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Quantitative Analysis Sample Report
document.xlsx Page 1 of 48 Printed at: 05:52:07 on: 05/09/2023
Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal03.d Sample Name 25 StdSample Type Calibration Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 10:50Position P1-F3 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Units
7-amino clonazepam 0.982 2400 4223 0.5683 24.8930 ng/ml
1.174 1039 4223 0.2461 25.1833 ng/ml
zopiclone 2.465 4242 4223 1.0045 25.3477 ng/mlclonazepam D4-clonazepam 3.194 4519 8704 0.5192 24.8839 ng/mlflunitrazepam D4-clonazepam 3.286 5785 8704 0.6646 25.3495 ng/ml
alpha-OH alprazolam D4-clonazepam 3.518 2521 8704 0.2897 25.2873 ng/mltriazolam D5-temazepam 3.902 1100 12909 0.0852 24.6068 ng/mloxazepam D5-oxazepam 4.169 5393 10316 0.5228 25.4768 ng/mlflurazepam D5-oxazepam 4.035 3691 10316 0.3578 24.9591 ng/mlalprazolam D5-alprazolam 4.056 3517 6986 0.5034 25.1376 ng/mllorazepam D5-oxazepam 4.106 3595 10316 0.3485 25.0026 ng/ml
desalkylflurazepam D5-temazepam 4.545 2062 12909 0.1597 25.2525 ng/mltemazepam D5-temazepam 4.653 3570 12909 0.2765 25.1369 ng/mlchlordiazepoxide D5-nordiazepam 5.391 2372 4039 0.5871 27.2942 ng/mlnordiazepam D5-nordiazepam 5.816 3202 4039 0.7927 26.3845 ng/mlmidazolam D5-nordiazepam 6.334 2199 4039 0.5443 26.4673 ng/mldiazepam D5-nordiazepam 6.438 1605 4039 0.3975 26.3715 ng/ml
D4-7-amino clonazepam
7-amino flunitrazepam
D4-7-amino clonazepamD4-7-amino clonazepam
+ TIC MRM (** -> **) Benzo Cal03.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 3x10
0
0.5
1
1.5
2
2.5
3
3.5
![Page 2: [XLS] · Web viewISTD Response Schema Parameter Type Value Comment Include Header Boolean Include Footer Landscape Fit to Integer Page(s) wide Page(s) tall Hide ID Columns Use Options](https://reader042.dokumen.tips/reader042/viewer/2022022523/5b3284cd7f8b9aae458c0abf/html5/page/2.jpg)
Quantitative Analysis Sample Report
document.xlsx Page 2 of 48 Printed at: 05:52:07 on: 05/09/2023
Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal03.d
Acquisition Time (min)0.5 1 1.5
Coun
ts 2x10
01234567
7-amino clonazepam
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Ratio = 79.1 (95.8 %)
+ MRM (0.934-1.117 min, 43 scans) (286.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 3x10
012345678
121.0
222.0
286.0
+ MRM (284.0 -> 226.0) Benzo Cal03.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts 2x10
0
0.5
1
1.5
2
2.5
3
7-amino flunitrazepam
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Ratio = 68.5 (87.7 %)
+ MRM (1.092-1.252 min, 38 scans) (284.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 3x10
00.5
1
1.5
2
2.5
3
3.5
226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal03.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
1
1.2zopiclone
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Ratio = 57.8 (102.1 %)
+ MRM (2.395-2.592 min, 47 scans) (389.1 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)250 300 350
Coun
ts 4x10
00.20.40.60.8
11.21.41.61.8
245.1
217.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal03.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
clonazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Ratio = 24.2 (105.2 %)
+ MRM (3.105-3.313 min, 49 scans) (316.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
1.2
1.4270.0
214.0
316.0
![Page 3: [XLS] · Web viewISTD Response Schema Parameter Type Value Comment Include Header Boolean Include Footer Landscape Fit to Integer Page(s) wide Page(s) tall Hide ID Columns Use Options](https://reader042.dokumen.tips/reader042/viewer/2022022523/5b3284cd7f8b9aae458c0abf/html5/page/3.jpg)
Quantitative Analysis Sample Report
document.xlsx Page 3 of 48 Printed at: 05:52:08 on: 05/09/2023
flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal03.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
1
1.2
flunitrazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
314.0 -> 268.0, 314.0 -> 239.0Ratio = 53.3 (106.9 %)
+ MRM (3.188-3.498 min, 73 scans) (314.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 4x10
00.20.40.60.8
11.21.41.61.8
268.0
239.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal03.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 2x10
0
1
2
3
4
5
alpha-OH alprazolam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Ratio = 34.5 (103.1 %)
+ MRM (3.418-3.646 min, 54 scans) (325.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 3x10
012345678
297.0
216.0
325.0
+ MRM (343.0 -> 308.0) Benzo Cal03.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 2x10
00.250.5
0.751
1.251.5
1.752
triazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
1.2
343.0 -> 308.0, 343.0 -> 239.0Ratio = 100.0 (114.5 %)
+ MRM (3.828-4.067 min, 57 scans) (343.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts 3x10
0
0.5
1
1.5
2
2.5
3308.0239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal03.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
1
oxazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
287.0 -> 241.0, 287.0 -> 269.0Ratio = 29.2 (102.3 %)
+ MRM (4.077-4.299 min, 53 scans) (287.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)240 250 260 270 280
Coun
ts 4x10
0
0.2
0.4
0.60.8
1
1.2
1.4
241.0
269.0
287.0
![Page 4: [XLS] · Web viewISTD Response Schema Parameter Type Value Comment Include Header Boolean Include Footer Landscape Fit to Integer Page(s) wide Page(s) tall Hide ID Columns Use Options](https://reader042.dokumen.tips/reader042/viewer/2022022523/5b3284cd7f8b9aae458c0abf/html5/page/4.jpg)
Quantitative Analysis Sample Report
document.xlsx Page 4 of 48 Printed at: 05:52:08 on: 05/09/2023
flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal03.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 2x10
0
1
2
3
4
5
6
flurazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.40.6
0.81
1.2
388.0 -> 315.0, 388.0 -> 288.0Ratio = 14.5 (100.5 %)
+ MRM (3.935-4.203 min, 63 scans) (388.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)300 320 340 360 380
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1315.0
288.0
388.0
+ MRM (309.0 -> 281.0) Benzo Cal03.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 2x10
01234567
alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
309.0 -> 281.0, 309.0 -> 274.0Ratio = 10.6 (68.8 %)
+ MRM (3.990-4.218 min, 54 scans) (309.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)280 290 300 310
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1281.0
274.0309.0
+ MRM (321.0 -> 275.0) Benzo Cal03.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 2x10
012345678
lorazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Ratio = 36.9 (96.5 %)
+ MRM (4.023-4.240 min, 51 scans) (321.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)240 260 280 300 320
Coun
ts 4x10
00.10.20.30.40.50.60.70.80.9
1275.0
229.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal03.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 2x10
00.5
1
1.52
2.5
3
desalkylflurazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.20.4
0.60.8
1
1.21.4
289.0 -> 226.0, 289.0 -> 261.0Ratio = 35.6 (124.1 %)
+ MRM (4.438-4.688 min, 59 scans) (289.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 3x10
0
1
2
3
4
5
6
7
226.0
261.0
289.0
![Page 5: [XLS] · Web viewISTD Response Schema Parameter Type Value Comment Include Header Boolean Include Footer Landscape Fit to Integer Page(s) wide Page(s) tall Hide ID Columns Use Options](https://reader042.dokumen.tips/reader042/viewer/2022022523/5b3284cd7f8b9aae458c0abf/html5/page/5.jpg)
Quantitative Analysis Sample Report
document.xlsx Page 5 of 48 Printed at: 05:52:08 on: 05/09/2023
temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal03.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 2x10
012
3456
7temazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Ratio = 29.8 (99.6 %)
+ MRM (4.561-4.776 min, 51 scans) (301.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)175 200 225 250 275 300
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
1.2
255.0
177.0
301.0
+ MRM (300.0 -> 283.0) Benzo Cal03.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 2x10
00.5
11.5
22.5
33.5
chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
300.0 -> 283.0, 300.0 -> 227.0Ratio = 36.6 (100.1 %)
+ MRM (5.265-5.575 min, 73 scans) (300.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 3x10
0
1
2
3
4
5
6
283.0
227.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal03.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 2x10
0
1
2
3
4
