentiation. DISEASE: Defects in PHKA1 are linked to X-linked muscle glycogenosis [MIM:311870]. It is a disease characterized by slowly progressive, predominantly distal muscle weakness and atrophy. DOMAIN: Contains a coiled-coil domain essential for vesicular transport and a dispensable C-terminal region.PATHWAY: Porphyrin biosynthesis by the C5 pathway; second step. PHARMACEUTICAL: Available under the name Proleukin (Chiron). Used in patients with renal cell carcinoma or metastatic melanoma.POLYMORPHISM: The allelic form of the enzyme with Gln-191 (Allozyme A) hydrolyzes paraoxon with a low turnover number and the one with Arg-191 (Allozyme B) with a high turnover number. PTM: N-glycosylated and probably also O-glycosylated.RNA EDITING: Modified_positions=393, 431, 452, 495.TISSUE SPECIFICITY: Shoots, roots, and cotyledon from dehydrating seedlings.TOXIC DOSE: PD(50) is 1.72 mg/kg by injection in blowfly larvae.
CommentsALLERGEN Information relevant to allergenic proteins
ALTERNATIVE PRODUCTS Description of the existence of related protein sequence(s) produced by alternative splicing of the same gene or by the use of alternative initiation codons; see 3.20.15
BIOPHYSICOCHEMICAL PROPERTIES
Description of the information relevant to biophysical and physicochemical data and information on pH dependence, temperature dependence, kinetic parameters, redox potentials, and maximal absorption; see 3.20.8
BIOTECHNOLOGY Description of the use of a specific protein in a biotechnological process
CATALYTIC ACTIVITY Description of the reaction(s) catalyzed by an enzyme [1]
COFACTOR Description of any non-protein substance required by an enzyme for its catalytic activity
DEVELOPMENTAL STAGE Description of the developmentally-specific expression of mRNA or protein
DISEASE Description of the disease(s) associated with a deficiency of a protein
DOMAIN Description of the domain structure of a protein
ENZYME REGULATION Description of an enzyme regulatory mechanism
FUNCTION General description of the function(s) of a protein
INDUCTION Description of the compound(s) or condition(s) that regulate gene expression
INTERACTION Conveys information relevant to binary protein-protein interaction 3.20.12
MASS SPECTROMETRY Reports the exact molecular weight of a protein or part of a protein as determined by mass spectrometric methods; see 3.20.23
PATHWAY Description of the metabolic pathway(s) with which a protein is associated
PHARMACEUTICAL Description of the use of a protein as a pharmaceutical drug
POLYMORPHISM Description of polymorphism(s)
RNA EDITING Description of any type of RNA editing that leads to one or more amino acid changes
SIMILARITY Description of the similaritie(s) (sequence or structural) of a protein with other proteins
SUBCELLULAR LOCATION Description of the subcellular location of the mature protein
SUBUNIT Description of the quaternary structure of a protein and any kind of interactions with other proteins or protein complexes; except for receptor-ligand interactions, which are described in the topic FUNCTION.
TISSUE SPECIFICITY Description of the tissue-specific expression of mRNA or protein
TOXIC DOSE Description of the lethal dose (LD), paralytic dose (PD) or effective dose of a protein
FeaturesFeature type
Start-End range23-61
DescriptionDipende dal tipo
INIT_MET - Initiator methionine. SIGNAL - Extent of a signal sequence (prepeptide).PROPEP - Extent of a propeptide TRANSIT - Extent of a transit peptide (mitochondrion, chloroplast, thylakoid)CHAIN - Extent of a polypeptide chain in the mature protein. PEPTIDE - Extent of a released active peptide.TOPO_DOM - Topological domain. TRANSMEM - Extent of a transmembrane region.DOMAIN - Specific combination of secondary structures. NON_TER - The residue at an extremity of the sequence is not the terminal residue.REPEAT - Extent of an internal sequence repetition. CA_BIND - Extent of a calcium-binding region.ZN_FING - Extent of a zinc finger region. DNA_BIND - Extent of a DNA-binding reNP_BIND - Extent of a nucleotide phosphate-binding region. REGION - Extent of a region of interest in the sequen Hydrophobic.COILED - Extent of a coiled-coil region. MOTIF - Short (up to 20 amino acids) sequence motif of biological interest.COMPBIAS - Extent of a compositionally biased region. ACT_SITE - Amino acid(s) involved in the activity of an enzyme.METAL - Binding site for a metal ion. BINDING - Binding site for any chemical group (co-enzyme, prosthetic group, etc.).SITE - Any interesting single amino-acid site, not defined. SE_CYS - SelenocysteineMOD_RES - Posttranslational modification of a residue. LIPID - Lipid bindingCARBOHYD - Glycosylation site. DISULFID - Disulfide bond.CROSSLNK - Posttranslationally formed amino acid bonds. VARSPLIC - Description of sequence variants produced by alternative splicing.VARIANT - Authors report that sequence variants exist. MUTAGEN - Site which has been experimentally altered.UNSURE - Uncertainties in the sequence CONFLICT - Different sources report differing sequences.NON_CONS - Non-consecutive residues. HELIX - Secondary structureSTRAND - Secondary structure TURN - Secondary structure
IdentificativiEntry name
Swissprot: Sigla + specie Es. B2MG_HUMAN
TrEMBL: Accessionnumber + specieEs. O95417_MOUSE
Accession NumberEs: Q1AAA9
Primary : Quello da utilizzareSecondary : Elenco dei precedenti
numeri non più usati
Cross references70 Banche dati
Altri Idaltre informazioni
Ididentificativo primario della
bancadati
Es. EMBL; AJ297977; CAC17465.1; -; Genomic_DNA.
Databasenome banca dati