PBA workshop MSU JUNErsquo 10 1
QTL Discovery
amp Perspectives of use of
markers in fruit breeding programs
Content
Introduction
QTL-Discovery
Perspectives for use
Example Nectria (Canker) resistance
Summary
PBA workshop MSU JUNErsquo 10 2
Introduction
QTL discovery
Assessment of correlation trait harr molecular marker
Initial step towards Marker Assisted Breeding Discovery Validation High Throughput markers Logistics
Validation
Reproducibility on multiple families pedigrees
Mode of action
ndash Additive Dominance
ndash Gene x Gene interactions Independent action
Allelic diversity
Relative importance
PBA workshop MSU JUNErsquo 10 3
R R R S S S
Introduction
QTL Discovery
Assessment of correlation trait harr molecular marker
Requirements
1 Trait of interest
2 Phenotypic data
3 Segregating germplasm
4 Marker data genome-wide
5 QTL-mapping software
PBA workshop MSU JUNErsquo 10 4
QTL-Discovery in FruitBreedomics - Peach
1 Fruit related Traits
PBA workshop MSU JUNErsquo 10 5
ndash Maturity time
ndash Fruit Size
ndash Flesh color
ndash Firmsoft melting
ndash Flesh adherence
ndash Titrable acidity
ndash Total sugar
ndash Flowering time
ndash Flowering intensity
ndash White anthers
ndash Monilinia resistance
2 Phenotypic data
Historic
bull EU-Projects
bull National projects
New - FruitBreedomics
3 Segregating germplasm 18 families plusmn1500 individuals
5 breeding programs 2 countries
4 Marker Data 9K array
QTL-Discovery in FruitBreedomics - apple
1 Fruit Quality Traits gt 10
PBA workshop MSU JUNErsquo 10 6
Fruit Quality ndash Taste
bull Firmness bull Juiciness
bull Crispness bull Total Sugar bull Acidity
ndash Storability ndash Shelf life ndash Harvest date ndash Fruit size ndash Skin Color Resistance to Nectria
2 Phenotypic data
HiDRAS
bull 3 Years Multiple time moments
ndash Harvest Storage 2 amp 4M
ndash Shelf Life 2wks - 2M storage
National projects
3 Segregating germplasm 24 families plusmn1600 individuals
6 breeding programs 6 countries
4 Marker Data
Discovery Marker data
20K SNP array
New available 2013 Jan 16
First marker data Jan 18
Great performance
Some SSR
previous projects
2013 Exciting year
PBA workshop MSU JUNErsquo 10 7
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Content
Introduction
QTL-Discovery
Perspectives for use
Example Nectria (Canker) resistance
Summary
PBA workshop MSU JUNErsquo 10 2
Introduction
QTL discovery
Assessment of correlation trait harr molecular marker
Initial step towards Marker Assisted Breeding Discovery Validation High Throughput markers Logistics
Validation
Reproducibility on multiple families pedigrees
Mode of action
ndash Additive Dominance
ndash Gene x Gene interactions Independent action
Allelic diversity
Relative importance
PBA workshop MSU JUNErsquo 10 3
R R R S S S
Introduction
QTL Discovery
Assessment of correlation trait harr molecular marker
Requirements
1 Trait of interest
2 Phenotypic data
3 Segregating germplasm
4 Marker data genome-wide
5 QTL-mapping software
PBA workshop MSU JUNErsquo 10 4
QTL-Discovery in FruitBreedomics - Peach
1 Fruit related Traits
PBA workshop MSU JUNErsquo 10 5
ndash Maturity time
ndash Fruit Size
ndash Flesh color
ndash Firmsoft melting
ndash Flesh adherence
ndash Titrable acidity
ndash Total sugar
ndash Flowering time
ndash Flowering intensity
ndash White anthers
ndash Monilinia resistance
2 Phenotypic data
Historic
bull EU-Projects
bull National projects
New - FruitBreedomics
3 Segregating germplasm 18 families plusmn1500 individuals
5 breeding programs 2 countries
4 Marker Data 9K array
QTL-Discovery in FruitBreedomics - apple
1 Fruit Quality Traits gt 10
PBA workshop MSU JUNErsquo 10 6
Fruit Quality ndash Taste
bull Firmness bull Juiciness
bull Crispness bull Total Sugar bull Acidity
ndash Storability ndash Shelf life ndash Harvest date ndash Fruit size ndash Skin Color Resistance to Nectria
