Application Note

ZEISS LSM 800 and LSM 880 with AiryscanImaging Biological Samples with LSM 800, LSM 880 and Airyscan

Application Note

2

4. Airy disk 5. Airyscan detector

1. Mirror2. Emission filters3. Zoom optics

1

2

3

4 5

4

Figure 1 Beampath Airyscan

4. Airy disk 5. Airyscan detector

1. Mirror2. Emission filters3. Zoom optics

1

2

3

4 5

4

Figure 2 Airyscan in Fast Mode

ZEISS LSM 800 and LSM 880 with AiryscanImaging Biological Samples with LSM 800, LSM 880 and Airyscan

Author: Dr. Annette Bergter Carl Zeiss Microscopy GmbH, Germany

Date: Febuary 2018

Introduction

The Confocal Laser Scanning Microscope (LSM) has become

one of the most popular instruments for fluorescence

imaging in biomedical research, because it affords research-

ers images with high contrast and a versatile optical section-

ing capability to investigate three dimensional biological

structures [1].

The optical sectioning ability of an LSM is a product of

scanning a focused laser spot, across a sample to create an

image one point at a time. The generated fluorescence from

each point is collected by the imaging objective and results

from fluorophores in the sample that reside both in the

desired plane of focus and in out of focus planes. In order to

segregate the fluorescence emitted from the desired focal

plane, an aperture (pinhole) is positioned in the light path to

block all out of focus light from reaching the detector

(traditionally a PMT) [2].

1 Non- inclusive

This conventional scheme of the LSM beampath forced the

user to compromise either on resolution or sensitivity. Reso-

lution is increased by closing the pinhole, at the same time

limiting the amount of light that is allowed to pass through

to the detector. The Airyscan detector of the LSM 880 and

LSM 800 overcomes this challenge. The detector consists of

32 GaAsP PMT detector elements, which are arranged in a

hexagonal array (Figure 1), positioned at a conjugated focal

plane in the beam path the detector is functioning as the

traditional LSM pinhole. This design makes it possible to col-

lect more light (equivalent to a pinhole opened to 1.25 AU),

whilst at the same time dramatically enhancing the resolu-

tion, with every detector element acting as an efficient

pinhole with a diameter of only 0.2 Airy Unit (AU). Instead of

facing an either / or decision, a simultaneous enhancement

of sensitivity and resolution was introduced to LSM imaging.

Airyscan allows for an achievable lateral resolution of 120 nm

Application Note

3

0

100

200

300

400

500

600

700

800

900

1000

2014 2015 2016 2017

No

of P

ublic

atio

ns

LSM 880

LSM 800

Airyscan

Figure 3 Number of publications per year, mentioning LSM 880, LSM 800 or Airyscan. Source: google scholar search engine.

Cell culture / In vitro / Fixed cells

even for single images, and resolves structures of 350 nm

axially when z-stacks are acquired. Detailed descriptions of

the theory and technology of Airyscanning can be found in

these technology notes [3, 4, 5, 6].

As an area detector the Airyscan can capture spatial informa-

tion that is utilized to parallelize the scanning process, col-

lecting 4 image lines simultaneously in the Fast mode (Fig-

ure 2). This means enhancing acquisition speed by a factor

of 4 while keeping high pixel dwell times to efficiently collect

emitted photons. Ordinarily, the focused laser beam is

moved along the x-axis to acquire one image line, before it

is moved in the y-axis to acquire the consecutive image line.

In Fast mode imaging, four image lines are acquired at the

same time when moving the laser in the x-direction.

This publication list1 assembles some of the work that has

been done with LSM 880 and 800 systems (Figure 3). The

great variety of applications collectively profits from the light

efficient beam path of the LSM systems, and the unique

combination of superresolution, high sensitivity and high

speed imaging provided by Airyscan.

Learn more about LSM 800, click here

Learn more about LSM 880, click here

Publication Journal Sample Application System

2018. Maass et al. Spatiotemporal allele organization by allelespecific CRISPR live-cell imaging (SNP-CLING).doi:10.1038/s41594-017-0015-3

NatureStructural & molecular biology

hTERT RPE-1 cellsC28/I2 chondrocytes129S1-SvImJ× CAST-EiJ hybrid 2–3 mESCs129S1/CAST MEFs

Live cell imaging (with Airyscan R-S mode)CRISPR

LSM 880Airyscan

2017. Mogessie & Schuh. Actin protects mammalian eggs against chromosome segregation errors.doi:10.1126/science.aal1647

Science MouseOocytes

Live cell imaging PhotomanipulationFluorescent Immuno-histochemical staining

LSM 880LSM 800AiryscanLSM 710LSM 780

2017. Santisteban et al. Rapid spheroid clearing on a microfluidic chip. doi:10.1039/C7LC01114H

Lab on a Chip Spheroids Clearing, CLARITYFluorescent Immuno-histochemical staining

LSM 880 NLOAiryscan Fast

2017. Kolossov et al. Airyscan super-resolution micros-copy of mitochondrial morphology and dynamics in living tumor cells.doi:10.1002/jemt.22968

Microscopy Research & Technique

U87 glioblastoma cellsA549 lung cancer cells

Live cell imaging LSM 880Airyscan Fast

2017. Korobchevskaya et al. Exploring the Potential of Airyscan Microscopy for Live Cell Imaging.doi:10.3390/photonics4030041

Photonics HeLa cellsRat Basophilic Leukaemia cell (RBL)

Live cell imagingFluorescent Immuno-histochemical staining

LSM 880Airyscan

2017. Galaternik et al. A novel perivascular cell population in the zebrafish brain. doi:10.7554/eLife.24369

eLife Zebrafish Fluorescent Immuno-histochemical stainingKaede photoconversion

LSM 880Airyscan UV Laser

2017. Faris et al. Cellular prion protein is present in mitochondria of healthy mice.doi:10.1038/srep41556

nature Mouse Fluorescent Immuno-histochemical staining

LSM 880Airyscan

Application Note

4

Cell culture / In vitro / Fixed cells

Publication Journal Sample Application System

2017. Ishikawa-Ankerhold et al. Actin-Interacting Protein 1 Contributes to Intranuclear Rod Assembly in Dictyo-stelium discoideum. doi:10.1038/srep40310

nature Dictyostelium discoideum Live cell imagingFRAPFluorescent Immuno-histochemical staining