5nordiazepam
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
271.0 -> 140.0, 271.0 -> 165.0Ratio = 49.0 (101.7 %)
+ MRM (5.675-5.906 min, 54 scans) (271.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 4x10
00.10.20.30.40.50.60.70.80.9
140.0
165.0
271.0
+ MRM (326.0 -> 291.0) Benzo Cal03.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 2x10
0
0.5
1
1.5
2
2.5
midazolam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
1.2
326.0 -> 291.0, 326.0 -> 249.0Ratio = 29.4 (86.8 %)
+ MRM (6.218-6.483 min, 62 scans) (326.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)260 280 300 320
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
1.2291.0
249.0
326.0
![Page 6: [XLS] · Web viewISTD Response Schema Parameter Type Value Comment Include Header Boolean Include Footer Landscape Fit to Integer Page(s) wide Page(s) tall Hide ID Columns Use Options](https://reader042.dokumen.tips/reader042/viewer/2022022523/5b3284cd7f8b9aae458c0abf/html5/page/6.jpg)
Quantitative Analysis Sample Report
document.xlsx Page 6 of 48 Printed at: 05:52:08 on: 05/09/2023
diazepam+ MRM (285.0 -> 222.0) Benzo Cal03.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 2x10
0
0.5
1
1.5
2
2.53
diazepam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Ratio = 76.3 (92.1 %)
+ MRM (6.339-6.545 min, 49 scans) (285.0 -> **) Benzo Cal03.d
Mass-to-Charge (m/z)220 240 260 280
Coun
ts 4x10
00.10.20.30.40.50.60.70.8
222.0
257.0
285.0
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Quantitative Analysis Sample Report
document.xlsx Page 7 of 48 Printed at: 05:52:08 on: 05/09/2023
Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal04.d Sample Name 50 StdSample Type Calibration Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 11:00Position P1-F4 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Units
7-amino clonazepam 0.982 5192 4417 1.1754 50.4860 ng/ml
1.178 2288 4417 0.5181 48.4112 ng/ml
zopiclone 2.467 8725 4417 1.9755 48.0629 ng/mlclonazepam D4-clonazepam 3.192 10363 8909 1.1632 51.4854 ng/mlflunitrazepam D4-clonazepam 3.287 11983 8909 1.3451 48.2786 ng/ml
alpha-OH alprazolam D4-clonazepam 3.513 5325 8909 0.5977 48.6334 ng/mltriazolam D5-temazepam 3.905 2560 13606 0.1882 52.0534 ng/mloxazepam D5-oxazepam 4.170 9590 10731 0.8937 48.3550 ng/mlflurazepam D5-oxazepam 4.031 7397 10731 0.6893 50.2325 ng/mlalprazolam D5-alprazolam 4.058 7077 6858 1.0320 48.9314 ng/mllorazepam D5-oxazepam 4.110 7536 10731 0.7023 50.1603 ng/ml
desalkylflurazepam D5-temazepam 4.540 4287 13606 0.3151 49.0808 ng/mltemazepam D5-temazepam 4.658 7433 13606 0.5463 49.6216 ng/mlchlordiazepoxide D5-nordiazepam 5.404 4330 4931 0.8780 43.8520 ng/mlnordiazepam D5-nordiazepam 5.831 7264 4931 1.4729 45.0893 ng/mlmidazolam D5-nordiazepam 6.324 4407 4931 0.8937 45.3001 ng/mldiazepam D5-nordiazepam 6.449 3252 4931 0.6594 45.6232 ng/ml
D4-7-amino clonazepam
7-amino flunitrazepam
D4-7-amino clonazepamD4-7-amino clonazepam
+ TIC MRM (** -> **) Benzo Cal04.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 3x10
-0.50
0.51
1.52
2.53
3.54
4.55
5.5
![Page 8: [XLS] · Web viewISTD Response Schema Parameter Type Value Comment Include Header Boolean Include Footer Landscape Fit to Integer Page(s) wide Page(s) tall Hide ID Columns Use Options](https://reader042.dokumen.tips/reader042/viewer/2022022523/5b3284cd7f8b9aae458c0abf/html5/page/8.jpg)
Quantitative Analysis Sample Report
document.xlsx Page 8 of 48 Printed at: 05:52:08 on: 05/09/2023
Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal04.d
Acquisition Time (min)0.5 1 1.5
Coun
ts 3x10
00.20.40.60.8
11.21.41.6
7-amino clonazepam
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Ratio = 79.2 (95.9 %)
+ MRM (0.934-1.081 min, 35 scans) (286.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 4x10
00.20.40.60.8
11.21.41.61.8
121.0
222.0
286.0
+ MRM (284.0 -> 226.0) Benzo Cal04.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts 2x10
01234567
7-amino flunitrazepam
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Ratio = 83.8 (107.3 %)
+ MRM (1.119-1.315 min, 46 scans) (284.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 3x10
012345678
226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal04.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts 3x10
0
0.5
1
1.5
2
2.5zopiclone
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Ratio = 60.6 (107.1 %)
+ MRM (2.392-2.596 min, 48 scans) (389.1 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)250 300 350
Coun
ts 4x10
00.5
11.5
22.5
33.5
4245.1
217.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal04.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
00.250.5
0.751
1.251.5
1.752
clonazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Ratio = 20.2 (87.7 %)
+ MRM (3.110-3.440 min, 78 scans) (316.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
3
270.0
214.0
316.0
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Quantitative Analysis Sample Report
document.xlsx Page 9 of 48 Printed at: 05:52:08 on: 05/09/2023
flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal04.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
0
0.5
1
1.5
2
2.5flunitrazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
314.0 -> 268.0, 314.0 -> 239.0Ratio = 52.4 (105.2 %)
+ MRM (3.162-3.457 min, 69 scans) (314.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 4x10
0
0.5
1
1.5
22.5
3
3.5
268.0
239.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal04.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
1
1.2alpha-OH alprazolam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Ratio = 36.0 (107.4 %)
+ MRM (3.414-3.667 min, 60 scans) (325.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 4x10
00.20.40.60.8
11.21.41.6
297.0
216.0
325.0
+ MRM (343.0 -> 308.0) Benzo Cal04.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 2x10
0
1
2
3
4
5
triazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
343.0 -> 308.0, 343.0 -> 239.0Ratio = 78.4 (89.8 %)
+ MRM (3.822-4.027 min, 49 scans) (343.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts 3x10
0
1
2
3
4
5
6
7308.0
239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal04.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.250.5
0.751
1.251.5
1.752
oxazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
287.0 -> 241.0, 287.0 -> 269.0Ratio = 30.9 (108.3 %)
+ MRM (4.054-4.277 min, 53 scans) (287.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)240 250 260 270 280
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
241.0
269.0
287.0
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Quantitative Analysis Sample Report
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flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal04.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.2
0.4
0.60.8
1
1.21.4
flurazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
388.0 -> 315.0, 388.0 -> 288.0Ratio = 14.9 (102.9 %)
+ MRM (3.918-4.158 min, 57 scans) (388.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)300 320 340 360 380
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.752
315.0
288.0
388.0
+ MRM (309.0 -> 281.0) Benzo Cal04.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.20.40.60.8
11.21.4
alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.20.40.6
0.81
1.2
1.4
309.0 -> 281.0, 309.0 -> 274.0Ratio = 21.2 (136.9 %)
+ MRM (3.961-4.241 min, 66 scans) (309.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)280 290 300 310
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.752
281.0
274.0
309.0
+ MRM (321.0 -> 275.0) Benzo Cal04.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.20.40.60.8
11.21.4
lorazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Ratio = 36.7 (96.0 %)
+ MRM (3.988-4.234 min, 58 scans) (321.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)240 260 280 300 320
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.752
275.0
229.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal04.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 2x10
01234567
desalkylflurazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
289.0 -> 226.0, 289.0 -> 261.0Ratio = 36.4 (127.0 %)
+ MRM (4.425-4.746 min, 76 scans) (289.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 4x10
00.20.40.6
0.81
1.21.4
226.0
261.0
289.0
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Quantitative Analysis Sample Report
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temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal04.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