2 Phenotypic data
HiDRAS
bull 3 Years Multiple time moments
ndash Harvest Storage 2 amp 4M
ndash Shelf Life 2wks - 2M storage
National projects
3 Segregating germplasm 24 families plusmn1600 individuals
6 breeding programs 6 countries
4 Marker Data
Discovery Marker data
20K SNP array
New available 2013 Jan 16
First marker data Jan 18
Great performance
Some SSR
previous projects
2013 Exciting year
PBA workshop MSU JUNErsquo 10 7
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Introduction
QTL discovery
Assessment of correlation trait harr molecular marker
Initial step towards Marker Assisted Breeding Discovery Validation High Throughput markers Logistics
Validation
Reproducibility on multiple families pedigrees
Mode of action
ndash Additive Dominance
ndash Gene x Gene interactions Independent action
Allelic diversity
Relative importance
PBA workshop MSU JUNErsquo 10 3
R R R S S S
Introduction
QTL Discovery
Assessment of correlation trait harr molecular marker
Requirements
1 Trait of interest
2 Phenotypic data
3 Segregating germplasm
4 Marker data genome-wide
5 QTL-mapping software
PBA workshop MSU JUNErsquo 10 4
QTL-Discovery in FruitBreedomics - Peach
1 Fruit related Traits
PBA workshop MSU JUNErsquo 10 5
ndash Maturity time
ndash Fruit Size
ndash Flesh color
ndash Firmsoft melting
ndash Flesh adherence
ndash Titrable acidity
ndash Total sugar
ndash Flowering time
ndash Flowering intensity
ndash White anthers
ndash Monilinia resistance
2 Phenotypic data
Historic
bull EU-Projects
bull National projects
New - FruitBreedomics
3 Segregating germplasm 18 families plusmn1500 individuals
5 breeding programs 2 countries
4 Marker Data 9K array
QTL-Discovery in FruitBreedomics - apple
1 Fruit Quality Traits gt 10
PBA workshop MSU JUNErsquo 10 6
Fruit Quality ndash Taste
bull Firmness bull Juiciness
bull Crispness bull Total Sugar bull Acidity
ndash Storability ndash Shelf life ndash Harvest date ndash Fruit size ndash Skin Color Resistance to Nectria
2 Phenotypic data
HiDRAS
bull 3 Years Multiple time moments
ndash Harvest Storage 2 amp 4M
ndash Shelf Life 2wks - 2M storage
National projects
3 Segregating germplasm 24 families plusmn1600 individuals
6 breeding programs 6 countries
4 Marker Data
Discovery Marker data
20K SNP array
New available 2013 Jan 16
First marker data Jan 18
Great performance
Some SSR
previous projects
2013 Exciting year
PBA workshop MSU JUNErsquo 10 7
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Introduction
QTL Discovery
Assessment of correlation trait harr molecular marker
Requirements
1 Trait of interest
2 Phenotypic data
3 Segregating germplasm
4 Marker data genome-wide
5 QTL-mapping software
PBA workshop MSU JUNErsquo 10 4
QTL-Discovery in FruitBreedomics - Peach
1 Fruit related Traits
PBA workshop MSU JUNErsquo 10 5
ndash Maturity time
ndash Fruit Size
ndash Flesh color
ndash Firmsoft melting
ndash Flesh adherence
ndash Titrable acidity
ndash Total sugar
ndash Flowering time
ndash Flowering intensity
ndash White anthers
ndash Monilinia resistance
2 Phenotypic data
Historic
bull EU-Projects
bull National projects
New - FruitBreedomics
3 Segregating germplasm 18 families plusmn1500 individuals
5 breeding programs 2 countries
4 Marker Data 9K array
QTL-Discovery in FruitBreedomics - apple
1 Fruit Quality Traits gt 10
PBA workshop MSU JUNErsquo 10 6
Fruit Quality ndash Taste
bull Firmness bull Juiciness
bull Crispness bull Total Sugar bull Acidity
ndash Storability ndash Shelf life ndash Harvest date ndash Fruit size ndash Skin Color Resistance to Nectria
2 Phenotypic data
HiDRAS
bull 3 Years Multiple time moments
ndash Harvest Storage 2 amp 4M
ndash Shelf Life 2wks - 2M storage
National projects
3 Segregating germplasm 24 families plusmn1600 individuals
6 breeding programs 6 countries
4 Marker Data
Discovery Marker data
20K SNP array