LSM 880Airyscan

2017. Palma-Vera et al. An air-liquid interphase approach for modeling the early embryo-maternal contact zone.doi:10.1038/srep42298

nature Mammalian oviduct epithelial cells

Fluorescent Immuno- histochemical staining

LSM 800

2017. Yan Chen et al. 12-O-Tetradecanoylphorbol-13-acetate (TPA) is anti-tumorigenic in liver cancer cells via inhibiting YAP through AMOT.doi:10.1038/srep44940

nature Bel-7402 and Bel-7404 Fluorescent Immuno-histochemical staining

LSM 800

2017. Mahadevan et al. Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming.doi:10.1038/srep44667

nature Oocytes Fluorescent Immuno-histochemical staining

LSM 880Airyscan

2017. Kruusvee et al. Structure of the MeCP2–TBLR1 complex reveals a molecular basis for Rett syndrome and related disorders.doi/10.1073/pnas.1700731114

PNAS NIH 3T3 cells Fluorescent Immuno-histochemical stainingCo-localization Assay

LSM 880Airyscan

2017. Bruun et al. Re-evaluating the roles of myosin 18Aα and F-actin in determining Golgi morphology. doi:10.1002/cm.21364

Cytoskeleton CellsGolgi morphology

Live cell imaging LSM 880Airyscan Fast

2017. Want et al. Distinct Neural Properties in the Low Frequency Region of the Chicken CochlearNucleus Magnocellularisdoi:10.1523/ENEURO.0016-17.2017

eNeuron Chicken Fluorescent Immuno-histochemical staining

LSM 880

2017. Tang et al. Disruption of actin motor function due to MoMyo5 mutation impairs host penetration and pathogenicity in Magnaporthe oryzae.doi:10.1111/mpp.12554

Molecular Plant Pathology Magnaporthe oryzae Live cell imagingFRAP

LSM 880

2017. Yanagida et al. Size-selective opening of the blood–brain barrier by targeting endothelial sphingosine 1–phosphate receptor 1doi:10.1073/pnas.1618659114

PNAS Mouse Fluorescent Immuno-histochemical staining

LSM 800

2017. Burda et al. Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress.doi:10.1128/mBio.00139-17

mBio Primary mouse hepatocytes Live cell imaging LSM 880Airyscan LSM 5 Duo

2017. Lei et al. Desacetylvinblastine Monohydrazide Disrupts Tumor Vessels by Promoting VE-cadherin Internalization.doi:10.7150/thno.22222

Theranostics RatHepG2 hepatocellular carcinoma cells

Fluorescent Immuno-histochemical staining

LSM 800

2017. Lee et al. Bio-artificial tongue with tongue extracellular matrix and primary taste cells.doi:10.1016/j.biomaterials.2017.10.019

Biomaterials Primary taste cell-based artificial tongue

Fluorescent Immuno-histochemical staining

LSM 880

2017. Beaudet et al. Active Ran regulates anillin function during cytokinesis.doi:10.1091/mbc.E17-04-0253

Molecular Biology of the Cell

HeLa BHK (Baby Hamster Kidney)tsBN2

Fluorescent Immuno-histochemical staining

LSM 800Airyscan

2017. Takeda et al. A homeostatic apical microtubule network shortens cells for epithelial folding via a basal polarity shift.doi:10.1038/s41556-017-0001-3

NatureCell biology

Drosophila melanogasterEmbryo

Fluorescent Immuno-histochemical staining

Airyscan

2017. Johnson et al. Homeostatic plasticity shapes the visual system’s first synapse.doi:10.1038/s41467-017-01332-7

NatureCommunications

Mouse Fluorescent Immuno-histochemical staining

LSM 880Airyscan

Application Note

5

Cell culture / In vitro / Fixed cells

Publication Journal Sample Application System

2017. Haddad & Couture. Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats.doi:10.3389/fphys.2017.00861

Frontiers in Physiology Rat Fluorescent Immuno-histochemical staining

LSM 800

2017. Gutierrez et al. Encapsulation of curcumin in polymeric nanoparticles for antimicrobial Photodynamic Therapy.doi:10.1371/journal.pone.0187418

PLOS, One Oral biofilm Live cell imaging LSM 800Airyscan

2017. Chiang et al. ADP-ribosylation factor-like 4C Binding to Filamin-A Modulates Filopodium Formation and Cell Migration.doi:10.1091/mbc.E17-01-0059

Molecular Biology of the Cell

HeLa Fluorescent Immuno-histochemical stainingCo-localization

LSM 880Airyscan

2017. Jourdeuil & Taneyhill. Spatiotemporal expression pattern of Connexin 43 during early chick embryo-genesis.doi:10.1016/j.gep.2017.11.001

Gene Expression Patterns Chickenembryo

Fluorescent Immuno- histochemical staining

LSM 800

2017. Norris et al. Toddler signaling regulates meso-dermal cell migration downstream of Nodal signaling. doi:10.7554/eLife.22626

eLife Zebrafish Fluorescent Immuno- histochemical stainingIn vivo imaging

LSM 880Lightsheet Z.1

2017. Martiskainen et al. DHCR24 exerts neuroprotec-tion upon inflammation-induced neuronal death.doi:10.1186/s12974-017-0991-6

Journal of Neuro-inflammation

MouseHippocampal dendritic spines

Fluorescent Immuno- histochemical staining

LSM 800

2017. DeLay et al. Tissue-specific gene inactivation in Xenopus laevis: knockout of lhx1 in the kidney with CRISPR/Cas9. doi:10.1534/genetics.117.300468

Genetics Xenopus laevisembryo

Fluorescent Immuno- histochemical staining

LSM 800Airyscan

2017. Zhang et al. Effect of Selected Plant Growth Regulators on Yield and Stem Height of Spring Wheat in Ontario.doi:10.5539/jas.v9n12p30

Journal of Agricultural Science

Wheat Fluorescent Immuno- histochemical staining

LSM 800

2017. Nagarajan et al. Differential signaling pathways are initiated in macrophages during infection depending on the intracellular fate of Chlamydia spp.doi:10.1111/imcb.1033

Immunology & Cell Biology

MouseChlamydia muridarum Chlamydia caviae

LSM 880

2017. Trøstrup et al. Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor.doi: 10.1111/iwj.12846

International Would Journal

MousePseudomonas aeruginosa

Fluorescent Immuno- histochemical staining

LSM 880

2017. Contreras et al. Vaccinomics Approach to the Identification of Candidate Protective Antigens for the Control of Tick Vector Infestations and Anaplasma pha-gocytophilum Infection.doi:10.3389/fcimb.2017.00360

