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Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens PILLAR 1 and 2, WP13-wp25 Experimental procedure 1st Beef fillet-Inoculated 7th Beef fillet-Non inoculated 6th Beef fillet - Non inoculated 5th Beef fillet - Non inoculated 4th Beef fillet - Non inoculated 3rd Beef fillet - Non inoculated 2nd Beef fillet - Non inoculated Meat mincing machine 1st – 7th sample - Contaminated
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WP 13 and 25
PILLAR 2: Control and intervention strategies along the fork-to-farm chain to ensure beef safety
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
PILLAR 1 and 2, WP13-wp25
Experimental procedure Beef fillets (100 g)
Three pathogen bacteriao Listeria monocytogenes (mixture of 6 strains)o Salmonella ser. Typhimurium (mixture of 3 strains)o Escherichia coli 0157:H7 (mixture of 3 strains)
Three levels of inoculationo High level (107 CFU/g)o Medium level (105 CFU/g)o Low level (103 CFU/g)
Meat mincing machine
Two replications (from different beef batches) with three samples each
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
PILLAR 1 and 2, WP13-wp25
Experimental procedure
1st Beef fillet - Inoculated
7th Beef fillet - Non inoculated
6th Beef fillet - Non inoculated
5th Beef fillet - Non inoculated
4th Beef fillet - Non inoculated
3rd Beef fillet - Non inoculated
2nd Beef fillet - Non inoculated
Meat mincing machine
1st – 7th sample -
Contaminated
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
PILLAR 1 and 2, WP13-wp25
Conclusions till now… All the non-inoculated samples were contaminated from meat
mincing machine, for L. monocytogenes at all inoculum levels
A 2-log decrease was observed from 1st inoculated sample till 7th non-inoculated for the pathogen tested
In general, L. monocytogenes, Salm. ser. Typhimurium and E. coli may contaminate sequential meat samples through meat mincing machine and consist a high risk in processing industry
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
PILLAR 1 and 2, WP13-wp25
Conclusions till now…
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Samples
LogC
FU/g
L. monocytogenes contamination from meat mincing machine to 7 sequential beef samples for three initial inoculum levels
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
PILLAR 1 and 2, WP13-wp25
Future work (…to be done the next 1-2 months)
To finish with all the other pathogen bacteria
To replicate the experiment with different meat batch
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
PILLAR 1 and 2, WP13-wp25
Future work (…to be done the next 5-6 months)
Control of biofilm contamination at non-inoculated beef fillets (using microbiological and molecular techniques)
Three pathogen bacteriao Listeria monocytogenes (mixture of 6 strains)o Salmonella ser. Typhimurium (mixture of 3 strains)o Escherichia coli 0157:H7 (mixture of 3 strains)
Biofilm formation of these pathogens
Biofilm contamination of non-inoculated beef fillets
Laboratory of Microbiology & Biotechnology of Foods Agricultural University of Athens
PILLAR 1 and 2, WP13-wp25
Thank you