New available 2013 Jan 16
First marker data Jan 18
Great performance
Some SSR
previous projects
2013 Exciting year
PBA workshop MSU JUNErsquo 10 7
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
QTL-Discovery in FruitBreedomics - Peach
1 Fruit related Traits
PBA workshop MSU JUNErsquo 10 5
ndash Maturity time
ndash Fruit Size
ndash Flesh color
ndash Firmsoft melting
ndash Flesh adherence
ndash Titrable acidity
ndash Total sugar
ndash Flowering time
ndash Flowering intensity
ndash White anthers
ndash Monilinia resistance
2 Phenotypic data
Historic
bull EU-Projects
bull National projects
New - FruitBreedomics
3 Segregating germplasm 18 families plusmn1500 individuals
5 breeding programs 2 countries
4 Marker Data 9K array
QTL-Discovery in FruitBreedomics - apple
1 Fruit Quality Traits gt 10
PBA workshop MSU JUNErsquo 10 6
Fruit Quality ndash Taste
bull Firmness bull Juiciness
bull Crispness bull Total Sugar bull Acidity
ndash Storability ndash Shelf life ndash Harvest date ndash Fruit size ndash Skin Color Resistance to Nectria
2 Phenotypic data
HiDRAS
bull 3 Years Multiple time moments
ndash Harvest Storage 2 amp 4M
ndash Shelf Life 2wks - 2M storage
National projects
3 Segregating germplasm 24 families plusmn1600 individuals
6 breeding programs 6 countries
4 Marker Data
Discovery Marker data
20K SNP array
New available 2013 Jan 16
First marker data Jan 18
Great performance
Some SSR
previous projects
2013 Exciting year
PBA workshop MSU JUNErsquo 10 7
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
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E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
QTL-Discovery in FruitBreedomics - apple
1 Fruit Quality Traits gt 10
PBA workshop MSU JUNErsquo 10 6
Fruit Quality ndash Taste
bull Firmness bull Juiciness
bull Crispness bull Total Sugar bull Acidity
ndash Storability ndash Shelf life ndash Harvest date ndash Fruit size ndash Skin Color Resistance to Nectria
2 Phenotypic data
HiDRAS
bull 3 Years Multiple time moments
ndash Harvest Storage 2 amp 4M
ndash Shelf Life 2wks - 2M storage
National projects
3 Segregating germplasm 24 families plusmn1600 individuals
6 breeding programs 6 countries
4 Marker Data
Discovery Marker data
20K SNP array
New available 2013 Jan 16
First marker data Jan 18
Great performance
Some SSR
previous projects
2013 Exciting year
PBA workshop MSU JUNErsquo 10 7
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
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CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
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CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
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E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
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E34M56-220-4+ 588E32M50-400-1+ 604
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E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
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MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
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E34M53-138-10+ 347E33M51-221-5+ 362
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CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
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CH02b12-129141 -+
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CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
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E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
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E34M57-111-15- fD -210
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CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
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582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