00.2
0.40.60.8
1
1.21.4
temazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Ratio = 29.3 (98.1 %)
+ MRM (4.535-4.820 min, 67 scans) (301.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)175 200 225 250 275 300
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
255.0
177.0
301.0
+ MRM (300.0 -> 283.0) Benzo Cal04.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 2x10
01
23
45
67
chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
300.0 -> 283.0, 300.0 -> 227.0Ratio = 37.9 (103.6 %)
+ MRM (5.258-5.563 min, 72 scans) (300.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
1.2
283.0
227.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal04.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
1nordiazepam
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
271.0 -> 140.0, 271.0 -> 165.0Ratio = 49.3 (102.2 %)
+ MRM (5.703-5.962 min, 61 scans) (271.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.752
140.0
165.0
271.0
+ MRM (326.0 -> 291.0) Benzo Cal04.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 2x10
012
34
5
6
midazolam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
326.0 -> 291.0, 326.0 -> 249.0Ratio = 30.7 (90.5 %)
+ MRM (6.201-6.454 min, 60 scans) (326.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)260 280 300 320
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.752
2.25
291.0
249.0
326.0
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Quantitative Analysis Sample Report
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diazepam+ MRM (285.0 -> 222.0) Benzo Cal04.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 2x10
01234567
diazepam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Ratio = 80.2 (96.8 %)
+ MRM (6.351-6.568 min, 51 scans) (285.0 -> **) Benzo Cal04.d
Mass-to-Charge (m/z)220 240 260 280
Coun
ts 4x10
00.20.40.60.8
11.21.41.61.8
222.0
257.0
285.0
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Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal05.d Sample Name 125 StdSample Type Calibration Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 11:11Position P1-F5 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Units
7-amino clonazepam 0.982 12941 4173 3.1011 125.4191 ng/ml
1.176 6704 4173 1.6064 136.0114 ng/ml
zopiclone 2.465 23794 4173 5.7017 130.6195 ng/mlclonazepam D4-clonazepam 3.188 25046 8960 2.7953 118.8979 ng/mlflunitrazepam D4-clonazepam 3.286 33023 8960 3.6855 127.1428 ng/ml
alpha-OH alprazolam D4-clonazepam 3.509 14188 8960 1.5835 123.3556 ng/mltriazolam D5-temazepam 3.898 5947 13101 0.4539 122.8973 ng/mloxazepam D5-oxazepam 4.165 25498 11176 2.2815 125.7753 ng/mlflurazepam D5-oxazepam 4.018 19398 11176 1.7357 124.3526 ng/mlalprazolam D5-alprazolam 4.054 19133 6538 2.9265 130.7423 ng/mllorazepam D5-oxazepam 4.106 19938 11176 1.7841 123.0284 ng/ml
desalkylflurazepam D5-temazepam 4.534 10592 13101 0.8085 122.2640 ng/mltemazepam D5-temazepam 4.650 17999 13101 1.3739 122.1752 ng/mlchlordiazepoxide D5-nordiazepam 5.392 11594 4723 2.4551 128.0163 ng/mlnordiazepam D5-nordiazepam 5.814 19918 4723 4.2177 120.5695 ng/mlmidazolam D5-nordiazepam 6.327 11852 4723 2.5097 126.9027 ng/mldiazepam D5-nordiazepam 6.437 8651 4723 1.8318 125.5977 ng/ml
D4-7-amino clonazepam
7-amino flunitrazepam
D4-7-amino clonazepamD4-7-amino clonazepam
+ TIC MRM (** -> **) Benzo Cal05.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 4x10
-0.10
0.10.20.30.40.50.60.70.80.9
11.1
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Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal05.d
Acquisition Time (min)0.5 1 1.5
Coun
ts 3x10
00.5
11.5
22.5
33.5
4
7-amino clonazepam
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Ratio = 85.4 (103.5 %)
+ MRM (0.927-1.094 min, 40 scans) (286.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 4x10
00.5
11.5
22.5
33.5
44.5
121.0
222.0
286.0
+ MRM (284.0 -> 226.0) Benzo Cal05.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts 3x10
00.20.40.60.8
11.21.41.61.8
7-amino flunitrazepam
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Ratio = 67.5 (86.5 %)
+ MRM (1.011-1.373 min, 85 scans) (284.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.752
2.25
226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal05.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts 3x10
0123
4567
zopiclone
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Ratio = 55.9 (98.8 %)
+ MRM (2.378-2.603 min, 53 scans) (389.1 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)250 300 350
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
245.1
217.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal05.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
0
1
2
3
4
5
clonazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Ratio = 24.3 (105.7 %)
+ MRM (3.101-3.359 min, 61 scans) (316.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 4x10
012345678
270.0
214.0
316.0
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flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal05.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
01
2345
67
flunitrazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
314.0 -> 268.0, 314.0 -> 239.0Ratio = 53.1 (106.5 %)
+ MRM (3.142-3.498 min, 84 scans) (314.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1268.0
239.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal05.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
0
0.5
1
1.5
2
2.5
3alpha-OH alprazolam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Ratio = 32.0 (95.4 %)
+ MRM (3.403-3.672 min, 63 scans) (325.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 4x10
00.5
11.5
22.5
33.5
44.5
297.0
216.0
325.0
+ MRM (343.0 -> 308.0) Benzo Cal05.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
1
1.2
triazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
343.0 -> 308.0, 343.0 -> 239.0Ratio = 99.5 (113.9 %)
+ MRM (3.807-4.050 min, 57 scans) (343.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts 4x10
00.20.40.60.8
11.21.41.6
308.0239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal05.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
1
2
3
4
5oxazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
287.0 -> 241.0, 287.0 -> 269.0Ratio = 29.6 (103.6 %)
+ MRM (4.054-4.317 min, 62 scans) (287.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)240 250 260 270 280
Coun
ts 4x10
0
1
2
3
4
5
6
7241.0
269.0
287.0
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Quantitative Analysis Sample Report
document.xlsx Page 16 of 48 Printed at: 05:52:08 on: 05/09/2023
flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal05.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
0.5
1
1.52
2.53
flurazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
388.0 -> 315.0, 388.0 -> 288.0Ratio = 16.7 (115.7 %)
+ MRM (3.911-4.243 min, 78 scans) (388.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)300 320 340 360 380
Coun
ts 4x10
0
1
2
3
4
5
315.0
288.0
388.0
+ MRM (309.0 -> 281.0) Benzo Cal05.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.5
11.5
22.5
33.5
alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
309.0 -> 281.0, 309.0 -> 274.0Ratio = 16.2 (104.7 %)
+ MRM (3.948-4.207 min, 61 scans) (309.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)280 290 300 310
Coun
ts 4x10
0
1
2
3
4
5
281.0
274.0
309.0
+ MRM (321.0 -> 275.0) Benzo Cal05.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.5
11.5
22.5
33.5
4lorazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Ratio = 38.0 (99.4 %)
+ MRM (4.005-4.245 min, 57 scans) (321.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)240 260 280 300 320
Coun
ts 4x10
0
1
2
3
4
5
275.0
229.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal05.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
00.20.40.60.8
11.21.41.6
desalkylflurazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
1.2
289.0 -> 226.0, 289.0 -> 261.0Ratio = 31.2 (108.7 %)
+ MRM (4.400-4.800 min, 94 scans) (289.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 4x10
00.5
11.5
22.5
33.5
4226.0
261.0
289.0
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Quantitative Analysis Sample Report
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temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal05.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
00.5
11.5
22.5
33.5
temazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Ratio = 31.1 (104.2 %)
+ MRM (4.508-4.798 min, 68 scans) (301.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)175 200 225 250 275 300
