(-477

to-

751

)(A) ATG5

(-211

0 to

-314

0)

LT

RCpG

isla

nd

(+ 5

68 t

o -1

10)

S

INE

Supplemental Figure 3

(B) AIM1

CpG

isla

nd

(-980

to

-136

0)

CpG isla

nd

(+30

to +

380)

CpG isla

nd

(+61

0 to

+97

0)

Set 1 2 3

1.HANK12.NK923.YT4.SNK15.M.SssI treated lymphocyte DNA6.Bisulfite treated lymphocyte DNA 7.No template8.NKYS9.KHYGI10.SNK611.KAI312.M.SssI treated lymphocyte DNA13.Bisulfite treated lymphocyte DNA 14.No template

Methylation Specific PCR

400 300

300 200

400 300

1 2 3 4 5 6 7

8 9 10 11 12 13 14

1 2 3 4 5 6 7

8 9 10 11 12 13 14

Set 1

Set 2

Set 3

DN

A lad

der

Met

UnM

Met

UnM

Met

UnM

Met

UnM Met

UnM

Met

UnM

Met

UnM

1 2 3 4 5 6 7

8 9 10 11 12 13 14

Methylation Specific PCR

DN

A la

dder

SINE

1 2 3 4 5 6

8 9 10 11 12 13

300 200

LTR400

3001 2 3 4 5 6 7

8 9 10 11 12 13 14

Met

UnM

Met

UnM

Met

UnM

Met

UnM

Met

UnM

Met

UnM

Met

UnM

1 2 3 4 5 6 7

8 9 10 11 12 13 14