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What is a zebra fish? Tropical freshwater fish Minnow family Generation time is ~3-4 months Adults ~ 4cm in length Omnivorous Eats primarily zooplankton, phytoplankton, insects and larvae
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What is a zebra fish? Tropical freshwater fish Minnow family Generation time is ~3-4 months Adults ~ 4cm in length Omnivorous
Eats primarily zooplankton, phytoplankton, insects and larvae
Why are zebra fish important? The embryos are see-through
allows us to watch the changes in development Adults breed rapidly
good supply of embryos for our research Embryos develop quickly
4-5 days old the embryos become swimming larvae and start to catch their own food
Zebra Fish Development Close homology to humans
More closely related to humans then models such as the worm and fruit fly, easier to use than mouse/rat
Development
UNFERTILIZED
FERTILIZED
0 HPF 1 HPF 2 HPF 4 HPF 8 HPF 12 HPF 16 HPF 24 HPF 36 HPF 48 HPF 72 HPF
HPF=Hours Post Fertilization
Zebra in the classroom
Read through the zebra in the classroom article with a partner
What must be done in order for zebra fish to successfully breed?
Why are zebrafish embryos helpful for studying development?
What does hpf stand for? What is it about zebra fish physiology that makes
them good models to study in the lab? Explain why biochemistry and genetics are important
aspects to understand about the zebra fish. Is it right to say that zebra fish are a “research tool”? What information is most important to know for
conducting an experiment with zebra fish?
Salt Solution (Test Salinity) Concentration
mg/Liter
Stock (40.0g/Liter) I.O.
SolutionDistilled Water
60 .75 mL 499.25 mL
120 1.5 mL 498.5 mL
240 3.0 mL 497.0 mL
480 6.0 mL 494.0 mL
1000 12.5 mL 487.5 mL
10,000 125 mL 375.0 mL
Must use 3 Concentrations of your choice
pH Solution (Test acidity)
Must Use 3 Concentrations of your choice ADD ACID TO WATER NEVER WATER TO ACID!!!
Solution
Final volume
of 500 mL
40 g/L Stock
Solution I.O
Solution
Distilled Water
Part 1
Additions to
adjust pH to
final pH
pH 4.0 0.25 mL 150 mLAdd 0.1M HCl
until pH 4.0
pH 7.0 0.25 mL 150 mL
Add either 0.1M
HCl to lower or
1M NaHCO3to
raise to a final
pH 7.0
pH 10.0 0.25 mL 150 mL
Add 0.1M
NaOH until pH
10
Ethanol[Final] in mM [Stock] in
mM
mL of Stock mL of Embryo
Media
Total volume
in mL
300 1000 100 350 450
100 1000 33 300 333
30 300 33 300 333
Unstable…must make the day before useMust use 3 Concentrations of your choice
Zebrafish Development - Zygote “The Bubble” Zygote Period – 0 – 0.75 hpf
Fertilized Egg to develop with its first division of cells
Cleavage Period “The bubbles boil over”
Cleavage - 0.75 – 2.25 hpf
Cells begin to divide… still large enough to count
Blastula Period “The afro” Blastula- 2.25-5.25 hpf
Cells multiply very rapidly at the top
Gastrula Period – “The Solar Eclipse”
Gastrula - 5.25- 10.33 hpf
Outside of the cell begins to develop
Segmentation Period “ The six pack abs”
Segmentation - 10.33 – 24.0 hpf
Somites form around the midsection for segmentation.
Pharyngula Period “The fat belly” Pharyngula - 24.0-48.0 hpf
Fish has large yolk sack present/ Body straightens
Hatching Period “The free fish” Hatching - 48.0-72.0 hpf