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CH04g09-153 +453
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CH02b03b-9397 -+CH01f07a-194190 -+
520
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E34M54-82-17 - 610
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E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
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J11-5
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CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
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E35M52-400-1 +687
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E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
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CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
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E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
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E34M57-111-15- fD -210
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E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
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582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
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E34M54-122-11+ 510
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CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
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CH03d11-121127+179 +- 353
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CH04g09-153 +453
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520
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E31M48-250-8 -676
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E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
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E34M55-410-3- Pf +174
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CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
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CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
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E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
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669
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671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
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CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
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E33M47-275-4 -273
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CH04c07-106108 -+338
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E35M58-95-8 -586
J13 P13 J14 P14
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E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
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E34M57-292-5 + 104
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E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
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E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
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E32M52-264-4+ 341
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517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Discovery Marker data
20K SNP array
New available 2013 Jan 16
First marker data Jan 18
Great performance
Some SSR
previous projects
2013 Exciting year
PBA workshop MSU JUNErsquo 10 7
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Perspectives
PBA workshop MSU JUNErsquo 10 8
Great perspectives bull Many QTL discovered in EU-HiDRAS
bull Large intervals due to low marker density ( individuals high)
25
56
77
00
02
04
1
1
14
24
51
67
00
02
04
2
19
24
43
76
00
02
04
3
2
18
32
55
73
00
02
04
4
8
14
20
34
4547
69
90
100
10700
02
04
5
9
40
78
00
02
04 6
26
35
00
02
04
7
18
33
52
