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Wool Testing Grant
Reid Redden, Animal Science Department
Igathinathane Cannayen, Ag & Biosystems Engineering
Christopher Schauer, Hettinger Research Extension Center
Background
• ASI convention 2012
• Establish Collaborative Scientist
– C. Igathi
Background
• Cell Phone Camera – not good enough
• Scanner (19200 DPI – 1.3 micron
resolution) and software analysis
Preliminary Data
Preliminary Data
Standardized Specifications Scanner Results
Wool Grade
MicronRange
Standard Deviation
Initial FiberMicron
Initial Standard Deviation
CorrectionFactor
Corrected Fiber
Micron
Corrected Standard Deviation
70 17.70 - 19.14 4.59 51.59 14.59 2.53 20.43 5.78
62 22.05 - 23.49 5.89 71.44 11.49 2.53 28.28 4.55
60 23.50 - 24.94 6.49 54.24 13.97 2.53 21.47 5.53
58 24.95 - 26.39 7.09 62.18 13.35 2.53 24.61 5.29
56 26.40 - 27.84 7.59 71.44 16.68 2.53 28.28 6.6
54 27.85 - 29.29 8.19 76.73 12.58 2.53 30.38 4.98
48 31.00 - 32.69 9.09 82.02 13.16 2.53 32.47 5.21
Plan of Work
1. Increase Knowledge of Technology, Work
Environment, and Scanning Logistics –
Completed
Plan of Work
2. Possible system modification leading to
simpler layout (similar to laser instrument)
– but intelligent program evaluating from
fiber clusters of simpler layout
Time consuming layout Simpler layout
Plan of Work
2. Development of the system from verified
samples and test the system in various
work environments
Plan of Work
3. Publish the findings and present the new
system to the Wool Council
– Revision in 2013/2014
• NDSU Technology Transfer Office
– Patents/Licensing
– Commercialization of Products
• Work with Wool Council
Budget
• Transportation – $4,500
• Labor - $8,500
• Equipment - $6,000
• Publication - $1,000
• Indirects - $2,000
• Total - $22,000
Update of Tasks Completed
• Single fiber layout – plugin developed
• Multi fiber layout – initial plugin developed
• Steel wool fibers – measured
• Effect of weight in fiber layout – studied
• Calibration using microscope – completed
Single fiber layout
Single fiber layout – plugin results
Multiple fiber layout
Measures shape factors (area, roundness, solidity, and aspect ratio)Eliminates the overlap (e.g., segment 5)
Multiple fiber layout – plugin results
Sel_Area (1829) Sel_Round (0.70) Sel_Solidity (0.89) Sel_RAR(0.80)
Imaging with and without weights
Glass slides hold the fibers Weights applied on the ends of glass slide
Steel wool tested (12, 14, 22, and 26.6 micron)
Steel wool with and without weights – plugin test results
Additional wool tests with and without weights – plugin test results
Zeiss Imager M2 microscope
using 20x 0.8NA objective
ImagePro (MediaCybernetics)
software
Microscope and image processing
In focus central region
Ends out of focus
Micrograph calibration image – resolution: 1.115 micron; 22780 DPI
Processed and measured using plugin – caliper dimensions
With scale factor: 2.386 -> 34.651 38.422 36.690 0.831USDA Grade 31.96 micron
Results of microscope image analysis by plugin
Preprocessed scanner binary image – res: 1.323 micron; 19200 DPI
Measurements illustrated – res: 1.323 micron; 19200 DPI
Same fiber process by scanner and plugin
With scale factor: 2.386 -> 19.414 51.896 31.218 4.015USDA Grade 31.96 micron
Results of scanner image analysis by plugin
Single fibers width measurements using different techniques (microns)
Samples Microscope Plugin-Mic Plugin-Direct
WashWool-18.8 16.117 20.043 22.709
Grade 70s 21.444 21.271 23.786
WashWool-26.4 27.364 25.252 24.434
Grade 48s 39.590 36.907 31.962
0
5
10
15
20
25
30
35
40
45
WashWool-18.8 Grade 70s WashWool-26.4 Grade 48s
Fib
er
thic
kne
ss (
mic
ron
)
Samples
Microscope Plugin-Mic Plugin-Direct
Single fibers width measurement deviation from microscope (%)
Abs avg dev 9.92 20.45
Samples Plugin-Mic (%) Plugin-Direct (%)
WashWool-18.8 24.36 40.90
Grade 70s -0.80 10.92
WashWool-26.4 -7.72 -10.71
Grade 48s -6.78 -19.27
-30
-20
-10
0
10
20
30
40
50
WashWool-18.8 Grade 70s WashWool-26.4 Grade 48s
Fib
er
thic
kne
ss d
evia
tio
n f
rom
mic
rosc
op
e
(%)
Samples
Plugin-Mic (%) Plugin-Direct (%)
•Developed a method for calibrated samples
•Applying weight produces slightly better image
•Scanner methods were comparable to calibrated samples
• Field of view better for scanner
•Scanner is still a viable option
•About 1 minute process time – we will try to shorten it without affecting accuracy.
Conclusions
Status of Budget
• Salary - $0
• Travel - $3,000
• Publication - $1,000
• Equipment - $3,500
• I will take over as lead with Reid leaving
for TX.
Further Steps Required
• Test Multi-fiber Layout on Calibrated Samples
• Plugin improvement to tackle various scenarios (multi-fiber layout, curved fiber layout, orientation, etc.)
• Standardization of procedure
• Tests on on finely cut (chopped) wool – as additional method
• Deliver the 3 units at next year’s ASI annual convention.
Questions