Coun
ts 4x10
0
1
2
3
4
5
6
255.0
177.0
301.0
+ MRM (300.0 -> 283.0) Benzo Cal05.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 3x10
00.250.5
0.751
1.251.5
1.75
chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
300.0 -> 283.0, 300.0 -> 227.0Ratio = 36.0 (98.6 %)
+ MRM (5.257-5.575 min, 75 scans) (300.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
3
283.0
227.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal05.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 3x10
0
0.5
1
1.5
2
2.5
nordiazepam
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
271.0 -> 140.0, 271.0 -> 165.0Ratio = 44.2 (91.7 %)
+ MRM (5.641-6.069 min, 101 scans) (271.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 4x10
0
1
2
3
4
5
140.0
165.0
271.0
+ MRM (326.0 -> 291.0) Benzo Cal05.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 3x10
00.20.40.60.8
11.21.41.6
midazolam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
326.0 -> 291.0, 326.0 -> 249.0Ratio = 30.1 (88.8 %)
+ MRM (6.195-6.509 min, 74 scans) (326.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)260 280 300 320
Coun
ts 4x10
0
1
2
3
4
5
6
291.0
249.0
326.0
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Quantitative Analysis Sample Report
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diazepam+ MRM (285.0 -> 222.0) Benzo Cal05.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 3x10
00.250.5
0.751
1.251.5
1.75
diazepam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Ratio = 82.1 (99.1 %)
+ MRM (6.351-6.594 min, 58 scans) (285.0 -> **) Benzo Cal05.d
Mass-to-Charge (m/z)220 240 260 280
Coun
ts 4x10
00.5
11.5
22.5
33.5
44.5
222.0
257.0
285.0
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Quantitative Analysis Sample Report
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Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal06.d Sample Name 250 StdSample Type Calibration Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 11:21Position P1-F6 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Units
7-amino clonazepam 0.984 28338 4243 6.6792 246.5235 ng/ml
1.179 12600 4243 2.9698 236.0636 ng/ml
zopiclone 2.470 48755 4243 11.4914 247.2174 ng/mlclonazepam D4-clonazepam 3.196 56764 9490 5.9811 250.4847 ng/mlflunitrazepam D4-clonazepam 3.292 69174 9490 7.2888 248.5578 ng/ml
alpha-OH alprazolam D4-clonazepam 3.517 32945 9490 3.4714 266.4532 ng/mltriazolam D5-temazepam 3.906 12184 12424 0.9806 263.2900 ng/mloxazepam D5-oxazepam 4.175 56020 11064 5.0634 255.3699 ng/mlflurazepam D5-oxazepam 4.025 41757 11064 3.7742 250.6731 ng/mlalprazolam D5-alprazolam 4.064 41287 7240 5.7023 242.2913 ng/mllorazepam D5-oxazepam 4.111 43776 11064 3.9568 254.8781 ng/ml
desalkylflurazepam D5-temazepam 4.545 22790 12424 1.8343 264.0711 ng/mltemazepam D5-temazepam 4.662 38157 12424 3.0711 260.7158 ng/mlchlordiazepoxide D5-nordiazepam 5.400 23692 4113 5.7596 281.6412 ng/mlnordiazepam D5-nordiazepam 5.833 40513 4113 9.8489 275.4278 ng/mlmidazolam D5-nordiazepam 6.315 24202 4113 5.8837 275.7316 ng/mldiazepam D5-nordiazepam 6.447 18256 4113 4.4382 277.8351 ng/ml
D4-7-amino clonazepam
7-amino flunitrazepam
D4-7-amino clonazepamD4-7-amino clonazepam
+ TIC MRM (** -> **) Benzo Cal06.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 4x10
-0.20
0.20.40.60.8
11.21.41.61.8
22.22.4
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Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal06.d
Acquisition Time (min)0.5 1 1.5
Coun
ts 4x10
00.10.20.30.40.50.60.70.80.9
7-amino clonazepam
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Ratio = 82.6 (100.0 %)
+ MRM (0.933-1.166 min, 55 scans) (286.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1121.0
222.0
286.0
+ MRM (284.0 -> 226.0) Benzo Cal06.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts 3x10
00.5
11.5
22.5
33.5
7-amino flunitrazepam
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Ratio = 82.4 (105.5 %)
+ MRM (1.126-1.337 min, 50 scans) (284.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 4x10
00.5
11.5
22.5
33.5
44.5
226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal06.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts 4x10
00.20.40.60.8
11.21.4
zopiclone
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Ratio = 56.6 (100.0 %)
+ MRM (2.382-2.596 min, 51 scans) (389.1 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)250 300 350
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
2.25245.1
217.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal06.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
clonazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Ratio = 23.0 (100.0 %)
+ MRM (3.089-3.511 min, 99 scans) (316.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 5x10
00.20.40.60.8
11.21.41.61.8
270.0
214.0
316.0
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Quantitative Analysis Sample Report
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flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal06.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 4x10
00.20.40.60.8
11.21.4
flunitrazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
314.0 -> 268.0, 314.0 -> 239.0Ratio = 50.5 (101.3 %)
+ MRM (3.127-3.472 min, 81 scans) (314.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
268.0
239.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal06.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
01234567
alpha-OH alprazolam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Ratio = 33.5 (100.0 %)
+ MRM (3.405-3.657 min, 59 scans) (325.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1297.0
216.0
325.0
+ MRM (343.0 -> 308.0) Benzo Cal06.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
0.5
1
1.5
2
triazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
343.0 -> 308.0, 343.0 -> 239.0Ratio = 87.3 (100.0 %)
+ MRM (3.819-4.113 min, 69 scans) (343.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
3
3.5308.0
239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal06.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
oxazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
287.0 -> 241.0, 287.0 -> 269.0Ratio = 28.6 (100.0 %)
+ MRM (4.031-4.317 min, 67 scans) (287.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)240 250 260 270 280
Coun
ts 5x10
00.20.40.6
0.81
1.21.4
241.0
269.0
287.0
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Quantitative Analysis Sample Report
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flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal06.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
01234567
flurazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
388.0 -> 315.0, 388.0 -> 288.0Ratio = 14.4 (100.0 %)
+ MRM (3.910-4.220 min, 73 scans) (388.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)300 320 340 360 380
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
1.2315.0
288.0
388.0
+ MRM (309.0 -> 281.0) Benzo Cal06.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
012345678
alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
309.0 -> 281.0, 309.0 -> 274.0Ratio = 15.5 (100.0 %)
+ MRM (3.956-4.264 min, 73 scans) (309.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)280 290 300 310
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
281.0
274.0
309.0
+ MRM (321.0 -> 275.0) Benzo Cal06.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 4x10
00.10.20.30.40.50.60.70.80.9
lorazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Ratio = 38.2 (100.0 %)
+ MRM (3.994-4.314 min, 75 scans) (321.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)240 260 280 300 320
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
1.2275.0
229.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal06.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
00.5
11.5
22.5
33.5
desalkylflurazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
289.0 -> 226.0, 289.0 -> 261.0Ratio = 28.7 (100.0 %)
+ MRM (4.431-4.835 min, 95 scans) (289.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 4x10
012345678
226.0
261.0
289.0
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Quantitative Analysis Sample Report
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temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal06.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
01234567
temazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Ratio = 29.9 (100.0 %)
+ MRM (4.539-4.798 min, 61 scans) (301.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)175 200 225 250 275 300
