WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

Graphs to show the effects of Inhibitors on Enzyme Action

3.2

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

1Rat

e o

f re

actio

n

Substrate concentration

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

Explanation

Forward

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

1

2

Rat

e o

f re

actio

n

Substrate concentration

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

ExplanationClose

Every active site is saturated. The rate of the reaction is at its highest.

Forward

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

1

2 3

Rat

e o

f re

actio

n

Substrate concentration

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

nForward

ExampleClose

Every active site is saturated, so there is no increase in the rate of reaction.

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

1

2 3

Competitive Inhibitor

Revise

Rat

e o

f re

actio

n

Substrate concentration

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

1

2

Non-competitive Inhibitor

Revise

Rat

e o

f re

actio

n

Substrate concentration

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

nForward

Close Explanation

A competitive inhibitor binds to the enzyme’s active site, and slows down the process.

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

ExplanationClose

The substrate will soon find an active site. The effect of a competitive inhibitor is to slow down the process.

Forward

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

1

2

Rat

e o

f re

actio

n

Revise

Substrate concentration

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

Close Explanation

The shape of the active site changes because the non-competitive inhibitor has bound to an allosteric site; so preventing an enzyme-substrate complex from forming.

Forward

WJE

C G

CE B

IOLO

GY

Inh

ibit

ors

an

d E

nzy

me A

ctio

n

Close Explanation

The reaction rate cannot reach the same point because the non-competitive inhibitors have changed the shape of some of the active sites.

Forward