62
00
02
04
8
24
42
59
69
00
02
04
06
9
1314
40
50
64
81
104
00
02
04
10
13
29
45
51
61
76
00
02
04
12
3
18
30
45
64
89
00
02
04
13
8
23
37
77
110
116
00
02
04
15
8
14
30
47
76
00
02
04
16
0
12
2325
35
55
91
100
00
02
04
17
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Validation amp Marker development
On-going within FruitBreedomics
Development of SNP markers (WP1)
Firmness amp Storability
2 genes involved in ethylene pathway
Relevance confirmed in multiple studies
Already in use for MAB
bull WSU Russian programs
PBA workshop MSU JUNErsquo 10 9
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Validation amp Marker development RosBREED
RosBREED project
US USDA-SCRI
Multi State International
Apple Peach Cherry Strawberry
wwwRosBREEDorg
Validation apple
Acidity
bull 2 loci including Ma locus
Crispness Juiciness (one LG)
Skin colour Rf locus
Bitter pit 3 loci
and others
Validation Peach
Acidity
Sweetness
Maturity date
Fruit size
and others
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Perspectives on use in breeding
Discovery great potential
Validation completed for 1st genes
Marker Development started
Logistics started
Enhancement through intercontinental collaborations
RosBREED
Plant amp Food Research
PBA workshop MSU JUNErsquo 10 11
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Nectria Canker
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Introduction on Nectria
PBA workshop MSU JUNErsquo 10 13
Causes canker in apple
Problem in NW-Europe (Humid climates)
Many (popular) cvs extremely susceptible
Braeburn Cox Gala Kanzi Rubens Prima
Fungicides for Scab act also to Nectria
Problems with Nectria may increase
Scab resistance
Susceptible parents
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
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E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
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E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Introduction to Nectria
Breeding for resistance is hard
R ndash highly quantitative
Only few cultivars show reasonable resistance
bull Jonathan Golden Delicious Lombartrsquos Calvillle Santana
Resistance tests are laborious and time-lasting
Availability of markers highly useful
Difficult to develop
PBA workshop MSU JUNErsquo 10 14
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Discovery
Germplasm
Jonathan x Prima
bull Jonathan cv with highest level of resistance
bull Prima highly susceptible chopped down in biological production
PBA workshop MSU JUNErsquo 10 15
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Discovery National Project
Germplasm Jonathan x Prima
PBA workshop MSU JUNErsquo 10 16
Phenotyping
Artificial inoculation (1994)
Greenhouse no 2nd infection
Colonization rate Length of cankers in time (1996)
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Phenotypic data Frequency Distribution
Size of cankers (in mm)
p
roge
ny
0
5
10
15
20
25
30
35
56
4
8
12
16
20
24
28
32
36
40
44
48
52
60
64
68
72
76
1
28
25
16
12
5 4
2 1
13
30
18
8 6
3
1
4 5
3
1 1 1 1 1 1 1
bull Prima Large experimental variation
Jonathan x Prima
Jonathan
Prima
bull JP-Population sub-groups
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5-
438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638CH05g08-175178+- 648
E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M47-128-10- 00
E31M56-185-4+ 65E33M47-113-15-Fd+ 77
E32M58-352-1- 118
E35M47-253-9-Fd- 149E35M61-163-6+ 154
E33M47-290-3+ 197
E35M53-191-4+ 224
MC036c- 267
E31M52-140-14- 490E31M52-139-9- 492E34M54-142-7- 493
E31M52-178-12- 505
E33M60-92-20+ 705
E35M54-347-2 - 00E35M50-215-5 - 28E35M51-154-16-Pf-fD - 34
E33M60-172-11-fD + 75
E34M60-99-12 + 233E34M50-180-7 - 250
E34M48-308-28-fD-309 + 319E34M60-264-4 - 320E34M47-170-6 + 323E34M60-108-11 - E32M58-106-8 -
337
E34M55-278-6-Pf-272 - 412
