WP2-P6-INRA LeafyVeg-26November2008 Activity of the P6 in the frame work of the project: regeneration of 300 lettuce accessions WP 2 – P6 (INRA-France)

LeafyVeg-26November2008WP2-P6-INRA

Activity of the P6 in the frame work of the project: regeneration of 300 lettuce accessions

WP 2 – P6 (INRA-France)

Objectives for year 2 the regeneration of 75 L. sativa

in year 1, regenerated seeds of 82 L. sativa have been included in the project


Work made at INRA in year 2

Regeneration of lettuce accessions

Control of the harvested seeds

Seed production under insect proof plastic tunnelStorage of seeds at 6°C

Uniformity and identity of the progenies

Insect-proof tunnel

Control of winter cultivars

Sample of seeds of the 82 accessions from 2007Safety duplication at Warwick


Regeneration of lettuce in 2008

Culture in 2008: 74 accessions (with 2 plants) Sowing of old seeds 22 February Plantation 26 March Notation of the minimum descriptors for

flowering Harvesting plant by plant of 69 cv from July to

August(> 10 g/plant)

Storage of harvested seeds at 6°C October

Insect-proof tunnel used for seed

production

4 of the dead accessions in summer regenerated in this fall in a glasshouse



Tunnel for multiplication in the end of April

Angurtana

Berenice

Arda

Barba di Frati

Plants on plastic

mulch, with stakes

Groups of 12 plants, corresponding to

6 accessions with 2 plants per cv



Tunnel for multiplication in the begining of May

Alicia

35 butterhead lettuce

Moskovskij



Tunnel for multiplication in the beginning of May

26 crisp lettuce

Red sails

Maravilla de Verano



Tunnel for multiplication in the beginning of May

Angurtana

Barba di Fratti

6 cos lettuce3 latin lettuce

4 cutting lettuce


Culture in field in 2008 : 35 accessions harvested in 2007

Control in 2008 of harvested seeds

Sowing 12 March Plantation 8 April Notation of the minimum descriptors for vegetative

characters and photos May

Culture in tunnel in 2008: 22 winter accessions harvested in 2008

Sowing 18 September Plantation 8 October Notation of the minimum descriptors for vegetative

characters and photos November-December


Field in May 2008

Total for the LeafyVeg project

Each accession: 2 plots of 20 plants

1 plot = progeny of one plant harvested

in insect-proof tunnel

in summer 2007

Other accessions are recent cultivars

27 crisp 1120 butterhead 135 cos 315 cutting lettuce 8


Control of summer lettuce

Photos for accession characterisation

Butterhead lettuce

Crisp lettuce Cos lettuce

Cutting lettuce

Cv Eynat

Cv Radichetta

Cv Dorémi

Cv Montemar

Cv Kinemontepas

Cv Kagraner


Winter tunnel in November 2008

Each accession: 2 plots of 32

plants

Other accessions are recent cultivars

11 crisp 722 butterhead 151 cos 1 cutting lettuce

Total for the LeafyVeg project


Control of winter Butterhead lettuce

Example with an old cultivar

Butterhead, cv Blondine

Observation of this plot

Cultigroup is conformNo wrong plant

Seed sample is good to include in the European

collection


Control of winter Butterhead lettuce

Examples : 2 modern cultivars from breeding companies

Batavia, cv Bacarès

Butterhead, cv Galore

In these plots :Cultigroup is conform

No wrong plant

Seed samples are good


Control of winter lettuce

Photos for accession characterisation

Cv Duran

The earliest butterhead

Cv Bristol Cv Isodor

Cv Pirouette

Cv Norden Cv Apollo


Safety duplication of 82 accessions (sent to Dave on April)

Regeneration of 73 accessions (69 in summer + 4 in fall) and storage of the seeds

Control of harvested seedsin open field = 35 field accessions harvested in 2007 under plastic tunnel = 22 winter accessions harvested in 2008

Notation of minimum descriptors and photos (in progress for winter accessions)

Conclusion of the year 2 for P6

Activities made in 2008


Copy in ILDB file of the minimum descriptors

Make a CD with photos of the plants at the heading stage if the parters are interested to put them on the ILDB Web site

Conclusion of the year 2 for P6

Work still to be doneActivities planned in LeafyVeg project

Storage at -18°C and safety duplication at Warwick of the seeds harvested in 2008

Other activities


Project for the year 3 for P6

Activities to made in 2009

At the end of the year 3

Regeneration of 74 accessions and storage of the seeds

Control of harvested seeds

Notation of minimum descriptors and photos

229 regenerated accessions out of the 300 in the project

Documents

WP2-P6-INRA LeafyVeg-26November2008 Activity of the P6 in the frame work of the project: regeneration of 300 lettuce accessions WP 2 – P6 (INRA-France)