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
1.2
255.0
177.0
301.0
+ MRM (300.0 -> 283.0) Benzo Cal06.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 3x10
00.5
11.5
22.5
33.5
chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
300.0 -> 283.0, 300.0 -> 227.0Ratio = 36.5 (100.0 %)
+ MRM (5.251-5.586 min, 79 scans) (300.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 4x10
0
1
2
3
4
5
6
283.0
227.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal06.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 3x10
0
1
2
3
4
5
nordiazepam
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
271.0 -> 140.0, 271.0 -> 165.0Ratio = 48.2 (100.0 %)
+ MRM (5.676-6.041 min, 86 scans) (271.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
140.0
165.0
271.0
+ MRM (326.0 -> 291.0) Benzo Cal06.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 3x10
0
0.5
1
1.52
2.5
3
midazolam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
326.0 -> 291.0, 326.0 -> 249.0Ratio = 33.9 (100.0 %)
+ MRM (6.184-6.491 min, 72 scans) (326.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)260 280 300 320
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
1.2
291.0
249.0
326.0
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Quantitative Analysis Sample Report
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diazepam+ MRM (285.0 -> 222.0) Benzo Cal06.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 3x10
00.5
11.5
22.5
33.5
4diazepam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Ratio = 82.8 (100.0 %)
+ MRM (6.324-6.638 min, 74 scans) (285.0 -> **) Benzo Cal06.d
Mass-to-Charge (m/z)220 240 260 280
Coun
ts 5x10
00.10.20.30.40.50.60.70.80.9
1222.0
257.0
285.0
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Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal07.d Sample Name 500 StdSample Type Calibration Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 11:31Position P1-F7 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Units
7-amino clonazepam 0.983 60068 3717 16.1584 503.0092 ng/ml
1.180 27273 3717 7.3365 508.6383 ng/ml
zopiclone 2.471 97125 3717 26.1271 499.4428 ng/mlclonazepam D4-clonazepam 3.200 119448 9637 12.3948 515.3953 ng/mlflunitrazepam D4-clonazepam 3.295 144052 9637 14.9478 506.6413 ng/ml
alpha-OH alprazolam D4-clonazepam 3.518 61553 9637 6.3872 487.4619 ng/mltriazolam D5-temazepam 3.910 22776 12795 1.7801 476.3922 ng/mloxazepam D5-oxazepam 4.177 119797 10219 11.7231 495.0952 ng/mlflurazepam D5-oxazepam 4.024 89279 10219 8.7367 499.7925 ng/mlalprazolam D5-alprazolam 4.065 84446 6451 13.0904 504.1232 ng/mllorazepam D5-oxazepam 4.114 88618 10219 8.6720 497.1498 ng/ml
desalkylflurazepam D5-temazepam 4.548 46946 12795 3.6690 491.2347 ng/mltemazepam D5-temazepam 4.660 80203 12795 6.2682 493.4810 ng/mlchlordiazepoxide D5-nordiazepam 5.399 48302 4463 10.8233 479.3994 ng/mlnordiazepam D5-nordiazepam 5.827 83063 4463 18.6123 516.4187 ng/mlmidazolam D5-nordiazepam 6.319 51236 4463 11.4809 482.3231 ng/mldiazepam D5-nordiazepam 6.443 38828 4463 8.7005 481.8240 ng/ml
D4-7-amino clonazepam
7-amino flunitrazepam
D4-7-amino clonazepamD4-7-amino clonazepam
+ TIC MRM (** -> **) Benzo Cal07.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 4x10
-0.5
00.5
11.5
22.5
33.5
44.5
5
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Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal07.d
Acquisition Time (min)0.5 1 1.5
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.75
7-amino clonazepam
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Ratio = 74.5 (90.2 %)
+ MRM (0.836-1.224 min, 91 scans) (286.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
121.0
222.0
286.0
+ MRM (284.0 -> 226.0) Benzo Cal07.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts 3x10
01234567
7-amino flunitrazepam
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Ratio = 80.3 (102.9 %)
+ MRM (1.126-1.498 min, 87 scans) (284.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 5x10
00.10.20.30.40.50.60.70.80.9
1226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal07.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
zopiclone
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Ratio = 54.0 (95.4 %)
+ MRM (2.382-2.585 min, 48 scans) (389.1 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)250 300 350
Coun
ts 5x10
00.5
11.5
22.5
33.5
44.5
245.1
217.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal07.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 4x10
0
0.5
1
1.5
2
2.5clonazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Ratio = 21.5 (93.4 %)
+ MRM (3.095-3.506 min, 97 scans) (316.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 5x10
0
0.5
1
1.5
2
2.5
3
3.5
270.0
214.0
316.0
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flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal07.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
3
flunitrazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
314.0 -> 268.0, 314.0 -> 239.0Ratio = 51.0 (102.3 %)
+ MRM (3.137-3.538 min, 94 scans) (314.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 5x10
00.5
11.5
22.5
33.5
44.5
268.0
239.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal07.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 4x10
00.20.40.60.8
11.21.4
alpha-OH alprazolam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Ratio = 31.1 (92.9 %)
+ MRM (3.397-3.662 min, 62 scans) (325.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 5x10
00.20.40.60.8
11.21.41.61.8
297.0
216.0
325.0
+ MRM (343.0 -> 308.0) Benzo Cal07.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
1
2
3
4
triazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
343.0 -> 308.0, 343.0 -> 239.0Ratio = 98.2 (112.5 %)
+ MRM (3.817-4.073 min, 60 scans) (343.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts 4x10
0
1
2
3
4
5
6
7308.0
239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal07.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 4x10
0
0.5
1
1.5
2
2.5oxazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
287.0 -> 241.0, 287.0 -> 269.0Ratio = 27.4 (95.9 %)
+ MRM (4.043-4.322 min, 66 scans) (287.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)240 250 260 270 280
Coun
ts 5x10
0
0.5
1
1.5
2
2.5
3
241.0
269.0
287.0
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flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal07.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 4x10
00.20.40.60.8
11.21.41.6
flurazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
388.0 -> 315.0, 388.0 -> 288.0Ratio = 14.2 (98.3 %)
+ MRM (3.914-4.169 min, 60 scans) (388.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)300 320 340 360 380
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
2.252.5
315.0
288.0
388.0
+ MRM (309.0 -> 281.0) Benzo Cal07.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 4x10
00.20.40.60.8
11.21.41.6
alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
309.0 -> 281.0, 309.0 -> 274.0Ratio = 15.2 (98.3 %)
+ MRM (3.965-4.212 min, 58 scans) (309.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)280 290 300 310
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
2.25
281.0
274.0
309.0
+ MRM (321.0 -> 275.0) Benzo Cal07.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.75
lorazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Ratio = 39.6 (103.6 %)
+ MRM (3.984-4.297 min, 74 scans) (321.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)240 260 280 300 320
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
2.252.5
275.0
229.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal07.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
012345678
desalkylflurazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
289.0 -> 226.0, 289.0 -> 261.0Ratio = 31.6 (110.3 %)
+ MRM (4.426-4.862 min, 102 scans) (289.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 5x10
00.20.40.60.8
11.21.41.6
226.0
261.0
289.0
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temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal07.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 4x10
00.20.40.60.8
11.21.41.6
temazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Ratio = 28.0 (93.8 %)
+ MRM (4.521-4.834 min, 74 scans) (301.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)175 200 225 250 275 300
Coun
ts 5x10
0
0.5
1
1.5
2
2.5
255.0
177.0
301.0
+ MRM (300.0 -> 283.0) Benzo Cal07.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 3x10