E35M53-120-7 + 491
E32M52-171-7 -636
E31M48-173-16-Fd+ 00
CH04e02-155148-+ 34
E35M51-198-14- 189E34M47-137-9- 190
E34M50-122-14- 191E31M48-127-20- 201E34M55-317-5- 204
GD162-223247-+ 255E35M52-292-pF-3- 262
CH02h11a-104120-+ 292
E32M50-395-2+ 507
E35M54-206-5- 614
E34M51-250-5- 667
CH04e02-155163 -+00E34M51-100-16 -05E34M59-291-3-Pf +08E33M60-286-6 +15MC036a -23E33M51-207-6-Pf -48
E33M60-148-13 -239
CH01d03-138158 -266E32M48-439-1 -313
CH02h11a-124130 +-314E35M51-88-18 -315E33M51-225-4-Pf-fD +322
E35M61-256-4 -454E34M51-278-3 -473
CH02c02b-112116 +-595
J3 P3 J4 P4
CH03g12z-170154+- 00
E35M50-137-12- 100E35M53-168-6- 122E34M49-199-3- 125
E35M52-133-7+ 265E33M47-97-16+ 274
E35M47-310-4-pF-Fd+ 288
E31M52-90-13-E35M51-305-pF-5- 438
E33M47-76-17- 440E31M52-90-18- 441
E35M47-200-12- 489
E34M59-310-2- 638
CH05g08-175178+- 648E34M51-122-13- 660
MC014d -00
CH03g12z-170162+-191E36M51-145-16-fD -197E36M51-397-3 -223
E35M52-81-11-Pf +334
E33M60-98-18 +494
E31M52-302-4-Pf +564
E35M47-361-1 +645E32M50-169-10 +664
E34M54-244-4 +818E34M51-133-11 +819E34M51-115-14 +824CH05g08-1750 -+839
CH02f06-158150-+ 00E31M48-357-4+ 30
E34M48-171-18-pF-Fd+E34M53-221-5+
36
E35M47-278-8+E35M47-224-10+ 92
E34M55-430-2+ 94
E35M49-163-11+ 342E35M50-210-6+ 369E33M60-321-4+ 370
E34M54-111-12+ 375E32M58-116-6+ 392E34M51-153-8+
E32M47-119-11+ 396
CH05e03-182161-+ 398MC029a- 479
CH03d01-1130-+ 482E34M48-262-10- 487E35M52-89-10- 501
MC064b- 518
E34M53-470-1 - 00
E33M60-96-19 -172E34M47-298-1 -174
E35M55-118-8 -232
E34M59-192-Pf-9 -272
CH05e03-182176+-279
CH03d01-113111-+362
E34M50-110-16 +415
E34M48-288-29 +444
J1 P1 J2 P2
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M53-169-7- 00
MC064a- 64
E36M51-118-19+ 124
E33M51-136-11-fD+ 176
E32M47-107-15+ 298
CH04e05-174201-+ 309E35M58-242-2+
E32M47-110-14+310
E32M48-400-3+ 324E32M48-151-8-Fd+ 330
E35M47-220-11-pF-Fd- 355
E34M56-220-4+ 588E32M50-400-1+ 604
E31M48-89-25+ 734
J7E35M48-81-12 +00
E34M49-96-6 -43MC064a -53E34M50-198-6 +73E31M48-365-3-fD -80E34M50-171-10 -88E35M58-91-9 +120E34M55-283-19 -149E36M51-206-8-Pf-fD +155
E31M48-350-5 +210
E34M47-213-3 +247
E31M48-187-14 +287E35M59-255-1 -299E35M48-226-4 +302E33M60-278-7 -313E35M48-131-9-Pf +349
E35M47-280-7 -394
CH04e05-174209 +-396
E32M58-470-12 +588
P7
E31M49-206-5-Fd- 00E34M56-230-2- 11E32M58-228-3+ 32
E34M60-206-8-Fd+ 53
Ly37c+ 136E31M52-465-1-pF+ 137
E35M54-61-15+ 148MC022a+ 154
E35M61-75-8+ 181
E32M48-109-13-Fd+E32M48-63-16-Fd+
E32M48-60-20+426
J8E34M53-97-14 + 00E34M48-218-31 + 26E34M50-175-9 - 45E33M51-67-18 - 51E34M59-203-8 - 55E34M56-228-3 - 67
E34M48-323-6-Pf - 133Ly37c - 160E31M52-232-8 - 167
MC022a - 203E34M48-318-7 - 206E34M57-157-10 - 233E35M58-132-6 - 248E34M57-179-9 - 265E35M53-177-5 - 288
P8
E34M57-190-8+ 00E34M50-115-15- 28
CH04e03-186196+- 56E34M49-86-7- 66
E31M48-483-1-Fd+ 72
CH03a04-100114+- 108
CH02b12-125127+- 125E32M48-130-11+ 126E35M61-308-2- 129
E34M48-154-19-pF- 132E34M55-59-18-pF+ 136
E31M48-185-15-Fd- 141E34M48-115-25- 143
E31M52-215-6Fd-E34M54-72-18+E34M59-96-14+
145
E35M47-158-14+ 146E33M60-304-5-E33M60-340-3+
147
E33M60-213-9+ 155E34M55-85-14-pF+ 160
E35M50-202-7- 168
GD103-114118+- 171E35M53-230-3- 280E35M49-227-8+ 345
E34M53-138-10+ 347E33M51-221-5+ 362
MC025a- 419E33M60-358-2- 447
CH05e06-0150+- 463E36M51-190-11- 468E31M49-385-2- 483E34M60-207-7- 496E35M59-177-6- 506
CH04e03-184204 +- 00E34M57-352-2 + 47E35M59-103-8 +54E35M54-115-9 -96E34M54-105-13 -E32M50-119-13 -E34M49-316-1 +
97
E32M58-98-9 -E32M58-207-13 -E31M52-376-3 -E33M60-104-16 -E33M60-178-10-fD-179 -
98
E33M51-202-7-Pf -99E35M54-69-13 +100E35M47-126-20 -104E35M49-124-14 -115E35M61-307-3 -124E33M60-131-14 -127
CH02b12-129141 -+
CH03a04-0106 +130
E34M48-550-1 +138E34M49-112-5 +207E34M48-440-3 +213E32M50-145-11 -253E34M59-251-6-Pf -307E32M52-375-2-Pf +355E33M51-127-14-Pf-fD -483E35M49-81-18 -501MC025a -515E34M51-103-15 +549E34M51-406-1 -601