Frontiers in cellular and infection microbiology

Ixodes scapularisAnaplasma phagocyto-philum

Fluorescent Immuno- histochemical staining

LSM 800

2017.Mina et al. Functional and phylogenetic evidence of a bacterial origin for the first enzyme in sphingoli-pid biosynthesis in a phylum of eukaryotic protozoan parasites.doi:10.1074/jbc.M117.792374

JBC Toxoplasma gondii Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2017. Bekier II et al. Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.doi: 10.1091/mbc.E17-02-0112

Molecular Biology of the Cell

HeLa cellsHEK293 cells

CRISPRFluorescent Immuno- histochemical staining

LSM 880Airyscan

2017. Cosenza et al. Asymmetric centriole numbers at spindle poles cause chromosome missegregation in cancer. doi:10.1016/j.celrep.2017.08.005

Cell Reports GFP-PLK4-U2OS cellsHAPLK4-Dendra-centrin2-HeLa cells

Fluorescent Immuno- histochemical stainingCLEM/FIB-SEMLive cell imaging

LSM 880AiryscanAuriga 60 FIB-SEM

Application Note

6

Cell culture / In vitro / Fixed cells

Publication Journal Sample Application System

2017. Goldie et al. miRNA enriched in human neuroblast nuclei bind the MAZ transcription factor and their precursors con the MAZ consensus motif. doi:10.3389/fnmol.2017.00259

Frontiers in Molecular Neuroscience

SH-SY5Y cells miRNA FISH LSM 800

2017. Dopeso-Reyes et al. Glucocerebrosidase expressi-on patterns in the non-human primate brain. doi:10.1007/s00429-017-1504-1

Brain Structure and Function

Macaca fascicularisBrain sections

Fluorescent Immuno- histochemical staining

LSM 800

2017. Lee et al. Pathogenic mutation in the ALS/FTD gene, CCNF, causes elevated Lys48-linked ubiquitylation and defective autophagy. doi: 10.1007/s00018-017-2632-8

Cellular and Molecular Life Sciences

Neuro-2ASH-SY5Y cells

Fluorescent Immuno- histochemical staining

LSM 880

2017. Simhardri et al. Dysregulation of LIMK-1/cofilin-1 pathway: Apossible basis of alteration of neuronal morphology in experimental cerebral malaria. doi:10.1002/ana.25028

Annals of Neurology Mouse Fluorescent Immuno- histochemical staining

LSM 880

2017 Rist & Thum. A map of sensilla and neurons in the taste system of drosophila larvae.doi:10.1002/cne.24308

JCNJournal of Comparative Neurology

Drosophila melanogaster larvae

Fluorescent Immuno- histochemical staining

LSM 880

2016. Petersen et al. Kinetic disruption of lipid rafts is a mechanosensor for phospholipase D.doi:10.1038/ncomms13873

NatureCommunications

C2C12 cells Fluorescent Immuno- histochemical staining

AiryscanElyra PS.1

2016. Cortese et al. Parkin deficiency reduces hippo-campal glutamatergic neurotransmission by impairing AMPA receptor endocitosis. doi:10.1523/JNEUROSCI.1473-16.2016

JNeurosci Rat cultured neurons Fluorescent Immuno- histochemical staining

LSM 800

2016. Dawar et al. Impaired hematopoietic stem cell differentiation and enhanced skewing towards myeloid progenitors in aged aspase-2-deficient mice. doi:10.1038/cddis.2016.406

NatureCell Death & Disease

Primary BM cells Fluorescent Immuno- histochemical staining

LSM 800

2016. Guardia et al. BORC functions upstream of kinesins 1 and 3 to coordinate regional movement of lysosomes along different microtubule tracks. doi:10.1016/j.celrep.2016.10.062

Cell Reports HeLa Cells Fluorescent Immuno- histochemical stainingColocalization

LSM 780Airyscan

2016. Pifer et al. Grainyhead-like-2 inhibits the co-activator p300, suppressing tubulogenesis and the epithelial-mesenchymal transition. doi:10.1091/mbc.E16-04-0249

Molecular Biology of the Cell

293 HEK cells w/o T antigenMDCK cellsHT1080 cells

Fluorescent Immuno- histochemical staining

LSM 710Airyscan

2016. Tanaka et al. The molecular motor KIF1A trans-ports the TrkA Neurotrophin receptor and is essential for sensory neuron survival and function. doi:10.1016/j.neuron.2016.05.002

Neuron Kif1a conditional knockout miceTRPV1-F11 cells

Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. M. von Nordheim et al. Cutthroat Trout Virus—Towards a Virus Model to Support Hepatitis E Research. doi:10.3390/v8100289

Viruses rainbow trout gill (RTGill-W1) cell linerainbow trout liver (RTL-W1) cell line

Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. C.A. Casey et al. Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. doi:10.1111/acer.13247

AlcoholismClinical and Experimental Research

HepG2 cells transfected with mouse ADH1 (VA-13 cells)Rat hepatocytesRat liver tissue sections

Fluorescent Immuno- histochemical staining

LSM 800Airyscan

2016. S.G.B. Furness et al. A novel ligand of calcitonin receptor reveals a potential new sensor that modulates programmed cell death. doi:10.1038/cddiscovery.2016.62

Nature - Cell Death Discovery

MG 63 (Human osteosarco-ma cell line)A172 (Human glioblastoma cell line) GBM-L2 ( high-grade glioma cell line)

Fluorescent Immuno- histochemical staining

LSM 800Airyscan

Application Note

7

Cell culture / In vitro / Fixed cells

Publication Journal Sample Application System

2016. P. Robinson et al. Detyrosinated microtubules buckle and bear load in contracting cardiomyocytes. doi:10.1126/science.aaf0659

Science Cardiomyocyte cells Live cell imaging LSM 880Airyscan

2016. A. M. Ward et al. The Golgi associated ERI3 is a Flavivirus host factor. doi:10.1038/srep34379

NatureScientific Reports

HuH-7 cells Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. L. Ma et al. Exploring functional roles of TRPV1 intracellular domains with unstructured peptide-insertion screening. doi:10.1038/srep33827

NatureScientific Reports

Mouse TRPV1 cell line Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. E. Sosa et al. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. doi:10.1016/j.scr.2016.09.015

