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ZILOSHamilton Thorne Biosciences, Inc.
ZonaInfraredLaser OpticalSystem
ZILOSBovine Oocyte: Drill Hole Sizes
Power: 140 mWDuration: Variable
Diameter: 82 µmCR NA 0.6 a = 3 µm
1.0
1.5
2.0
2.5 3.0
4.0
6.0
4.5
5.0
3.5
0.5
5.5
173 µm
ZILOS
P = 100 milliwatts
Pulse Length = 0.1 ms
Peak Isotherms: 0.1 msec
ZILOS
P = 100 milliwatts Pulse Length = 3 ms
Peak Isotherms + Embryo
ZILOSBovine and Mouse Zona Penetration
For Various Pulse Length, Radial Position Power: 100 mWBeam Focal Radius: 3 µm
3 Bovine Oocytes2 Mouse Embryos
ZILOSPeak Temperatures + Zona Drilled Radius
Laser Power @ Target: 140 mWλ = 1480 nmBeam Diameter: 4μmInitial Temp = 37°C
Isotherms of maximum temperature reached during pulse plotted vs. pulse duration, for laser power at target 140 mW. Max error: 0.5%
Radius of hole drilled in embryo Zona Pellucida (courtesy S. Lanzendorf, Jones Inst.) is also shown.
Number of embryos per measurement indicated above SD error bar.
CONCLUSION: Drilled radii fall closely along the 140°C peak-temperature isotherm.
ZILOSCRI & Other Lasers and Embryo Temp.
Laser Heating of CellPulse: 25.0 ms, 45 mWBeam: 1480 nm, r = 2 μmMax Temperature shown
ZILOSLaser and Embryo Temperature
Laser Heating of CellPulse: 3.0 ms, 140 mWBeam: 1480 nm, r = 2 μmMax Temperature shown
ZILOSZILOS Laser and Embryo Temperature
Laser Heating of CellPulse: 1.0 ms, 140 mWBeam: 1480 nm, r = 2 μmMax Temperature shown
ZILOSClass I - Class IIIb Boundary [FDA 1999]
Laser: λ = 1480 nm
Source: Code of Federal Regulations, 21 CFR 1040.10, 596-614, Table I and IV. 4-1-99 ed.
ZILOSNew Feature – Isotherm Rings
• Shows temperature generation right on the screen
Laser
140°C (hole size)
100°C
80°C
60°C
50°C
3 ms, 140 mW
ZILOSNew Feature – Isotherm Rings
• Real-time feedback eliminates temperature guesswork
3 ms Pulse, 140 mW at target 1 ms Pulse, 140 mW at target
ZILOSIsotherm Rings and Drill Hole Size
ZILOSMore New ZILOS Features
• Enhanced laser, shorter pulses– 1 – 6 millisec– Minimum thermal effects
• Simplified & precise laser alignment
• New control panel and intuitive setup screens
• Multi-pulse firing capability
• Output data to ASCII file
• Hoffman option
• Remote foot/hand switch option