CH05e06-0134 -633
E34M50-292-2 +678
E35M49-397-1 -867
E35M50-185-8 +890
E34M48-141-20- 00
E33M47-223-8- 42MC014c- 47
E35M51-158-15-Fd+ 99
CH03d07-206202- 102MC023a+ 119
E34M55-122-10+ 125
E34M48-220-12+E31M52-113-11+
256
E35M59-150-7+ 272
E34M57-300-3+ 413
CH05a05-198218+- 529
CH03c01-172174-+ 548E34M48-257-11+ 561
CH03d07-206218 -+00
E32M48-185-19 +125
E34M57-122-13-fD +151
E31M49-471-13 -203
CH03d12-124150 +-341
E34M57-293-4 +482E34M55-84-16-Pf +495
CH05a05-198218 -+613
CH03c01-172174 +-633
J5 P5 J6 P6
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E34M59-155-10-Fd- 00
E35M49-308-6- 68
E35M55-171-7+ 94E36M51-196-10- 115
E34M53-52-18- 235E32M52-433-1- 241
CH05c07-141149+- 283
E31M51-247-2- 431E35M47-322-3- 455
E36M51-480-1- pF- 464E34M48-83-24+ 466E35M58-174-4+E34M60-257-5+
467
E32M50-90-15- Fd+ 473
CH01h02b-236248+- 491E34M48-103-23- 521
J9-5
E35M61-92-7 + 00
E34M57-102-16 +22
E34M51-123-12-Fd + 76E34M47-267-2 -93
E34M57-111-15- fD -210
E33M60-357-1- fD -248E36M51-167-13-Pf -254
E35M49-155-12 -288E35M47-490-22 -307
CH05c07-141149 -+386
E34M53-150-9 +494E32M52-239-6
+504
E32M52-242-5 +565E32M48-132-10 +567E35M50-380-1 +579E35M47-480-21 +E31M48-132-19 -
582
E31M49-303-3 - 596E32M47-169-7 + 608E32M48-119-12 -618
CH01h02b-236246 +- 655
P9-3
E34M54-206-5-Fd+ 00E32M48-80-15+ 01
E34M50-156-11-Fd+ 06E34M53-120-11- 40
CH04c06z-157169-+ 46
CH02b07-202180-+ 57E34M47-100-13- 58
E34M59-90-15- 62E35M49-419-19- 70E35M47-106-24+
E35M58-113-7+89
CH02a08-151-+ 91E34M51-310-2+ 113
E36M51-102-21+ 118E35M59-220-3+ 128
E31M48-102-22-Fd+ 131
CH02a10-177- 132E32M47-228-1+ 151
E34M59-109-13- 152CH02c11-231237-+ 198
E35M53-357-2+
CH04g09-145-
320
COL-230232+- 475
E34M54-122-11+ 510
CH02b03b-9799t1=-+CH01f07a-194196-+ 559
GD100-236+ 618E35M58-251-1- 634
J10-4
CH04c06z-157169 +- 00E34M50-300-1 -
CH02b07-184202 +- 01
CH02a08-147151 +- 79
E31M48-66-26 -139E35M52-243-4 - 145
CH02a10-149155 +- 177E32M47-126-9 - 193E34M59-314-1-Pf - 195
CH02c11-231235 -+ 293E34M47-105-11 - 309
E32M47-171-6 - 352
CH03d11-121127+179 +- 353
E32M48-212-7 +409E35M52-224-5-Pf -426
CH04g09-153 +453
E35M48-159-7-Pf + 476
CH02b03b-9397 -+CH01f07a-194190 -+
520
COL-220234 +- 545
E34M54-82-17 - 610
E31M48-250-8 -676
P10-5
E33M60-270-8- 00
E35M47-177-13+ 53
E32M48-242-6+ 80E31M49-214-4- 128
CH03d08-228+- 136
CH03d02-203+- 137E35M52-117-8+ 140
CH04a12-180195-+ 253
E33M51-182-9- 302E35M54-67-14-E35M52-375-2-
311
E33M47-115-14- 338E35M52-171-6- 348E34M53-69-16- 355E34M53-360-2- 387
E34M59-68-17- 444
E34M53-107-13- Fd+ 514
E35M59-182-5+ 560
J11-5
E34M59-139-11- Pf +00
E34M54-165-8 +39
E34M50-147-12 +88E31M49-169-7 +89
E34M55-410-3- Pf +174
E34M57-77-19- fD -217
CH04a12-172176 +-286E32M48-287-5 -324E33M51-96-16 -325E32M47-115-13 -329
E32M48-339-4 -375
E34M56-187-5 -417E33M51-133-13- Pf -432E34M55-83-15 -451
E31M49-388-1 -555E34M57-148-11 -559E32M50-189-6 -575
E34M60-410-1 -628
E35M52-400-1 +687
E34M53-228-3 +716E34M55-91-13- Pf +733E34M54-97-14 -752
E35M59-187-4 -808E34M59-260-4 +836E31M48-338-6- fD -846E35M61-384-1 -852
P11-4
CH05d04-174180-+ 00MC032b- 23MC225a- 31
CH04g04-180168-+ 64
E35M48-291-3+ 99E34M51-145-9- Fd+ 100
E33M51-236-3+ 170E34M54-87-16+ 180
CH01g12-110186+- 193E31M52-292-2- 200
E31M52-290-5- pF-Fd- 204
CH04d02-154+- 263
E34M53-159-8+ 294
E35M59-76-9- 435
E34M48-173-17+ 482
J12-4
CH05d04-176186 -+00MC225a +03E32M48-58-17 +04MC032b +07E31M48-248-9 +15E33M47-143-11 -34
CH04g04-180172 -+62E32M58-83-11- fD +82E34M57-117-14- fD +134E35M48-196188-6- Pf -147E34M51-144-10- fD +149E34M60-79-13 -184
E31M48-119-21 -259
E34M53-417-19 - 297
E32M50-202-4 -557
E35M49-127-13 +633E35M47-127-19- fD +634E32M58-224-4 +659
P12-5
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
E31M48-150-18 -796
E32M58-316-2- 00
CH02g01-217+ 09E31M52-127-15- 28E31M52-127-10- 36E34M51-164-6- 43
E32M52-120-9- 133