Stem Cell Research riPSC89 cells Fluorescent Immuno- histochemical staining

LSM 880

2016. O. Pylypenko et al. Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes. doi:10.7554/eLife.17523

eLIFE HEK293 cellsWild-type C57BL/6

FLIM LSM 880PicoQuant

2016. F. Li et al. Reciprocal regulation of actin cytoskele-ton remodeling and cell migration by calcium and zinc: role of TRPM2 channels. doi:10.1242/jcs.179796

Journal of Cell Science HeLa cells PC-3 cells

Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. A. Petrosyan et al. The role of Rab6a and phosphorylation of non-muscle myosin IIA tailpiece in alcohol-induced Golgi disorganization. doi:10.1038/srep31962

NatureScientific Reports

HepG2 cells mouse ADH1 (VA-13 cells)

Fluorescent Immuno- histochemical staining

LSM 800 Airyscan

2016. P. Sheehan et al. Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway. doi:10.1523/JNEUROSCI.0725-16.2016

The Journal of Neuroscience

RatHippocampal neurons

Fluorescent Immuno- histochemical staining

LSM 800Airyscan

2016. R. L. Weaver et al. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. doi:10.7554/eLife.16620

eLIFE Mouse embryonic fibroblasts (MEF)

Fluorescent Immuno- histochemical staining

LSM 880

2016. B. Yao et al. Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells. doi:10.1016/j.cellsig.2016.08.003

Cellular Signalling Liver cancer cells LSM 800

2016. M. Zhou et al. VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking. doi:10.1083/jcb.201604061

JBCThe Journal of Cell Biology

U2OS cells Live cell imaging LSM 800Airyscan

2016. E. Adu-Gyamfi et al. Flexibility of the head-stalk linker domain of paramyxovirus HN glycoprotein is essential for triggering virus fusion. doi:10.1128/JVI.01187-16

Journal of Virology Paramyxoviridae LSM 800Airyscan

2016. L.Li et al. Real-time imaging of Hunting-tin aggregates diverting target search and gene transcription. doi:10.7554/eLife.17056

eLIFE Mouse D3 (ATCC, Manassas, VA) ES cells

Fluorescent proteinsAiryscanSIMSingle molecule localizationFRAP

LSM 880Airyscan

2016. J. Bruno et al. SEC16A is a RAB10 effector required for insulin-stimulated GLUT4 trafficking in adipocytes. doi:10.1083/jcb.201509052

JBCThe Journal of Cell Biology

Adipocytes Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. X. Xie et al. Rational Design of an α-Ketoamide-Based Near-Infrared Fluorescent Probe Specific for Hydrogen Peroxide in Living Systems. doi:10.1021/acs.analchem.6b01256

Analytical Chemistry Cell culture LSM 880

Application Note

8

Cell culture / In vitro / Fixed cells

Publication Journal Sample Application System

2016. D.C. Gershlick et al. TSSC1 is Novel Component of the Endosomal Retrieval Machinery. doi:10.1091/mbc.E16-04-0209

MBoCMolecular Biology of the Cell

Mouse tissue extractsWT, TSSC1-KO and VPS54-KO HAP1 cell lines

Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016, T. Yang et al. A Rapid and Convenient Method for in Vivo Fluorescent Imaging of Protoscolices of Echinococcus multilocularis. doi:10.3347/kjp.2016.54.2.225

The Korean Journal of Parasitology

Echinococcus multilocularis Live cell imaging LSM 800

2016, J. J. Kelly et al. Specific functional pathologies of Cx43 mutations associated withoculodentodigital dysplasia. doi:10.1091/mbc.E16-01-0062

MBoCMolecular Biology of the Cell

Primary human fibroblasts culturesHuman ODDD patient skin biopsies

Fluorescent Immuno- histochemical staining

LSM 800 Airyscan

2016, B. M. Laitman et al. The transcriptional activator Krüppel-like factor -6 is required for CNS myelination. doi:10.1371/journal.pbio.1002567

PLOS, Biology MouseOligodendrocyte progenitors cell culture

Fluorescent Immuno- histochemical staining

LSM 880

2016, F. M. Kuhlmann et al. Blood Group O–Dependent Cellular Responses to Cholera Toxin: Parallel Clinical and Epidemiological Links to Severe Cholera. doi:10.4269/ajtmh.16-0161

ASTHM, The American Journal of Tropical Medicine and Hygiene

Human enteroid cultureCholera toxin

Live cell imagingCRISPR

LSM 880

2016, M. Kargar et al. Colloidal Crystals Delay Formation of Early Stage Bacterial Biofilms. doi:10.1021/acsbiomaterials.6b00163

ACSBiomaterialsScience and Engineering

Pseudomonas aeruginosaBiofilm

LSM 880

2016, W. Quing Liu et al. A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA-MB-231. doi:10.3892/ol.2016.4538

Oncology LettersSpandidos Publications

MDA-MB-231 cells Live cell imagingTime series

LSM 800

2016, Y. Xing-Mei et al. Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6. doi:10.3892/mmr.2016.5167

Molecular Medicine ReportsSpandidos Publications

Cultured podocytes Ca-Imaging (Fluo-3) Live cell imagingTime series

LSM 880

2016, D. Kalafatovic et al. MMP-9 triggered self-assem-bly of doxorubicin nanofiber depots halts tumor growth. doi:10.1016/j.biomaterials.2016.04.039

Biomaterials MDA-MB-231-luc-D3H2LN cell culture

CRISPRFluorescent Immuno- histochemical staining

LSM 880 Airyscan

2016, H. Zuh et al. Parkinson’s disease-like forelimb akinesia induced by BmK I, a sodium channel modulator. doi:10.1016/j.bbr.2016.04.036

Behavioural Brain Research

Human neuroblastoma SHSY5Y

Fluorescent Immuno- histochemical staining

LSM 880

2016, M. O’Brien et al. CD4 Receptor is a key deter-minant of divergent HIV-1 sensing by plasmacytoid dendritic cells. doi:10.1371/journal.ppat.1005553

PLOS, Pathogenes HEK cellsPurified human pDC Incubated with GFP PR8 influenza 10 HAU, GFP Ha-HIV or GFP JRFL

Fluorescent Immuno- histochemical staining

LSM 880

2016, Y. Qiao et al. Melanoma cell adhesion molecu-le stimulates yes-associated protein transcription by enhancing CREB activity via c-Jun/c-Fos in hepatocellular carcinoma cells. doi:10.3892/ol.2016.4442