012345678
chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
300.0 -> 283.0, 300.0 -> 227.0Ratio = 36.7 (100.4 %)
+ MRM (5.262-5.592 min, 78 scans) (300.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 5x10
0
0.2
0.4
0.6
0.8
1
1.2
1.4283.0
227.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal07.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
nordiazepam
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
271.0 -> 140.0, 271.0 -> 165.0Ratio = 51.5 (107.0 %)
+ MRM (5.668-6.075 min, 95 scans) (271.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
2.25
140.0
165.0
271.0
+ MRM (326.0 -> 291.0) Benzo Cal07.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 3x10
01234567
midazolam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
326.0 -> 291.0, 326.0 -> 249.0Ratio = 31.8 (93.9 %)
+ MRM (6.181-6.494 min, 74 scans) (326.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)260 280 300 320
Coun
ts 5x10
0
0.5
1
1.5
2
2.5
291.0
249.0
326.0
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diazepam+ MRM (285.0 -> 222.0) Benzo Cal07.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 4x10
00.10.20.30.40.50.60.70.8
diazepam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Ratio = 79.1 (95.5 %)
+ MRM (6.325-6.702 min, 89 scans) (285.0 -> **) Benzo Cal07.d
Mass-to-Charge (m/z)220 240 260 280
Coun
ts 5x10
00.250.5
0.751
1.251.5
1.752
222.0
257.0
285.0
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Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal02.d Sample Name BlankSample Type Blank Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 10:40Position P1-F2 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Unitsclonazepam D4-clonazepam 3.301 48 8516 0.0056 3.6723 ng/mlflunitrazepam D4-clonazepam 3.311 204 8516 0.0240 3.7616 ng/ml
alpha-OH alprazolam D4-clonazepam 2.958 25 8516 0.0029 3.5495 ng/mlflurazepam D5-oxazepam 4.012 34 10196 0.0033 0.0000 ng/mlalprazolam D5-alprazolam 3.953 16 6786 0.0024 2.1557 ng/mllorazepam D5-oxazepam 4.225 14 10196 0.0014 0.0000 ng/ml
desalkylflurazepam D5-temazepam 4.614 17 12490 0.0014 0.5431 ng/mlchlordiazepoxide D5-nordiazepam 5.409 14 3974 0.0034 0.0000 ng/mlnordiazepam D5-nordiazepam 5.239 7 3974 0.0017 4.6309 ng/mlmidazolam D5-nordiazepam 6.531 13 3974 0.0034 0.0000 ng/mldiazepam D5-nordiazepam 6.529 39 3974 0.0099 0.0000 ng/ml
+ TIC MRM (** -> **) Benzo Cal02.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 3x10
-0.20
0.20.40.60.8
11.21.41.61.8
22.22.42.6
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Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal02.d
Acquisition Time (min)0.5 1 1.5
Coun
ts
0
1
2
3
4
5
6
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Not Found
+ MRM (0.000-1.984 min, 464 scans) (286.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 2x10
00.10.20.30.40.50.60.70.80.9
222.0121.0
286.0
+ MRM (284.0 -> 226.0) Benzo Cal02.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts
012345678
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Not Found
+ MRM (0.179-2.179 min, 468 scans) (284.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 2x10
0
0.2
0.40.6
0.8
1
1.2
1.4226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal02.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Not Found
+ MRM (1.470-3.470 min, 468 scans) (389.1 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)250 300 350
Coun
ts
012
34
56
7
8 217.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal02.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
00.20.40.60.8
11.21.41.61.8
clonazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Not Found
+ MRM (3.268-3.334 min, 16 scans) (316.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)270 280 290 300 310
Coun
ts 2x10
0
0.2
0.4
0.6
0.8
1
1.21.4
270.0
316.0
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Quantitative Analysis Sample Report
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flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal02.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
0
1
2
3
4
5
6flunitrazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
314.0 -> 268.0, 314.0 -> 239.0Ratio = 18.8 (37.7 %)
+ MRM (3.264-3.381 min, 28 scans) (314.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 2x10
0
1
2
3
4
5
6
268.0
239.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal02.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
0
0.2
0.4
0.6
0.8
alpha-OH alprazolam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Not Found
+ MRM (2.920-2.981 min, 15 scans) (325.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)300 305 310 315 320 325
Coun
ts 1x10
0
1
23
4
56
7
297.0
325.0
+ MRM (343.0 -> 308.0) Benzo Cal02.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
343.0 -> 308.0, 343.0 -> 239.0Not Found
+ MRM (2.906-4.906 min, 468 scans) (343.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts 1x10
00.20.40.60.8
11.21.41.6
239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal02.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
287.0 -> 241.0, 287.0 -> 269.0Not Found
+ MRM (3.000-5.000 min, 468 scans) (287.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)270 275 280 285
Coun
ts 1x10
0
1
2
3
4
5
6
269.0
287.0
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flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal02.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 1x10
0
0.2
0.4
0.6
0.8
1
flurazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
388.0 -> 315.0, 388.0 -> 288.0Not Found
+ MRM (3.986-4.044 min, 14 scans) (388.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)320 340 360 380
Coun
ts 2x10
00.10.20.30.40.50.60.70.80.9
315.0
388.0
+ MRM (309.0 -> 281.0) Benzo Cal02.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts
0
1
2
3
4
5
6 alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
309.0 -> 281.0, 309.0 -> 274.0Not Found
+ MRM (3.927-3.990 min, 15 scans) (309.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)280 285 290 295 300 305 310
Coun
ts 1x10
00.5
11.5
22.5
33.5
44.5
281.0
309.0
+ MRM (321.0 -> 275.0) Benzo Cal02.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts
0
1
2
3
4
5 lorazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Not Found
+ MRM (4.205-4.268 min, 15 scans) (321.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)280 290 300 310 320
Coun
ts 1x10
00.5
11.5
22.5
33.5
4275.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal02.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts
012345678 desalkylflurazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
1
2
3
4
5
6
289.0 -> 226.0, 289.0 -> 261.0Ratio = 339.0 (1181.6 %)
+ MRM (4.590-4.643 min, 13 scans) (289.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 1x10
012345678
261.0
226.0
289.0
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temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal02.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts
0
0.2
0.4
0.6
0.8
1
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Not Found
+ MRM (3.662-5.662 min, 468 scans) (301.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)260 270 280 290 300
Coun
ts
0
12
34
56
7
8 255.0
301.0
+ MRM (300.0 -> 283.0) Benzo Cal02.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 1x10
0
0.2
0.4
0.6
0.8
1
1.2
chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.5
1
1.5
2
300.0 -> 283.0, 300.0 -> 227.0Not Found
+ MRM (5.372-5.440 min, 16 scans) (300.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)285 290 295 300
Coun
ts 1x10
0
0.5
1
1.5
22.5
3
3.5
283.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal02.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts
0
0.5
1
1.5
2
2.5
3 nordiazepam
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
271.0 -> 140.0, 271.0 -> 165.0Not Found
+ MRM (5.208-5.270 min, 15 scans) (271.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 1x10
00.20.40.60.8
11.21.41.61.8
140.0
271.0
+ MRM (326.0 -> 291.0) Benzo Cal02.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts
0123456789 midazolam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
326.0 -> 291.0, 326.0 -> 249.0Not Found
+ MRM (6.517-6.552 min, 9 scans) (326.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)290 300 310 320
Coun
ts 1x10
0
1
2
3
4
5
6
7291.0
326.0
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Quantitative Analysis Sample Report
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diazepam+ MRM (285.0 -> 222.0) Benzo Cal02.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 1x10
