Wound Healing Activity of Jasminum grandiflorum Linn.

M Syam Mohan*, J A J Sunilson, Y Hussein, J Thomas, T R Sekharan

*Presentation By:Y HusseinMasterskill College of Nursing and HealthMalaysia

Introduction Wound may be defined as loss or breaking of cellular and

anatomic or functional continuity of living tissue.

In the traditional system of medicine, the leaves of Jasminum

grandiflorum, which belongs to the family oleaceae are used

in the form of decoction against dermatitis and inflammation

in gums.

Objective

In the view of these, I have designed this present work to

study the possible effects of different extracts of the leaves of

Jasminum grandiflorum on wound healing, also knows as

Jasmin plant in Malaysia

A literature survey revealed that the plant leaves has not been

scientifically investigated for its wound healing activity.

The plant Jasminum grandiflorum

Materials

Collection:

• Trivandrum, Kerala, India in the

year 2003.

Study conducted at:

• S B. College of Pharmacy, Tamil

Nadu, India and repeated by a

student in Masterskill College

Method of study

Air dried leaves around 500 gm were reduced to coarse powder.

The leaves extracts were prepared in

Pet ether

Chloroform

Ethanol

Water

Apparatus used : Soxhlet apparatus

Base used : Simple oitment B.P

Animal Model : Excision model externally.

Qualitative Chemical Examination

Sl no

Plant constituents Extracts

Pet ether Chloroform Ethanol Aqueous

1 Alkaloids - + + +

2 Glycosides - - - +

3 Carbohydrates - - + +

4 Steroids - - + -

5 Tannins - - + -

6 Saponins - - + +

7 Flavanoids - - + +

8 Gums and mucilage - - + +

9 Fixed oil & Fat + - - -

10 Proteins - - - -

11 Volatile oils - - - -

‘+’ Present ‘-’ Absent

Thin layer chromatography – profile

The extracts were subjected to TLC.

Stationary phase: Silica gel-G

Saturation Time : 0.5 hr

Mobile phase : Benzene : Chloroform : Methanol

3 : 1 : 1

Spraying reagent : Dragendroff’s regent

Pharmacological screening

Animal used : Wistar Albino Rats

Groups : Total Six groups

Animal per group: Six

Excision wound model

Animals After infliction of wound

Effects of various extract Effect of PE ext of J. grandiflorum

0

100

200

300

400

500

600

0 2 4 6 8 10 12 14 16 18

Day

Dia

me

ter

of

wo

un

d

(mm

2)

Control Standard PE ext

Effect of CF ext of J. grandiflorum

0

100

200

300

400

500

600

0 2 4 6 8 10 12 14 16 18

Day

dia

met

er o

f w

ou

nd

(m

m2)

Control Standard CF ext

Effect of EtOH of J. grandiflorum

0

100

200

300

400

500

600

0 2 4 6 8 10 12 14 16 18

Day

dia

met

er o

f w

ou

nd

(m

m2)

Control Standard EtOH ext

Effect of aq. ext of J. grandiflorum

0

100

200

300

400

500

600

0 2 4 6 8 10 12 14 16 18

Day

dia

me

ter

of

wo

un

d

(mm

2)

Control Standard Aq ext

Animal after healing of wound

Discussion and conclusion

The results revealed that all extract showed a significant

wound contracting ability.

The pharmacological study substantiate the use of plant extract

as a wound healing agent.

This plant shows the activity owing to the presence of

alkaloids, steroids and tannins.

So necessary steps to be taken in future to identify the active

principles, study the toxicity and other pharmacological

property

Acknowledgement

S. B College of Pharmacy , India.

Masterskill college of nursing and health,

Malaysia.