Oncology LettersSpandidos Publications

Human HCC Bel-7402, SMMC-7721 and Huh7 cells lines

Fluorescent Immuno- histochemical staining

LSM 800

2016, M. A. Rider et al. ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles. doi:10.1038/srep23978

NatureScientifc Reports

HEK293 cells Fluorescent Immuno- histochemical staining

LSM 880

2016, L. Xiaojin et al. Long-term performance and micro-bial community characterization of an osmotic anam-mox system for removing reverse-fluxed ammonium. doi:10.1016/j.biortech.2016.03.137

Bioresource Technology Biofilm FISH LSM 880

2016, J. HoJun et al. Nanostructured surface of electro-spun PCL/dECM fibres treated with oxygen plasma for tissue engineering. doi:10.1039/C6RA03840A

RSC Advances Cell culture in PCL/d-ECM scaffold

Fluorescent Immuno- histochemical stainingTissue formation and regeneration

LSM 800

Application Note

9

Cell culture / In vitro / Fixed cells

Publication Journal Sample Application System

2016, L. Hyeongjin. A scaffold with a bio-mimetically designed micro/nano-fibrous structure using decellula-rized extracellular matrix. doi:10.1039/C5RA27845G

RSC Advances Cell culture in PCL/d-ECM scaffold

Fluorescent Immuno- histochemical stainingTissue formation and regeneration

LSM 800

2016, R. Guo et al. Porcine reproductive and respiratory syndrome virus utilizes nanotubes for intercellular spread. doi:10.1128/JVI.00036-16

Journal of Virology porcine reproductive and respiratory syndrome virus (PRRSV) in HEK-293T cells

Live cell imaging LSM 880

2016, T. Bacon et al. Histone deacetylase 3 indirectly modulates tubulin acetylation. doi:10.1042/BJ20150660

Biochemical Journal Immortalized human prostate cancer cells (PC3)Human HDAC3 clones

Fluorescent Immuno- histochemical staining

LSM 880 Airyscan

2016, S. Skariah et al. The FIKK kinase of Toxoplasma gondii is not essential for the parasite’s lytic cycle. doi:10.1016/j.ijpara.2016.01.001

International Journal for Parasitology

Toxoplasma gondii in HFF cells

LSM 880 or 800Airyscan

2016, X. Li et al. Nitrogen removal by granular nitritation–anammox in an upflow membrane-aerated biofilm reactor. doi:10.1016/j.watres.2016.02.031

Water Research microorganisms (e.g. AOB, NOB and anam-mox bacteria) in the granule and biofilm

Microbial community analysis

LSM 880

2016, G.W. Dougherty et al. DNAH11 Localization in the Proximal Region of Respiratory Cilia Defines Distinct Outer Dynein Arm Complexes. doi:10.1165/rcmb.2015-0353OC

American Journal of Respi-ratory Cell and Molecular Biology

HumanTrachearespiratory epithelium

Fluorescent Immuno- histochemical staining

LSM 880Apotome

2015, Kitamura et al. Interaction of RNA with a C-terminal fragment of the amyotrophic lateral sclerosis-associated TDP43 reduces cytotoxicity. doi:10.1038/srep19230

NatureScientific Report

Mouse neuroblastoma Neuro2A cells transiently expressing GFP, TDP43-GFP, GFP-TDP35, GFP-TDP25, or GFP-NLS-TDP25

Fluorescent Immuno- histochemical staining

LSM 800Airyscan

2016, A. Jassim et al. Engineered Cx40 variants increased docking and function of heterotypic Cx40/Cx43 gap junction channels.doi:10.1016/j.yjmcc.2015.11.026

Journal of Molecular and Cellular Cardiology

Mouse neuroblastoma (N2A)human cervical carcinoma (HeLa) cells

Localizations of Cx40-YFP, mutant-YFP, Cx40-RFP and Cx43-RFP were studied by expressing these constructs individually in gap junction deficient HeLa and N2A cells.

LSM 800Airyscan

2015, Y. Suzuki-Karasaki et al. Distinct effects of TRAIL on the mitochondrial network in human cancer cells and normal cells: role of plasma membrane depolarization. doi:10.18632/oncotarget.4268

Oncotarget A549 cells (human lung adenocarcinoma epithelial cell line)Osteosarcoma cell linesHuman dermal fibroblasts from facial dermis

Mitochondrial network imaging and length measu-rements

LSM 880

2015, W. Ji et al. Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondri-al fission sites. doi:10.7554/eLife.11553

eLIFE Human osteosarcoma U2OS Live cell imagingDrp 1 maturation

LSM 880Airyscan

2015, K. A. Makowska et al. Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells. doi:10.1016/j.celrep.2015.11.012

Cell Reports LNCaP, DU145, and PC-3 cells1535NP and CT cells

Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016, S. Kang et al. Development of anti-biofouling in-terface on hydroxyapatite surface by coating zwitterionic MPC polymer containing calcium-binding moieties to prevent oral bacterial adhesion. doi:10.1016/j.actbio.2016.03.006

Acta Biomaterialia Bacterial suspension Streptococcus mutans

Fluorescent Immuno- histochemical stainingAnalysis of anti-biofouling surfaces

LSM 880

Application Note

10

Whole organisms / In vivo imaging

Publication Journal Sample Application System

2017. Zuber et al. The ABC transporter Snu and the extracellular protein Snsl cooperate in the formation of the lipid-based inward and outward barrier in the skin of Drosophila.doi:10.1016/j.ejcb.2017.12.003

European Journal of Cell Biology

Drosophila larvae In vivo imaging LSM 880AiryscanLSM 780LSM 710

2017. Liu et al. Symplastic communication spatially directs local auxin biosynthesis to maintain root stem cell niche in Arabidopsis.doi:10.1073/pnas.1616387114

PNAS Arabidopsis thaliana Live cell imaging LSM 880Airyscan

2017. Mohamed et al. Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins.doi:10.1186/s12915-017-0430-2

BMC Biology Zebrafish embryo In vivo imaging LSM 800

2017. Yi et al. Comparative Transcriptomics of Bacillus mycoides Strains in Response to Potato-Root Exudates Reveals Different Genetic Adaptation of Endophytic and Soil Isolates.doi:10.3389/fmicb.2017.01487

Frontiers in Microbiology Arabidopsis thalianaBacillus mycoides

Live cell imaging LSM 800

2017. Shi et al. Model for the role of auxin polar transport in patterning of the leaf adaxial-abaxial axis.doi:10.1111/tpj.13670