E31M48-100-23+ 267E34M51-159-7+ 270
E33M47-140-12+ 316
E34M53-191-6 +00
E34M48-283-8 +52E32M58-92-10 +58E34M59-255-5-Pf +72
CH02g01-0226 -91
E34M60-272-3 -265
E35M54-137-8 -357
E31M48-165-17 +507
E35M50-219-4 -E31M49-139-8 -627
E35M54-207-6 -630
CH03h03-91103 +-634E33M47-348-2 -666
CH05f04-162158 +-E34M59-118-12 -
669
E35M58-134-5 -E31M52-158-8 -E33M47-165-10 -E34M48-419-4-fD -
671
E31M52-158-13 -677
E34M48-275-9- 00
E31M56-328-1- 39E32M47-209-3+ 50
E34M48-182-15-pF-Fd- 102E34M50-265-3- 110
E34M55-106-12- 207E31M52-75-14- 220
CH05g11-243233+- 227
CH04c07-112114-+ 246
CH05d03-158173+- 330E31M56-188-3+ 333
E34M57-250-7-Fd+ 362E34M60-290-2a-Fd+ 378
E34M54-340-19- 418E34M55-167-8+ 431E34M53-225-4- 436
E32M52-148-8- 469
E31M48-228-10 -00
E31M48-195-12 -109
CH04f06-175178 -+ 136E34M48-179-16-Pf +151E32M47-92-19 +161E33M51-112-15 +167E33M47-172-9 +197
E33M47-275-4 -273
CH05g11-2430 +-328
CH04c07-106108 -+338
CH05d03-152179 -+432E34M60-247-6 +449E34M49-170-4 -456E35M47-149-16-Pf-fD +473E34M55-166-9 +478
E35M58-95-8 -586
J13 P13 J14 P14
+-
+-
E34M50-177-8- 00
CH05c06-104118 163E35M47-287-6- 171
CH05e04a-159167 186
LY05b- 224
E32M52-264-4+ 341
E31M48-435-2- 420E32M47-129-8+ 466E34M47-198-4+ 467E31M52-52-20+E32M58-113-7+
517
E35M53-91-8+ 556E34M48-122-22+ 565E32M48-51-18+ 569
E32M52-114b-10-pF-E34M59-243-7-
570
E35M54-388-16- 571E35M61-218-5+ 573
CH05e04a-1590 -+00
CH05c06-104110 -+ 02E35M50-99-15 + 34
E34M57-292-5 + 104
E33M47-357-1 - 218
E31M49-81-9 + 260
CH05a04-163183 +- 278
E31M49-480-14 -480E31M51-257-1 +490E31M52-218-5 -562E34M54-200-6 -598E32M58-176-5-fD +615E34M50-101-17 -616E35M59-228-2 -E32M52-113-12-Pf -
637
E35M49-108-16 +658E32M52-330-3-Pf +659E34M51-276-4 -670E34M48-140-21 +E34M48-138-32 +674
E33M60-107-15-fD +679
E34M48-130-27 -790
J16 P16
Marker data
AFLP amp SSR
1998-2000
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
QTL ndashmapping
PBA workshop MSU JUNErsquo 10 19
Effect QTL
Additive
Q1+Q2 -gt Cankers as small as for Jonathan
bull Prima 47 mm
bull Jonathan 23 mm
bull JxP_Q1Q2 23 mm
Highly effective
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
3 QTL identified
2x Jonathan 1x Prima
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Fruitbreedomics
PBA workshop MSU JUNErsquo 10 20
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
P-Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
J-Q1
Position
J-Q1
bull No markers for distal part
bull Sub-optimum
J-Q2
bull 1 marker 35 cM
bull Position
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
J01 J02 J03 J04 J05 J06 J07 J08 J09 J10 J11 J12 J13 J14 J15 J16 J17
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
PBA workshop MSU JUNErsquo 10 21
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bull SNP-array plusmn8000 markers for JxP
bull plusmn3000 for Jonathan
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Fruitbreedomics
Progresses on these historic data
Fine-mapping
Increase marker density
Validation
Reproducibility 2nd mapping population
Extension
Stability over environments
bull Phenotype JxP in Sweden
Other parameters for resistance
bull Infection percentage
Larisa Gustavsson
Hilde Nybom
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23
Summary
Title QTL Discovery amp Perspectives of use of markers in fruit breeding programs
QTL Discovery 1st step to application
Discovery Validation High Throughput markers Logistics
Assessment of correlation trait harr molecular marker
Potential - couple of years
Apple gt 10 fruit quality traits + Nectria resistance
Peach gt Flowering + Fruit traits + Monilinia resistance
Potential - short term (from building on other projects)
Apple Fruit firmness (ACS ACO) Skin Color (Rf) Acidity (Ma) Bitter pit
Peach Sub-acidAcid Flatround (PeachNectarine Yellowwhite flesh)
Pipeline-Logistics under development
PBA workshop MSU JUNErsquo 10 23