00.250.5
0.751
1.251.5
1.752
diazepam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Ratio = 87.1 (105.2 %)
+ MRM (6.504-6.565 min, 15 scans) (285.0 -> **) Benzo Cal02.d
Mass-to-Charge (m/z)220 240 260 280
Coun
ts 2x10
00.250.5
0.751
1.251.5
1.752
222.0257.0
285.0
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Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal09.d Sample Name 123456Sample Type Sample Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 12:01Position P1-F9 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Units
zopiclone 2.463 1687 4599 0.3668 10.1229 ng/ml
alpha-OH alprazolam D4-clonazepam 3.507 53 8160 0.0065 3.8224 ng/mloxazepam D5-oxazepam 3.749 43 10428 0.0042 0.0000 ng/mlalprazolam D5-alprazolam 4.058 21422 6522 3.2845 145.6462 ng/mlchlordiazepoxide D5-nordiazepam 5.355 20 4277 0.0046 0.0000 ng/ml
D4-7-amino clonazepam
+ TIC MRM (** -> **) Benzo Cal09.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 3x10
-0.50
0.51
1.52
2.53
3.54
4.55
5.56
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Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal09.d
Acquisition Time (min)0.5 1 1.5
Coun
ts 2x10
0
0.5
1
1.5
2
2.5
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Not Found
+ MRM (0.000-1.984 min, 464 scans) (286.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 3x10
0
0.5
1
1.5
2
2.5
121.0
222.0286.0
+ MRM (284.0 -> 226.0) Benzo Cal09.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts 1x10
0
0.2
0.4
0.6
0.8
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Not Found
+ MRM (0.179-2.179 min, 468 scans) (284.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 2x10
00.20.40.60.8
11.21.41.61.8
2226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal09.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts 2x10
0
1
2
3
4
5zopiclone
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Ratio = 62.5 (110.5 %)
+ MRM (2.392-2.547 min, 37 scans) (389.1 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)250 300 350
Coun
ts 3x10
01
2345
67
245.1
217.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal09.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Not Found
+ MRM (2.196-4.196 min, 468 scans) (316.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)290 300 310 320 330 340
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
316.0
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Quantitative Analysis Sample Report
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flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal09.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
314.0 -> 268.0, 314.0 -> 239.0Not Found
+ MRM (2.282-4.282 min, 468 scans) (314.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)285 290 295 300 305 310 315
Coun
ts 4x10
00.20.40.60.8
11.21.41.61.8
286.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal09.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
00.250.5
0.751
1.251.5
1.75
alpha-OH alprazolam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Ratio = 37.4 (111.5 %)
+ MRM (3.469-3.550 min, 20 scans) (325.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 2x10
00.20.40.60.8
11.21.41.6
297.0
216.0
325.0
+ MRM (343.0 -> 308.0) Benzo Cal09.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
343.0 -> 308.0, 343.0 -> 239.0Not Found
+ MRM (2.906-4.906 min, 468 scans) (343.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts
0
12
34
56
7
8 239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal09.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 1x10
0
1
2
3
4
oxazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
1
2
3
4
5
287.0 -> 241.0, 287.0 -> 269.0Not Found
+ MRM (3.717-3.763 min, 11 scans) (287.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)240 250 260 270 280
Coun
ts 2x10
0
0.2
0.4
0.6
0.8
1
1.2241.0
287.0
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Quantitative Analysis Sample Report
document.xlsx Page 40 of 48 Printed at: 05:52:08 on: 05/09/2023
flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal09.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
388.0 -> 315.0, 388.0 -> 288.0Not Found
+ MRM (3.025-5.025 min, 468 scans) (388.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)360 380 400 420
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
388.0
+ MRM (309.0 -> 281.0) Benzo Cal09.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.5
11.5
22.5
33.5
4alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
309.0 -> 281.0, 309.0 -> 274.0Ratio = 16.1 (103.8 %)
+ MRM (3.938-4.190 min, 59 scans) (309.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)280 290 300 310
Coun
ts 4x10
0
1
2
3
4
5
6281.0
274.0
309.0
+ MRM (321.0 -> 275.0) Benzo Cal09.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts
00.250.5
0.751
1.251.5
1.752
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Not Found
+ MRM (3.111-5.111 min, 468 scans) (321.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)280 290 300 310 320
Coun
ts 1x10
00.20.40.60.8
11.21.41.6
275.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal09.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 1x10
00.20.40.60.8
11.21.4
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
289.0 -> 226.0, 289.0 -> 261.0Not Found
+ MRM (3.575-5.575 min, 468 scans) (289.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 2x10
0
0.5
1
1.5
2
2.5
3
3.5261.0
226.0289.0
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Quantitative Analysis Sample Report
document.xlsx Page 41 of 48 Printed at: 05:52:08 on: 05/09/2023
temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal09.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Not Found
+ MRM (3.662-5.662 min, 468 scans) (301.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)280 290 300 310 320
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
301.0
+ MRM (300.0 -> 283.0) Benzo Cal09.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts
0
1
2
34
56
7 chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
300.0 -> 283.0, 300.0 -> 227.0Not Found
+ MRM (5.327-5.395 min, 16 scans) (300.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)285 290 295 300
Coun
ts 1x10
0
1
2
3
4
5
283.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal09.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts
0
0.2
0.4
0.6
0.8
1
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
271.0 -> 140.0, 271.0 -> 165.0Not Found
+ MRM (4.853-6.853 min, 468 scans) (271.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 2x10
00.20.40.60.8
11.21.41.61.8
165.0
140.0 271.0
+ MRM (326.0 -> 291.0) Benzo Cal09.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
326.0 -> 291.0, 326.0 -> 249.0Not Found
+ MRM (5.375-7.375 min, 468 scans) (326.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)300 310 320 330 340 350
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
326.0
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Quantitative Analysis Sample Report
document.xlsx Page 42 of 48 Printed at: 05:52:08 on: 05/09/2023
diazepam+ MRM (285.0 -> 222.0) Benzo Cal09.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Not Found
+ MRM (5.477-7.477 min, 468 scans) (285.0 -> **) Benzo Cal09.d
Mass-to-Charge (m/z)260 265 270 275 280 285
Coun
ts 1x10
012345678
257.0
285.0
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Quantitative Analysis Sample Report
document.xlsx Page 43 of 48 Printed at: 05:52:08 on: 05/09/2023
Batch Info 2007-11-30T12:55:32.828125-08:00 2010-02-26T21:34:35.9969456-08:00 2007-11-30T12:55:31.796875-08:00
Batch Data Path C:\MassHunter\Data\BenzosInUrine\QuantResults\BenzosInUrine.batch.bin
Analysis Time 11/30/2007 12:55 PM Analyst Name mtischlerReport Time 2/26/2010 9:34 PM Reporter Name mtischlerLast Calib Update 11/30/2007 12:55 PM Batch State Processed
Analysis InfoData File Benzo Cal08.d Sample Name Benzo QCSample Type QC Dilution 1Acq Method Benzos Mix Unit Pos APCI Segs.m Acq Time 2007-03-08 11:51Position P1-F8 Inj Vol -1.00Comment Sample Info
Sample Chromatogram
Quantitation ResultsCompound ISTD RT Response ISTD Resp Resp Ratio Final Conc Units
7-amino clonazepam 0.982 17646 4228 4.1733 163.8184 ng/ml
1.180 4797 4228 1.1346 99.0015 ng/mlclonazepam D4-clonazepam 3.195 19686 8840 2.2269 95.4207 ng/mlflunitrazepam D4-clonazepam 3.272 42 8840 0.0047 3.1130 ng/mltriazolam D5-temazepam 3.901 4947 13759 0.3596 97.7468 ng/mloxazepam D5-oxazepam 4.171 20974 11134 1.8838 104.7185 ng/mlalprazolam D5-alprazolam 4.056 15140 7427 2.0386 93.0424 ng/mllorazepam D5-oxazepam 4.115 8260 11134 0.7419 52.9328 ng/ml