The Plant Journal Arabidopsis thaliana Live cell imaging LSM 880Airyscan

2016. K. Rao et al. Spastin, atlastin and ER relocalization are involved in axon, but not dendrite, regeneration. doi:10.1091/mbc.E16-05-0287

MBoCMolecular biology of the Cell

Drosophila In vivo imaging LSM 880Airyscan

2016. Besnard et al. UMAMIT14 is an amino acid exporter involved in phloem unloading in Arabidopsis roots.doi:10.1093/jxb/erw412

Journal of Experimental Botany

Arabidopsis thaliana Arabidopsis transient expression

LSM 880

2016. Iizuka et al. The role of Tks adaptor proteins in invadopodia formation growth and metastasis of melanoma. doi:10.18632/oncotarget.12954

Oncotarget C8161.9 cellspHluorinLifeact-mCherry

Live cell imaging LSM 880Airyscan

2016. Vonhoff and Keshishian. Cyclic nucleotide signaling is required during synaptic refinement at the Drosophila neuromuscular junction. doi:10.1002/dneu.22407 0.1002/dneu.22407

Developmental Neuro-biology

Drosophila melanogaster embryos

In vivo imagingGCaMp5

LSM 880Airyscan

2016. A. Koch et al. An RNAi-Based Control of Fusariumgraminearum Infections Through Spraying of Long dsRNAs Involves a Plant Passage and is Controlled by the Fungal Silencing Machinery. doi:10.1371/journal.ppat.1005901

PLOS, Pathogens Hordeum vulgare (Barley)Fusarium graminearum strain Fg-IFA65

In vivo imagingSpectral/ Lambda imaging (Linear unmixing, Online fingerprinting)

LSM 880

2016. E. Breeze et al. A C-terminal amphipathic helix is necessary for the in vivo tubule-shaping function of a plant reticulon. doi:10.1073/pnas.1605434113

PNAS Arabidopsis thaliana In vivo: ER network analysisFRAP

LSM 880Airyscan

2016. O. Sztatelman et al. Fine tuning chloroplast move-ments through physical interactions between phototropins. doi:10.1093/jxb/erw265

Journal of Experimental Botany

Arabidopsis thaliana Live imagingChloroplast movement

LSM 880

2016. M. R. Greaney et al. Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio. doi:10.1002/cne.24042

Journal of Comparative Neurology

Zebrafish Live cell imagingIn vivo imaging, mCherry

LSM 800

2016, H. L. Johnsen and H. R. Horvitz. Both the apoptotic suicide pathway and phagocytosis are required for a programmed cell death in Caenorhabditis elegans. doi:10.1186/s12915-016-0262-5

BMC Biology Caenorhabditis elegans Live cell imaging LSM 800

2016, J. Firmino et al. Cell Division Drives Epithelial Cell Rearrangements during Gastrulation in Chick. doi:10.1016/j.devcel.2016.01.007

Developmental Cell Chicken embryo Live imaging of gastrulation LSM 700LSM 880LSM 7 MP

Application Note

11

Organ, tissue, whole organisms

Publication Journal Sample Application System

2017. Nagaki et al. ePro-ClearSee: a simple immunohisto-chemical method that does not require sectioning of plant samplesdoi:10.1038/srep42203

nature Various Plants ClearingFluorescent Immuno- histochemical staining

LSM 800AiryscanLSM 700

2017. Edmond et al. Topoisomerase IIβ Selectively Regulates Motor Neuron Identity and Peripheral Connectivity Through Hox/Pbx-dependent Transcriptional Programs.doi:10.1523/ENEURO.0404-17.2017

eNeuro Mouse In Situ HybridizationWhole mount GFP labellingFluorescent Immuno- histochemical staining

LSM 800Apotome

2017. Johnston et al. Optogenetic targeting of cardiac myo-cytes and non-myocytes: Tools, challenges and utility.doi:10.1016/j.pbiomolbio.2017.09.014

Progress in Biophysics and Molecular Biology

Mousecardiac non-myocytes,hearts

Clarity - XFluorescent Immuno- histochemical staining

LSM 880Airyscan FastLSM 880 NLO

2017. Shavkuta et al. Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. doi:10.1088/1612-202X/aa963b

IOP ScienceLaser Physics Letters

Human cornea Second Harmonics Generation (SHG)

LSM 880 NLO

2017. Eekhoff et al. Functionally Distinct Tendons From Elastin Haploinsufficient Mice Exhibit Mild Stiffening and Tendon-Specific Structural Alteration.doi:10.1115/1.4037932

ASME Mouse Fluorescent Immuno- histochemical staining

LSM 880 NLO

2017. Kadala et al. Fluorescent labeling and 2-Photon imaging of Mouse tooth pulp nociceptors. doi:10.1177/0022034517740577

Journal of Dental Research MouseTrigeminal ganglion

Fluorescent Immuno- histochemical staining

LSM 880 NLO

2017. Plummer et al. Two Subpopulations of Noradrenergic Neurons in the Locus Coeruleus Complex Distinguished by Expression of the Dorsal Neural Tube Marker Pax7.doi:10.3389/fnana.2017.00060

Frontiers in Neuroanatomy MouseCoronal brain slices

PACT ClearingFluorescent Immuno- histochemical staining

LSM 880LSM 780

2017. Bohuslavova et al. Renal injury is accelerated by global hypoxia-inducible factor 1 alpha deficiency in a mouse model of STZ-induced diabetes.doi: 10.1186/s12902-017-0200-8

BMC MouseKidney sections

Fluorescent Immuno- histochemical staining

LSM 880 NLO

2017. Yuanwei et al. Derivation of cortical spheroids from human induced pluripotent stem cells in a suspension bioreactor. doi:10.1089/ten.tea.2016.0400

Tissue Engineering Part A Cortical spheroids Fluorescent Immuno- histochemical staining

LSM 880

2016. Yasui et al. Sensitization with vaccinia virus encoding H5N1 hemagglutinin restores immune potential against H5N1 influenza virus. doi:10.1038/srep37915

Nature Mouse Spleen sections

Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. Pesch et al. Drosophila chitinase 2 is expressed in chitin producing organs for cuticle formation. doi:10.1016/j.asd.2016.11.002

