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You are… just a bunch of CELLS
LECTURE 04 – Cell Theory, Structure, Etc. CH 4
Cell Theory & Inside the CellSpring 2013 - Althoff Reference: Mader & Windelspecht Ch. 4)
Lec04
• ___________ – unifying concept in Biology
• First proposed by biologists _________ and __________ in 1838-1839.
______ to the theory: 1) ______________ are composed of
cells 2) All cells come from ____________ cells 3) The cell is the ____________ of life
Cell Size• All size much ____________________ across• Some cells as small as 1 micrometer (mm)• Size restricted by ______________________________
1) Surface is membrane, across which cells takes in nutrients and exports waste
2) Volume is living cytoplasm, which demands nutrients and produces wastes 3) As cell grows, volume increases faster than surface
4) Cells specialized in absorption modified togreatly increase surface area per unit
volume
Surface-to-Volume Ratio
Total Surface Area 96 cm2 192 cm2 384 cm2
Total Volume 64 cm3 64 cm3 64 cm3
S/V Ratio 1.5 : 1 3 : 1 6 : 1
________________ Cells: CHARACTERISTICS
• Lack a membrane bound nucleus
• Structurally simple:Domain Bacteria 3 shapes: bacillus (rod)
coccus (spherical)spirilla (spiral)
Domain Archaea 3 shapes above pluslobed, plate-like, and irregularshaped (live in extreme environments)
Prokaryotic Cells: CHARACTERISTICS
(Mader p66Fig. 4.4)
CONJUGATION PILUS
FIMBRIAE
FLAGELLUM
INCLUSION BODYMESOSOME
RIBOSOME
NUCLEOID
PLASMAMEMBRANE
CELL WALL
GLYCOCALYX
• GLYCOCALYX – gel-like coating outside cell wall. If compact = ________. If diffuse = ________. Layer of _______________. Outside cell wall
• CELL WALL - _____________________
• PLASMA MEMBRANE -__________ entrance and exit of ______________
Prokaryotic Cells: CELL ENVELOPE
• CYTOPLASMA– _________________. Contains inclusion bodies (stored granules of various substance)
• FLAGELLUM(A) – ____________________ present in some bacteria, pushes cell forward
• FIMBRIAE – hair-like bristles that allow _________ to surfaces
• CONJUGATION PILI(US) – hollow appendages used for _________________ bacterial cells
Prokaryotic Cells: CYTOPLASMA & APPENDAGES
APPENDAGES
• RIBOSOMES – protein ___________ • MESOSOMEs– portion of plasma membrane that
folds into the cytoplasma… __________________• INCLUSION BODY – ____________ compartments
for nutrients• NUCLEOID – houses bacterial ______________• THYLAKOIDS – found in cyanobacteria (bacteria that
can photosynthesize). Internal membranes that ____________________
Prokaryotic Cells: other “parts”
______________ Cells: CHARACTERISTICS
• “3rd domain”. Kingdoms: Protista, Fungi, Plantae, Animalia
• _____________________ nucleus
• Highly ______________ organelles• Plasma __________________________________ into
and out of cytoplasma
• Plantae (i.e., plants)– have cell walls (different composition than bacteria cell walls)
Characteristics of Cells—in general
• In ____________ organisms, can—for a short period of time—remove cells from the organisms, provide the cells with food, and they can live independently for a short period of time
• They ________ their food and __________• They make __________________ ATP• They get ____ of their own _________• Have “______________” required for life.
Eukaryotic Cells: ORGANELLES
• Eukaryotic cells _______ than prokaryotic cells…result is different S/V ratio. Overcome this by having ________that have ___________ and perform specific functions
• Compartmentalization allows for isolated “___________”
Organelles that ________________ with each other through membrane channels or vesicles
___________ organelles: _______________________
TWO CLASSES OF ORGANELLES
CYTOSKELETON
Eukarya: ANIMAL CELLPLASMA
MEMBRANE
LYSOSOME
MITOCHONDRION
ENDOPLASMICRETICULUM
Rough ERSmooth ER
RIBOSOMES
NUCLEUS
VESICLE
GOLGI APPARATUS (Mader p68 Fig. 4.6)
CYTOPLASMA
RIBOSOMES
Eukarya PLANT CELL
NUCLEUS
ENDOPLASMICRETICULUM
Rough ERSmooth ER
CELL WALL
GOLGI APPARATUSCYTOPLASMA
PLASMAMEMBRANE
MITOCHONDRION
GRANUM stackedin CHLOROPLAST
CENTRAL VACUOLE
(Mader p69 Fig. 4.7)
CYTOPLASMA• Cell is ____ an empty sac• ________ inside cell• Composition tightly regulated. Consists of:
_______________________(think H+, Cl-, Na-, etc.)________ (including enzymes) etc.
• ___________ than composition of liquid outside cell• _________________ responsible for __________ cytosol
composition
ORGANELLES defined• NUCLEUS --________________–regulates all
functionsChromatin (contains ….._____________)Nucleolus produces _____________Ribosomes (some here)
• CYTOSKELETON _____________& assist with ____________ of cell parts
Microtubules __________ of protein moleculesIntermediate filaments ____________ Actin filaments ____________ of cell & organelles Centrioles there…but still not sure of function… not in plant cells
ORGANELLES defined• ENDOPLASMIC RETICULUM --________________,
_________________ with two major areas:Rough ER has ribosomes (_______ synthesis here)Smooth ER no ribosomes (_______ synthesis here)“the __________________” of the cell
(Mader p72 Fig. 4.10)
ORGANELLES defined• GOLGI APPARTUS – “the ____________________
department”• LYSOSOMES – “the _________________ center”
• MITOCHRONDRIA-- “the __________________”
• VESICLES—”_________________________”
• CENTRAL VACUOLE (plants)—”________________&________________ maintenance”
• PEROXISOMES (plants)– “enclose enzymes, in plants they __________________________that can be converted to sugars”
Original Prokaryotic cell
Cell gains an nucleus by the plasma membrane invaginating and surrounding the DNA with a
double membrane
Cell gains an endomembrane system byproliferation of membrane
Cell gains mitochondria(“captures” aerobic bacteria)
Cell gains chloroplasts(plant cells only)
Eukaryotic: Animal cell
Eukaryotic: Plant cell
2
3
4
1
Current hypothesis fororigin of organelles
How did we get from PRO- to EUKARYOTIC cells? DNA
ENERGY-RELATED Organelles
• Use ____________ to make carbohydrates – a chemical energy conversions
• _______________ have chloroplasts (but _________________ can also carry on photosynthesis)
• Contain _____________• Type of plastid—bounded by
double membrane• ThylakoidsGranum• Have their “______” DNA
• __________________ to produce _____ molecules—the energy currency for cells
• Up to a 1,000 in a single cell (ex. liver)
• Usually ______ within the cells ________ energy is burned
• Two key areas:cristae very ___________
(inner membrane), high S/V ratio
matrix: mitochrondrial _______________
CHLOROPLASTS MITOCHRONDRIA
CYTOSKELETON – a closer look• Lots of protein components—actually add
structure/organization to cytoplasm
• Contains actin filaments, intermediate filaments, and microtubules…that enables cell to retain shape AND move. Equivalent to human organism’s muscular and skeleton system at the cell level
Mader p78
CYTOSKELETON – Component functions• ________ FILAMENTS —movement of cell and
organelles• _______________ FILAMENTS—”rope-like” fibrous
polypeptides. Suspend/support various structures, organelles
• ____________—globular proteins called tubulin. Assist in the movement of vesicles, other organelles.
Mader p78-79
Centrioles
• ________ cylinders with 9 + 0 pattern of microtubules (vs. 9 + 2 pattern in cilia and flagella)
• Important during __________ when centroiles duplicate
Mader p80, Fig. 4.19
CILIA & FLAGELLA (FLAGELLUM sing.)
• __________ projections
• Move in whip-like or oar-like action ________ ___________________
• Paramecia –______Sperm--
________• Upper respiratory tract
— ___________
9 + 2 arrangement of microtubules
Mader p81, Fig. 4.20
Know Differences/Similarities of Prokaryotic vs. Eukaryotic Cells
(animal and plant)• Cell wall ?• Plasma membrane?• Nucleus or just nuclear
material?• Endoplasmic reticulum?• Golgi apparatus?• Lysosomes?• Mitochondria?• Chloroplasts?
• 9+ 2 cilia or flagella?• Centrioles ?• Which are smaller?
1 -20 mm vs.
10 -100 mm?
ConsultMader
p82, Table 4.1