Yemen Republic Ministry of Higher Education and

Scientific Research Al-Razi University

College of Medical Sciences

A Research Project Submitted to the Medical Laboratory Department, Faculty of Medical Sciences, Al-Razi University as a Partial Fulfillment For Bachelor Degree of Laboratory

Esmail Ahmed Obaid

Yousef Abdullah Fadhaeel

Osama Ahmed AL-Haidari

Ali Mohammed Ziad

Ziad Sadeq Al-Hawri

Maha Ahmed Al-JowmariRana

Manal Saad AL-Ward

Shrook Naji Rubaid

Supervisor Dr. Amal Mohammed Banafa

Assistant Professor of Clinical Biochemistry Al-Razi University

2019

By

Introduction

Gout is a systemic disease that results from the

deposition of monosodium urate crystals in tissues.

Increased serum uric acid above a specific threshold is a

requirement for the formation of uric acid crystals.

Despite the fact that hyperuricemia is the main

pathogenic defect in gout, many people with

hyperuricemia do not develop gout or even form UA

crystals. In fact, only 5% of people with hyperuricemia

above 9 mg/dL develop gout. Accordingly, it is thought

that other factors such as genetic predisposition share in

the incidence of gout.

Objective of the Study

General objective:

To evaluate prevalence of gout disease among Al-

Razi University student.

Specific objective:

To estimate the prevalence of gout and hyperuricemia

based on the representative sample of Al-Razi students.

To determine whether the prevalence of gout and/or

clinically significant hyperuricemia increased in a

managed care population.

Methodology

Study Design:-

A descriptive cross sectional study design was used in this

study.

Study Setting:-

The study was conducted in Al-Razi University in Sana’a-

Yemen.

Study Population:-

All the students study in Al-Razi University in

Sana’a- Yemen.

Sampling Method:-

Convenient sampling method was conducted in

this study.

Sample Size:

The final sample size was healthy Al -Razi

students aged 19-24 years.

Data Collection Technique

Approval was obtained from ethical committee of Al-Razi

University.

An official written permission to conduct the study was

obtained by the investigators from responsible authorities.

Verbal explanation of the nature and aim of the study was

performed to students.

Oral consent was taken from all students in the study

and they have the right refuse to participate or to

withdraw from the study without any effect on the

study.

The subjects who agree to participate in the study

were interviewed by the investigators to collect the

necessary data regarding gout after explaining the

purposes of the study.

Method of Data Collection

Blood samples were collected from each student.

The blood samples were centrifuged immediately and the

serum was separated.

The serum was divided into append of tube, those for uric

acid was stored at -20 °C and analyzed in the second day

of separation.

Enter the solution inside the rack located in the device; the

device recognizes the solution directly.

We enter the list of solutions in the computer of the

device, and then choose the name of the solution to be

filled, then we leave the rack of the device to add samples.

Add to each field in the rack, where we add 100 μm

solution from the patient's serum.

Enter the list of samples in the computer and start labeling

the samples inside the computer according to the sample

number added to the solution.

Then we select the name of the scan that we want to do;

then press the Save button and then determine the number

of the rack we added the samples and the location of the

sample within the rack and then press the OK button.

We go back to the device and then the device to identify

the sample and work directly.

Go back to the computer of the device; click on the list of

results and choose the sample number that we want to

know the result.

RESULTS

8.4%

21.3% 21.3%

17.8%

8.9%

12%

0

5

10

15

20

25

19year 20year 21year 22year 23year 24year

Figure (1): Distribution of student according to age.

Figure (2): Distribution of student according to sex

60% 40%

Females Male

Figure (3): Distribution the prevalence gout injury of

student.

13.3%

86.8%

Yes No

Table (1): The mean score of students according to sex

regarding gout injury.

Sex Gout injury

N Minimum Maximum Mean±SD

Females 134 0 10 5.28±1.28

Male 91 2 8 4.48±1.29

Demographical

data

Gout injury level

Yes No P-value

Pearson

Correlation F % F %

Age:

19years 10 4.44% 32 14.22%

0.019 .156*

20years 8 3.56% 40 17.78%

21years 5 2.22% 43 19.11

22years 6 2.67% 34 15.11

23years 1 0.44% 19 8.44

24years 0 0.00% 27 12

Sex:

Females 22 9.78% 112 49.78% 0.000 -.294-**

Male 8 3.56% 83 36.88%

Table (2): Correlation between student gout injury with age and

sex

Conclusion

It has been conclude that:-

The present study showed that, the most of student

ware female 60%, belonged to age 20 year old and 21year

old. The total prevalence of gout injury was 13.3% among

the student with age group (19 to 24 year). Their were

statistical significant different between gout prevalence with

age and sex (p 0.019, 0.000 respectively).

Recommendations

Increase in the number of samples, work on several

age groups, previous research studies to search for genetic

disorders, study of the relationship of uric acid and kidney

disease.