Arthropod Structure & Development

Drosophila melanogaster embryo

Fluorescent Immuno- histochemical staining

LSM 780Airyscan

2016. Ozdemir et al. Tuning Hydrogel properties to promote the assembly of salivary gland spheroids in 3D

ACS BiomaterialsScience Engineering

Spheroid Fluorescent Immuno- histochemical staining

LSM 880 NLO

2016. J.T. Ehmsen et al. The astrocytic transporter SLC7A10 (Asc-1) mediates glycinergic inhibition of spinal cord motor neurons. doi:10.1038/srep35592

NatureScientific Reports

MouseSlc7a10tm1Dgen

GLYT2-eGFPBAC GLT1-eGFP

Fluorescent Immuno- histochemical staining

LSM 800LSM 880Airyscan

2016. Y. Yoshimura et al. Roles of 5-HT1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons. doi:10.1016/j.neures.2016.09.008

Neuroscience Research Mouse Fluorescent Immuno- histochemical staining

LSM 800Airyscan

2016. F. Mora-Bermúdez et al. Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development. doi:10.7554/eLife.18683

eLIFE Cerebral organoids Fluorescent Immuno- histochemical staining

LSM 880 Airyscan

Application Note

12

Organ, tissue, whole organisms

Publication Journal Sample Application System

2016. Y. Shinohara et al. Viral Vector-Based Dissection of Marmoset GFAP Promoter in Mouse and Marmoset Brains. doi.org/10.1371/journal.pone.0162023

PLOS, ONE MiceMarmosetBrain tissue sections

Fluorescent Immuno- histochemical staining

LSM 880

2016. T.-M. Fu et al. Stable long-term chronic brain mapping at the single-neuron level. doi:10.1038/nmeth.3969

Nature methods Mouse Fluorescent Immuno- histochemical staining

LSM 880

2016. M. J. Costello et al. Identification and Ultrastructural Characterization of a Novel Nuclear Degradation Complex in Differentiating Lens Fiber Cells. doi:10.1371/journal.pone.0160785

PLOS, ONE Chicken embryosEye lenses

Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016. S.J. Miller and J.D. Rothstein. Astroglia in thick tissue with super resolution and cellular reconstruction. doi:10.1371/journal.pone.0160391

PLOS, ONE Mouse, BAC-GLT1-eGFP and 8.3kb-tdTomato

Clearing, passive ClarityFluorescent Immuno- histochemical staining

LSM 800 Airyscan

2016. T.G. Mc Williams et al. mito-QC illuminates mitophagy and mitochondrial architecture in vivo. doi:10.1083/jcb.201603039

JBCThe Journal of Cell Biology

Mouse (Brain, Kidney)mito-QC MEFs

Fluorescent Immuno- histochemical stainingFluorescent proteinsLive cell imagingClearing

LSM 880Airyscan

2016. V. Zazueta-Novoa et al. Complexity of Yolk Proteins and Their Dynamics in the Sea Star Patiria miniata. Biol. Bull. June 1, 2016 vol. 230 no. 3 209-219

The Biological Bulletin Patiria miniata (Sea Star)Tissue sections

Fluorescent Immuno- histochemical staining

LSM 800

2016. C. Dhang et al. Bio-Inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering. doi:10.1016/j.biomaterials.2016.07.007

Biomaterials Collagen scaffold for bone tissue

LSM 800Airyscan

2016. H. Ren et al. SPIKE1 activates ROP GTPase to modulate petal anisotropic growth in Arabidopsis. doi:10.1104/pp.16.00788

Plant Physiology Arabidopsis thaliana Fluorescent Immuno- histochemical staining

LSM 880

2016, A. Sherlekar and R. Rikhy. Syndapin promotes pseu-docleavage furrow formation by actin organization in the syncytial Drosophila embryo. doi:10.1091/mbc.E15-09-0656

MBoCMolecular Biology of the Cell

Drosophila melanogster Fluorescent Immuno- histochemical staining

LSM 800 Airyscan

2016, K. N. Richter et al. Secretory cells in honeybee hypo-pharyngeal gland: polarized organization and age-dependent dynamics of plasma membrane.doi:10.1007/s00441-016-2423-9

Cell and tissue Research Apis mellifera (honeybee)Hypopharyngeal gland

Fluorescent Immuno- histochemical staining

LSM 880 Airyscan

2016, S. J. Terrill et al. Role of Lateral Septum Glucagon-Like Peptide 1 Receptors in Food Intake. doi:10.1152/ajpregu.00460.2015

American Physiological Society

Rat Fluorescent Immuno- histochemical staining

LSM 880

2016, S.-N. Wang et al. Cloning and expression profile of io-notropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae). doi:10.1016/j.jinsphys.2016.05.002

Journal of Insect Physiology Microplitis mediator (Hy-menoptera, Braconidae)Antennae

Fluorescent Immuno- histochemical staining

LSM 880

2016, H. Li et al. ROCK inhibitor abolishes the antibody response in experimental autoimmune myasthenia gravis.doi:10.1016/j.mcn.2016.05.001

Molecular and Cellular Neuroscience

RatGastrocnemius muscle tissue

Fluorescent Immuno- histochemical staining

LSM 800

2016, P. Sandeep et al. Ihha induces hybrid cartilage-bone cells during zebrafish jawbone regeneration. doi:10.1242/dev.131292

Development Zebrafish Fluorescent Immuno- histochemical stainingFluorescent in situ Hybri-disation

LSM 800

2016, S. Lei et al. Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency. doi:10.1038/srep25222

NatureScientific Reports

Intestinal tissue sections from RAG2/IL2RG deficient pigs(CRISPR/Cas9)

Fluorescent Immuno- histochemical staining

LSM 880

Application Note

13

Organ, tissue, whole organisms

Imaging Methods

Publication Journal Sample Application System

2016, K. Kolahi et al. Real-time tracking of Bodipy-C12 long-chain fatty acid in human term placenta reveals unique lipid dynamics in cytotrophoblast cells. doi:10.1371/journal.pone.0153522

PLOS, ONE Human placenta explants Fluorescent Immuno- histochemical staining

LSM 880Airyscan

2016, S. Lei et al. Enterobacter cloacae inhibits human norovirus infectivity in gnotobiotic pigs. doi:10.1038/srep25017

NatureScientific Reports

Pig, small intestinal tissue Fluorescent Immuno- histochemical staining

LSM 880

2016, Y. Leonhardt et al. Functional characterization of the Woronin body protein WscA of the pathogenic mold Aspergil-lus fumigatus. doi:10.1016/j.ijmm.2016.03.008