desalkylflurazepam D5-temazepam 4.541 8243 13759 0.5991 91.6483 ng/mltemazepam D5-temazepam 4.655 15199 13759 1.1047 98.9742 ng/mlchlordiazepoxide D5-nordiazepam 5.319 16 4054 0.0039 0.0000 ng/mlnordiazepam D5-nordiazepam 5.892 10 4054 0.0025 4.6528 ng/mlmidazolam D5-nordiazepam 6.325 4897 4054 1.2078 61.8378 ng/mldiazepam D5-nordiazepam 6.863 8 4054 0.0019 0.0000 ng/ml
D4-7-amino clonazepam
7-amino flunitrazepam
D4-7-amino clonazepam
+ TIC MRM (** -> **) Benzo Cal08.d
Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5
Coun
ts 4x10
-0.10
0.10.20.30.40.50.60.70.80.9
11.1
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Quantitative Analysis Sample Report
document.xlsx Page 44 of 48 Printed at: 05:52:08 on: 05/09/2023
Compound Graphics7-amino clonazepam
7-amino flunitrazepam
zopiclone
clonazepam
+ MRM (286.0 -> 121.0) Benzo Cal08.d
Acquisition Time (min)0.5 1 1.5
Coun
ts 3x10
0
1
2
3
4
5
7-amino clonazepam
Acquisition Time (min)0.5 1 1.5
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
286.0 -> 121.0, 286.0 -> 222.0Ratio = 75.8 (91.8 %)
+ MRM (0.929-1.166 min, 56 scans) (286.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)150 200 250
Coun
ts 4x10
0
1
2
3
4
5
6
121.0
222.0
286.0
+ MRM (284.0 -> 226.0) Benzo Cal08.d
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Coun
ts 3x10
00.20.40.60.8
11.21.4
7-amino flunitrazepam
Acquisition Time (min)0.4 0.6 0.8 1 1.2 1.4 1.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
284.0 -> 226.0, 284.0 -> 256.0Ratio = 72.8 (93.3 %)
+ MRM (1.123-1.310 min, 44 scans) (284.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 4x10
00.20.40.60.8
11.21.41.6
226.0
256.0
284.0
+ MRM (389.1 -> 245.1) Benzo Cal08.d
Acquisition Time (min)2 2.2 2.4 2.6
Coun
ts
0
1
2
3
4
5
6
7
Acquisition Time (min)2 2.2 2.4 2.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
389.1 -> 245.1, 389.1 -> 217.1Not Found
+ MRM (1.470-3.470 min, 468 scans) (389.1 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)250 300 350
Coun
ts 1x10
00.20.40.60.8
11.21.41.6
245.1
389.1
+ MRM (316.0 -> 270.0) Benzo Cal08.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 3x10
00.5
11.5
22.5
33.5
4clonazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
316.0 -> 270.0, 316.0 -> 214.0Ratio = 22.8 (99.2 %)
+ MRM (3.100-3.379 min, 66 scans) (316.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)220 240 260 280 300 320
Coun
ts 4x10
0
1
2
3
4
5
6270.0
214.0
316.0
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Quantitative Analysis Sample Report
document.xlsx Page 45 of 48 Printed at: 05:52:09 on: 05/09/2023
flunitrazepam
alpha-OH alprazolam
triazolam
oxazepam
+ MRM (314.0 -> 268.0) Benzo Cal08.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
00.250.5
0.751
1.251.5
1.75flunitrazepam
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
00.20.40.60.8
11.21.41.6
314.0 -> 268.0, 314.0 -> 239.0Ratio = 148.4 (297.6 %)
+ MRM (3.249-3.310 min, 15 scans) (314.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)240 260 280 300
Coun
ts 2x10
0
0.2
0.4
0.6
0.8
1
1.2
268.0
239.0
314.0
+ MRM (325.0 -> 297.0) Benzo Cal08.d
Acquisition Time (min)3 3.2 3.4 3.6
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3 3.2 3.4 3.6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
325.0 -> 297.0, 325.0 -> 216.0Not Found
+ MRM (2.517-4.517 min, 468 scans) (325.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)300 310 320 330 340 350
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
325.0
+ MRM (343.0 -> 308.0) Benzo Cal08.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
0.2
0.4
0.6
0.8
1triazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
343.0 -> 308.0, 343.0 -> 239.0Ratio = 90.1 (103.2 %)
+ MRM (3.816-4.005 min, 45 scans) (343.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)240 260 280 300 320 340
Coun
ts 4x10
0
0.2
0.4
0.6
0.8
1
1.2
1.4308.0
239.0
343.0
+ MRM (287.0 -> 241.0) Benzo Cal08.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.5
11.5
22.5
33.5
4
oxazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
287.0 -> 241.0, 287.0 -> 269.0Ratio = 31.1 (108.9 %)
+ MRM (4.049-4.291 min, 57 scans) (287.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)240 250 260 270 280
Coun
ts 4x10
0
1
2
3
4
5
241.0
269.0
287.0
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Quantitative Analysis Sample Report
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flurazepam
alprazolam
lorazepam
desalkylflurazepam
+ MRM (388.0 -> 315.0) Benzo Cal08.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
388.0 -> 315.0, 388.0 -> 288.0Not Found
+ MRM (3.025-5.025 min, 468 scans) (388.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)360 380 400 420
Coun
ts 1x10
00.10.20.30.40.50.60.70.8
388.0
+ MRM (309.0 -> 281.0) Benzo Cal08.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
0
0.5
1
1.5
2
2.5
alprazolam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
1.2
309.0 -> 281.0, 309.0 -> 274.0Ratio = 18.1 (117.1 %)
+ MRM (3.916-4.207 min, 69 scans) (309.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)280 290 300 310
Coun
ts 4x10
00.5
11.5
22.5
33.5
4
281.0
274.0
309.0
+ MRM (321.0 -> 275.0) Benzo Cal08.d
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Coun
ts 3x10
00.20.40.60.8
11.21.41.6
lorazepam
Acquisition Time (min)3.8 3.9 4 4.1 4.2 4.3
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
321.0 -> 275.0, 321.0 -> 229.0Ratio = 37.8 (98.8 %)
+ MRM (4.005-4.228 min, 53 scans) (321.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)240 260 280 300 320
Coun
ts 4x10
00.250.5
0.751
1.251.5
1.752
2.25275.0
229.0
321.0
+ MRM (289.0 -> 226.0) Benzo Cal08.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
00.20.40.6
0.81
1.21.4
desalkylflurazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
289.0 -> 226.0, 289.0 -> 261.0Ratio = 34.4 (119.8 %)
+ MRM (4.441-4.728 min, 68 scans) (289.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)240 260 280
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
3226.0
261.0
289.0
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Quantitative Analysis Sample Report
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temazepam
chlordiazepoxide
nordiazepam
midazolam
+ MRM (301.0 -> 255.0) Benzo Cal08.d
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Coun
ts 3x10
0
0.5
1
1.5
2
2.5
temazepam
Acquisition Time (min)4.4 4.5 4.6 4.7 4.8 4.9
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
301.0 -> 255.0, 301.0 -> 177.0Ratio = 31.3 (104.8 %)
+ MRM (4.539-4.843 min, 71 scans) (301.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)175 200 225 250 275 300
Coun
ts 4x10
0
1
2
3
4
5
255.0
177.0
301.0
+ MRM (300.0 -> 283.0) Benzo Cal08.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts 1x10
00.250.5
0.751
1.251.5
1.75
chlordiazepoxide
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 2x10
0
0.5
1
1.5
2
2.5
3
300.0 -> 283.0, 300.0 -> 227.0Not Found
+ MRM (5.288-5.355 min, 16 scans) (300.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)285 290 295 300
Coun
ts 1x10
00.5
11.5
22.5
33.5
4
283.0
300.0
+ MRM (271.0 -> 140.0) Benzo Cal08.d
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Coun
ts
0
1
2
3
4
5
nordiazepam
Acquisition Time (min)5.2 5.4 5.6 5.8 6
Rela
tive
Abun
danc
e (%
) 3x10
00.25
0.50.75
11.25
1.51.75
2
271.0 -> 140.0, 271.0 -> 165.0Ratio =
+ MRM (5.866-5.923 min, 14 scans) (271.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)150 175 200 225 250 275
Coun
ts 1x10
0
0.5
1
1.5
2
2.5
140.0
165.0
271.0
+ MRM (326.0 -> 291.0) Benzo Cal08.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts 2x10
01234567
midazolam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
326.0 -> 291.0, 326.0 -> 249.0Ratio = 30.8 (91.0 %)
+ MRM (6.218-6.468 min, 59 scans) (326.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)260 280 300 320
Coun
ts 4x10
0
0.5
1
1.5
2
2.5
291.0
249.0
326.0
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Quantitative Analysis Sample Report
document.xlsx Page 48 of 48 Printed at: 05:52:09 on: 05/09/2023
diazepam+ MRM (285.0 -> 222.0) Benzo Cal08.d
Acquisition Time (min)6.2 6.4 6.6 6.8
Coun
ts
0
1
2
3
4
5 diazepam
Acquisition Time (min)6.2 6.4 6.6 6.8
Rela
tive
Abun
danc
e (%
) 2x10
0
0.2
0.4
0.6
0.8
1
285.0 -> 222.0, 285.0 -> 257.0Not Found
+ MRM (6.856-6.885 min, 7 scans) (285.0 -> **) Benzo Cal08.d
Mass-to-Charge (m/z)220 240 260 280
Coun
ts 1x10
00.5
11.5
22.5
33.5
4222.0
285.0
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