Internal Journal of Medical microbiology

Aspergillus fumigatus Fluorescent Immuno- histochemical staining

LSM 880 Airyscan

2016, K. A. Halberg et al. The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules. doi:10.1038/ncomms11266

NatureCommunications

Drosophila melanogaster Fluorescent Immuno- histochemical staining

LSM 880Airyscan

K. Meng-Tsen et al. Super-Resolution Mapping of Neu-ronal Circuitry With an Index-Optimized Clearing Agent. doi:10.1016/j.celrep.2016.02.057

Cell Reports Mouse (brain, oocyte)Drosophila melanogasterHEK293T cells

Water based clearing, SeeDBFluorescent proteinsFluorescent Immuno- histochemical staining

LSM 880 Airyscan

2016, I. M. Berke et al. Seeing through Musculoskeletal Tissues: Improving In Situ Imaging of Bone and the Lacunar Canalicular System through Optical Clearing. doi:10.1371/journal.pone.0150268

PLOS, ONE MouseSkeletal muscle tissue

Clearing (water-based)Comparison of clearing methodsFluorescent Immuno- histochemical staining

LSM 880LSM 780 NLO

2015, P.P. de Abreu Manso et al. Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. doi:10.1371/journal.pntd.0004064

PLOS, Neglected Tropical Diseases

Immunology Chicken embryos

Histological sectionsFluorescent Immuno- histochemical stainingAssay

LSM 710LSM 880AiryscanElyra SR-SIM

Publication Journal Sample Application System

2017. Korobchevskaya et al. Exploring the Potential of Airyscan Microscopy for Live Cell Imaging.doi:10.3390/photonics4030041

Photonics HeLa cellsRat Basophilic Leukaemia cell (RBL)

Live cell imagingFluorescent Immuno- histochemical staining

LSM 880Airyscan

2017. Kolossov et al. Airyscan super-resolution microsco-py of mitochondrial morphology and dynamics in living tumor cells.doi:10.1002/jemt.22968

Microscopy Research & Technique

U87 glioblastoma cellsA549 lung cancer cells

Live cell imaging LSM 880Airyscan Fast

2017. Zamir et al. Reconceptualizing Fluorescence Correlation Spectroscopy for Monitoring and Analyzing Periodically Passing Objects.doi:10.1021/acs.analchem.7b03108

ACS Publications CHO suspension cells in droplets

FCS LSM 880

2017. Perry et al. Production of 3D Printed Scale Models from Microscope Volume Datasets for use in STEM Educa-tion. EMS Eng Sci j 2017, 1(1):002

EMS Engineering Science Journal

Various 3D Printing LSM 880Lightsheet Z.1

2016. Comparison of multi-mode parallel detection microscopy methods

Optics communications LSM 800Airyscan

2016. Li et al. Method of super-resolution based on array detection and maximum-likelihood estimation. doi:10.1364/AO.55.009925

Applied Optics Fixed cells Comparison conventional confocal and array detection using pixel reassignment

Airyscan

Application Note

14

References

[1] Conchello, J.-A. and Lichtman, J.W., Optical sectioning microscopy. Nature methods, 2005. 2(12): p. 920 – 931.

[2] Minsky, M., Memoir on inventing the confocal scanning microscope. Scanning, 1988. 10(4): p. 128 – 138.

[3] Huff, J., The Airyscan detector from ZEISS: confocal imaging with improved signal-to-noise ratio and super-resolution.

Nature methods, 2015. 12.

[4] Weisshart, K., The basic principle of Airyscanning. 2014. ZEISS Technology Note

[5] Huff, J.; Bathe, W.; Netz, R.; Anhut, T.; Weisshart, K., The Airyscan detector from ZEISS. Confocal imaging with improved signal-to-noise

ratio and superresolution. 2015. ZEISS Technology Note

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Imaging Methods

Publication Journal Sample Application System

2016. J. Kang et al. A solvent depend on ratiometric flu-orescent probe for hypochlorous acid and its application in living cells. doi:10.1016/j.dyepig.2016.09.048

Dyes and Pigments A549 cell culture Fluorescent sensorLive cell imaging

LSM 880Airyscan

2016. J. Miao et al. A new class of fast-response and highly selective fluorescent probes for visualizing peroxyni-trite in live cells, subcellular organelles, and kidney tissue of diabetic rats. doi:10.1016/j.biomaterials.2016.08.032

Biomaterials RatLive cellsTissue

LSM 880Airyscan

2016. G. Follain et al. Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology. doi:10.1242/jcs.189001

Journal of Cell Science C. elegansZebrafishMouse

Comparisons of systems for in vivo imaging

Airyscan

2016. M. Sivaguru et al. Comparative performance of airyscan and structured illumination superresolution mi-croscopy in the study of the surface texture and 3D shape of pollen. DOI: 10.1002/jemt.22732

Microscopy Research and Technique

Pollen Unlabeled and labelled pollenComparison of Confocal, Airyscan, and SR-SIM

LSM 880Airyscan

2016, Y. Zhao et al. A new far-red naphthorhodamine dye: Synthesis, fluorescent probe and bioimaging appli-cations. doi:10.1016/j.dyepig.2016.05.012

Dyes and Pigments HeLa cell culture Far-red naphthorhodamine dye Labelling mitochondriaLive cell imaging

LSM 880

2016, M. A. Urban eta al. Cuticle and subsurface orna-mentation of intact plant leaf epidermis under confocal and superresolution microscopy. doi:10.1002/jemt.22667

Microscopy Research and Technique

Ginko biloba, intact plant leafs

Clearing, embedding media analyzed for confocal and superresolution microscopy

LSM 880 Airyscan

2016, J. Chao et al. A single fluorescent probe for multiple analyte sensing: efficient and selective detection of CN−, HSO3− and extremely alkaline pH. doi:10.1039/C6TB00119J

Materials for biology and medicine

E. coli cells IECBTDetection of analytes inclu-ding CN-, HSO3-, alkaline pH

LSM 880

Carl Zeiss Microscopy GmbH 07745 Jena, Germany microscopy@zeiss.com www.zeiss.com/lsm880

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Copyright Notice (Cover image):

Upper left: Sample: courtesy of P. Janz, Neuropathology, University Freiburg, Germany

Upper right: Images courtesy: Tong Xiao and Beth Carroll. Isacoff Labs. University of California. Berkeley.

Bottom left: COPYRIGHT: Yi Zuo, UCSC

Bottom right: Courtesy of: Prof. Chris Hawes, Dept. Biological & Medical Sciences, Oxford Brookes University